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Home / Equine Research Bank / Parasit Vectors / Theileria equi isolates vary in susceptibility to imidocarb ...
Parasites & vectors2015; 8; 33; doi: 10.1186/s13071-014-0611-6

Theileria equi isolates vary in susceptibility to imidocarb dipropionate but demonstrate uniform in vitro susceptibility to a bumped kinase inhibitor.

Abstract: The apicomplexan hemoparasite Theileria equi is a causative agent of equine piroplasmosis, eradicated from the United States in 1988. However, recent outbreaks have sparked renewed interest in treatment options for infected horses. Imidocarb dipropionate is the current drug of choice, however variation in clinical response to therapy has been observed. Methods: We quantified the in vitro susceptibility of two T. equi isolates and a lab generated variant to both imidocarb dipropionate and a bumped kinase inhibitor compound 1294. We also evaluated the capacity of in vitro imidocarb dipropionate exposure to decrease susceptibility to that drug. The efficacy of imidocarb dipropionate for clearing infection in four T. equi infected ponies was also assessed. Results: We observed an almost four-fold difference in imidocarb dipropionate susceptibility between two distinct isolates of T. equi. Four ponies infected with the less susceptible USDA Florida strain failed to clear the parasite despite two rounds of treatment. Importantly, a further 15-fold decrease in susceptibility was produced in this strain by continuous in vitro imidocarb dipropionate exposure. Despite a demonstrated difference in imidocarb dipropionate susceptibility, there was no difference in the susceptibility of two T. equi isolates to bumped kinase inhibitor 1294. Conclusions: The observed variation in imidocarb dipropionate susceptibility, further reduction in susceptibility caused by drug exposure in vitro, and failure to clear T. equi infection in vivo, raises concern for the emergence of drug resistance in clinical cases undergoing treatment. Bumped kinase inhibitors may be effective as alternative drugs for the treatment of resistant T. equi parasites.
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Publication Date: 2015-01-20 PubMed ID: 25600252PubMed Central: PMC4311422DOI: 10.1186/s13071-014-0611-6Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • N.I.H.
  • Extramural
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article focuses on the susceptibility of a parasitic organism called Theileria equi, which causes equine piroplasmosis, to different treatments including imidocarb dipropionate and compound 1294. The results highlight variations in the effectiveness of these treatments, raising concerns about potential drug resistance.

Research Purpose and Methods

  • The main objective of this study was to observe and quantify the in vitro effectiveness of the drug imidocarb dipropionate and a kinase inhibitor, compound 1294, on two distinct Theileria equi isolates and a lab-generated variant. Another aim was also to evaluate the decrease in susceptibility of the parasite to imidocarb dipropionate after in vitro exposure.
  • The medicinal uptake capacity of imidocarb dipropionate was also evaluated by treating four horses infected with T. equi and analyzing the clearance of the parasite.

Research Findings

  • The researchers observed that the two distinct isolates of T. equi demonstrated an almost four-fold difference in susceptibility to the imidocarb dipropionate treatment. Notably, none of the horses treated with imidocarb dipropionate were able to clear the infection caused by the less susceptible USDA Florida strain, even after two rounds of treatment.
  • An interesting finding was that imidocarb dipropionate’s effectiveness was significantly reduced (further 15-fold) when the strain was exposed to it in vitro repeatedly. This denotes an increased resistance of the parasite to the drug upon repeated exposure.
  • The susceptibility of the T. equi isolates to the bumped kinase inhibitor 1294 treatment, on the other hand, showed no variance. This suggests that these parasites’ resistance may not build up against the kinase inhibitor as it does with imidocarb dipropionate.

Implications of the Study

  • The diverse susceptibility of T. equi to imidocarb dipropionate, as well as the reduction in susceptibility resulting from in vitro exposure, raises concerns about the development of drug resistance in the parasite during clinical treatments.
  • The invariable susceptibility of the parasites to the bumped kinase inhibitor 1294 suggests that it might be a viable alternative treatment, particularly in cases where resistance has been built up. However, this assertion would need further and thorough research to be validated.

Cite This Article

APA
Hines SA, Ramsay JD, Kappmeyer LS, Lau AO, Ojo KK, Van Voorhis WC, Knowles DP, Mealey RH. (2015). Theileria equi isolates vary in susceptibility to imidocarb dipropionate but demonstrate uniform in vitro susceptibility to a bumped kinase inhibitor. Parasit Vectors, 8, 33. https://doi.org/10.1186/s13071-014-0611-6

Publication

Parasites & vectors
ISSN: 1756-3305
NlmUniqueID: 101462774
Country: England
Language: English
Volume: 8
Pages: 33
PII: 33

Researcher Affiliations

Hines, Siddra A
  • Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, 99164-7040, USA. siddra@vetmed.wsu.edu.
Ramsay, Joshua D
  • Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, 99164-7040, USA. jdr105@vetmed.wsu.edu.
Kappmeyer, Lowell S
  • Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, 99164-7040, USA. lkapp@vetmed.wsu.edu.
  • Animal Disease Research Unit, Agricultural Research Service, USDA, Pullman, WA, 99164-6630, USA. lkapp@vetmed.wsu.edu.
Lau, Audrey Ot
  • Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, 99164-7040, USA. laua@vetmed.wsu.edu.
Ojo, Kayode K
  • Division of Allergy and Infectious Diseases and Center for Emerging and Re-emerging Infectious Diseases, School of Medicine, University of Washington, Seattle, WA, 98109-4766, USA. ojo67kk@u.washington.edu.
Van Voorhis, Wesley C
  • Division of Allergy and Infectious Diseases and Center for Emerging and Re-emerging Infectious Diseases, School of Medicine, University of Washington, Seattle, WA, 98109-4766, USA. WVanVoorhis@medicine.washington.edu.
Knowles, Donald P
  • Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, 99164-7040, USA. dknowles@vetmed.wsu.edu.
  • Animal Disease Research Unit, Agricultural Research Service, USDA, Pullman, WA, 99164-6630, USA. dknowles@vetmed.wsu.edu.
Mealey, Robert H
  • Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, 99164-7040, USA. rhm@vetmed.wsu.edu.

MeSH Terms

  • Amino Acid Sequence
  • Animals
  • Antiprotozoal Agents / therapeutic use
  • Cluster Analysis
  • Drug Resistance, Microbial / genetics
  • Flow Cytometry
  • Focal Adhesion Kinase 2 / antagonists & inhibitors
  • Horse Diseases / drug therapy
  • Horse Diseases / parasitology
  • Horses
  • Imidocarb / analogs & derivatives
  • Imidocarb / therapeutic use
  • Inhibitory Concentration 50
  • Molecular Sequence Data
  • Protein Kinase Inhibitors / therapeutic use
  • Sequence Alignment
  • Species Specificity
  • Theileria / genetics
  • Theileriasis / drug therapy
  • Theileriasis / epidemiology
  • Theileriasis / parasitology
  • United States / epidemiology

Grant Funding

  • R01 AI089441 / NIAID NIH HHS
  • R01 AI111341 / NIAID NIH HHS
  • 1 R01 AI089441 / NIAID NIH HHS
  • AI111341-01 / NIAID NIH HHS

References

This article includes 54 references
  1. Holbrook AA. Biology of equine piroplasmosis.. J Am Vet Med Assoc 1969 Jul 15;155(2):453-4.
    pubmed: 5816130
  2. Ribeiro IB, Câmara AC, Bittencourt MV, Marçola TG, Paludo GR, Soto-Blanco B. Detection of Theileria equi in spleen and blood of asymptomatic piroplasm carrier horses.. Acta Parasitol 2013 Jun;58(2):218-22.
    doi: 10.2478/s11686-013-0127-9pubmed: 23666659google scholar: lookup
  3. Ueti MW, Palmer GH, Kappmeyer LS, Statdfield M, Scoles GA, Knowles DP. Ability of the vector tick Boophilus microplus to acquire and transmit Babesia equi following feeding on chronically infected horses with low-level parasitemia.. J Clin Microbiol 2005 Aug;43(8):3755-9.
    doi: 10.1128/JCM.43.8.3755-3759.2005pmc: PMC1233951pubmed: 16081906google scholar: lookup
  4. Ueti MW, Mealey RH, Kappmeyer LS, White SN, Kumpula-McWhirter N, Pelzel AM, Grause JF, Bunn TO, Schwartz A, Traub-Dargatz JL, Hendrickson A, Espy B, Guthrie AJ, Fowler WK, Knowles DP. Re-emergence of the apicomplexan Theileria equi in the United States: elimination of persistent infection and transmission risk.. PLoS One 2012;7(9):e44713.
    doi: 10.1371/journal.pone.0044713pmc: PMC3435266pubmed: 22970295google scholar: lookup
  5. Committee on Infectious Diseases of Horses. Resolution #21: Equine Piroplasmosis - Release of Test Negative Treated Horses. United States Animal Health Association 115th Annual Meeting 2011. 11-1-2014.
  6. Scoles GA, Hutcheson HJ, Schlater JL, Hennager SG, Pelzel AM, Knowles DP. Equine piroplasmosis associated with Amblyomma cajennense Ticks, Texas, USA.. Emerg Infect Dis 2011 Oct;17(10):1903-5.
    doi: 10.3201/eid1710.101182pmc: PMC3310643pubmed: 22000367google scholar: lookup
  7. Frerichs WM, Allen PC, Holbrook AA. Equine piroplasmosis (Babesia equi): therapeutic trials of imidocarb dihydrochloride in horses and donkeys.. Vet Rec 1973 Jul 21;93(3):73-5.
    doi: 10.1136/vr.93.3.73pubmed: 4748680google scholar: lookup
  8. Kuttler KL, Zaugg JL, Gipson CA. Imidocarb and parvaquone in the treatment of piroplasmosis (Babesia equi) in equids.. Am J Vet Res 1987 Nov;48(11):1613-6.
    pubmed: 3434908
  9. Grause JF, Ueti MW, Nelson JT, Knowles DP, Kappmeyer LS, Bunn TO. Efficacy of imidocarb dipropionate in eliminating Theileria equi from experimentally infected horses.. Vet J 2013 Jun;196(3):541-6.
    doi: 10.1016/j.tvjl.2012.10.025pubmed: 23199699google scholar: lookup
  10. Knowles DP Jr. Control of Babesia equi parasitemia.. Parasitol Today 1996 May;12(5):195-8.
    doi: 10.1016/0169-4758(96)10007-7pubmed: 15275213google scholar: lookup
  11. Ding XC, Beck HP, Raso G. Plasmodium sensitivity to artemisinins: magic bullets hit elusive targets.. Trends Parasitol 2011 Feb;27(2):73-81.
    doi: 10.1016/j.pt.2010.11.006pubmed: 21169061google scholar: lookup
  12. Cohn LA, Birkenheuer AJ, Brunker JD, Ratcliff ER, Craig AW. Efficacy of atovaquone and azithromycin or imidocarb dipropionate in cats with acute cytauxzoonosis.. J Vet Intern Med 2011 Jan-Feb;25(1):55-60.
    doi: 10.1111/j.1939-1676.2010.0646.xpubmed: 21143646google scholar: lookup
  13. Lin EC, Chueh LL, Lin CN, Hsieh LE, Su BL. The therapeutic efficacy of two antibabesial strategies against Babesia gibsoni.. Vet Parasitol 2012 May 25;186(3-4):159-64.
    doi: 10.1016/j.vetpar.2011.11.073pubmed: 22222008google scholar: lookup
  14. Dahlström S, Aubouy A, Maïga-Ascofaré O, Faucher JF, Wakpo A, Ezinmègnon S, Massougbodji A, Houzé P, Kendjo E, Deloron P, Le Bras J, Houzé S. Plasmodium falciparum Polymorphisms associated with ex vivo drug susceptibility and clinical effectiveness of artemisinin-based combination therapies in Benin.. Antimicrob Agents Chemother 2014;58(1):1-10.
    doi: 10.1128/AAC.01790-12pmc: PMC3910716pubmed: 24100489google scholar: lookup
  15. Nagai A, Yokoyama N, Matsuo T, Bork S, Hirata H, Xuan X, Zhu Y, Claveria FG, Fujisaki K, Igarashi I. Growth-inhibitory effects of artesunate, pyrimethamine, and pamaquine against Babesia equi and Babesia caballi in in vitro cultures.. Antimicrob Agents Chemother 2003 Feb;47(2):800-3.
    doi: 10.1128/AAC.47.2.800-803.2003pmc: PMC151728pubmed: 12543697google scholar: lookup
  16. Wise LN, Ueti MW, Kappmeyer LS, Hines MT, White SN, Davis W, Knowles DP. In vitro activity of ponazuril against Theileria equi.. Vet Parasitol 2012 Apr 30;185(2-4):282-5.
    doi: 10.1016/j.vetpar.2011.10.036pubmed: 22130334google scholar: lookup
  17. Bork S, Yokoyama N, Matsuo T, Claveria FG, Fujisaki K, Igarashi I. Growth inhibitory effect of triclosan on equine and bovine Babesia parasites.. Am J Trop Med Hyg 2003 Mar;68(3):334-40.
    pubmed: 12685641
  18. Aboulaila M, Nakamura K, Govind Y, Yokoyama N, Igarashi I. Evaluation of the in vitro growth-inhibitory effect of epoxomicin on Babesia parasites.. Vet Parasitol 2010 Jan 20;167(1):19-27.
    doi: 10.1016/j.vetpar.2009.09.049pubmed: 19896277google scholar: lookup
  19. Munkhjargal T, AbouLaila M, Terkawi MA, Sivakumar T, Ichikawa M, Davaasuren B, Nyamjargal T, Yokoyama N, Igarashi I. Inhibitory effects of pepstatin A and mefloquine on the growth of Babesia parasites.. Am J Trop Med Hyg 2012 Oct;87(4):681-8.
    doi: 10.4269/ajtmh.2012.12-0218pmc: PMC3516319pubmed: 22890034google scholar: lookup
  20. Salama AA, AbouLaila M, Terkawi MA, Mousa A, El-Sify A, Allaam M, Zaghawa A, Yokoyama N, Igarashi I. Inhibitory effect of allicin on the growth of Babesia and Theileria equi parasites.. Parasitol Res 2014 Jan;113(1):275-83.
    doi: 10.1007/s00436-013-3654-2pubmed: 24173810google scholar: lookup
  21. Salama AA, Aboulaila M, Moussa AA, Nayel MA, El-Sify A, Terkawi MA, Hassan HY, Yokoyama N, Igarashi I. Evaluation of in vitro and in vivo inhibitory effects of fusidic acid on Babesia and Theileria parasites.. Vet Parasitol 2013 Jan 16;191(1-2):1-10.
    doi: 10.1016/j.vetpar.2012.08.022pubmed: 22985928google scholar: lookup
  22. Rodriguez RI, Trees AJ. In vitro responsiveness of Babesia bovis to imidocarb dipropionate and the selection of a drug-adapted line.. Vet Parasitol 1996 Mar;62(1-2):35-41.
    doi: 10.1016/0304-4017(95)00850-0pubmed: 8638391google scholar: lookup
  23. Preechapornkul P, Imwong M, Chotivanich K, Pongtavornpinyo W, Dondorp AM, Day NP, White NJ, Pukrittayakamee S. Plasmodium falciparum pfmdr1 amplification, mefloquine resistance, and parasite fitness.. Antimicrob Agents Chemother 2009 Apr;53(4):1509-15.
    doi: 10.1128/AAC.00241-08pmc: PMC2663078pubmed: 19164150google scholar: lookup
  24. Gaffar FR, Wilschut K, Franssen FF, de Vries E. An amino acid substitution in the Babesia bovis dihydrofolate reductase-thymidylate synthase gene is correlated to cross-resistance against pyrimethamine and WR99210.. Mol Biochem Parasitol 2004 Feb;133(2):209-19.
    doi: 10.1016/j.molbiopara.2003.08.013pubmed: 14698433google scholar: lookup
  25. Dalgliesh RJ, Stewart NP. Tolerance to imidocarb induced experimentally in tick-transmitted Babesia argentina.. Aust Vet J 1977 Apr;53(4):176-80.
    doi: 10.1111/j.1751-0813.1977.tb00163.xpubmed: 869813google scholar: lookup
  26. Doliwa C, Escotte-Binet S, Aubert D, Velard F, Schmid A, Geers R, Villena I. Induction of sulfadiazine resistance in vitro in Toxoplasma gondii.. Exp Parasitol 2013 Feb;133(2):131-6.
    doi: 10.1016/j.exppara.2012.11.019pubmed: 23206954google scholar: lookup
  27. Hwang SJ, Yamasaki M, Nakamura K, Sasaki N, Murakami M, Wickramasekara Rajapakshage BK, Ohta H, Maede Y, Takiguchi M. Development and characterization of a strain of Babesia gibsoni resistant to diminazene aceturate in vitro.. J Vet Med Sci 2010 Jun;72(6):765-71.
    doi: 10.1292/jvms.09-0535pubmed: 20160418google scholar: lookup
  28. Ojo KK, Pfander C, Mueller NR, Burstroem C, Larson ET, Bryan CM, Fox AM, Reid MC, Johnson SM, Murphy RC, Kennedy M, Mann H, Leibly DJ, Hewitt SN, Verlinde CL, Kappe S, Merritt EA, Maly DJ, Billker O, Van Voorhis WC. Transmission of malaria to mosquitoes blocked by bumped kinase inhibitors.. J Clin Invest 2012 Jun;122(6):2301-5.
    doi: 10.1172/JCI61822pmc: PMC3366411pubmed: 22565309google scholar: lookup
  29. Ojo KK, Eastman RT, Vidadala R, Zhang Z, Rivas KL, Choi R, Lutz JD, Reid MC, Fox AM, Hulverson MA, Kennedy M, Isoherranen N, Kim LM, Comess KM, Kempf DJ, Verlinde CL, Su XZ, Kappe SH, Maly DJ, Fan E, Van Voorhis WC. A specific inhibitor of PfCDPK4 blocks malaria transmission: chemical-genetic validation.. J Infect Dis 2014 Jan 15;209(2):275-84.
    doi: 10.1093/infdis/jit522pmc: PMC3873787pubmed: 24123773google scholar: lookup
  30. Doggett JS, Ojo KK, Fan E, Maly DJ, Van Voorhis WC. Bumped kinase inhibitor 1294 treats established Toxoplasma gondii infection.. Antimicrob Agents Chemother 2014 Jun;58(6):3547-9.
    doi: 10.1128/AAC.01823-13pmc: PMC4068437pubmed: 24687502google scholar: lookup
  31. Murphy RC, Ojo KK, Larson ET, Castellanos-Gonzalez A, Perera BG, Keyloun KR, Kim JE, Bhandari JG, Muller NR, Verlinde CL, White AC Jr, Merritt EA, Van Voorhis WC, Maly DJ. Discovery of Potent and Selective Inhibitors of Calcium-Dependent Protein Kinase 1 (CDPK1) from C. parvum and T. gondii.. ACS Med Chem Lett 2010 Oct 14;1(7):331-335.
    doi: 10.1021/ml100096tpmc: PMC2992447pubmed: 21116453google scholar: lookup
  32. Castellanos-Gonzalez A, White AC Jr, Ojo KK, Vidadala RS, Zhang Z, Reid MC, Fox AM, Keyloun KR, Rivas K, Irani A, Dann SM, Fan E, Maly DJ, Van Voorhis WC. A novel calcium-dependent protein kinase inhibitor as a lead compound for treating cryptosporidiosis.. J Infect Dis 2013 Oct 15;208(8):1342-8.
    doi: 10.1093/infdis/jit327pmc: PMC3778970pubmed: 23878324google scholar: lookup
  33. Keyloun KR, Reid MC, Choi R, Song Y, Fox AMW, Hillesland HK, Zhang Z, Vidadala R, Merritt EA, Lau AOT, Maly DJ, Fan E, Barrett LK, Van Voorhis WC, Ojo KK. The gatekeeper residue and beyond: homologous calcium-dependent protein kinases as drug development targets for veterinarian Apicomplexa parasites.. Parasitology 2014 Sep;141(11):1499-1509.
    doi: 10.1017/S0031182014000857pmc: PMC4390295pubmed: 24927073google scholar: lookup
  34. Larson ET, Ojo KK, Murphy RC, Johnson SM, Zhang Z, Kim JE, Leibly DJ, Fox AM, Reid MC, Dale EJ, Perera BG, Kim J, Hewitt SN, Hol WG, Verlinde CL, Fan E, Van Voorhis WC, Maly DJ, Merritt EA. Multiple determinants for selective inhibition of apicomplexan calcium-dependent protein kinase CDPK1.. J Med Chem 2012 Mar 22;55(6):2803-10.
    doi: 10.1021/jm201725vpmc: PMC3336864pubmed: 22369268google scholar: lookup
  35. Johnson SM, Murphy RC, Geiger JA, DeRocher AE, Zhang Z, Ojo KK, Larson ET, Perera BG, Dale EJ, He P, Reid MC, Fox AM, Mueller NR, Merritt EA, Fan E, Parsons M, Van Voorhis WC, Maly DJ. Development of Toxoplasma gondii calcium-dependent protein kinase 1 (TgCDPK1) inhibitors with potent anti-toxoplasma activity.. J Med Chem 2012 Mar 8;55(5):2416-26.
    doi: 10.1021/jm201713hpmc: PMC3306180pubmed: 22320388google scholar: lookup
  36. Knowles DP Jr, Perryman LE, Goff WL, Miller CD, Harrington RD, Gorham JR. A monoclonal antibody defines a geographically conserved surface protein epitope of Babesia equi merozoites.. Infect Immun 1991 Jul;59(7):2412-7.
    pmc: PMC258026pubmed: 1711016doi: 10.1128/iai.59.7.2412-2417.1991google scholar: lookup
  37. Kappmeyer LS, Thiagarajan M, Herndon DR, Ramsay JD, Caler E, Djikeng A, Gillespie JJ, Lau AO, Roalson EH, Silva JC, Silva MG, Suarez CE, Ueti MW, Nene VM, Mealey RH, Knowles DP, Brayton KA. Comparative genomic analysis and phylogenetic position of Theileria equi.. BMC Genomics 2012 Nov 9;13:603.
    doi: 10.1186/1471-2164-13-603pmc: PMC3505731pubmed: 23137308google scholar: lookup
  38. Holman PJ, Chieves L, Frerichs WM, Olson D, Wagner GG. Babesia equi erythrocytic stage continuously cultured in an enriched medium.. J Parasitol 1994 Apr;80(2):232-6.
    doi: 10.2307/3283752pubmed: 8158466google scholar: lookup
  39. Belloli C, Crescenzo G, Lai O, Carofiglio V, Marang O, Ormas P. Pharmacokinetics of imidocarb dipropionate in horses after intramuscular administration.. Equine Vet J 2002 Sep;34(6):625-9.
    doi: 10.2746/042516402776180124pubmed: 12358005google scholar: lookup
  40. Brasseur P, Lecoublet S, Kapel N, Favennec L, Ballet JJ. In vitro evaluation of drug susceptibilities of Babesia divergens isolates.. Antimicrob Agents Chemother 1998 Apr;42(4):818-20.
    pmc: PMC105548pubmed: 9559789doi: 10.1128/aac.42.4.818google scholar: lookup
  41. Naidoo V, Zweygarth E, Eloff JN, Swan GE. Identification of anti-babesial activity for four ethnoveterinary plants in vitro.. Vet Parasitol 2005 Jun 10;130(1-2):9-13.
    doi: 10.1016/j.vetpar.2005.03.001pubmed: 15893064google scholar: lookup
  42. Ramsay JD, Ueti MW, Johnson WC, Scoles GA, Knowles DP, Mealey RH. Lymphocytes and macrophages are infected by Theileria equi, but T cells and B cells are not required to establish infection in vivo.. PLoS One 2013;8(10):e76996.
    doi: 10.1371/journal.pone.0076996pmc: PMC3792048pubmed: 24116194google scholar: lookup
  43. Knowles DP Jr, Perryman LE, Kappmeyer LS, Hennager SG. Detection of equine antibody to Babesia equi merozoite proteins by a monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay.. J Clin Microbiol 1991 Sep;29(9):2056-8.
    pmc: PMC270260pubmed: 1774334doi: 10.1128/jcm.29.9.2056-2058.1991google scholar: lookup
  44. Nicolaiewsky TB, Richter MF, Lunge VR, Cunha CW, Delagostin O, Ikuta N, Fonseca AS, da Silva SS, Ozaki LS. Detection of Babesia equi (Laveran, 1901) by nested polymerase chain reaction.. Vet Parasitol 2001 Oct 31;101(1):9-21.
    doi: 10.1016/S0304-4017(01)00471-Xpubmed: 11587829google scholar: lookup
  45. Wang Z, Li X, Su D, Li Y, Wu L, Wang Y, Wu W. Residue depletion of imidocarb in Swine tissue.. J Agric Food Chem 2009 Mar 25;57(6):2324-8.
    doi: 10.1021/jf803251jpubmed: 19243130google scholar: lookup
  46. Lai O, Belloli C, Crescenzo G, Carofiglio V, Ormas P, Marangi O, Cagnardi P. Depletion and bioavailability of imidocarb residues in sheep and goat tissues.. Vet Hum Toxicol 2002 Apr;44(2):79-83.
    pubmed: 11931508
  47. Aliu YO, Davis RH Jr, Camp BJ, Kuttler KL. Absorption, distribution, and excretion of imidocarb dipropionate in sheep.. Am J Vet Res 1977 Dec;38(12):2001-7.
    pubmed: 596699
  48. Coldham NG, Moore AS, Dave M, Graham PJ, Sivapathasundaram S, Lake BG, Sauer MJ. Imidocarb residues in edible bovine tissues and in vitro assessment of imidocarb metabolism and cytotoxicity.. Drug Metab Dispos 1995 Apr;23(4):501-5.
    pubmed: 7600919
  49. Kerber CE, Ferreira F, Pereira MC. Control of equine piroplasmosis in Brazil.. Onderstepoort J Vet Res 1999 Jun;66(2):123-7.
    pubmed: 10486829
  50. Davis WC, Wyatt CR, Hamilton MJ, Goff WL. A rapid, reliable method of evaluating growth and viability of intraerythrocytic protozoan hemoparasites using fluorescence flow cytometry.. Mem Inst Oswaldo Cruz 1992;87 Suppl 3:235-9.
    doi: 10.1590/S0074-02761992000700039pubmed: 1343696google scholar: lookup
  51. Coetzee JF, Apley MD, Kocan KM, Jones DE. Flow cytometric evaluation of selected antimicrobial efficacy for clearance of Anaplasma marginale in short-term erythrocyte cultures.. J Vet Pharmacol Ther 2006 Jun;29(3):173-83.
    doi: 10.1111/j.1365-2885.2006.00734.xpubmed: 16669861google scholar: lookup
  52. Fukata T, Ohnishi T, Okuda S, Sasai K, Baba E, Arakawa A. Detection of canine erythrocytes infected with Babesia gibsoni by flow cytometry.. J Parasitol 1996 Aug;82(4):641-2.
    doi: 10.2307/3283793pubmed: 8691376google scholar: lookup
  53. Bicalho KA, Ribeiro MF, Martins-Filho OA. Molecular fluorescent approach to assessing intraerythrocytic hemoprotozoan Babesia canis infection in dogs.. Vet Parasitol 2004 Nov 10;125(3-4):221-35.
    doi: 10.1016/j.vetpar.2004.08.009pubmed: 15482880google scholar: lookup
  54. Sobolewski P, Gramaglia I, Frangos JA, Intaglietta M, van der Heyde H. Plasmodium berghei resists killing by reactive oxygen species.. Infect Immun 2005 Oct;73(10):6704-10.
    doi: 10.1128/IAI.73.10.6704-6710.2005pmc: PMC1230976pubmed: 16177347google scholar: lookup

Citations

This article has been cited 19 times.
  1. Onzere CK, Hulbert M, Sears KP, Williams LBA, Fry LM. Tulathromycin and Diclazuril Lack Efficacy against Theileria haneyi, but Tulathromycin Is Not Associated with Adverse Clinical Effects in Six Treated Adult Horses. Pathogens 2023 Mar 14;12(3).
    doi: 10.3390/pathogens12030453pubmed: 36986375google scholar: lookup
  2. Yang G, Chen K, Guo W, Hu Z, Qi T, Liu D, Wang Y, Du C, Wang X. Development of a Test Card Based on Colloidal Gold Immunochromatographic Strips for Rapid Detection of Antibodies against Theileria equi and Babesia caballi. Microbiol Spectr 2022 Feb 23;10(1):e0241121.
    doi: 10.1128/spectrum.02411-21pubmed: 35196786google scholar: lookup
  3. Tuvshintulga B, Nugraha AB, Mizutani T, Liu M, Ishizaki T, Sivakumar T, Xuan X, Yokoyama N, Igarashi I. Development of a stable transgenic Theileria equi parasite expressing an enhanced green fluorescent protein/blasticidin S deaminase. Sci Rep 2021 Apr 27;11(1):9107.
    doi: 10.1038/s41598-021-88594-wpubmed: 33907262google scholar: lookup
  4. Sears K, Knowles D, Dinkel K, Mshelia PW, Onzere C, Silva M, Fry L. Imidocarb Dipropionate Lacks Efficacy against Theileria haneyi and Fails to Consistently Clear Theileria equi in Horses Co-Infected with T. haneyi. Pathogens 2020 Dec 10;9(12).
    doi: 10.3390/pathogens9121035pubmed: 33321715google scholar: lookup
  5. Elsworth B, Duraisingh MT. A framework for signaling throughout the life cycle of Babesia species. Mol Microbiol 2021 May;115(5):882-890.
    doi: 10.1111/mmi.14650pubmed: 33274587google scholar: lookup
  6. Tirosh-Levy S, Gottlieb Y, Fry LM, Knowles DP, Steinman A. Twenty Years of Equine Piroplasmosis Research: Global Distribution, Molecular Diagnosis, and Phylogeny. Pathogens 2020 Nov 8;9(11).
    doi: 10.3390/pathogens9110926pubmed: 33171698google scholar: lookup
  7. Hines SA, Brandvold J, Mealey RH, Call DR, Graça T. Exposure to ambient air causes degradation and decreased in vitro potency of buparvaquone and parvaquone. Vet Parasitol X 2020 May;3:100023.
    doi: 10.1016/j.vpoa.2020.100023pubmed: 32904749google scholar: lookup
  8. Batiha GE, Beshbishy AM, Alkazmi LM, Nadwa EH, Rashwan EK, Yokoyama N, Igarashi I. In vitro and in vivo growth inhibitory activities of cryptolepine hydrate against several Babesia species and Theileria equi. PLoS Negl Trop Dis 2020 Aug;14(8):e0008489.
    doi: 10.1371/journal.pntd.0008489pubmed: 32853247google scholar: lookup
  9. O'Connor RM, Nepveux V FJ, Abenoja J, Bowden G, Reis P, Beaushaw J, Bone Relat RM, Driskell I, Gimenez F, Riggs MW, Schaefer DA, Schmidt EW, Lin Z, Distel DL, Clardy J, Ramadhar TR, Allred DR, Fritz HM, Rathod P, Chery L, White J. A symbiotic bacterium of shipworms produces a compound with broad spectrum anti-apicomplexan activity. PLoS Pathog 2020 May;16(5):e1008600.
    doi: 10.1371/journal.ppat.1008600pubmed: 32453775google scholar: lookup
  10. Beshbishy AM, Batiha GE, Alkazmi L, Nadwa E, Rashwan E, Abdeen A, Yokoyama N, Igarashi I. Therapeutic Effects of Atranorin towards the Proliferation of Babesia and Theileria Parasites. Pathogens 2020 Feb 17;9(2).
    doi: 10.3390/pathogens9020127pubmed: 32079149google scholar: lookup
  11. Silva MG, Villarino NF, Knowles DP, Suarez CE. Assessment of Draxxin(®) (tulathromycin) as an inhibitor of in vitro growth of Babesia bovis, Babesia bigemina and Theileria equi. Int J Parasitol Drugs Drug Resist 2018 Aug;8(2):265-270.
    doi: 10.1016/j.ijpddr.2018.04.004pubmed: 29689532google scholar: lookup
  12. Gimenez F, Hines SA, Evanoff R, Ojo KK, Van Voorhis WC, Maly DJ, Vidadala RSR, Mealey RH. In vitro growth inhibition of Theileria equi by bumped kinase inhibitors. Vet Parasitol 2018 Feb 15;251:90-94.
    doi: 10.1016/j.vetpar.2017.12.024pubmed: 29426483google scholar: lookup
  13. Tuvshintulga B, AbouLaila M, Sivakumar T, Tayebwa DS, Gantuya S, Naranbaatar K, Ishiyama A, Iwatsuki M, Otoguro K, Ōmura S, Terkawi MA, Guswanto A, Rizk MA, Yokoyama N, Igarashi I. Chemotherapeutic efficacies of a clofazimine and diminazene aceturate combination against piroplasm parasites and their AT-rich DNA-binding activity on Babesia bovis. Sci Rep 2017 Oct 24;7(1):13888.
    doi: 10.1038/s41598-017-14304-0pubmed: 29066849google scholar: lookup
  14. Van Voorhis WC, Doggett JS, Parsons M, Hulverson MA, Choi R, Arnold SLM, Riggs MW, Hemphill A, Howe DK, Mealey RH, Lau AOT, Merritt EA, Maly DJ, Fan E, Ojo KK. Extended-spectrum antiprotozoal bumped kinase inhibitors: A review. Exp Parasitol 2017 Sep;180:71-83.
    doi: 10.1016/j.exppara.2017.01.001pubmed: 28065755google scholar: lookup
  15. Ojo KK, Dangoudoubiyam S, Verma SK, Scheele S, DeRocher AE, Yeargan M, Choi R, Smith TR, Rivas KL, Hulverson MA, Barrett LK, Fan E, Maly DJ, Parsons M, Dubey JP, Howe DK, Van Voorhis WC. Selective inhibition of Sarcocystis neurona calcium-dependent protein kinase 1 for equine protozoal myeloencephalitis therapy. Int J Parasitol 2016 Dec;46(13-14):871-880.
    doi: 10.1016/j.ijpara.2016.08.003pubmed: 27729271google scholar: lookup
  16. Pedroni MJ, Vidadala RS, Choi R, Keyloun KR, Reid MC, Murphy RC, Barrett LK, Van Voorhis WC, Maly DJ, Ojo KK, Lau AO. Bumped kinase inhibitor prohibits egression in Babesia bovis. Vet Parasitol 2016 Jan 15;215:22-8.
    doi: 10.1016/j.vetpar.2015.10.023pubmed: 26790733google scholar: lookup
  17. Cardillo NN, Villarino NF, Kappmeyer LS, Chung CJ, Suarez CE, Bastos RG. Tafenoquine succinate inhibits the growth of the equine piroplasmosis hemoparasites Theileria equi and Babesia caballi. Parasit Vectors 2026 Jan 27;19(1):61.
    doi: 10.1186/s13071-026-07262-ypubmed: 41593688google scholar: lookup
  18. Mendoza FJ, Pérez-Écija A, Kappmeyer LS, Suarez CE, Bastos RG. New insights in the diagnosis and treatment of equine piroplasmosis: pitfalls, idiosyncrasies, and myths. Front Vet Sci 2024;11:1459989.
    doi: 10.3389/fvets.2024.1459989pubmed: 39205808google scholar: lookup
  19. Cardillo NM, Lacy PA, Villarino NF, Doggett JS, Riscoe MK, Bastos RG, Laughery JM, Ueti MW, Suarez CE. Comparative efficacy of buparvaquone and imidocarb in inhibiting the in vitro growth of Babesia bovis. Front Pharmacol 2024;15:1407548.
    doi: 10.3389/fphar.2024.1407548pubmed: 38751779google scholar: lookup
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