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Frontiers in veterinary science2018; 5; 40; doi: 10.3389/fvets.2018.00040

Trypanosoma equiperdum Low Molecular Weight Proteins As Candidates for Specific Serological Diagnosis of Dourine.

Abstract: The diagnosis of dourine can be difficult because the clinical signs of this disease in horses are similar to those of surra, caused by . Moreover, and are closely related and, so far, they cannot be distinguished using serological tests. In a previous work, the protein pattern recognized by antibodies from dourine-infected horses and the humoral immune response kinetics were investigated by immunoblotting assay; a total of 20 sera from naturally and experimentally infected horses and from healthy animals were tested. Immunoblotting analysis showed that antibodies from infected horses specifically bind low molecular weight proteins (from 16 to 35 kDa), which are not recognized by antibodies from uninfected horses. In this work, we tested other 615 sera (7 from naturally infected horses and 608 sera from healthy horses and donkeys): results confirmed the data obtained previously. In addition, six SDS-PAGE bands with molecular weight ranging from 10 to 37 kDa were analyzed by mass spectrometry, in order to identify immunogenic proteins that could be used as biomarkers for the diagnosis of dourine. A total of 167 proteins were identified. Among them, 37 were found unique for . Twenty-four of them could represent possible candidate diagnostic antigens for the development of serological tests specific for .
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Publication Date: 2018-03-05 PubMed ID: 29556505PubMed Central: PMC5844913DOI: 10.3389/fvets.2018.00040Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study focuses on identifying unique proteins in Trypanosoma equiperdum, the pathogen causing dourine disease in horses, for more effective diagnostic tests. Sorting through multiple protein identifiers, 24 proteins were uncovered as potential exclusive markers for dourine, possibly aiding the development of more disease-specific serological tests.

Understanding Dourine Diagnosis Difficulties

  • Dourine, a disease in horses caused by Trypanosoma equiperdum, shares clinical signs with surra, a disease caused by different Trypanosoma species.
  • This similarity makes the diagnosis of dourine challenging as the two diseases cannot be distinguished using standard serological tests.
  • Trypanosoma species responsible for dourine and surra are closely related, adding to the difficulty in differential diagnosis.

Identifying Unique Proteins for Dourine Diagnosis

  • The researchers tested antibodies from both dourine-infected and healthy horses. The antibodies from infected horses specifically bonded with low molecular weight proteins of Trypanosoma equiperdum, not found in uninfected horses.
  • They further tested 615 sera from naturally infected and healthy horses and donkeys. The results confirmed the specificity of these proteins for Trypanosoma equiperdum.
  • Six distinct SDS-PAGE bands with molecular weight ranging from 10 to 37 kDa were examined via mass spectrometry to identify the immunogenic proteins serving as biomarkers for dourine.

Recognizing Diagnostic Antigens

  • From the mass spectrometry analysis, a total of 167 proteins were identified.
  • Among these, 37 proteins were unique to Trypanosoma equiperdum.
  • Of these 37 unique proteins, 24 were determined to be potential diagnostic antigens for dourine.
  • These proteins could potentially be used to develop more specific serological tests for diagnosing dourine, improving differentiation from other similar diseases, like surra.

Cite This Article

APA
Luciani M, Di Febo T, Orsini M, Krasteva I, Cattaneo A, Podaliri Vulpiani M, Di Pancrazio C, Bachi A, Tittarelli M. (2018). Trypanosoma equiperdum Low Molecular Weight Proteins As Candidates for Specific Serological Diagnosis of Dourine. Front Vet Sci, 5, 40. https://doi.org/10.3389/fvets.2018.00040

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 5
Pages: 40
PII: 40

Researcher Affiliations

Luciani, Mirella
  • Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale, Teramo, Italy.
Di Febo, Tiziana
  • Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale, Teramo, Italy.
Orsini, Massimiliano
  • Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale, Teramo, Italy.
Krasteva, Ivanka
  • Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale, Teramo, Italy.
Cattaneo, Angela
  • Istituto FIRC di Oncologia Molecolare (IFOM), Milan, Italy.
Podaliri Vulpiani, Michele
  • Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale, Teramo, Italy.
Di Pancrazio, Chiara
  • Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale, Teramo, Italy.
Bachi, Angela
  • Istituto FIRC di Oncologia Molecolare (IFOM), Milan, Italy.
Tittarelli, Manuela
  • Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale, Teramo, Italy.

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Citations

This article has been cited 5 times.
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