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Reproduction in domestic animals = Zuchthygiene2023; doi: 10.1111/rda.14462

Transcriptome analyses reveal transcriptional profiles of horse oocytes before and after in vitro maturation.

Abstract: Oocyte in vitro maturation is necessary for the study and application of animal-assisted reproduction technology in animal reproduction and breeding. The comprehensive transcriptional profile of equine oocyte maturated in vitro has not been fully mined yet, which makes many key transcriptional events still unidentified. Here, Smart-seq2 was performed to analyse the gene expression pattern and the underlying regulatory mechanism of horse germinal vesicle (GV) and in vitro metaphase II (MII) oocytes. The results showed that 6402 genes (2640 up-regulated and 3762 down-regulated in MII samples compared to GV) and 4021 lncRNA transcripts (1210 up-regulated and 2811 down-regulated in MII samples compared to GV) were differentially expressed in GV and MII oocytes. Further, GO and KEGG analysis found that differentially expressed mRNAs and lncRNAs were mainly enriched in the pathways related to energy and lipid metabolism. In addition, LGALS3 was found a key gene in mediating the regulation of oocyte meiosis recovery and fertilization ability. This study provides novel knowledge about gene expression and energy metabolism during equine oocyte maturation and a reference for the further study and application of assisted reproductive technology in horse reproduction and breeding.
© 2023 Wiley-VCH GmbH. Published by John Wiley & Sons Ltd.
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Publication Date: 2023-08-31 PubMed ID: 37650336DOI: 10.1111/rda.14462Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article provides insight into the gene expression and energy metabolism during equine oocyte maturation, which is integral for advancements in animal-assisted reproduction technology in horse breeding. In this study, the researchers investigated transcriptional changes in horse oocytes before and after in vitro maturation using Smart-seq2 analysis.

Objective and Methodology

  • The main goal of this research was to gain a comprehensive understanding of the gene expression patterns in horse oocytes during in vitro maturation. This would provide the researchers with an understanding of the important transcriptional events that take place during this crucial phase.
  • The researchers used a method known as Smart-seq2 to analyze the gene expression pattern in germinal vesicle (GV) and in vitro metaphase II (MII) oocytes. The GV stage refers to immature oocytes, while MII refers to mature in vitro oocytes.

Results and Findings

  • The data revealed that 6402 genes were differentially expressed in GV and MII oocytes. Of these, 2640 were up-regulated and 3762 were down-regulated in MII samples compared to GV.
  • Additionally, 4021 lncRNA (long noncoding RNA) transcripts were also differentially expressed in GV and MII oocytes. Here, 1210 were up-regulated and 2811 were down-regulated in MII samples compared to GV.
  • Following GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis, it was determined that these differentially expressed mRNAs and lncRNAs are predominantly involved in pathways related to energy and lipid metabolism. This suggests that these processes likely play a significant role during oocyte maturation.
  • A gene called LGALS3 was identified as a means of potentially directing the regulation of oocyte meiosis recovery and fertilization ability.

Conclusion and Relevance

  • This research is a pioneering effort to understand the intricate process of equine oocyte maturation in the context of gene expression and energy metabolism.
  • The findings offer essential insights for animal-assistant reproduction technology, particularly in horse reproduction and breeding.
  • The study identified key transcriptional events and deepens the understanding of energy and lipid metabolism pathways in oocyte maturation.
  • The outcome of the research can provide references for further study and applications in the field, especially those related to enhancing the effectiveness of assisted reproductive strategies in horse breeding.

Cite This Article

APA
Song X, Jiang H, Lv P, Cui K, Liu Q, Yin S, Liu H, Li Z. (2023). Transcriptome analyses reveal transcriptional profiles of horse oocytes before and after in vitro maturation. Reprod Domest Anim. https://doi.org/10.1111/rda.14462

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English

Researcher Affiliations

Song, Xinhui
  • State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning, China.
Jiang, Hancai
  • State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning, China.
Lv, Peiru
  • Henan Chuangyuan Biotechnology Co. Ltd, Zhengzhou, China.
Cui, Kuiqing
  • State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning, China.
  • Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan, China.
Liu, Qingyou
  • State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning, China.
  • Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan, China.
Yin, Shan
  • Henan Chuangyuan Biotechnology Co. Ltd, Zhengzhou, China.
Liu, Hongbo
  • Henan Chuangyuan Biotechnology Co. Ltd, Zhengzhou, China.
Li, Zhipeng
  • State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning, China.

Grant Funding

  • National Natural Science Foundation Project
  • Science and Technology Major Project of Guangxi
  • the Key Research and Development Project of Qingxiu District of Nanning City

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Citations

This article has been cited 1 times.
  1. Cui J, Zhao X, Hao J, Liu X, Wang W, He L, Wang Y, Rong J, Qiu C, Chen D, Cheng L, Tian J, Zhang J, Xi G. Cross-Species Insights into In Vitro Maturation Defects of the Oocyte and Identification of Crucial Regulators for Sheep Oocyte Maturation. Antioxidants (Basel) 2025 Dec 13;14(12).
    doi: 10.3390/antiox14121499pubmed: 41462698google scholar: lookup
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