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Home / Equine Research Bank / Can J Comp Med / The response of ponies to Myxovirus influenzae A-equi 2. II....
Canadian journal of comparative medicine : Revue canadienne de medecine comparee1970; 34(1); 7-12;

The response of ponies to Myxovirus influenzae A-equi 2. II. Immunoglobulin classes of antibody to the virus in serum and nasal secretions.

Abstract: Nasal secretions and serum were collected from ponies in order to determine the types of immunoglobulins responsible for antibody activity against Myxovirus influenzae A-equi 2. Using specific antisera to remove each immunoglobulin, the antiviral activity of serum was shown to be a property of IgG globulin, whereas in nasal secretions activity was found in both IgG and IgG(T) globulins. However in secretions the predominant activity was attributed to IgG(T) globulin. Because of its sensitivity to 2 mercaptoethanol, early serum antibody was assumed to belong to the IgM class of immunoglobulins. From its behaviour in sucrose density gradients and on Sephadex G 200 columns, the antibody in nasal secretions was assumed to be of 7S mobility not 11S such as is found in man.
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Publication Date: 1970-01-01 PubMed ID: 4192439PubMed Central: PMC1319413
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the role of different immunoglobulins in the immune response of ponies against a specific type of equine influenza virus. The main findings were that, in the blood serum, the immunoglobulin G (IgG) primary responsibility for the antiviral activity against the virus. In contrast, in the nasal secretions, immunoglobulin G and immunoglobulin G(T) display antiviral activity, with the latter showing more activity.

Background and Aim

  • The study was conducted to understand the immune response of ponies against Myxovirus influenzae A-equi 2, a type of equine influenza virus.
  • Most particularly, the focus of the analysis was on the immunoglobulins – a type of antibody – present in the blood and nasal secretions of the ponies.

Methods

  • Through a process called serum neutralization test, the researchers determined which immunoglobulins were responsible for virus antibody activity.
  • This involved adding specific antisera – blood serum containing antibodies – which would neutralize specific immunoglobulins in the ponies’ serum and nasal secretions.
  • Observing which antisera removed antibody activity would reveal which immunoglobulin was responsible for this activity.

Key Findings

  • The researchers found that in the ponies’ blood serum, antiviral activity was a property of IgG globulin.
  • In contrast, in the nasal secretions, both IgG and IgG(T) globulins showed antiviral activity.
  • However, the predominant activity in the nasal secretions was attributed to the IgG(T) globulin.

Additional Observations

  • The researchers observed that due to its sensitivity to 2 mercaptoethanol, a chemical compound, early serum antibody likely belonged to the immunoglobulin M (IgM) class.
  • They also surmised that the nasal secretion antibody was of 7S mobility, not 11S, which is typical in humans.
  • This was assumed based on how the antibodies behaved in sucrose density gradients and on Sephadex G 200 columns.

Implications of the Study

  • This research contributes to the understanding of the physiological response of ponies to the equine influenza virus.
  • It pinpoints the specific immunoglobulins involved in neutralizing the virus in blood serum and nasal secretions, important for understanding immunity in horses.
  • These results may aid in the development of more effective treatments or vaccines for equine influenza.

Cite This Article

APA
Rouse BT, Ditchfield WJ. (1970). The response of ponies to Myxovirus influenzae A-equi 2. II. Immunoglobulin classes of antibody to the virus in serum and nasal secretions. Can J Comp Med, 34(1), 7-12.

Publication

Canadian journal of comparative medicine : Revue canadienne de medecine comparee
ISSN: 0008-4050
NlmUniqueID: 0151747
Country: Canada
Language: English
Volume: 34
Issue: 1
Pages: 7-12

Researcher Affiliations

Rouse, B T
    Ditchfield, W J

      MeSH Terms

      • Animals
      • Antibodies / analysis
      • Antibody Formation / drug effects
      • Centrifugation, Density Gradient
      • Chromatography, Gel
      • Hemagglutination Inhibition Tests
      • Horses / immunology
      • Immunoglobulins / analysis
      • Influenza Vaccines / pharmacology
      • Mercaptoethanol / pharmacology
      • Neutralization Tests
      • Nose / analysis
      • Nose / immunology
      • Orthomyxoviridae / classification
      • Vaccination
      • gamma-Globulins / analysis

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      This article includes 21 references
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      Citations

      This article has been cited 3 times.
      1. Rouse BT, Babiuk LA. Host defense mechanisms against infectious bovine rhinotracheitis virus: in vitro stimulation of sensitized lymphocytes by virus antigen. Infect Immun 1974 Oct;10(4):681-7.
        doi: 10.1128/iai.10.4.681-687.1974pubmed: 4426702google scholar: lookup
      2. Widders PR, Stokes CR, David JS, Bourne FJ. Specific antibody in the equine genital tract following systemic and local immunization. Immunology 1985 Apr;54(4):763-9.
        pubmed: 3980048
      3. Virelizier JL, Oxford JS, Schild GC. The role of humoral immunity in host defence against influenza A infection in mice. Postgrad Med J 1976 Jun;52(608):332-7.
        doi: 10.1136/pgmj.52.608.332pubmed: 959113google scholar: lookup
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