The role of endothelial cell infection in the endometrium, placenta and foetus of equid herpesvirus 1 (EHV-1) abortions.
Abstract: One of three mares in the last trimester of pregnancy became paraplegic 7 days after experimental infection with EHV-1 and was killed 10 days after infection (d.p.i.). The other two mares aborted foetuses at 12 and 14 d.p.i. In the first mare, virus was detected by immunofluorescence (IIF) and immunoperoxidase (IP) staining in endothelial cells of the endometrium, placenta and umbilical vein, but not in any other foetal tissues. In the experimentally aborted foetuses, and in two other independent field cases of abortions, endothelial cell infection was also detected in the foetuses, both in major blood vessels and in capillaries or sinusoidal cells associated with parenchymal lesions. In these four cases there were also positive endothelial placental lesions detected by IIF or IP, although it was not always possible to isolate virus from these tissues, as it was from the foetuses. The evidence suggests that infection of maternal endothelial cells has a major role in the pathogenesis of abortion, and that endothelial cells are also involved in dissemination of virus within the foetus.
Publication Date: 1991-05-01 PubMed ID: 1651960DOI: 10.1016/s0021-9975(08)80148-xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study investigates how the infection of endothelial cells by the equid herpesvirus 1 (EHV-1) contributes to abortions in mares. The research primarily delves into how the EHV-1 virus affects the endometrium, placenta, and foetus, leading to paraplegia and abortion.
Experimental Investigation
- The researchers infected three pregnant mares with the EHV-1 virus. One mare became paraplegic seven days post-infection and was euthanized ten days after the infection. The other two mares aborted the foetuses at twelve and fourteen days post-infection.
- Virus identification in these mares was performed using immunofluorescence (IIF) and immunoperoxidase (IP) staining techniques. In the first mare that developed paraplegia, the virus was detected in endothelial cells of the endometrium, placenta, and umbilical vein. However, no presence of the virus was reported in other foetal tissues.
Analysis of Aborted Foetuses and Field Cases
- In the aborted foetuses from the experiment, as well as in two other independent field cases of abortions, endothelial cell infection was detected in the foetuses. In these instances, the virus was found in major blood vessels and in capillaries or sinusoidal cells associated with parenchymal lesions.
- Apart from the foetuses, the study also reported positive endothelial placental lesions detected by IIF or IP. It wasn’t always possible to isolate the virus from these tissues as it was from the foetuses.
Conclusions
- The study findings suggest that when EHV-1 infects maternal endothelial cells, the effects can lead to abortion, highlighting the significant role these cells play in the pathogenesis of abortion.
- Furthermore, these endothelial cells also appear to have a role in the spread of the virus within the foetus. A better understanding of these mechanisms can offer insights into managing and potentially preventing EHV-1 related abortions in mares.
Cite This Article
APA
Edington N, Smyth B, Griffiths L.
(1991).
The role of endothelial cell infection in the endometrium, placenta and foetus of equid herpesvirus 1 (EHV-1) abortions.
J Comp Pathol, 104(4), 379-387.
https://doi.org/10.1016/s0021-9975(08)80148-x Publication
Researcher Affiliations
- Department of Veterinary Pathology, Royal Veterinary College, London, U.K.
MeSH Terms
- Abortion, Septic / veterinary
- Abortion, Veterinary / microbiology
- Animals
- Antibodies, Viral / analysis
- Endometrium / microbiology
- Endothelium, Vascular / microbiology
- Female
- Fetus / microbiology
- Herpesviridae Infections / veterinary
- Herpesvirus 1, Equid / immunology
- Herpesvirus 1, Equid / isolation & purification
- Horse Diseases / microbiology
- Horse Diseases / transmission
- Horses
- Immunoenzyme Techniques
- Placenta / microbiology
- Pregnancy
- Umbilical Arteries / microbiology
- Umbilical Veins / microbiology
Citations
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