The relationship between the variants in the 5′-untranslated regions of equine chorionic gonadotropin genes and serum equine chorionic gonadotropin levels.
Abstract: An experiment was conducted to study the association between the single nucleotide polymorphisms (SNPs) in 5'-untranslated regions (5'-UTR) of equine chorionic gonadotropin (eCG) genes and the serum eCG levels. Methods: SNPs in 5'-UTR of eCG genes were screened across 10 horse breeds, including 7 Chinese indigenous breeds and 3 imported breeds using iPLEX chemistry, and the association between the serum eCG levels of 174 pregnant Da'an mares and their serum eCG levels (determined with ELISA) was analyzed. Results: Four SNPs were identified in the 5'-UTR of the eCGα gene, and one of them was unique in the indigenous breeds. There were 2 SNPs detected at the 5' end of the eCGβ subunit gene, and one of them was only found in the Chinese breeds. The SNP g.39948246T>C at the 5'-UTR of eCGα was associated significantly with eCG levels of 75-day pregnant mare serum (pC mutation causes appearance of the specific binding site of hepatocyte nuclear factor 3 forkhead homolog 2 (HFH-2), which is a transcriptional repressor belonging to the forkhead protein family of transcription factors. Conclusions: The SNP g.39948246T>C at the 5'-UTR of eCGα is associated with eCG levels of 75-day pregnant mare serum (p<0.05).
Publication Date: 2017-04-19 PubMed ID: 28423874PubMed Central: PMC5666169DOI: 10.5713/ajas.17.0133Google Scholar: Lookup
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Summary
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This research explores the connection between variations in a specific region of horse genes associated with the production of a pregnancy hormone, and levels of this hormone in the bloodstream. The study found a particular genetic variance unique to certain indigenous horse breeds linked to higher hormone levels.
Methods
- The study looked at single nucleotide polymorphisms (SNPs), which are variations in the smallest units of DNA, in a specific region of equine chorionic gonadotropin (eCG) genes. eCG is a hormone produced during pregnancy.
- They screened for these SNPs in 10 horse breeds, 7 native to China and 3 imported breeds.
- The researchers then assessed the relationship between these SNPs and the amount of eCG in the blood of 174 pregnant mares from the Da’an breed. Blood levels of eCG were determined using an ELISA test, which is a common laboratory method for measuring substances in blood samples.
Results
- They found four SNPs in the 5′-untranslated regions (5′-UTR) of the eCGα gene, one of which was unique to the native breeds.
- Two SNPs were found in the 5′-end region of the eCGβ subunit gene, one of which was only found in the Chinese breeds.
- It was discovered that the SNP identified as g.39948246T>C in the 5′-UTR of the eCGα gene was significantly linked to eCG blood levels in mares who were 75 days pregnant.
- The researchers also predicted that this SNP causes the creation of a unique binding site for a protein known as hepatocyte nuclear factor 3 forkhead homolog 2 (HFH-2), which suppresses gene activity. The appearance of this specific binding site might explain why this SNP impacts eCG levels.
Conclusion
- The study concluded that this specific SNP (g.39948246T>C) in the eCGα gene is associated with the level of eCG hormone in the blood of mares that are 75 days pregnant.
Cite This Article
APA
Liu S, Lian S, Yang Y, Fu C, Ma H, Xiong Z, Ling Y, Zhao C.
(2017).
The relationship between the variants in the 5′-untranslated regions of equine chorionic gonadotropin genes and serum equine chorionic gonadotropin levels.
Asian-Australas J Anim Sci, 30(12), 1679-1683.
https://doi.org/10.5713/ajas.17.0133 Publication
Researcher Affiliations
- Equine Center, China Agricultural University, Beijing 100193, China.
- College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
- Equine Center, China Agricultural University, Beijing 100193, China.
- Equine Center, China Agricultural University, Beijing 100193, China.
- Equine Center, China Agricultural University, Beijing 100193, China.
- Equine Center, China Agricultural University, Beijing 100193, China.
- College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
- Equine Center, China Agricultural University, Beijing 100193, China.
- College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture, 100193, China.
- National Engineering Laboratory for Animal Breeding, Beijing 100193, China.
Conflict of Interest Statement
. We certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript.
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