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Drug testing and analysis2021; 13(5); 1001-1007; doi: 10.1002/dta.3021

Towards an untargeted mass spectrometric approach for improved screening in equine antidoping.

Abstract: The emergence of novel doping agents is a continuous issue for analysts who aim to maintain the integrity of horseracing together with the well-being and safety of the animals and riders involved. Untargeted mass spectrometric analysis presents a potential improvement for antidoping as it enables the detection of compounds being indirectly affected by an administered drug. In this study, liquid chromatography-high-resolution mass spectrometry was used to investigate a 12-horse administration study of the synthetic opioid, butorphanol. A mass spectrometric workflow capable of detecting metabolic differences for an extended period of time was successfully developed. This proof-of-concept study demonstrates the potential of untargeted workflows to provide a list of biomarkers of exposure and effect that are indicative of drug administration which may be implemented into routine testing for improved doping control.
© 2021 John Wiley & Sons, Ltd.
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Publication Date: 2021-03-03 PubMed ID: 33629815DOI: 10.1002/dta.3021Google Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores the use of untargeted mass spectrometric analysis for improving equine antidoping procedures by detecting metabolic differences due to drug administration over an extended period.

Scope of the Research

  • Analysts continually face the issue of emerging novel doping agents, translating into challenges in maintaining the integrity of horseracing and ensuring the safety and well-being of the animals and riders involved.
  • This research proposes untargeted mass spectrometric analysis as a method to enhance the detection of doping in horses.

Technology Implemented

  • The study involved the use of liquid chromatography and high-resolution mass spectrometry for assessing the response of 12 horses to the administration of the synthetic opioid, butorphanol.
  • The mass spectrometry technology was aimed at detecting metabolic variances spanning an extensive timeframe.

Outcome and Implications

  • The successful development of the spectrometric workflow showcased its capability to pick up metabolic differences over a sustained period.
  • The research serves as a proof-of-concept study, underlining the potential of using untargeted workflows to yield a set of biomarkers indicative of drug administration.
  • The findings of the paper suggest that the approach pursued in this research can be incorporated into routine testing for an improved doping control mechanism.

Cite This Article

APA
Keen B, Cawley A, Fouracre C, Pyke J, Fu S. (2021). Towards an untargeted mass spectrometric approach for improved screening in equine antidoping. Drug Test Anal, 13(5), 1001-1007. https://doi.org/10.1002/dta.3021

Publication

Drug testing and analysis
ISSN: 1942-7611
NlmUniqueID: 101483449
Country: England
Language: English
Volume: 13
Issue: 5
Pages: 1001-1007

Researcher Affiliations

Keen, Bethany
  • Centre for Forensic Science, University of Technology Sydney, Sydney, New South Wales, Australia.
Cawley, Adam
  • Australian Racing Forensic Laboratory, Racing NSW, Sydney, New South Wales, Australia.
Fouracre, Chris
  • Agilent Technologies, Mulgrave, Victoria, Australia.
Pyke, James
  • Agilent Technologies, Santa Clara, California, USA.
Fu, Shanlin
  • Centre for Forensic Science, University of Technology Sydney, Sydney, New South Wales, Australia.

MeSH Terms

  • Analgesics, Opioid / blood
  • Animals
  • Butorphanol / blood
  • Chromatography, High Pressure Liquid / veterinary
  • Data Mining
  • Doping in Sports
  • Horses / blood
  • Male
  • Mass Spectrometry / veterinary
  • Proof of Concept Study
  • Reproducibility of Results
  • Substance Abuse Detection / veterinary
  • Workflow

Grant Funding

  • Australian Government Research Training Program Scholarship

References

This article includes 24 references
  1. Lowry LK. Role of biomarkers of exposure in the assessment of health risks.. Toxicol Lett 1995;77(1):31-38.
  2. Nordberg GF. Biomarkers of exposure, effects and susceptibility in humans and their application in studies of interactions among metals in China.. Toxicol Lett 2010;192(1):45-49.
  3. Teale P, Barton C, Driver PM, Kay RG. Biomarkers: unrealized potential in sports doping analysis.. Bioanalysis 2009;1(6):1103-1118.
  4. Narduzzi L, Dervilly G, Audran M, Le Bizec B, Buisson C. A role for metabolomics in the antidoping toolbox?. Drug Test Anal 2020;12(6):677-690.
  5. Dettmer K, Aronov PA, Hammock BD. Mass spectrometry-based metabolomics.. Mass Spectrom Rev 2007;26(1):51-78.
  6. Chan GHM, Ho ENM, Leung DKK, Wong KS, Wan TSM. Targeted metabolomics approach to detect the misuse of steroidal aromatase inhibitors in equine sports by biomarker profiling.. Anal Chem 2016;88(1):764-772.
  7. Authorities IFOH. International agreement on breeding, racing and wagering.. France: International Federation Of Horseracing Authorities; 2018:110.
  8. Cawley AT, Keledjian J. Intelligence-based anti-doping from an equine biological passport.. Drug Test Anal 2017;9(9):1441-1447.
  9. Tobin T. Horses running happy: fentanyl, morphine, and the other narcotic analgesics.. In: Drugs and the Performance Horse. Charles C Thomas; 1981:199-215.
  10. Pachter IJ, Evens RP. Butorphanol.. Drug Alcohol Depend 1985;14(3):325-338.
  11. Beaumier P, Young I. Butorphanol levels in the plasma and urine of the horse following intramuscular injection.. Control of the Use of Drugs in Racehorses 1983:186-191.
  12. Tavakoli M, Corssen G, Butorphanol CFS. Morphine: a double-blind comparison of their parenteral analgesic activity.. Anesthesia & Analgesia 1976;55(3):394-401.
  13. Pittman KA, Smyth RD, Mayol RF. Serum levels of butorphanol by radioimmunoassay.. J Pharm Sci 1980;69(2):160-163.
  14. Heel RC, Brogden RN, Speight TM, Avery GS. Butorphanol: a review of its pharmacological properties and therapeutic efficacy.. Drugs 1978;16(6):473-505.
  15. Sanchez LC, Robertson SA. Pain control in horses: what do we really know?. Equine Vet J 2014;46(4):517-523.
  16. Andraus MH, Siqueira MEPB. Determination of butorphanol in horse race urine by immunoassay and gas chromatography-mass spectrometry.. J Chromatogr B Biomed Sci Appl 1997;704(1):143-150.
  17. Mama KR, Hector RC. Therapeutic developments in equine pain management.. The Veterinary Journal 2019;247:50-56.
  18. Knych HK, Casbeer HC, McKemie DS, Arthur RM. Pharmacokinetics and pharmacodynamics of butorphanol following intravenous administration to the horse.. J Vet Pharmacol Ther 2012;36:21-30.
  19. Kalpravidh M, Lumb WV, Wright M, Heath RB. Analgesic effects of butorphanol in horses: dose-response studies.. Am J Vet Res 1984;45(2):211-216.
  20. Peters FT, Drummer OH, Musshoff F. Validation of new methods.. Forensic Sci Int 2007;165(2):216-224.
  21. . Agilent Mass Profiler Professional operation for chemometric analysis.. Vol. 1. USA: Agilent Technologies; 2019:1-310.
  22. Paine SW, Bright J, Scarth JP. The intravenous pharmacokinetics of butorphanol and detomidine dosed in combination compared with individual dose administrations to exercised horses.. J Vet Pharmacol Ther 2020;43(2):162-170.
  23. Sellon DC, Papich MG, Palmer L, Remund B. Pharmacokinetics of butorphanol in horses after intramuscular injection.. J Vet Pharmacol Ther 2009;32(1):62-65.
  24. Scarth JP, Teale P, Kuuranne T. Drug metabolism in the horse: a review.. Drug Test Anal 2011;3(1):19-53.

Citations

This article has been cited 1 times.
  1. Klingberg J, Keen B, Cawley A, Pasin D, Fu S. Developments in high-resolution mass spectrometric analyses of new psychoactive substances.. Arch Toxicol 2022 Apr;96(4):949-967.
    doi: 10.1007/s00204-022-03224-2pubmed: 35141767google scholar: lookup
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