Tumor necrosis factor activity in serum from neonatal foals with presumed septicemia.
Abstract: A study was performed to determine prevalence of tumor necrosis factor (TNF) activity in serum of equine neonates with presumed sepsis and to determine correlation between serum TNF activity and severity and outcome of disease. Twenty foals less than 21 days old were considered suitable for inclusion in this study by satisfying clinical and laboratory criteria suggestive of septicemia. At admission, blood samples were collected from all foals for determination of serum TNF activity, then clinical course and outcome of disease were recorded. Thirty-one clinically normal foals less than 21 days old served as controls for serum TNF activity. Serum TNF activity was estimated by use of an in vitro cytotoxicity bioassay and WEHI 164 clone-13 murine fibrosarcoma cells. Of the 20 foals with presumed sepsis, 5 had high serum TNF activity. Mean heart rate (P less than 0.005), mucosal petechial hemorrhages (P = 0.06), and death rate (P = 0.06) were greater in the group of foals with high serum TNF activity. These foals also had a lower mean neutrophil count (P less than 0.001), greater band-to-segmented neutrophil ratio (P less than 0.0001), and more prevalent neutrophil toxic changes (P = 0.07) than did foals without serum TNF activity (P = 0.02). Joint swelling was more prevalent in foals without serum TNF activity. Results of the study indicate that serum TNF activity is correlated with clinical criteria of sepsis in equine neonates. An association was apparent between disease severity and serum TNF activity in this group of foals with presumed septicemia.
Publication Date: 1991-12-01 PubMed ID: 1778741
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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This research article presents a study conducted on neonatal foals suspected of having sepsis to assess the prevalence of tumor necrosis factor (TNF) activity in their serum, and how this activity is potentially correlated with the severity and outcome of their condition.
Research Objective and Methodology
- The research aimed to understand the role of TNF activity in serum of young foals (less than 21 days old) thought to be suffering from septicemia.
- The study involved 20 foals who met the criteria for potential septicemia based on clinical and laboratory results.
- For comparison, 31 healthy foals of the same age were used as controls.
- Blood samples were taken from all foals at admission to measure serum TNF activity.
- This TNF activity was gauged through an in vitro cytotoxicity bioassay and WEHI 164 clone-13 murine fibrosarcoma cells.
- As the subjects were monitored, the clinical course and outcomes of the disease were documented.
Finding and Implication
- The study found that 5 out of the 20 foals with suspected sepsis had high serum TNF activity.
- A comparison between the group of foals with high serum TNF activity and the control group showed significant differences in heart rate, mucosal petechial hemorrhages, death rate, neutrophil count, neutrophil ratio, and neutrophil toxic changes.
- These findings suggested that high serum TNF activity might be associated with severe manifestations of the disease, especially as it related to heart rate, hemorrhages, and neutrophil abnormalities.
- Surprisingly, joint swelling was seen more in foals without serum TNF activity.
- The study concluded that serum TNF activity correlates with clinical signs of sepsis in equine neonates and that its presence could potentially indicate a more severe prognosis.
Importance of the Research
- This research not only helped in a better understanding of equine neonatal sepsis but also presented TNF as potential marker for severity of the disease.
- By better understanding the roles and effects of TNF in the serum, scientists can explore targeted treatments and interventions for septicemia.
Cite This Article
APA
Morris DD, Moore JN.
(1991).
Tumor necrosis factor activity in serum from neonatal foals with presumed septicemia.
J Am Vet Med Assoc, 199(11), 1584-1589.
Publication
Researcher Affiliations
- Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602.
MeSH Terms
- Animals
- Animals, Newborn
- Bacteremia / blood
- Bacteremia / diagnosis
- Bacteremia / veterinary
- Heart Rate
- Horse Diseases / blood
- Horse Diseases / diagnosis
- Horses
- Leukocyte Count / veterinary
- Neutrophils
- Prognosis
- Tumor Necrosis Factor-alpha / analysis
Citations
This article has been cited 5 times.- Sheats MK. A Comparative Review of Equine SIRS, Sepsis, and Neutrophils. Front Vet Sci 2019;6:69.
- Rütten S, Schusser GF, Abraham G, Schrödl W. Release kinetics of tumor necrosis factor-α and interleukin-1 receptor antagonist in the equine whole blood. BMC Vet Res 2016 Jun 17;12(1):117.
- Hart KA, Barton MH, Vandenplas ML, Hurley DJ. Effects of low-dose hydrocortisone therapy on immune function in neonatal horses. Pediatr Res 2011 Jul;70(1):72-7.
- Morris DD, Crowe N, Moore JN. Reduced endotoxin-induced production of tumor necrosis factor activity by equine peritoneal macrophages exposed to the dual inhibitor of arachidonic acid metabolism, SK & F 86002. Can J Vet Res 1992 Apr;56(2):110-4.
- Hajimohammadi A, Ghane M, Ghari Tehrani M, Paravar B, Mirzaei A, Razavi S, Nikzad M. Association of the severity of colic in horses with oxidative stress biomarkers, acute-phase proteins, and certain trace elements. J Equine Sci 2023 Sep;34(3):73-81.
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