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Home / Equine Research Bank / Oecologia / Turnover of carbon isotopes in tail hair and breath CO2 of h...
Oecologia2004; 139(1); 11-22; doi: 10.1007/s00442-003-1479-x

Turnover of carbon isotopes in tail hair and breath CO2 of horses fed an isotopically varied diet.

Abstract: Temporal stable isotope records derived from animal tissues are increasingly studied to determine dietary and climatic histories. Despite this, the turnover times governing rates of isotope equilibration in specific tissues following a dietary isotope change are poorly known. The dietary isotope changes recorded in the hair and blood bicarbonate of two adult horses in this study are found to be successfully described by a model having three exponential isotope pools. For horse tail hair, the carbon isotope response observed following a dietary change from a C3 to a C4 grass was consistent with a pool having a very fast turnover rate ( t1/2 approximately 0.5 days) that made up approximately 41% of the isotope signal, a pool with an intermediate turnover rate ( t1/2 approximately 4 days) that comprised approximately 15% of the isotope signal, and a pool with very slow turnover rate ( t1/2 approximately 140 days) that made up approximately 44% of the total isotope signal. The carbon isotope signature of horse blood bicarbonate, in contrast, had a different isotopic composition, with approximately 67% of the isotope signal coming from a fast turnover pool ( t1/2 0.2 days), approximately 17% from a pool with an intermediate turnover rate ( t1/2 approximately 3 days) and approximately 16% from a pool with a slow turnover rate ( t1/2 approximately 50 days). The constituent isotope pools probably correspond to one exogenous and two endogenous sources. The exogenous source equates to our fast turnover pool, and the pools with intermediate and slow turnover rates are thought to derive from the turnover of metabolically active tissues and relatively inactive tissues within the body, respectively. It seems that a greater proportion of the amino acids available for hair synthesis come from endogenous sources compared to the compounds undergoing cellular catabolism in the body. Consequently, the isotope composition of blood bicarbonate appears to be much more responsive to dietary isotope changes, whereas the amino acids in the blood exhibit considerable isotopic inertia.
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Publication Date: 2004-01-17 PubMed ID: 14730442DOI: 10.1007/s00442-003-1479-xGoogle Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores the rate of carbon isotope turnover in the tail hair and breath CO2 of horses subject to dietary changes. It proposes a three-pool conceptual model to characterize the observed isotopic response and offers insights into the contributing sources of carbon isotopes in horses.

Approach

  • The researchers examined isotope changes in two key tissues of two adult horses: tail hair and blood bicarbonate.
  • Horses’ diets were altered from a C3 grass (a plant that uses the C3 carbon fixing pathway) to a C4 grass (a plant using the C4 pathway) to track the isotopic shift.
  • The isotopic response was studied using a model that assumes three distinct pools of isotopes, each having different turnover rates.

Findings

  • For tail hair, the carbon isotope response post-dietary change was best described by a pool with a quick turnover rate (half-life approximately 0.5 days) which comprised about 41% of the isotope signal, a pool with an intermediate turnover rate (half-life about 4 days) contributing about 15%, and a pool with a slow turnover rate (half-life roughly 140 days) accounting for around 44% of the isotope signal.
  • The carbon isotope signature of horse blood bicarbonate was found to be composed of approximately 67% of the fast turnover pool (half-life 0.2 days), about 17% from an intermediate turnover rate pool (half-life around 3 days), and roughly 16% from a slow turnover rate pool (half-life approximately 50 days).

Interpretations

  • The researchers suggest that the pool with a quick turnover rate corresponds to an exogenous source, most probably the recent intake from diet.
  • The pools with intermediate and slow turnover rates are thought to be derived from the metabolic turnover of active and less active bodily tissues, respectively.
  • The study reveals that, in comparison to compounds undergoing cellular catabolism, more amino acids available for hair synthesis come from endogenous sources.
  • Therefore, it is easier for the isotopic composition of blood bicarbonate to respond to dietary changes, while blood amino acids maintain a stable isotopic makeup.

Cite This Article

APA
Ayliffe LK, Cerling TE, Robinson T, West AG, Sponheimer M, Passey BH, Hammer J, Roeder B, Dearing MD, Ehleringer JR. (2004). Turnover of carbon isotopes in tail hair and breath CO2 of horses fed an isotopically varied diet. Oecologia, 139(1), 11-22. https://doi.org/10.1007/s00442-003-1479-x

Publication

Oecologia
ISSN: 0029-8549
NlmUniqueID: 0150372
Country: Germany
Language: English
Volume: 139
Issue: 1
Pages: 11-22

Researcher Affiliations

Ayliffe, L K
  • Department of Geology and Geophysics, University of Utah, Salt Lake City, UT 84112, USA.
Cerling, T E
    Robinson, T
      West, A G
        Sponheimer, M
          Passey, B H
            Hammer, J
              Roeder, B
                Dearing, M D
                  Ehleringer, J R

                    MeSH Terms

                    • Amino Acids / blood
                    • Animal Feed
                    • Animals
                    • Breath Tests
                    • Carbon / metabolism
                    • Carbon Dioxide / analysis
                    • Carbon Isotopes / analysis
                    • Female
                    • Hair / chemistry
                    • Horses / physiology
                    • Male
                    • Models, Theoretical

                    References

                    This article includes 6 references
                    1. White CD, Schwarcz HP. Temporal trends in stable isotopes for Nubian mummy tissues.. Am J Phys Anthropol 1994 Feb;93(2):165-87.
                      pubmed: 8147434doi: 10.1002/ajpa.1330930203google scholar: lookup
                    2. Tieszen LL, Boutton TW, Tesdahl KG, Slade NA. Fractionation and turnover of stable carbon isotopes in animal tissues: Implications for δ(13)C analysis of diet.. Oecologia 1983 Mar;57(1-2):32-37.
                      pubmed: 28310153doi: 10.1007/BF00379558google scholar: lookup
                    3. Cerling TE, Harris JM. Carbon isotope fractionation between diet and bioapatite in ungulate mammals and implications for ecological and paleoecological studies.. Oecologia 1999 Aug;120(3):347-363.
                      pubmed: 28308012doi: 10.1007/s004420050868google scholar: lookup
                    4. Witt GB, MOLL EJ, BEETON RJS, Murray PJ. Isotopes, Wool, and Rangeland Monitoring: Let the Sheep Do the Sampling.. Environ Manage 1998 Jan;22(1):145-52.
                      pubmed: 9419292doi: 10.1007/s002679900091google scholar: lookup
                    5. O'Connell TC, Hedges RE. Investigations into the effect of diet on modern human hair isotopic values.. Am J Phys Anthropol 1999 Apr;108(4):409-25.
                      pubmed: 10229386doi: 10.1002/(SICI)1096-8644(199904)108:4<409::AID-AJPA3>3.0.CO;2-Egoogle scholar: lookup
                    6. DeNiro MJ, Epstein S. Mechanism of carbon isotope fractionation associated with lipid synthesis.. Science 1977 Jul 15;197(4300):261-3.
                      pubmed: 327543doi: 10.1126/science.327543google scholar: lookup

                    Citations

                    This article has been cited 54 times.
                    1. Wang HL, Zhao Y, Wang FJ, Sun XJ, Zhu JQ, Zhang YM, Wei SD, Chen H. Diet composition and selection of Père David's deer in Hubei Shishou Milu National Nature Reserve, China. Ecol Evol 2023 Jan;13(1):e9702.
                      doi: 10.1002/ece3.9702pubmed: 36620412google scholar: lookup
                    2. Viciano J, López-Lázaro S, Tanga C. Post-Mortem Dental Profile as a Powerful Tool in Animal Forensic Investigations-A Review. Animals (Basel) 2022 Aug 10;12(16).
                      doi: 10.3390/ani12162038pubmed: 36009628google scholar: lookup
                    3. Gelippi M, Caraveo-Patiño J, Gauger MFW, Popp BN, Panigada S, Marcín-Medina R. Isotopic composition of the eastern gray whale epidermis indicates contribution of prey outside Arctic feeding grounds. Sci Rep 2022 Apr 29;12(1):7055.
                      doi: 10.1038/s41598-022-10780-1pubmed: 35488113google scholar: lookup
                    4. Chidimuro B, Mundorff A, Speller C, Radini A, Boudreault N, Lucas M, Holst M, Lamb A, Collins M, Alexander M. Isotope analysis of human dental calculus δ(13) CO(3) (2-) : Investigating a potential new proxy for sugar consumption. Rapid Commun Mass Spectrom 2022 Jun 15;36(11):e9286.
                      doi: 10.1002/rcm.9286pubmed: 35261104google scholar: lookup
                    5. Mancuso CJ, Cornwall CM, Robinson S, Valenzuela LO, Ehleringer JR. Breath Stable Isotope Analysis Serves as a Non-invasive Analytical Tool to Demonstrate Dietary Changes in Adolescent Students Over Time. Front Med (Lausanne) 2021;8:697557.
                      doi: 10.3389/fmed.2021.697557pubmed: 35145970google scholar: lookup
                    6. Careddu G, Ciucci P, Mondovì S, Calizza E, Rossi L, Costantini ML. Gaining insight into the assimilated diet of small bear populations by stable isotope analysis. Sci Rep 2021 Jul 8;11(1):14118.
                      doi: 10.1038/s41598-021-93507-ypubmed: 34238974google scholar: lookup
                    7. Gelippi M, Popp B, Gauger MFW, Caraveo-Patiño J. Tracing gestation and lactation in free ranging gray whales using the stable isotopic composition of epidermis layers. PLoS One 2020;15(10):e0240171.
                      doi: 10.1371/journal.pone.0240171pubmed: 33119639google scholar: lookup
                    8. Chandrajith R, Weerasingha A, Premaratne KM, Gamage D, Abeygunasekera AM, Joachimski MM, Senaratne A. Mineralogical, compositional and isotope characterization of human kidney stones (urolithiasis) in a Sri Lankan population. Environ Geochem Health 2019 Oct;41(5):1881-1894.
                      doi: 10.1007/s10653-018-0237-2pubmed: 30671690google scholar: lookup
                    9. Cerling TE, Andanje SA, Gakuya F, Kariuki JM, Kariuki L, Kingoo JW, Khayale C, Lekolool I, Macharia AN, Anderson CR, Fernandez DP, Hu L, Thomas SJ. Stable isotope ecology of black rhinos (Diceros bicornis) in Kenya. Oecologia 2018 Aug;187(4):1095-1105.
                      doi: 10.1007/s00442-018-4185-4pubmed: 29955983google scholar: lookup
                    10. Hammes V, Nüsse O, Isselstein J, Kayser M. Using 13C in cattle hair to trace back the maize level in the feeding regime-A field test. PLoS One 2017;12(11):e0188926.
                      doi: 10.1371/journal.pone.0188926pubmed: 29182681google scholar: lookup
                    11. Kaczensky P, Burnik Šturm M, Sablin MV, Voigt CC, Smith S, Ganbaatar O, Balint B, Walzer C, Spasskaya NN. Stable isotopes reveal diet shift from pre-extinction to reintroduced Przewalski's horses. Sci Rep 2017 Jul 20;7(1):5950.
                      doi: 10.1038/s41598-017-05329-6pubmed: 28729625google scholar: lookup
                    12. Burnik Šturm M, Ganbaatar O, Voigt CC, Kaczensky P. Sequential stable isotope analysis reveals differences in multi-year dietary history of three sympatric equid species in SW Mongolia. J Appl Ecol 2017 Aug;54(4):1110-1119.
                      doi: 10.1111/1365-2664.12825pubmed: 28717255google scholar: lookup
                    13. Eisenmann P, Fry B, Mazumder D, Jacobsen G, Holyoake CS, Coughran D, Bengtson Nash S. Radiocarbon as a Novel Tracer of Extra-Antarctic Feeding in Southern Hemisphere Humpback Whales. Sci Rep 2017 Jun 29;7(1):4366.
                      doi: 10.1038/s41598-017-04698-2pubmed: 28663586google scholar: lookup
                    14. Cerling TE, Barnette JE, Chesson LA, Douglas-Hamilton I, Gobush KS, Uno KT, Wasser SK, Xu X. Radiocarbon dating of seized ivory confirms rapid decline in African elephant populations and provides insight into illegal trade. Proc Natl Acad Sci U S A 2016 Nov 22;113(47):13330-13335.
                      doi: 10.1073/pnas.1614938113pubmed: 27821744google scholar: lookup
                    15. Chritz KL, Blumenthal SA, Cerling TE, Klingel H. Hippopotamus (H. amphibius) diet change indicates herbaceous plant encroachment following megaherbivore population collapse. Sci Rep 2016 Sep 12;6:32807.
                      doi: 10.1038/srep32807pubmed: 27616433google scholar: lookup
                    16. Rysava K, McGill RA, Matthiopoulos J, Hopcraft JG. Re-constructing nutritional history of Serengeti wildebeest from stable isotopes in tail hair: seasonal starvation patterns in an obligate grazer. Rapid Commun Mass Spectrom 2016 Jul 15;30(13):1461-8.
                      doi: 10.1002/rcm.7572pubmed: 27321833google scholar: lookup
                    17. Eisenmann P, Fry B, Holyoake C, Coughran D, Nicol S, Bengtson Nash S. Isotopic Evidence of a Wide Spectrum of Feeding Strategies in Southern Hemisphere Humpback Whale Baleen Records. PLoS One 2016;11(5):e0156698.
                      doi: 10.1371/journal.pone.0156698pubmed: 27244081google scholar: lookup
                    18. Cotton JM, Cerling TE, Hoppe KA, Mosier TM, Still CJ. Climate, CO2, and the history of North American grasses since the Last Glacial Maximum. Sci Adv 2016 Mar;2(3):e1501346.
                      doi: 10.1126/sciadv.1501346pubmed: 27051865google scholar: lookup
                    19. McCue MD, Welch KC Jr. (13)C-Breath testing in animals: theory, applications, and future directions. J Comp Physiol B 2016 Apr;186(3):265-85.
                      doi: 10.1007/s00360-015-0950-4pubmed: 26660654google scholar: lookup
                    20. O'Brien DM. Stable Isotope Ratios as Biomarkers of Diet for Health Research. Annu Rev Nutr 2015;35:565-94.
                      doi: 10.1146/annurev-nutr-071714-034511pubmed: 26048703google scholar: lookup
                    21. Lehmann D, Mfune JK, Gewers E, Brain C, Voigt CC. Individual variation of isotopic niches in grazing and browsing desert ungulates. Oecologia 2015 Sep;179(1):75-88.
                      doi: 10.1007/s00442-015-3335-1pubmed: 25953117google scholar: lookup
                    22. Loudon JE, Grobler JP, Sponheimer M, Moyer K, Lorenz JG, Turner TR. Using the stable carbon and nitrogen isotope compositions of vervet monkeys (Chlorocebus pygerythrus) to examine questions in ethnoprimatology. PLoS One 2014;9(7):e100758.
                      doi: 10.1371/journal.pone.0100758pubmed: 25010211google scholar: lookup
                    23. Remien CH, Adler FR, Chesson LA, Valenzuela LO, Ehleringer JR, Cerling TE. Deconvolution of isotope signals from bundles of multiple hairs. Oecologia 2014 Jul;175(3):781-9.
                      doi: 10.1007/s00442-014-2945-3pubmed: 24793936google scholar: lookup
                    24. Dejmal M, Lisá L, Fišáková Nývltová M, Bajer A, Petr L, Kočár P, Kočárová R, Nejman L, Rybníček M, Sůvová Z, Culp R, Vavrčík H. Medieval horse stable; the results of multi proxy interdisciplinary research. PLoS One 2014;9(3):e89273.
                      doi: 10.1371/journal.pone.0089273pubmed: 24670874google scholar: lookup
                    25. Braun A, Schneider S, Auerswald K, Bellof G, Schnyder H. Forward modeling of fluctuating dietary 13C signals to validate 13C turnover models of milk and milk components from a diet-switch experiment. PLoS One 2013;8(12):e85235.
                      doi: 10.1371/journal.pone.0085235pubmed: 24392000google scholar: lookup
                    26. Blumenthal SA, Chritz KL, Rothman JM, Cerling TE. Detecting intraannual dietary variability in wild mountain gorillas by stable isotope analysis of feces. Proc Natl Acad Sci U S A 2012 Dec 26;109(52):21277-82.
                      doi: 10.1073/pnas.1215782109pubmed: 23236160google scholar: lookup
                    27. O'Connell TC, Kneale CJ, Tasevska N, Kuhnle GG. The diet-body offset in human nitrogen isotopic values: a controlled dietary study. Am J Phys Anthropol 2012 Nov;149(3):426-34.
                      doi: 10.1002/ajpa.22140pubmed: 23042579google scholar: lookup
                    28. Wurster CM, Robertson J, Westcott DA, Dryden B, Zazzo A, Bird MI. Utilization of sugarcane habitat by feral pig (Sus scrofa) in northern tropical Queensland: evidence from the stable isotope composition of hair. PLoS One 2012;7(9):e43538.
                      doi: 10.1371/journal.pone.0043538pubmed: 22957029google scholar: lookup
                    29. Codron D, Sponheimer M, Codron J, Newton I, Lanham JL, Clauss M. The confounding effects of source isotopic heterogeneity on consumer-diet and tissue-tissue stable isotope relationships. Oecologia 2012 Aug;169(4):939-53.
                      doi: 10.1007/s00442-012-2274-3pubmed: 22349754google scholar: lookup
                    30. Lecomte N, Ahlstrøm O, Ehrich D, Fuglei E, Ims RA, Yoccoz NG. Intrapopulation variability shaping isotope discrimination and turnover: experimental evidence in arctic foxes. PLoS One 2011;6(6):e21357.
                      doi: 10.1371/journal.pone.0021357pubmed: 21731715google scholar: lookup
                    31. Yeung EH, Saudek CD, Jahren AH, Kao WH, Islas M, Kraft R, Coresh J, Anderson CA. Evaluation of a novel isotope biomarker for dietary consumption of sweets. Am J Epidemiol 2010 Nov 1;172(9):1045-52.
                      doi: 10.1093/aje/kwq247pubmed: 20817784google scholar: lookup
                    32. Downey P. Profile of Thure E. Cerling. Proc Natl Acad Sci U S A 2010 Aug 31;107(35):15326-8.
                      doi: 10.1073/pnas.1010204107pubmed: 20696920google scholar: lookup
                    33. Butz DE, Cook ME, Eghbalnia HR, Assadi-Porter F, Porter WP. Changes in the natural abundance of 13CO2/12CO2 in breath due to lipopolysacchride-induced acute phase response. Rapid Commun Mass Spectrom 2009 Dec;23(23):3729-35.
                      doi: 10.1002/rcm.4310pubmed: 19902411google scholar: lookup
                    34. Cerling TE, Wittemyer G, Ehleringer JR, Remien CH, Douglas-Hamilton I. History of Animals using Isotope Records (HAIR): a 6-year dietary history of one family of African elephants. Proc Natl Acad Sci U S A 2009 May 19;106(20):8093-100.
                      doi: 10.1073/pnas.0902192106pubmed: 19365077google scholar: lookup
                    35. Voigt CC, Rex K, Michener RH, Speakman JR. Nutrient routing in omnivorous animals tracked by stable carbon isotopes in tissue and exhaled breath. Oecologia 2008 Aug;157(1):31-40.
                      doi: 10.1007/s00442-008-1057-3pubmed: 18496717google scholar: lookup
                    36. Guelinckx J, Maes J, Geysen B, Ollevier F. Estuarine recruitment of a marine goby reconstructed with an isotopic clock. Oecologia 2008 Aug;157(1):41-52.
                      doi: 10.1007/s00442-008-1045-7pubmed: 18481098google scholar: lookup
                    37. Martínez Del Rio C, Anderson-Sprecher R. Beyond the reaction progress variable: the meaning and significance of isotopic incorporation data. Oecologia 2008 Jul;156(4):765-72.
                      doi: 10.1007/s00442-008-1040-zpubmed: 18446374google scholar: lookup
                    38. Ehleringer JR, Bowen GJ, Chesson LA, West AG, Podlesak DW, Cerling TE. Hydrogen and oxygen isotope ratios in human hair are related to geography. Proc Natl Acad Sci U S A 2008 Feb 26;105(8):2788-93.
                      doi: 10.1073/pnas.0712228105pubmed: 18299562google scholar: lookup
                    39. Voigt CC, Grasse P, Rex K, Hetz SK, Speakman JR. Bat breath reveals metabolic substrate use in free-ranging vampires. J Comp Physiol B 2008 Jan;178(1):9-16.
                      doi: 10.1007/s00360-007-0194-zpubmed: 17701185google scholar: lookup
                    40. Popa-Lisseanu AG, Delgado-Huertas A, Forero MG, Rodríguez A, Arlettaz R, Ibáñez C. Bats' conquest of a formidable foraging niche: the myriads of nocturnally migrating songbirds. PLoS One 2007 Feb 14;2(2):e205.
                      doi: 10.1371/journal.pone.0000205pubmed: 17299585google scholar: lookup
                    41. Cerling TE, Ayliffe LK, Dearing MD, Ehleringer JR, Passey BH, Podlesak DW, Torregrossa AM, West AG. Determining biological tissue turnover using stable isotopes: the reaction progress variable. Oecologia 2007 Mar;151(2):175-89.
                      doi: 10.1007/s00442-006-0571-4pubmed: 17186277google scholar: lookup
                    42. MacAvoy SE, Arneson LS, Bassett E. Correlation of metabolism with tissue carbon and nitrogen turnover rate in small mammals. Oecologia 2006 Nov;150(2):190-201.
                      doi: 10.1007/s00442-006-0522-0pubmed: 16967272google scholar: lookup
                    43. McIntyre PB, Flecker AS. Rapid turnover of tissue nitrogen of primary consumers in tropical freshwaters. Oecologia 2006 May;148(1):12-21.
                      doi: 10.1007/s00442-005-0354-3pubmed: 16456686google scholar: lookup
                    44. Cerling TE, Wittemyer G, Rasmussen HB, Vollrath F, Cerling CE, Robinson TJ, Douglas-Hamilton I. Stable isotopes in elephant hair document migration patterns and diet changes. Proc Natl Acad Sci U S A 2006 Jan 10;103(2):371-3.
                      doi: 10.1073/pnas.0509606102pubmed: 16407164google scholar: lookup
                    45. Gratton C, Forbes AE. Changes in delta 13C stable isotopes in multiple tissues of insect predators fed isotopically distinct prey. Oecologia 2006 Apr;147(4):615-24.
                      doi: 10.1007/s00442-005-0322-ypubmed: 16341886google scholar: lookup
                    46. Phillips DL, Eldridge PM. Estimating the timing of diet shifts using stable isotopes. Oecologia 2006 Mar;147(2):195-203.
                      doi: 10.1007/s00442-005-0292-0pubmed: 16341714google scholar: lookup
                    47. Dalerum F, Angerbjörn A. Resolving temporal variation in vertebrate diets using naturally occurring stable isotopes. Oecologia 2005 Aug;144(4):647-58.
                      doi: 10.1007/s00442-005-0118-0pubmed: 16041545google scholar: lookup
                    48. Bowen GJ, Wassenaar LI, Hobson KA. Global application of stable hydrogen and oxygen isotopes to wildlife forensics. Oecologia 2005 Apr;143(3):337-48.
                      doi: 10.1007/s00442-004-1813-ypubmed: 15726429google scholar: lookup
                    49. Podlesak DW, McWilliams SR, Hatch KA. Stable isotopes in breath, blood, feces and feathers can indicate intra-individual changes in the diet of migratory songbirds. Oecologia 2005 Feb;142(4):501-10.
                      doi: 10.1007/s00442-004-1737-6pubmed: 15586297google scholar: lookup
                    50. Shipley ON, Cerling TE, Wittemyer G, Lübcker N, Newsome SD. Repeated migration informs amino acid nitrogen isotope incorporation in the African elephant Loxodonta africana. Oecologia 2026 Feb 3;208(2):27.
                      doi: 10.1007/s00442-026-05862-0pubmed: 41634248google scholar: lookup
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                      doi: 10.1098/rsos.250283pubmed: 40535935google scholar: lookup
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                      doi: 10.1038/s42003-025-07686-9pubmed: 39984748google scholar: lookup
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                      doi: 10.1093/cz/zoae007pubmed: 39678826google scholar: lookup
                    54. Kabalika Z, Haydon DT, McGill RAR, Morales JM, Morrison TA, Newton J, Hopcraft JGC. Using sulfur stable isotope ratios (δ(34) S) for animal geolocation: Estimating the delay mechanisms between diet ingestion and isotope incorporation in tail hair. Rapid Commun Mass Spectrom 2024 Jan 30;38(2):e9674.
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