The use of liquid chromatography tandem mass spectrometry to detect proteins in saliva from horses with and without systemic inflammation.
Abstract: The objective of the study was to assess global expression of proteins in equine saliva using liquid chromatography tandem mass spectrometry (LC-MS/MS). Saliva was obtained from seven horses with and six horses without evidence of systemic inflammatory disease. Tryptic peptides from saliva were analysed by LC-MS/MS. Of 195 unique proteins identified, 57 were detected only in saliva samples from horses with systemic inflammation (in two to six of the seven horses). Among the differentially expressed proteins were several acute phase proteins (APPs) such as serum amyloid A, fibrinogen, haptoglobin, and alpha1-acid glycoprotein. The study is the first to describe detection of inflammatory proteins in horse saliva. The proteins detected were similar to those described in saliva from cattle, small ruminants and pigs. Detection of APPs in horses with systemic inflammation suggests that saliva may be used for non-invasive disease monitoring in horses as in humans, pigs and dogs.
Copyright © 2014 Elsevier Ltd. All rights reserved.
Publication Date: 2014-09-16 PubMed ID: 25296850DOI: 10.1016/j.tvjl.2014.08.032Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research article explored the use of a detection method called liquid chromatography tandem mass spectrometry (LC-MS/MS) to identify proteins in horse saliva, specifically comparing healthy horses with those showing signs of systemic inflammation. The researchers were able to detect proteins linked to inflammation, suggesting these could possibly be used for non-invasive disease monitoring in horses.
Methodology
- The researchers collected saliva samples from seven horses showing evidence of systemic inflammation and six normally physiologically active horses.
- These saliva samples underwent an analysis process whereby they are broken down into tryptic peptides (chains of amino acids).
- The peptides were then analyzed on the LC-MS/MS. This is a chemistry technique that separates components in a liquid mixture (liquid chromatography) and then identifies and quantifies them (tandem mass spectrometry).
Findings
- Of the 195 unique proteins identified across all samples, 57 were only detected in the saliva samples from the horses showing signs of systemic inflammation.
- These 57 proteins include acute phase proteins (APPs) – a class of proteins whose plasma concentrations increase (positive acute-phase proteins) or decrease (negative acute-phase proteins) in response to inflammation. Examples of these found in the study include serum amyloid A, fibrinogen, haptoglobin, and alpha1-acid glycoprotein.
Implications
- This study presents the first account of detecting inflammatory proteins in horse saliva. Notably, these proteins showed similarity to those reportedly found in saliva samples from other animals including cattle, small ruminants, and pigs.
- The presence of APPs in the saliva of horses with systemic inflammation suggests that horse saliva may potentially be used as non-invasive means for disease monitoring.
- This echoes the utility of saliva for disease monitoring in human medicine, as well as in the veterinary care of pigs and dogs.
Cite This Article
APA
Jacobsen S, Top Adler DM, Bundgaard L, Sørensen MA, Andersen PH, Bendixen E.
(2014).
The use of liquid chromatography tandem mass spectrometry to detect proteins in saliva from horses with and without systemic inflammation.
Vet J, 202(3), 483-488.
https://doi.org/10.1016/j.tvjl.2014.08.032 Publication
Researcher Affiliations
- Department of Large Animal Sciences, Veterinary School of Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Hoejbakkegaard Allé 5, DK-2630 Taastrup, Denmark. Electronic address: stj@sund.ku.dk.
- Department of Large Animal Sciences, Veterinary School of Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Hoejbakkegaard Allé 5, DK-2630 Taastrup, Denmark.
- Department of Large Animal Sciences, Veterinary School of Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Hoejbakkegaard Allé 5, DK-2630 Taastrup, Denmark; Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds vej 10, DK-8000 Aarhus C, Denmark.
- Department of Large Animal Sciences, Veterinary School of Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Hoejbakkegaard Allé 5, DK-2630 Taastrup, Denmark.
- Department of Large Animal Sciences, Veterinary School of Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Hoejbakkegaard Allé 5, DK-2630 Taastrup, Denmark.
- Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds vej 10, DK-8000 Aarhus C, Denmark.
MeSH Terms
- Animals
- Chromatography, Liquid / veterinary
- Horse Diseases / immunology
- Horse Diseases / metabolism
- Horses
- Inflammation / immunology
- Inflammation / metabolism
- Inflammation / veterinary
- Proteome
- Saliva / chemistry
- Tandem Mass Spectrometry / veterinary
Citations
This article has been cited 8 times.- Adepu S, Lord M, Hugoh Z, Nyström S, Mattsson-Hulten L, Abrahamsson-Aurell K, Lützelschwab C, Skiöldebrand E. Salivary biglycan-neo-epitope-BGN(262): A novel surrogate biomarker for equine osteoarthritic sub-chondral bone sclerosis and to monitor the effect of short-term training and surface arena.. Osteoarthr Cartil Open 2023 Jun;5(2):100354.
- Contreras-Aguilar MD, Rubio CP, González-Arostegui LG, Martín-Cuervo M, Cerón JJ, Ayala I, Henriksen IH, Jacobsen S, Hansen S. Changes in Oxidative Status Biomarkers in Saliva and Serum in the Equine Gastric Ulcer Syndrome and Colic of Intestinal Aetiology: A Pilot Study.. Animals (Basel) 2022 Mar 7;12(5).
- Muñoz-Prieto A, Escribano D, Contreras-Aguilar MD, Horvatić A, Guillemin N, Jacobsen S, Cerón JJ, Mrljak V. Tandem Mass Tag (TMT) Proteomic Analysis of Saliva in Horses with Acute Abdominal Disease.. Animals (Basel) 2021 Apr 30;11(5).
- Minamijima Y, Niwa H, Uchida E, Yamamoto K. Comparison of the proteomes in sera between healthy Thoroughbreds and Thoroughbreds with respiratory disease associated with transport using mass spectrometry-based proteomics.. J Equine Sci 2021 Mar;32(1):11-15.
- Franco-Martínez L, Gelemanović A, Horvatić A, Contreras-Aguilar MD, Mrljak V, Cerón JJ, Martínez-Subiela S, Tvarijonaviciute A. The Serum and Saliva Proteome of Dogs with Diabetes Mellitus.. Animals (Basel) 2020 Dec 1;10(12).
- Bazzano M, Laghi L, Zhu C, Lotito E, Sgariglia S, Tesei B, Laus F. Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by (1)H-NMR.. Metabolites 2020 Jul 21;10(7).
- Long A, Nolen-Walston R. Equine Inflammatory Markers in the Twenty-First Century: A Focus on Serum Amyloid A.. Vet Clin North Am Equine Pract 2020 Apr;36(1):147-160.
- Contreras-Aguilar MD, Escribano D, Martínez-Subiela S, Martín-Cuervo M, Lamy E, Tecles F, Cerón JJ. Changes in saliva analytes in equine acute abdominal disease: a sialochemistry approach.. BMC Vet Res 2019 Jun 6;15(1):187.
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