FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Infection and immunity1976; 13(1); 273-280; doi: 10.1128/iai.13.1.273-280.1976

Ultrastructural observations on Ehrlichia equi organisms in equine granulocytes.

Abstract: The ultrastructure of the etiological agent of equine ehrlichiosis, Ehrlichia equi, was studied in equine peripheral leukocytes. The organisms occurred within membrane-lined cytoplasmic vacuoles of neutrophils and eosinophils. Ovoid, round, and rod-shaped profiles were observed. From 1 to 33 organisms were present in a thin-section profile of a cytoplasmic vacuole. Many cells contained multiple organism-containing vacuoles. The organisms had a cell wall and plasma membrane, and internally they consisted of electron-dense and lucid areas. A great variation in size was observed. The morphological features were most consistent with agents of the genus Ehrlichia.
Get Full Text
Publication Date: 1976-01-11 PubMed ID: 1248873PubMed Central: PMC420604DOI: 10.1128/iai.13.1.273-280.1976Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper focuses on studying the ultrastructure of Ehrlichia equi, the causative agent of equine ehrlichiosis, in equine leukocytes. It details how the organisms appear in the cytoplasmic vacuoles of certain white blood cells and describes their physical characteristics, variability in size, and correlation to other species in the Ehrlichia genus.

Study on Ehrlichia Equi

  • This research paper investigates the etiological agent Ehrlichia equi of equine ehrlichiosis. Ehrlichiosis is a tick-borne disease of horses that can lead to severe clinical signs, including high fever, depression, anorexia, and thrombocytopenia.
  • The study focuses on conducting a detailed observation of the ultrastructure of Ehrlichia equi in equine peripheral leukocytes, a type of white blood cell.

Organisms in Leukocytes

  • The organisms were found within membrane-lined vacuoles present in the cytoplasm of neutrophils and eosinophils – types of granulocytes, which are a type of white blood cell integral to the immune system.
  • Ovoid, round, and rod-shaped profiles of the organisms were observed within these vacuoles. In a thin-section profile of a cytoplasmic vacuole, there were found to be from 1 to 33 organisms present.
  • A significant finding was that many of the studied cells contained multiple vacuoles, each housing the Ehrlichia equi organisms.

Morphology of the Organisms

  • The Ehrlichia equi organisms have a distinct cell wall and plasma membrane. Within this formation, electron-dense and lucid areas were observed, showcasing the internal composition of these organisms.
  • There was a substantial variation in the size of these organisms observed. Despite this variation, the overall morphology was found to be most consistent with other agents of the genus Ehrlichia.

Cite This Article

APA
Sells DM, Hildebrandt PK, Lewis GE, Nyindo MB, Ristic M. (1976). Ultrastructural observations on Ehrlichia equi organisms in equine granulocytes. Infect Immun, 13(1), 273-280. https://doi.org/10.1128/iai.13.1.273-280.1976

Publication

Infection and immunity
ISSN: 0019-9567
NlmUniqueID: 0246127
Country: United States
Language: English
Volume: 13
Issue: 1
Pages: 273-280

Researcher Affiliations

Sells, D M
    Hildebrandt, P K
      Lewis, G E
        Nyindo, M B
          Ristic, M

            MeSH Terms

            • Animals
            • Ehrlichia / ultrastructure
            • Eosinophils / ultrastructure
            • Granulocytes / ultrastructure
            • Horses
            • Leukocytes / ultrastructure
            • Neutrophils / ultrastructure
            • Rickettsiaceae / ultrastructure

            References

            This article includes 14 references
            1. Krauss H, Davies FG, Odegaard OA, Cooper JE. The morphology of the causal agent of bovine petechial fever (Ondiri disease).. J Comp Pathol 1972 Jul;82(3):241-6.
              pubmed: 4342350doi: 10.1016/0021-9975(72)90002-3google scholar: lookup
            2. Simpson CF. Relationship of Ehrlichia canis-infected mononuclear cells to blood vessels of lungs.. Infect Immun 1974 Sep;10(3):590-6.
              pubmed: 4372174doi: 10.1128/iai.10.3.590-596.1974google scholar: lookup
            3. Armstrong JA. Relation of PLT organisms to the rickettsiae, with special reference to structure and multiplication.. Acta Virol 1968 Jan;12(1):15-7.
              pubmed: 4384103
            4. Hildebrandt PK, Conroy JD, McKee AE, Nyindo MB, Huxsoll DL. Ultrastructure of Ehrlichia canis.. Infect Immun 1973 Feb;7(2):265-71.
              pubmed: 4735376doi: 10.1128/iai.7.2.265-271.1973google scholar: lookup
            5. Stannard AA, Gribble DH, Smith RS. Equine ehrlichiosis: a disease with similarities to tick-borne fever and bovine petechial fever.. Vet Rec 1969 Feb 8;84(6):149-50.
              pubmed: 5818868doi: 10.1136/vr.84.6.149google scholar: lookup
            6. Gribble DH. Equine ehrlichiosis.. J Am Vet Med Assoc 1969 Jul 15;155(2):462-9.
              pubmed: 5819585
            7. Cutlip RC. Electron microscopy of cell cultures infected with a chlamydial agent causing polyarthritis of lambs.. Infect Immun 1970 May;1(5):499-502.
              pubmed: 16557765doi: 10.1128/iai.1.5.499-502.1970google scholar: lookup
            8. HIGASHI N. ELECTRON MICROSCOPIC STUDIES ON THE MODE OF REPRODUCTION OF TRACHOMA VIRUS AND PSITTACOSIS VIRUS IN CELL CULTURES.. Exp Mol Pathol 1965 Feb;4:24-39.
              pubmed: 14297551doi: 10.1016/0014-4800(65)90021-3google scholar: lookup
            9. Lewis GE Jr, Huxsoll DL, Ristic M, Johnson AJ. Experimentally induced infection of dogs, cats, and nonhuman primates with Ehrlichia equi, etiologic agent of equine ehrlichiosis.. Am J Vet Res 1975 Jan;36(1):85-8.
              pubmed: 1115421
            10. Simpson CF. Structure of Ehrlichia canis in blood monocytes of a dog.. Am J Vet Res 1972 Dec;33(12):2451-4.
              pubmed: 4641202
            11. Anderson DR, Hopps HE, Barile MF, Bernheim BC. Comparison of the ultrastructure of several rickettsiae, ornithosis virus, and Mycoplasma in tissue culture.. J Bacteriol 1965 Nov;90(5):1387-404.
              pubmed: 4954556doi: 10.1128/jb.90.5.1387-1404.1965google scholar: lookup
            12. Tuomi J, von Bonsdorff CH. Electron microscopy of tick-borne fever agent in bovine and ovine phagocytizing leukocytes.. J Bacteriol 1966 Nov;92(5):1478-92.
              pubmed: 5924275doi: 10.1128/jb.92.5.1478-1492.1966google scholar: lookup
            13. LOCKWOOD WR, ALLISON F Jr. ELECTRON MICROGRAPHIC STUDIES OF PHAGOCYTIC CELLS II. OBSERVATIONS ON THE CHANGES INDUCED IN THE CYTOPLASMIC CONTENTS OF HUMAN GRANULOCYTES BY THE INGESTION OF ROUGH PNEUMOCOCUS.. Br J Exp Pathol 1964 Jun;45(3):294-9.
              pubmed: 14173171
            14. MITSUI Y, FUJIMOTO M, KAJIMA M. DEVELOPMENT AND MORPHOLOGY OF TRACHOMA AGENT IN THE YOLK SAC CELL AS REVEALED BY ELECTRON MICROSCOPY.. Virology 1964 May;23:30-45.
              pubmed: 14157503doi: 10.1016/s0042-6822(64)80005-2google scholar: lookup

            Citations

            This article has been cited 5 times.
            1. Teodorowski O, Kalinowski M, Winiarczyk D, Janecki R, Winiarczyk S, Adaszek Ł. Molecular surveillance of tick-borne diseases affecting horses in Poland-Own observations. Vet Med Sci 2021 Jul;7(4):1159-1165.
              doi: 10.1002/vms3.451pubmed: 33620135google scholar: lookup
            2. Tate CM, Howerth EW, Mead DG, Dugan VG, Luttrell MP, Sahora AI, Munderloh UG, Davidson WR, Yabsley MJ. Anaplasma odocoilei sp. nov. (family Anaplasmataceae) from white-tailed deer (Odocoileus virginianus). Ticks Tick Borne Dis 2013 Feb;4(1-2):110-9.
              doi: 10.1016/j.ttbdis.2012.09.005pubmed: 23276749google scholar: lookup
            3. Dedonder SE, Cheng C, Willard LH, Boyle DL, Ganta RR. Transmission electron microscopy reveals distinct macrophage- and tick cell-specific morphological stages of Ehrlichia chaffeensis. PLoS One 2012;7(5):e36749.
              doi: 10.1371/journal.pone.0036749pubmed: 22615806google scholar: lookup
            4. Holland CJ, Ristic M, Huxsoll DL, Cole AI, Rapmund G. Adaptation of Ehrlichia sennetsu to canine blood monocytes: preliminary structural and serological studies with cell culture-derived Ehrlichia sennetsu. Infect Immun 1985 May;48(2):366-71.
              doi: 10.1128/iai.48.2.366-371.1985pubmed: 2985504google scholar: lookup
            5. Gehlen H, Inerle K, Bartel A, Stöckle SD, Ulrich S, Briese B, Straubinger RK. Seroprevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum Infections in German Horses. Animals (Basel) 2023 Jun 14;13(12).
              doi: 10.3390/ani13121984pubmed: 37370494google scholar: lookup
            Subscribe to our newsletter
            Join 99,780 horse owners like you who receive our email updates on horse health and nutrition.