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Reproduction in domestic animals = Zuchthygiene2021; 56(3); 459-466; doi: 10.1111/rda.13884

Timed artificial insemination in crossbred mares: Reproductive efficiency and costs.

Abstract: Timed artificial insemination (TAI) has boosted the use of conventional artificial insemination (CAI) by employing hormonal protocols to synchronize oestrus and ovulation. This study aimed to evaluate the efficiency of a hormonal protocol for TAI in mares, based on a combination of progesterone releasing intravaginal device (PRID), prostaglandin (PGF2α ) and human chorionic gonadotropin (hCG); and compare financial costs between CAI and TAI. Twenty-one mares were divided into two groups: CAI group (CAIG; n = 6 mares; 17 oestrous cycles) and TAI group (TAIG; n = 15 mares; 15 oestrous cycles). The CAIG was subjected to CAI, involving follicular dynamics and uterine oedema monitoring with ultrasound examinations (US), and administration of hCG (1,600 IU) when the dominant follicle (DF) diameter's ≥35 mm + uterine oedema + cervix opening. The AI was performed with fresh semen (500 × 106 cells), and embryo was recovered on day 8 (D8) after ovulation. In TAI, mares received 1.9 g PRID on D0. On D10, PRID was removed and 6.71 mg dinoprost tromethamine was administered. Ovulation was induced on D14 (1,600 IU of hCG) regardless of the DF diameter's, and AI was performed with fresh semen (500 × 106 cells). On D30 after AI, pregnancy was confirmed by US. The pregnancy rate was 80.0% in TAIG and 82.3% in CAIG (p > .05). The TAI protocol resulted in 65% reduction in professional transport costs, and 40% reduction in material costs. The TAI was as efficient as CAI, provided reduction in costs and handlings, and is recommended in mares.
© 2020 Wiley-VCH GmbH.
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Publication Date: 2021-01-05 PubMed ID: 33368635DOI: 10.1111/rda.13884Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study examines the efficacy and financial implications of timed artificial insemination (TAI) in mares compared to conventional artificial insemination (CAI). The researchers found that TAI, which uses a hormonal protocol, is as successful as CAI for equine reproduction and can substantially reduce costs.

Study Design and Objective

  • The goal of the research was to assess the efficiency of timed artificial insemination (TAI) in mares. This was done using a hormone-based procedure involving a progesterone-releasing intravaginal device (PRID), prostaglandin, and human chorionic gonadotropin (hCG).
  • The experiment compared the financial costs related to TAI and conventional artificial insemination (CAI).

Participants and Methods

  • Twenty-one mares participated in the study and were divided into two groups: the TAI group (TAIG) and the CAI group (CAIG).
  • In the CAI process, subjects underwent ultrasound examinations for follicular dynamics and uterine edema monitoring. When the dominant follicle’s diameter was more than 35mm, hCG was administrated followed by artificial insemination with fresh semen. Embryos were harvested on the 8th day after ovulation.
  • In the TAI process, mares were given a progesterone-releasing device on day 0. It was removed on day 10 and a certain amount of dinoprost tromethamine was given. On day 14, ovulation was induced with hCG, followed by artificial insemination with fresh semen. Pregnancy was confirmed through ultrasound on day 30.

Results

  • The outcomes were favorable for both the TAI and CAI groups as the pregnancy rates were similar, 80.0% for TAI and 82.3% for CAI.
  • The TAI protocol led to a significant cost reduction, specifically a 65% decrease in professional transport expenses and a 40% reduction in material costs.

Conclusions and Implications

  • The study concluded that TAI is as effective as CAI in inducing pregnancy in mares.
  • Due to its cost-effectiveness and reduced handling requirements, timed artificial insemination is recommended.

Cite This Article

APA
Macan RC, Camargo CE, Zielinski BL, Cardoso NGH, de Lara NSS, Bergstein-Galan TG, Weiss RR, Kozicki LE. (2021). Timed artificial insemination in crossbred mares: Reproductive efficiency and costs. Reprod Domest Anim, 56(3), 459-466. https://doi.org/10.1111/rda.13884

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 56
Issue: 3
Pages: 459-466

Researcher Affiliations

Macan, Rodrigo Chaves
  • Post Graduate Program, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, Brazil.
Camargo, Carlos Eduardo
  • Post Graduate Program, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, Brazil.
Zielinski, Bruna Lampe
  • Post Graduate Program, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, Brazil.
Cardoso, Nathália Gonçalves Hesketh
  • Post Graduate Program, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, Brazil.
de Lara, Natália Santana Siqueira
  • Veterinary Sciences Department, Federal University of Paraná, Curitiba, Brazil.
Bergstein-Galan, Tácia Gomes
  • Faculty of Veterinary Medicine, Universidade Positivo, Curitiba, Brazil.
Weiss, Romildo Romualdo
  • Veterinary Sciences Department, Federal University of Paraná, Curitiba, Brazil.
Kozicki, Luiz Ernandes
  • Post Graduate Program, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, Brazil.

MeSH Terms

  • Administration, Intravaginal
  • Animals
  • Chorionic Gonadotropin / administration & dosage
  • Dinoprost / administration & dosage
  • Dinoprost / analogs & derivatives
  • Embryo Transfer
  • Estrus Synchronization / drug effects
  • Estrus Synchronization / methods
  • Female
  • Horses / embryology
  • Horses / physiology
  • Insemination, Artificial / economics
  • Insemination, Artificial / methods
  • Insemination, Artificial / veterinary
  • Male
  • Pregnancy
  • Pregnancy Rate
  • Progesterone / administration & dosage
  • Uterus / diagnostic imaging

Grant Funding

  • 001 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

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Citations

This article has been cited 2 times.
  1. Wysokińska A. Animal Reproduction: Semen Quality Assessment, Volume II. Animals (Basel) 2025 Mar 1;15(5).
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  2. Zhang L, Wang X, Jiang C, Sohail T, Sun Y, Sun X, Wang J, Li Y. Effect of Different Dilution Methods and Ratios of Ram Semen on Sperm Parameters after Cryopreservation. Animals (Basel) 2024 Mar 15;14(6).
    doi: 10.3390/ani14060907pubmed: 38540005google scholar: lookup
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