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Home / Equine Research Bank / Immunology / Transforming growth factor-beta induced by live or ultraviol...
Immunology1997; 90(4); 586-591; doi: 10.1046/j.1365-2567.1997.00202.x

Transforming growth factor-beta induced by live or ultraviolet-inactivated equid herpes virus type-1 mediates immunosuppression in the horse.

Abstract: Up to 21 days after exposure to live or ultraviolet-inactivated equid herpesvirus type-1 (EHV-1) autologous serum from ponies caused an immunosuppressive effect if incorporated into T-cell proliferation assays to EHV-1. The suppressive factor in the sera of ponies also inhibited T-cell response to phytohaemagglutinin. Increased levels of circulating activated transforming growth factor-beta 1 (TGF-beta 1) were detected, and the suppressive activity of the serum could be reversed by antibody to TGF-beta 1. In a challenge experiment the ponies which exhibited circulating TGF-beta 1 activity succumbed to infection while the ones with similar magnitudes of T-cell responses, but no TGF-beta 1 activity, were protected. A definition of this immunosuppressive mechanism and its mode of induction must be central to the design of vaccines and to an understanding of the pathogenesis of EHV-1.
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Publication Date: 1997-04-01 PubMed ID: 9176113PubMed Central: PMC1456683DOI: 10.1046/j.1365-2567.1997.00202.xGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a study on the immunosuppressive effects of equid herpes virus type-1 (EHV-1) on horses, mediated by increased levels of the transforming growth factor-beta 1 (TGF-beta 1).

Research Context and Methodology

  • The study involves the equid herpes virus type-1 (EHV-1), a virus that can cause a variety of diseases in horses, including neurological disorders, respiratory diseases, and abortions.
  • The researchers exposed horses to live or ultraviolet-inactivated EHV-1 for up to 21 days. They then integrated the horses’ serum into T-cell proliferation assays to assess the effects on EHV-1.
  • The researchers also investigated the effect of the serum on T-cell response to phytohaemagglutinin, a substance known to stimulate T-cell proliferation.

Key Findings

  • The serum from the exposed horses caused suppression of the T-cell responses, hence an immunosuppressive effect. This was also evident in the response to phytohaemagglutinin, showing the general inhibitory impact on T-cell proliferation.
  • Increased levels of the transforming growth factor-beta 1 (TGF-beta 1) were observed upon exposure to the virus. TGF-beta 1 is a protein involved in cell differentiation and immune regulation, among other physiological processes.
  • This suppressive effect of the serum could be turned around by using an antibody to TGF-beta 1, indicating a significant role of TGF-beta 1 in mediating the immunosuppressive effects of EHV-1.
  • In a subsequent experiment, horses showing circulating TGF-beta 1 activity were found to succumb to infection, whereas those with similar magnitudes of T-cell responses but without TGF-beta 1 activity were protected against infection.

Conclusions and Implications

  • The research establishes the key role of TGF-beta 1 in mediating the immunosuppressive effects of the EHV-1 virus in horses. This discovery has significant implications for the understanding of EHV-1 pathogenesis.
  • Understanding this immunosuppressive mechanism could be central to designing vaccines against EHV-1. By targeting TGF-beta 1, it might be possible to reverse the inhibitory effects on T-cell proliferation and hence, enhance immune responses against the virus.

Cite This Article

APA
Charan S, Palmer K, Chester P, Mire-Sluis AR, Meager A, Edington N. (1997). Transforming growth factor-beta induced by live or ultraviolet-inactivated equid herpes virus type-1 mediates immunosuppression in the horse. Immunology, 90(4), 586-591. https://doi.org/10.1046/j.1365-2567.1997.00202.x

Publication

Immunology
ISSN: 0019-2805
NlmUniqueID: 0374672
Country: England
Language: English
Volume: 90
Issue: 4
Pages: 586-591

Researcher Affiliations

Charan, S
  • Department of Pathology & Infectious Diseases, Royal Veterinary College, London, UK.
Palmer, K
    Chester, P
      Mire-Sluis, A R
        Meager, A
          Edington, N

            MeSH Terms

            • Animals
            • Herpesviridae Infections / immunology
            • Herpesviridae Infections / veterinary
            • Herpesvirus 1, Equid / immunology
            • Herpesvirus 1, Equid / radiation effects
            • Horse Diseases / immunology
            • Horses
            • Immune Tolerance
            • Lymphocyte Culture Test, Mixed
            • Polymerase Chain Reaction
            • Transforming Growth Factor beta / immunology
            • Ultraviolet Rays

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            Citations

            This article has been cited 5 times.
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