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Comparative immunology, microbiology and infectious diseases2003; 26(4); 251-260; doi: 10.1016/S0147-9571(02)00054-1

The serologic response of horses to equine arteritis virus as determined by competitive enzyme-linked immunosorbent assays (c-ELISAs) to structural and non-structural viral proteins.

Abstract: In an effort to further characterize the humoral immune response of horses to equine arteritis virus (EAV), direct and competitive enzyme-linked immunosorbent assays (c-ELISAs) were developed using monoclonal and polyclonal anti-sera to structural (G(L), N and M) and non-structural (nsp1) viral proteins. A nsp1-specific monoclonal antibody was produced to facilitate development of a c-ELISA to this protein. Data obtained using the various c-ELISAs confirm that the M protein is a major target of the antibody response of horses to EAV. However, none of the c-ELISAs that were developed were as sensitive in detecting EAV-specific antibodies in horse sera as the existing serum neutralization test.
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Publication Date: 2003-04-05 PubMed ID: 12676125DOI: 10.1016/S0147-9571(02)00054-1Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study sought to further investigate the immune response of horses to equine arteritis virus (EAV) by developing direct and competitive enzyme-linked immunosorbent assays to identify structural and non-structural viral proteins. Though they were not as sensitive as existing methods, these new processes confirmed that the M protein is a major target of the horse’s immune response to EAV.

Development of New Assays

  • The research focused on improving the understanding of the horse’s immune system’s response to the equine arteritis virus (EAV).
  • An effort was made to develop direct and competitive enzyme-linked immunosorbent assays (c-ELISAs), which are laboratory tests widely used to detect and measure specific proteins or antibodies.
  • Both monoclonal and polyclonal antisera were used in these tests to pinpoint structural (G(L), N, and M) and non-structural (nsp1) viral proteins.
  • A unique monoclonal antibody targeting the non-structural protein nsp1 was also produced to facilitate the construction of a c-ELISA for this protein.

Findings and Conclusions

  • The data obtained using the different c-ELISAs showed that the M protein is a primary target of the horse’s antibody response to EAV.
  • This reaffirms the importance of the M protein within the virus and could be significant for furthering our understanding of the horse’s immune response mechanism to EAV.
  • However, despite these new developments, none of the newly designed c-ELISAs were as sensitive in detecting EAV-specific antibodies in horse serum as the pre-existing serum neutralization test.
  • This suggests that while the new c-ELISAs contribute to our understanding of the horse’s immune response to EAV, the development of even more sensitive tests in the future might still be necessary.

Cite This Article

APA
Wagner HM, Balasuriya UB, James MacLachlan N. (2003). The serologic response of horses to equine arteritis virus as determined by competitive enzyme-linked immunosorbent assays (c-ELISAs) to structural and non-structural viral proteins. Comp Immunol Microbiol Infect Dis, 26(4), 251-260. https://doi.org/10.1016/S0147-9571(02)00054-1

Publication

Comparative immunology, microbiology and infectious diseases
ISSN: 0147-9571
NlmUniqueID: 7808924
Country: England
Language: English
Volume: 26
Issue: 4
Pages: 251-260

Researcher Affiliations

Wagner, H M
  • Bernard and Gloria Salick Equine Viral Disease Laboratory, Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California at Davis, One Shields Avenue, 95616, Davis, CA, USA
Balasuriya, U B R
    James MacLachlan, N

      MeSH Terms

      • Animals
      • Antibodies, Monoclonal / immunology
      • Antibodies, Viral / immunology
      • Arterivirus Infections / immunology
      • Arterivirus Infections / veterinary
      • Cell Line
      • Cricetinae
      • Enzyme-Linked Immunosorbent Assay
      • Equartevirus / immunology
      • Horse Diseases / immunology
      • Horses / immunology
      • Viral Nonstructural Proteins / immunology
      • Viral Structural Proteins / immunology

      Citations

      This article has been cited 17 times.
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