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Home / Equine Research Bank / Reprod Biol Endocrinol / The secretions of oviduct epithelial cells increase the equi...
Reproductive biology and endocrinology : RB&E2009; 7; 129; doi: 10.1186/1477-7827-7-129

The secretions of oviduct epithelial cells increase the equine in vitro fertilization rate: are osteopontin, atrial natriuretic peptide A and oviductin involved?

Abstract: Oviduct epithelial cells (OEC) co-culture promotes in vitro fertilization (IVF) in human, bovine and porcine species, but no data are available from equine species. Yet, despite numerous attempts, equine IVF rates remain low. Our first aim was to verify a beneficial effect of the OEC on equine IVF. In mammals, oviductal proteins have been shown to interact with gametes and play a role in fertilization. Thus, our second aim was to identify the proteins involved in fertilization in the horse. Results: In the first experiment, we co-incubated fresh equine spermatozoa treated with calcium ionophore and in vitro matured equine oocytes with or without porcine OEC. We showed that the presence of OEC increases the IVF rates. In the subsequent experiments, we co-incubated equine gametes with OEC and we showed that the IVF rates were not significantly different between 1) gametes co-incubated with equine vs porcine OEC, 2) intact cumulus-oocyte complexes vs denuded oocytes, 3) OEC previously stimulated with human Chorionic Gonadotropin, Luteinizing Hormone and/or oestradiol vs non stimulated OEC, 4) in vivo vs in vitro matured oocytes. In order to identify the proteins responsible for the positive effect of OEC, we first searched for the presence of the genes encoding oviductin, osteopontin and atrial natriuretic peptide A (ANP A) in the equine genome. We showed that the genes coding for osteopontin and ANP A are present. But the one for oviductin either has become a pseudogene during evolution of horse genome or has been not well annotated in horse genome sequence. We then showed that osteopontin and ANP A proteins are present in the equine oviduct using a surface plasmon resonance biosensor, and we analyzed their expression during oestrus cycle by Western blot. Finally, we co-incubated equine gametes with or without purified osteopontin or synthesized ANP A. No significant effect of osteopontin or ANP A was observed, though osteopontin slightly increased the IVF rates. Conclusions: Our study shows a beneficial effect of homologous and heterologous oviduct cells on equine IVF rates, though the rates remain low. Furthers studies are necessary to identify the proteins involved. We showed that the surface plasmon resonance technique is efficient and powerful to analyze molecular interactions during fertilization.
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Publication Date: 2009-11-19 PubMed ID: 19925651PubMed Central: PMC2785818DOI: 10.1186/1477-7827-7-129Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers studied the effects of oviduct epithelial cells (OEC) co-culture on in vitro fertilization (IVF) rates in horses, finding that their presence potentially improves IVF rates. They also looked for the genes and proteins thought to play a role in fertilization, particularly oviductin, osteopontin, and atrial natriuretic peptide A.

Objective of the Research

  • The research had two primary objectives. Firstly, to determine if the presence of OEC improved equine IVF rates, as has been observed in humans, cattle, and pigs. Equine IVF rates have traditionally remained low, making this an area of significant interest.
  • Secondly, the researchers aimed to identify proteins that may influence fertilization in horses. In mammals, certain proteins within the oviduct have been observed to interact with gametes and impact fertilization.

Research Method and Findings

  • In the initial experiment, researchers treated fresh horse spermatozoa with calcium ionophore and matured oocytes in vitro with or without porcine OEC. The presence of OEC appeared to increase IVF rates.
  • Subsequent experiments saw similar IVF rates, regardless of whether equine or porcine OEC were used, whether the cumulus-oocyte complexes were intact or denuded, whether the OEC were previously stimulated with human Chorionic Gonadotropin, Luteinizing Hormone, and/or oestradiol, and whether the oocytes matured in vivo or in vitro.
  • Seeking causative proteins, the researchers found the genes for osteopontin and atrial natriuretic peptide A (ANP A) in the equine genome. However, the oviductin gene may have become a pseudogene over the horse’s evolutionary history or could be poorly annotated in the equine genome sequence.
  • Researchers confirmed osteopontin and ANP A proteins are present in the equine oviduct using a surface plasmon resonance biosensor and analyzed their expression during oestrus cycle using Western blot. Co-incubation of equine gametes with purified osteopontin or synthesized ANP A did not significantly impact IVF rates, though osteopontin slightly increased the IVF rates.

Conclusions and Future Directions

  • The researchers concluded the presence of OEC—irrespective of whether they’re homologous or heterologous—might have a positive effect on equine IVF rates, though the rates remain comparatively low. They suggested further research to identify the specific proteins involved.
  • The research also demonstrated the efficacy of the surface plasmon resonance technique in analyzing molecular interactions during fertilization.

Cite This Article

APA
Mugnier S, Kervella M, Douet C, Canepa S, Pascal G, Deleuze S, Duchamp G, Monget P, Goudet G. (2009). The secretions of oviduct epithelial cells increase the equine in vitro fertilization rate: are osteopontin, atrial natriuretic peptide A and oviductin involved? Reprod Biol Endocrinol, 7, 129. https://doi.org/10.1186/1477-7827-7-129

Publication

Reproductive biology and endocrinology : RB&E
ISSN: 1477-7827
NlmUniqueID: 101153627
Country: England
Language: English
Volume: 7
Pages: 129

Researcher Affiliations

Mugnier, Sylvie
  • INRA, UMR85, Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France. sylvie.mugnier@tours.inra.fr
Kervella, Morgane
    Douet, Cécile
      Canepa, Sylvie
        Pascal, Géraldine
          Deleuze, Stefan
            Duchamp, Guy
              Monget, Philippe
                Goudet, Ghylène

                  MeSH Terms

                  • Animals
                  • Atrial Natriuretic Factor / genetics
                  • Atrial Natriuretic Factor / metabolism
                  • Atrial Natriuretic Factor / pharmacology
                  • Atrial Natriuretic Factor / physiology
                  • Body Fluids / metabolism
                  • Body Fluids / physiology
                  • Cells, Cultured
                  • Coculture Techniques
                  • Culture Media, Conditioned / metabolism
                  • Culture Media, Conditioned / pharmacology
                  • Epithelial Cells / metabolism
                  • Epithelial Cells / physiology
                  • Fallopian Tubes / metabolism
                  • Fallopian Tubes / physiology
                  • Female
                  • Fertilization in Vitro / drug effects
                  • Fertilization in Vitro / veterinary
                  • Horses / genetics
                  • Horses / metabolism
                  • Horses / physiology
                  • Male
                  • Osteopontin / genetics
                  • Osteopontin / metabolism
                  • Osteopontin / pharmacology
                  • Osteopontin / physiology
                  • Serine Endopeptidases / genetics
                  • Serine Endopeptidases / metabolism
                  • Serine Endopeptidases / physiology
                  • Swine
                  • Treatment Outcome

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