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International journal of molecular sciences2019; 20(21); 5474; doi: 10.3390/ijms20215474

Transforming Growth Factor Beta 3-Loaded Decellularized Equine Tendon Matrix for Orthopedic Tissue Engineering.

Abstract: Transforming growth factor beta 3 (TGFβ3) promotes tenogenic differentiation and may enhance tendon regeneration in vivo. This study aimed to apply TGFβ3 absorbed in decellularized equine superficial digital flexor tendon scaffolds, and to investigate the bioactivity of scaffold-associated TGFβ3 in an in vitro model. TGFβ3 could effectively be loaded onto tendon scaffolds so that at least 88% of the applied TGFβ3 were not detected in the rinsing fluid of the TGFβ3-loaded scaffolds. Equine adipose tissue-derived multipotent mesenchymal stromal cells (MSC) were then seeded on scaffolds loaded with 300 ng TGFβ3 to assess its bioactivity. Both scaffold-associated TGFβ3 and TGFβ3 dissolved in the cell culture medium, the latter serving as control group, promoted elongation of cell shapes and scaffold contraction ( < 0.05). Furthermore, scaffold-associated and dissolved TGFβ3 affected MSC musculoskeletal gene expression in a similar manner, with an upregulation of tenascin c and downregulation of other matrix molecules, most markedly decorin ( < 0.05). These results demonstrate that the bioactivity of scaffold-associated TGFβ3 is preserved, thus TGFβ3 application via absorption in decellularized tendon scaffolds is a feasible approach.
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Publication Date: 2019-11-03 PubMed ID: 31684150PubMed Central: PMC6862173DOI: 10.3390/ijms20215474Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the application of Transforming Growth Factor Beta 3 (TGFβ3) into a decellularized equine tendon scaffold, to stimulate tenogenic differentiation which could enhance tendon regeneration in vivo. The study confirms that the bioactivity of this growth factor is maintained, providing a promising approach for orthopedic tissue engineering.

Objective and Methodology

  • The aim of the study was to use TGFβ3, absorbed in decellularized scaffolds from equine superficial digital flexor tendons, and investigate its bioactivity in an in vitro model.
  • TGFβ3 is known to promote tenogenic differentiation, which enhances tendon regeneration in vivo. The scaffolds provided a platform to verify if the bioactivity of TGFβ3 is maintained in such settings.
  • A significant portion, at least 88%, of TGFβ3 applied could be effectively loaded onto tendon scaffolds and was not detected in the rinsing fluid of these scaffolds, affirming effective absorption.

Findings

  • The researchers tested the bioactivity of TGFβ3 by seeding equine adipose tissue-derived multipotent mesenchymal stromal cells (MSC) on scaffolds loaded with 300 ng TGFβ3.
  • Observations were that both scaffold-associated TGFβ3 and TGFβ3 dissolved in the cell culture medium promoted cell elongation and scaffold contraction.
  • Examination of gene expression showed that both scaffold-associated and dissolved TGFβ3 affected MSC musculoskeletal gene expression, triggering an upregulation of tenascin c and downregulation of other matrix molecules, particularly decorin.

Implications

  • These results demonstrate that the bioactivity of scaffold-associated TGFβ3 is preserved, pointing to a feasible method for TGFβ3 application.
  • This finding is valuable in the context of orthopedic tissue engineering, as it could be a significant approach for tendon regeneration.
  • Furthermore, the study presents potential opportunities for developing more advanced treatment styles based on the application of bioactive growth factor to tendon matrices.

Cite This Article

APA
Roth SP, Brehm W, Groß C, Scheibe P, Schubert S, Burk J. (2019). Transforming Growth Factor Beta 3-Loaded Decellularized Equine Tendon Matrix for Orthopedic Tissue Engineering. Int J Mol Sci, 20(21), 5474. https://doi.org/10.3390/ijms20215474

Publication

International journal of molecular sciences
ISSN: 1422-0067
NlmUniqueID: 101092791
Country: Switzerland
Language: English
Volume: 20
Issue: 21
PII: 5474

Researcher Affiliations

Roth, Susanne Pauline
  • Faculty of Veterinary Medicine, Veterinary Teaching Hospital, Department for Horses, University of Leipzig, D 04103 Leipzig, Germany. Susanne.roth@uni-leipzig.de.
  • Saxonian Incubator for Clinical Translation, University of Leipzig, D-04103 Leipzig, Germany. Susanne.roth@uni-leipzig.de.
Brehm, Walter
  • Faculty of Veterinary Medicine, Veterinary Teaching Hospital, Department for Horses, University of Leipzig, D 04103 Leipzig, Germany. brehm@vetmed.uni-leipzig.de.
Groß, Claudia
  • Saxonian Incubator for Clinical Translation, University of Leipzig, D-04103 Leipzig, Germany. claudia.gross@sikt.uni-leipzig.de.
Scheibe, Patrick
  • Saxonian Incubator for Clinical Translation, University of Leipzig, D-04103 Leipzig, Germany. pscheibe@sikt.uni-leipzig.de.
Schubert, Susanna
  • Saxonian Incubator for Clinical Translation, University of Leipzig, D-04103 Leipzig, Germany. susanna.schubert@sikt.uni-leipzig.de.
Burk, Janina
  • Saxonian Incubator for Clinical Translation, University of Leipzig, D-04103 Leipzig, Germany. Janina.Burk@vetmed.uni-giessen.de.
  • Faculty of Veterinary Medicine, Equine Clinic-Surgery, Justus-Liebig-University Giessen, D-35392 Giessen, Germany. Janina.Burk@vetmed.uni-giessen.de.

MeSH Terms

  • Animals
  • Cell Differentiation
  • Cells, Cultured
  • Decorin / genetics
  • Decorin / metabolism
  • Extracellular Matrix / metabolism
  • Gene Expression Regulation
  • Horses
  • Humans
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism
  • Musculoskeletal System / metabolism
  • Tenascin / genetics
  • Tenascin / metabolism
  • Tendons / cytology
  • Tendons / physiology
  • Tissue Engineering / methods
  • Tissue Scaffolds
  • Transforming Growth Factor beta3 / metabolism

Grant Funding

  • BMBF 1315883 / Bundesministerium fu00fcr Bildung und Forschung
  • DFG BU3110/1-1 / Deutsche Forschungsgemeinschaft

Conflict of Interest Statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

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