The role of bicarbonate in the modulation of capacitation, spontaneous acrosome reaction and motility of equine fresh and frozen spermatozoa.
Abstract: In this study, we defined the composition of the culture medium that yield a significant percentage of alive and functional equine spermatozoa during enough time before artificial insemination. The effects of sodium bicarbonate were analyzed in fresh and frozen semen in respect to sperm viability, capacitation, spontaneous acrosome reaction and several kinetic parameters such as total motility, progressive motility, VCL, VSL, ALH, BCF, LIN. Moreover, employing Bayk-6844 and Nifedipine, the involvement of L-type voltage-gated calcium channels in the modulation of intracellular calcium concentrations was investigated. Results evidenced an immediate effect of sodium bicarbonate in reducing fresh sperm viability and in increasing capacitation and spontaneous acrosome reaction of fresh and frozen spermatozoa. Furthermore, it affected total and progressive motility of fresh and frozen semen. Because of the sudden effects of the compound, we think that a buffer lacking sodium bicarbonate is more suitable to preserve the viability and the functional state of equine spermatozoa required to undergo at the right time those modifications necessary for fertilization. We also demonstrated that intracellular calcium modifications induced by Bayk-8644 and Nifedipine are not involved in signals related to vitality, capacitation, spontaneous acrosome reaction and motility.
Copyright © 2022 Elsevier Inc. All rights reserved.
Publication Date: 2022-05-05 PubMed ID: 35561466DOI: 10.1016/j.theriogenology.2022.04.032Google Scholar: Lookup
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- Journal Article
Summary
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This research presents an exploration of the effects of sodium bicarbonate on horse sperm vitality and ability to fertilize. The study demonstrates that sodium bicarbonate has a negative impact on sperm health, suggesting that a buffer lacking this compound would be better for preserving the viability of horse sperm cells for artificial insemination.
Study Purpose and Methodology
- The purpose of this research was to determine the optimal composition of the culture medium for keeping equine sperm cells viable and functional for an extended period before artificial insemination.
- To conduct this study, sperm samples were collected and the effects of sodium bicarbonate were analyzed in both fresh and frozen semen.
- The researchers examined a series of parameters related to sperm vitality, including capacitation, spontaneous acrosome reaction, total motility, progressive motility, VCL, VSL, ALH, BCF, and LIN.
- In addition, the influence of L-type voltage-gated calcium channels on the modulation of intracellular calcium concentrations was explored using Bayk-6844 and Nifedipine.
Key Findings
- The study found that the presence of sodium bicarbonate immediately reduced the viability of fresh horse sperm and increased capacitation and spontaneous acrosome reaction in both fresh and frozen sperm samples.
- Beyond these effects, sodium bicarbonate also influenced the total and progressive mobility of fresh and frozen samples, further impairing sperm health.
- The researchers concluded that a buffer without sodium bicarbonate is likely more appropriate for maintaining the needed condition of equine sperm cells for successful fertilization.
Additional Observations
- This study also found that the changes in intracellular calcium concentrations induced by Bayk-8644 and Nifedipine did not affect vitality, capacitation, spontaneous acrosome reaction, or motility of the sperm cells.
- This indicates that calcium channel modifications are not involved in the vital signaling connected with these key sperm activities.
Cite This Article
APA
Albrizio M, Lacalandra GM, Cinone M.
(2022).
The role of bicarbonate in the modulation of capacitation, spontaneous acrosome reaction and motility of equine fresh and frozen spermatozoa.
Theriogenology, 187, 112-118.
https://doi.org/10.1016/j.theriogenology.2022.04.032 Publication
Researcher Affiliations
- Department of Emergency and Organs Transplantation, University of Bari Aldo Moro, Italy. Electronic address: maria.albrizio@uniba.it.
- Department of Veterinary Medicine, University of Bari Aldo Moro, Italy.
- Department of Emergency and Organs Transplantation, University of Bari Aldo Moro, Italy.
MeSH Terms
- Acrosome
- Acrosome Reaction
- Animals
- Bicarbonates
- Calcium / pharmacology
- Horses
- Male
- Nifedipine / pharmacology
- Sodium Bicarbonate / pharmacology
- Sperm Capacitation
- Sperm Motility
- Spermatozoa
Conflict of Interest Statement
Declaration of competing interest The authors declare no competing interests.
Citations
This article has been cited 2 times.- Breitbart H, Grinshtein E. Mechanisms That Protect Mammalian Sperm from the Spontaneous Acrosome Reaction. Int J Mol Sci 2023 Nov 30;24(23).
- Zhang L, Wang Y, Sun X, Kang Y, Sohail T, Wang J, Li Y. Effects of Different Diluents on Semen Quality of Hu Ram Stored at 4 °C. Animals (Basel) 2023 Sep 6;13(18).
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