The use of pentoxifylline to improve motility of cryopreserved equine spermatozoa.
Abstract: Pentoxifylline was evaluated as a method to increase motility of cryopreserved equine spermatozoa. In a preliminary experiment, pentoxifylline (3.5 mM or 7.0 mM) was added to extended semen that was chilled to 4 degrees C. Motility was evaluated at 8-h intervals for 48 h. The addition of 3.5 or 7.0 mM pentoxifylline appeared to increase the motility of chilled spermatozoa compared to controls. Based on these results, similar concentrations of pentoxifylline were added to semen either before or after cryopreservation. The addition of pentoxifylline (3.5 or 7.0 mM) to semen before cryopreservation significantly (P < 0.001) decreased total and progressive motility compared to controls. However, the addition of pentoxifylline (3.5 or 7.0 mM) to cryopreserved semen immediately after thawing significantly (P < 0.01) increased total and progressive motility compared to controls. These results indicate that pentoxifylline enhanced the postthaw motility of cryopreserved equine semen when added after thawing. Further research is required to evaluate the effect of pentoxifylline on the fertility of cryopreserved equine semen.
Publication Date: 2000-12-29 PubMed ID: 11131322DOI: 10.1016/s0093-691x(00)00412-xGoogle Scholar: Lookup
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- Journal Article
Summary
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The research explores enhancing the motility of frozen-thawed horse sperm using pentoxifylline. It was found that adding Pentoxifylline after freezing and thawing improved sperm movement.
Objective of the Research
- The aim of this research was to study the effects of the drug pentoxifylline on the mobility of cryopreserved (frozen and thawed) horse sperm. The study aimed to discover if pentoxifylline could be used to increase the mobility of the sperm, which could potentially increase fertility rates in horses.
Preliminary Experiments
- Initial investigations involved the addition of pentoxifylline to an extended sample of semen that was then chilled to 4 degrees Celsius. At 8-hour intervals over a 48-hour period, the motility of the semen was studied.
- It was observed that the addition of pentoxifylline seemed to improve the motility of the chilled sperm compared to the control sample that was not treated with the drug. This led to further exploration of the effects of Pentoxifylline on equine semen before and after cryopreservation.
Effects of Pentoxifylline on Cryopreserved Semen
- It was revealed that adding pentoxifylline to semen before it was cryogenically preserved resulted in a significant decrease in total motility and progressive motility compared to the control samples.
- Conversely, when pentoxifylline was added to the cryopreserved semen immediately after it was thawed, there was a significant increase in both total and progressive motility compared to the control samples. This suggests that Pentoxifylline is more effective when added after freezing and thawing.
Conclusion and Further Research
- The results of this research imply that pentoxifylline can improve the post-thaw motility of cryopreserved equine semen when it is added after thawing. The improvement of equine sperm motility after thawing could potentially enhance the semen’s fertility rate and improve the success of breeding programmes.
- However, it is necessary to conduct further research to evaluate and understand fully the effect of pentoxifylline on the fertility of cryopreserved equine semen.
Cite This Article
APA
Gradil CM, Ball BA.
(2000).
The use of pentoxifylline to improve motility of cryopreserved equine spermatozoa.
Theriogenology, 54(7), 1041-1047.
https://doi.org/10.1016/s0093-691x(00)00412-x Publication
Researcher Affiliations
- Department of Clinical Sciences, Cornell University, Ithaca, NY 14853, USA.
MeSH Terms
- Animals
- Cryopreservation
- Horses / physiology
- Male
- Pentoxifylline / pharmacology
- Phosphodiesterase Inhibitors / pharmacology
- Semen Preservation
- Sperm Motility / drug effects
Citations
This article has been cited 2 times.- Henkel RR, Schill WB. Sperm preparation for ART. Reprod Biol Endocrinol 2003 Nov 14;1:108.
- de Zutter BM, de Paula Freitas-Dell'Aqua C, Dell'Aqua-Junior JA, Monteiro GA, Troncarelli T, Papa FO. Optimising Stallion Semen Cryopreservation: Preliminary Insights Into Pre-Centrifugation Extender Effects. Reprod Domest Anim 2025 Oct;60(10):e70135.
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