Treatment of a bone defect consequent to the removal of a periapical cyst with equine bone and equine membranes: clinical and histological outcome.
Abstract: While deproteinized bovine bone and bovine membranes have been well studied and can yield good results when used to treat bone defects and peri-implant dehiscences, enzymatically deantigenated equine bone and equine membranes have emerged as possible alternative biomaterials. The objective of this study was the clinical and histological assessment of such materials: equine bone granules, an equine collagen membrane and an equine pericardium membrane. Methods: Enzymatically deantigenated equine bone and an equine collagen membrane were used to restore a bone defect caused by the removal of a bone cyst in the upper anterior maxilla. After 4.5 months, an implant was placed and a bone core sample was obtained from the grafted site. Implants threads, though, were exposed. This defect was grafted with a mixture of autogenous and equine bone and covered with an equine pericardium membrane. Results: Four months after implant placement the peri-implant bone levels were maintained. A prosthesis was delivered three months later providing functional and esthetic rehabilitation. Also four-year follow-up controls showed implant success. Histological analysis of the bone core revealed that the graft material had undergone remodelling, and a fair amount of newly formed vital bone was present at the time of sample collection. Conclusions: The deantigenated equine bone is biocompatible and undergoes osteoclastic remodelling. Both the equine collagen and pericardium membrane acted as effective barriers for guided bone regeneration.
Publication Date: 2012-12-05 PubMed ID: 23207673
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Case Reports
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This article describes a study investigating the use of equine bone and membranes to treat bone defects. The researchers found that these materials, after being treated to remove antigens, are effective and safe for use in bone regeneration and dental implants.
Background
- The paper focuses on the use of deproteinized bovine bone and membranes to treat bone defects and peri-implant dehiscences, with an emphasis on the newly examined alternatives – equine bone and membranes.
- Enzymatically deantigenated equine bone and membranes are the newly proposed biomaterials substituting for the bovine equivalents.
- The primary objective of the study was to clinically and histologically assess the potential of these equine materials when treating bone defects, focusing on equine bone granules, an equine collagen membrane, and an equine pericardium membrane.
Research Methodology
- The study used enzyme-treated equine bone and an equine collagen membrane to restore a bone defect caused by the removal of a bone cyst in the upper anterior maxilla, a portion of the human jaw.
- After 4.5 months, a dental implant was placed at the site, and a sample was collected from the grafted bone for further analysis. Minor imperfections were observed with the implant positioning, with some parts of the implant threads being exposed.
- This minor defect was addressed by grafting a mixture of patient’s own bone (autogenous bone) and equine bone, covered with an equine pericardium membrane.
Results
- After another four months, the peri-implant bone levels were maintained, and a functional prosthetic was delivered three months later, providing both aesthetic and functional rehabilitation.
- The study conducted follow-ups over a period of four years after treatment, during which the implant success was observed.
- A histological (microscopic) analysis of the bone sample showed that the equine bone graft material had undergone remodeling, and a significant amount of new, vital bone growth was seen in the sample.
Conclusion
- The study concluded that the antigen-removed equine bone is compatible with human biology and undergoes normal bone remodeling processes. Both the equine collagen and pericardium membrane were successful in acting as barriers for guided bone regeneration.
Cite This Article
APA
Di Stefano DA, Andreasi Bassi M, Cinci L, Pieri L, Ammirabile G.
(2012).
Treatment of a bone defect consequent to the removal of a periapical cyst with equine bone and equine membranes: clinical and histological outcome.
Minerva Stomatol, 61(11-12), 477-490.
Publication
Researcher Affiliations
- Department of Dentistry, Vita e Salute San Raffaele University, Milan, Italy. distefano@centrocivitali.it
MeSH Terms
- Animals
- Bone Transplantation
- Female
- Horses
- Humans
- Membranes / transplantation
- Middle Aged
- Radicular Cyst / surgery
- Treatment Outcome
Citations
This article has been cited 6 times.- Di Stefano DA, Orlando F, Ottobelli M, Fiori D, Garagiola U. A comparison between anorganic bone and collagen-preserving bone xenografts for alveolar ridge preservation: systematic review and future perspectives.. Maxillofac Plast Reconstr Surg 2022 Jul 12;44(1):24.
- Gallo N, Natali ML, Sannino A, Salvatore L. An Overview of the Use of Equine Collagen as Emerging Material for Biomedical Applications.. J Funct Biomater 2020 Nov 1;11(4).
- Di Stefano DA, Zaniol T, Cinci L, Pieri L. Chemical, Clinical and Histomorphometric Comparison between Equine Bone Manufactured through Enzymatic Antigen-Elimination and Bovine Bone Made Non-Antigenic Using a High-Temperature Process in Post-Extractive Socket Grafting. A Comparative Retrospective Clinical Study.. Dent J (Basel) 2019 Jul 1;7(3).
- Droutsas K, Papaconstantinou D, Georgalas I, Kymionis G, Andreanos K, Stamou E, Mamas N. Equine pericardium for tectonic globe repair.. Int Ophthalmol 2019 Jan;39(1):225-230.
- Centonze R, Agostini E, Massaccesi S, Toninelli S, Morabito L. A novel equine-derived pericardium membrane for dural repair: A preliminary, short-term investigation.. Asian J Neurosurg 2016 Jul-Sep;11(3):201-5.
- Di Stefano DA, Greco GB, Riboli F. Guided Bone Regeneration of an Atrophic Mandible with a Heterologous Bone Block.. Craniomaxillofac Trauma Reconstr 2016 Mar;9(1):88-93.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
