The seminal plasma proteins Peptidyl arginine deaminase 2, rRNA adenine N (6)-methyltransferase and KIAA0825 are linked to better motility post thaw in stallions.
Abstract: Seminal plasma plays an important role in sperm physiology. Seminal plasma proteins vehiculated in microvesicles, carry RNAs and proteins with a potential role in early embryo development. Additionally, proteins present in seminal plasma participate in redox regulation and energy metabolism. In view of these facts, we hypothesized that differences in protein composition of the seminal plasma among stallions may help to explain differences in freeze-ability seen among them. Three independent ejaculates from 10 different stallions of varying breeds were frozen using standard protocols in our laboratory. Aliquots of the ejaculate were separated and stored at -80 °C until further proteomic analysis. Semen analysis was performed using computer assisted sperm analysis and flow cytometry. Significant differences in proteome composition of seminal plasma were observed in the group of stallions showing better motility post thaw. 3116 proteins were identified, and of these, 34 were differentially expressed in stallions with better motility post thaw, 4 of them were also differentially expressed in stallions with different percentages of linearly motile sperm post thaw and 1 protein, Midasin, was expressed in stallions showing high circular velocity post thaw. Seminal plasma proteins may play a major role in sperm functionality; being vehiculated through extracellular vesicles and participating in sperm physiology. Bioinformatic analysis identifies discriminant proteins able to predict the outcome of cryopreservation, identifying potential new biomarkers to assess ejaculate quality.
Copyright © 2021 Elsevier Inc. All rights reserved.
Publication Date: 2021-10-14 PubMed ID: 34687941DOI: 10.1016/j.theriogenology.2021.10.010Google Scholar: Lookup
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- Journal Article
Summary
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The research studies the role of seminal plasma proteins in stallions’ sperm motility post-freeze. Three proteins were linked to better post-thaw motility in stallions, offering potential insights into sperm cryopreservation and quality assessment.
Study Overview
- The researchers posited that variations in the seminal plasma protein composition among stallions could account for the observed differences in sperm ‘freeze-ability’. To test this hypothesis, they ran several tests and analysis on ejaculates from ten stallions of varying breeds.
Methodology
- Three ejaculates were obtained independently from each stallion and frozen using standard protocols. Segments of these ejaculates were isolated and stored at -80°C for later proteomic analysis.
- Detailed semen analysis was carried out using computer-assisted sperm analysis (CASA) and flow cytometry. These are common technologies used for quantifying and characterizing sperm cells.
Findings
- Through their analysis, the researchers discovered significant variations in the proteome composition of the seminal plasma between groups of stallions showing better post-thaw motility.
- Out of 3116 proteins that were identified, 34 were differentially expressed in stallions with better motility post thaw. And of these, 4 were also differentially expressed in stallions with different percentages of linearly motile sperm post thaw.
- One protein, known as Midasin, was found to be expressed in stallions showing high circular velocity post thaw.
Implications
- These novel findings suggest that the proteins in seminal plasma could play a significant role in sperm functionality. The proteins are carried through extracellular vesicles, a process that can impact sperm physiology.
- Additionally, bioinformatic analysis has helped identify specific proteins that can anticipate the results of cryopreservation. These proteins could serve as potential new biomarkers to evaluate ejaculate quality, offering greater insights into fertility assessment and treatment.
Cite This Article
APA
Gaitskell-Phillips G, Martín-Cano FE, Ortiz-Rodríguez JM, Silva-Rodríguez A, da Silva-Álvarez E, Gil MC, Ortega-Ferrusola C, Peña FJ.
(2021).
The seminal plasma proteins Peptidyl arginine deaminase 2, rRNA adenine N (6)-methyltransferase and KIAA0825 are linked to better motility post thaw in stallions.
Theriogenology, 177, 94-102.
https://doi.org/10.1016/j.theriogenology.2021.10.010 Publication
Researcher Affiliations
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
- Facility of Innovation and Analysis in Animal Source Foodstuffs, University of Extremadura, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain. Electronic address: fjuanpvega@unex.es.
MeSH Terms
- Adenine
- Animals
- Arginine
- Cryopreservation / veterinary
- Horses
- Male
- Methyltransferases
- Proteomics
- Semen
- Semen Preservation / veterinary
- Seminal Plasma Proteins
- Sperm Motility
- Spermatozoa
Conflict of Interest Statement
Declaration of competing interest The authors have no conflicts of interest to disclose.
Citations
This article has been cited 2 times.- Gaitskell-Phillips G, Martín-Cano FE, Ortiz-Rodríguez JM, Silva-Rodríguez A, da Silva-Álvarez E, Gil MC, Ortega-Ferrusola C, Peña FJ. Dataset of the sperm proteome of stallions with different motility.. Data Brief 2022 Dec;45:108578.
- Vašíček J, Baláži A, Svoradová A, Vozaf J, Dujíčková L, Makarevich AV, Bauer M, Chrenek P. Comprehensive Flow-Cytometric Quality Assessment of Ram Sperm Intended for Gene Banking Using Standard and Novel Fertility Biomarkers.. Int J Mol Sci 2022 May 25;23(11).
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