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Home / Equine Research Bank / Molecules / Towards Non-Targeted Screening of Lipid Biomarkers for Impro...
Molecules (Basel, Switzerland)2022; 28(1); 312; doi: 10.3390/molecules28010312

Towards Non-Targeted Screening of Lipid Biomarkers for Improved Equine Anti-Doping.

Abstract: The current approach to equine anti-doping is focused on the targeted detection of prohibited substances. However, as new substances are rapidly being developed, the need for complimentary methods for monitoring is crucial to ensure the integrity of the racing industry is upheld. Lipidomics is a growing field involved in the characterisation of lipids, their function and metabolism in a biological system. Different lipids have various biological effects throughout the equine system including platelet aggregation and inflammation. A certain class of lipids that are being reviewed are the eicosanoids (inflammatory markers). The use of eicosanoids as a complementary method for monitoring has become increasingly popular with various studies completed to highlight their potential. Studies including various corticosteroids, non-steroidal anti-inflammatories and cannabidiol have been reviewed to highlight the progress lipidomics has had in contributing to the equine anti-doping industry. This review has explored the techniques used to prepare and analyse samples for lipidomic investigations in addition to the statistical analysis and potential for lipidomics to be used for a longitudinal assessment in the equine anti-doping industry.
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Publication Date: 2022-12-30 PubMed ID: 36615506PubMed Central: PMC9822433DOI: 10.3390/molecules28010312Google Scholar: Lookup
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  • Review

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research focuses on the potential integration and benefits of lipidomics in equine anti-doping methods. It specifically explores the use of inflammatory markers called eicosanoids as a monitoring approach, along with conventional methods for detecting illegal substances.

Research Background

  • The paper is established on the current framework of equine anti-doping, which primarily focuses on detecting illegal substances in horse racing.
  • Contrary to this targeted detection method, the research emphasizes the need for more comprehensive approaches as new racing enhancements are rapidly developed.

Lipidomics and its Relevance

  • Lipidomics, as presented in this study, is a growing area of research dedicated to identifying and understanding the roles and metabolic processes of lipids in biological systems.
  • As shared in this paper, different types of lipids serve varying biological functions throughout the horse’s system, including platelet aggregation and inflammation.
  • The paper particularly focuses on a group of lipids known as eicosanoids, which are markers of inflammation.
  • Using eicosanoids as a complementary method to traditional anti-doping measures has gained increasing attention, and the study reviews the several completed research highlighting their potential usefulness.

Eicosanoids and Existing Studies

  • Various studies conducted on corticosteroids, non-steroidal anti-inflammatories, and cannabidiol have been analyzed to determine the advancements lipidomics has contributed to detecting doping in horse racing.
  • These research works have shown noticeable progress in the application and accuracy of lipidomics in anti-doping mechanisms.

Methodologies in Lipidomic Investigations

  • The research further discusses the approaches used for sample preparation and analysis for lipidomic studies.
  • It also delves into the statistical analysis involved and the potential to use lipidomics for longitudinal assessments, thereby giving an extensive grasp of the techniques within the lemma of lipidomics.

Conclusion and Future Applications

  • The research underscores the importance and benefits of integrating lipidomics with current anti-doping strategies.
  • However, it also calls for further research to solidify the role of lipidomics, particularly the use of eicosanoids, in maintaining the integrity of horse racing.

Cite This Article

APA
Tou K, Cawley A, Bowen C, Bishop DP, Fu S. (2022). Towards Non-Targeted Screening of Lipid Biomarkers for Improved Equine Anti-Doping. Molecules, 28(1), 312. https://doi.org/10.3390/molecules28010312

Publication

Molecules (Basel, Switzerland)
ISSN: 1420-3049
NlmUniqueID: 100964009
Country: Switzerland
Language: English
Volume: 28
Issue: 1
PII: 312

Researcher Affiliations

Tou, Kathy
  • Centre for Forensic Science, University of Technology Sydney, Sydney, NSW 2007, Australia.
Cawley, Adam
  • Australian Racing Forensic Laboratory, Racing NSW, Sydney, NSW 2000, Australia.
Bowen, Christopher
  • Mass Spectrometry Business Unit, Shimadzu Scientific Instruments (Australasia), Sydney, NSW 2116, Australia.
Bishop, David P
  • Hyphenated Mass Spectrometry Laboratory (HyMAS), University of Technology, Sydney, NSW 2007, Australia.
Fu, Shanlin
  • Centre for Forensic Science, University of Technology Sydney, Sydney, NSW 2007, Australia.

MeSH Terms

  • Animals
  • Horses
  • Lipidomics
  • Inflammation
  • Lipids
  • Biomarkers
  • Eicosanoids
  • Lipid Metabolism

Conflict of Interest Statement

The authors declare no conflict of interest.

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