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Asian-Australasian journal of animal sciences2017; 30(12); 1679-1683; doi: 10.5713/ajas.17.0133

The relationship between the variants in the 5′-untranslated regions of equine chorionic gonadotropin genes and serum equine chorionic gonadotropin levels.

Abstract: An experiment was conducted to study the association between the single nucleotide polymorphisms (SNPs) in 5'-untranslated regions (5'-UTR) of equine chorionic gonadotropin (eCG) genes and the serum eCG levels. Methods: SNPs in 5'-UTR of eCG genes were screened across 10 horse breeds, including 7 Chinese indigenous breeds and 3 imported breeds using iPLEX chemistry, and the association between the serum eCG levels of 174 pregnant Da'an mares and their serum eCG levels (determined with ELISA) was analyzed. Results: Four SNPs were identified in the 5'-UTR of the eCGα gene, and one of them was unique in the indigenous breeds. There were 2 SNPs detected at the 5' end of the eCGβ subunit gene, and one of them was only found in the Chinese breeds. The SNP g.39948246T>C at the 5'-UTR of eCGα was associated significantly with eCG levels of 75-day pregnant mare serum (pC mutation causes appearance of the specific binding site of hepatocyte nuclear factor 3 forkhead homolog 2 (HFH-2), which is a transcriptional repressor belonging to the forkhead protein family of transcription factors. Conclusions: The SNP g.39948246T>C at the 5'-UTR of eCGα is associated with eCG levels of 75-day pregnant mare serum (p<0.05).
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Publication Date: 2017-04-19 PubMed ID: 28423874PubMed Central: PMC5666169DOI: 10.5713/ajas.17.0133Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores the connection between variations in a specific region of horse genes associated with the production of a pregnancy hormone, and levels of this hormone in the bloodstream. The study found a particular genetic variance unique to certain indigenous horse breeds linked to higher hormone levels.

Methods

  • The study looked at single nucleotide polymorphisms (SNPs), which are variations in the smallest units of DNA, in a specific region of equine chorionic gonadotropin (eCG) genes. eCG is a hormone produced during pregnancy.
  • They screened for these SNPs in 10 horse breeds, 7 native to China and 3 imported breeds.
  • The researchers then assessed the relationship between these SNPs and the amount of eCG in the blood of 174 pregnant mares from the Da’an breed. Blood levels of eCG were determined using an ELISA test, which is a common laboratory method for measuring substances in blood samples.

Results

  • They found four SNPs in the 5′-untranslated regions (5′-UTR) of the eCGα gene, one of which was unique to the native breeds.
  • Two SNPs were found in the 5′-end region of the eCGβ subunit gene, one of which was only found in the Chinese breeds.
  • It was discovered that the SNP identified as g.39948246T>C in the 5′-UTR of the eCGα gene was significantly linked to eCG blood levels in mares who were 75 days pregnant.
  • The researchers also predicted that this SNP causes the creation of a unique binding site for a protein known as hepatocyte nuclear factor 3 forkhead homolog 2 (HFH-2), which suppresses gene activity. The appearance of this specific binding site might explain why this SNP impacts eCG levels.

Conclusion

  • The study concluded that this specific SNP (g.39948246T>C) in the eCGα gene is associated with the level of eCG hormone in the blood of mares that are 75 days pregnant.

Cite This Article

APA
Liu S, Lian S, Yang Y, Fu C, Ma H, Xiong Z, Ling Y, Zhao C. (2017). The relationship between the variants in the 5′-untranslated regions of equine chorionic gonadotropin genes and serum equine chorionic gonadotropin levels. Asian-Australas J Anim Sci, 30(12), 1679-1683. https://doi.org/10.5713/ajas.17.0133

Publication

Asian-Australasian journal of animal sciences
ISSN: 1011-2367
NlmUniqueID: 9884245
Country: Korea (South)
Language: English
Volume: 30
Issue: 12
Pages: 1679-1683

Researcher Affiliations

Liu, ShuQin
  • Equine Center, China Agricultural University, Beijing 100193, China.
Lian, Song
  • College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Yang, YunZhou
  • Equine Center, China Agricultural University, Beijing 100193, China.
Fu, ChunZheng
  • Equine Center, China Agricultural University, Beijing 100193, China.
Ma, HongYing
  • Equine Center, China Agricultural University, Beijing 100193, China.
Xiong, ZhiYao
  • Equine Center, China Agricultural University, Beijing 100193, China.
Ling, Yao
  • College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Zhao, ChunJiang
  • Equine Center, China Agricultural University, Beijing 100193, China.
  • College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
  • Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture, 100193, China.
  • National Engineering Laboratory for Animal Breeding, Beijing 100193, China.

Conflict of Interest Statement

. We certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript.

References

This article includes 21 references
  1. Cole HH, Hart GH. The potency of blood serum of mares in progressive stages of pregnancy in effecting the sexual maturity of the immature rat. Am J Physiol 1930;93:57–68.
  2. Cupps PT. Reproduction in domestic animals. Cambridge, MA, USA: Academic Press Inc; 1991. pp. 25–80.
  3. Bó GA, Baruselli PS. Synchronization of ovulation and fixed-time artificial insemination in beef cattle.. Animal 2014 May;8 Suppl 1:144-50.
    pubmed: 24844128doi: 10.1017/s1751731114000822google scholar: lookup
  4. Bó GA, Baruselli PS, Moreno D, Cutaia L, Caccia M, Tríbulo R, Tríbulo H, Mapletoft RJ. The control of follicular wave development for self-appointed embryo transfer programs in cattle.. Theriogenology 2002 Jan 1;57(1):53-72.
    pubmed: 11775981doi: 10.1016/s0093-691x(01)00657-4google scholar: lookup
  5. Sales JN, Crepaldi GA, Girotto RW, Souza AH, Baruselli PS. Fixed-time AI protocols replacing eCG with a single dose of FSH were less effective in stimulating follicular growth, ovulation, and fertility in suckled-anestrus Nelore beef cows.. Anim Reprod Sci 2011 Mar;124(1-2):12-8.
    pubmed: 21376482doi: 10.1016/j.anireprosci.2011.02.007google scholar: lookup
  6. Sá Filho MF, Ayres H, Ferreira RM, Marques MO, Reis EL, Silva RC, Rodrigues CA, Madureira EH, Bó GA, Baruselli PS. Equine chorionic gonadotropin and gonadotropin-releasing hormone enhance fertility in a norgestomet-based, timed artificial insemination protocol in suckled Nelore (Bos indicus) cows.. Theriogenology 2010 Mar 15;73(5):651-8.
    pubmed: 20080296doi: 10.1016/j.theriogenology.2009.11.004google scholar: lookup
  7. Baruselli PS, Reis EL, Marques MO, Nasser LF, Bó GA. The use of hormonal treatments to improve reproductive performance of anestrous beef cattle in tropical climates.. Anim Reprod Sci 2004 Jul;82-83:479-86.
    pubmed: 15271474doi: 10.1016/j.anireprosci.2004.04.025google scholar: lookup
  8. Cole HH, Goss H. The source of equine gonadotropin. Berkeley and Los Angeles: University of California Press; 1943; pp. 107–19.
  9. Allen WR. Factors influencing pregnant mare serum gonadotrophin production.. Nature 1969 Jul 5;223(5201):64-5.
    pubmed: 5792429doi: 10.1038/223064a0google scholar: lookup
  10. Lian S, Hou XL, Han HB, Han GC. Research advance in maternal level of eCG in pregnant mare. J Northeast Agric Univ 2010;41:157–60.
  11. Simoni M, Tüttelmann F, Michel C, Böckenfeld Y, Nieschlag E, Gromoll J. Polymorphisms of the luteinizing hormone/chorionic gonadotropin receptor gene: association with maldescended testes and male infertility.. Pharmacogenet Genomics 2008 Mar;18(3):193-200.
    pubmed: 18300940doi: 10.1097/fpc.0b013e3282f4e98cgoogle scholar: lookup
  12. Rull K, Nagirnaja L, Ulander VM, Kelgo P, Margus T, Kaare M, Aittomäki K, Laan M. Chorionic gonadotropin beta-gene variants are associated with recurrent miscarriage in two European populations.. J Clin Endocrinol Metab 2008 Dec;93(12):4697-706.
    pmc: PMC2737125pubmed: 18782867doi: 10.1210/jc.2008-1101google scholar: lookup
  13. Yang WC, Tang KQ, Li SJ, Chao LM, Yang LG. Polymorphisms of the bovine luteinizing hormone/choriogonadotropin receptor (LHCGR) gene and its association with superovulation traits.. Mol Biol Rep 2012 Mar;39(3):2481-7.
    pubmed: 21667104doi: 10.1007/s11033-011-0999-4google scholar: lookup
  14. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual, 2nd. New York: Cold Spring Harbor Laboratory Press; 1989.
  15. Wade CM, Giulotto E, Sigurdsson S, Zoli M, Gnerre S, Imsland F, Lear TL, Adelson DL, Bailey E, Bellone RR, Blöcker H, Distl O, Edgar RC, Garber M, Leeb T, Mauceli E, MacLeod JN, Penedo MC, Raison JM, Sharpe T, Vogel J, Andersson L, Antczak DF, Biagi T, Binns MM, Chowdhary BP, Coleman SJ, Della Valle G, Fryc S, Guérin G, Hasegawa T, Hill EW, Jurka J, Kiialainen A, Lindgren G, Liu J, Magnani E, Mickelson JR, Murray J, Nergadze SG, Onofrio R, Pedroni S, Piras MF, Raudsepp T, Rocchi M, Røed KH, Ryder OA, Searle S, Skow L, Swinburne JE, Syvänen AC, Tozaki T, Valberg SJ, Vaudin M, White JR, Zody MC, Lander ES, Lindblad-Toh K. Genome sequence, comparative analysis, and population genetics of the domestic horse.. Science 2009 Nov 6;326(5954):865-7.
    pmc: PMC3785132pubmed: 19892987doi: 10.1126/science.1178158google scholar: lookup
  16. Galet C, Le Bourhis CM, Chopineau M, Le Griec G, Perrin A, Magallon T, Attal J, Viglietta C, Houdebine LM, Guillou F. Expression of a single betaalpha chain protein of equine LH/CG in milk of transgenic rabbits and its biological activity.. Mol Cell Endocrinol 2001 Mar 28;174(1-2):31-40.
    pubmed: 11306169doi: 10.1016/s0303-7207(00)00452-4google scholar: lookup
  17. Legardinier S, Klett D, Poirier JC, Combarnous Y, Cahoreau C. Mammalian-like nonsialyl complex-type N-glycosylation of equine gonadotropins in Mimic insect cells.. Glycobiology 2005 Aug;15(8):776-90.
    pubmed: 15814822doi: 10.1093/glycob/cwi060google scholar: lookup
  18. Deng L. Secretory pattern, rapid analysis and raw blood harvest criterion for production of equine chorionic gonadotropin. Beijing, China: China Agriculture University; 2013.
  19. Latchman DS. Transcription factors: an overview.. Int J Biochem Cell Biol 1997 Dec;29(12):1305-12.
    pubmed: 9570129doi: 10.1016/s1357-2725(97)00085-xgoogle scholar: lookup
  20. Zhang Y, Wang Z, Xiao H, Liu X, Zhu G, Yu D, Han G, Chen G, Hou C, Ma N, Shen B, Li Y, Wang T, Wang R. Foxd3 suppresses interleukin-10 expression in B cells.. Immunology 2017 Apr;150(4):478-488.
    pmc: PMC5343362pubmed: 27995618doi: 10.1111/imm.12701google scholar: lookup
  21. He GY, Hu JL, Zhou L, Zhu XH, Xin SN, Zhang D, Lu GF, Liao WT, Ding YQ, Liang L. The FOXD3/miR-214/MED19 axis suppresses tumour growth and metastasis in human colorectal cancer.. Br J Cancer 2016 Nov 22;115(11):1367-1378.
    pmc: PMC5129822pubmed: 27811858doi: 10.1038/bjc.2016.362google scholar: lookup

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