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Home / Equine Research Bank / J Equine Sci / Undetectable vitamin D3 in equine skin irradiated with ultra...
Journal of equine science2022; 33(3); 45-49; doi: 10.1294/jes.33.45

Undetectable vitamin D3 in equine skin irradiated with ultraviolet light.

Abstract: Vitamin D requirements for most animals are expected to be fulfilled through daily exposure of the skin to solar ultraviolet B radiation. The synthesis of vitamin D in skin depends on different factors including melanin pigmentation, the amount of UVB radiation reaching the skin, type of clothing/hair coat, latitude and altitude, season, and time of day. Alternatively vitamin D may be obtained from UVB irradiated pasture species. Recent studies have shown that in unsupplemented grazing horses 25-hydroxyvitamin D is the predominant form of vitamin D in plasma, and that 25OHD is undetectable suggesting horses may rely on diet to obtain vitamin D. In order to mimic the natural environment of skin to sunlight exposure, five equine and two ovine devitalized skin samples were irradiated with 5 J/cm of UVB light followed by measurement of 7-dehydrocholesterol (7-DHC) and vitamin D concentrations using reverse-phase high pressure liquid chromatography (HPLC). HPLC revealed the presence of 7-DHC in the skin of both horses and sheep. Vitamin D was undetectable in both ovine and equine skin prior to irradiation, but after irradiation with UVB light, ovine skin showed an increase in vitamin D concentration (mean 0.16 ± 0.07 µg/g), whereas vitamin D was undetectable in equine skin. These results provide additional evidence that horses make negligible quantities of vitamin D in their skin after exposure to UVB light and may therefore rely on their diet as a primary source of vitamin D.
©2022 The Japanese Society of Equine Science.
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Publication Date: 2022-09-21 PubMed ID: 36196139PubMed Central: PMC9522623DOI: 10.1294/jes.33.45Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the relationship between skin exposure to ultraviolet radiation and vitamin D synthesis in the skin of horses. The study proposes that horses depend on their diet for vitamin D, as their skin makes negligible quantities of the vitamin after exposure to UVB light.

Research Purpose

  • The study aimed at understanding how equine skin reacts to exposure to ultraviolet B radiation (UVB) light and its subsequent role in the creation of vitamin D.
  • Additionally, it aimed to explore the hypothesis that horses predominantly rely on their diet, rather than sunlight exposure, to meet their vitamin D needs.

Methods and Materials

  • Five equine (horse) and two ovine (sheep) devitalized skin samples were used for this study.
  • The skin samples were exposed to 5 J/cm of UVB light to simulate the natural environment of skin to sunlight exposure.
  • Following the exposure, the concentration of 7-dehydrocholesterol (7-DHC) and vitamin D was measured using reverse-phase high-pressure liquid chromatography (HPLC).

Results

  • The presence of 7-DHC, which is an essential substance for the production of vitamin D, was revealed in the skin of both horses and sheep through HPLC.
  • Vitamin D was undetectable in both ovine and equine skin before irradiation.
  • However, post-irradiation, the ovine skin showed an increase in Vitamin D concentration (mean 0.16 ± 0.07 µg/g), while Vitamin D remained undetectable in the equine skin.

Conclusion

  • The study provides evidence supporting the theory that horses produce negligible quantities of Vitamin D in their skin after UVB light exposure.
  • Hence, it suggests that horses may rely on their diet as a primary source of Vitamin D.

Cite This Article

APA
Azarpeykan S, Gee EK, Thompson KG, Dittmer KE. (2022). Undetectable vitamin D3 in equine skin irradiated with ultraviolet light. J Equine Sci, 33(3), 45-49. https://doi.org/10.1294/jes.33.45

Publication

Journal of equine science
ISSN: 1340-3516
NlmUniqueID: 9503751
Country: Japan
Language: English
Volume: 33
Issue: 3
Pages: 45-49

Researcher Affiliations

Azarpeykan, Sara
  • School of Veterinary Science, Tennent Drive, Massey University, Palmerston North 4442, New Zealand.
Gee, Erica K
  • School of Veterinary Science, Tennent Drive, Massey University, Palmerston North 4442, New Zealand.
Thompson, Keith G
  • School of Veterinary Science, Tennent Drive, Massey University, Palmerston North 4442, New Zealand.
Dittmer, Keren E
  • School of Veterinary Science, Tennent Drive, Massey University, Palmerston North 4442, New Zealand.

References

This article includes 24 references
  1. Azarpeykan S, Dittmer KE, Gee EK, Marshall JC, Elder P, Acke E, Thompson KG. Circadian rhythm of calciotropic hormones, serum calcium, phosphorus and magnesium during the shortest and longest days of the year in horses in New Zealand.. J Anim Physiol Anim Nutr (Berl) 2016 Dec;100(6):1058-1066.
    pubmed: 26841283doi: 10.1111/jpn.12477google scholar: lookup
  2. Azarpeykan S, Dittmer KE, Gee EK, Marshall JC, Wallace J, Elder P, Acke E, Thompson KG. Influence of blanketing and season on vitamin D and parathyroid hormone, calcium, phosphorus, and magnesium concentrations in horses in New Zealand.. Domest Anim Endocrinol 2016 Jul;56:75-84.
    pubmed: 27131337doi: 10.1016/j.domaniend.2016.03.003google scholar: lookup
  3. Chaudhary MS, Care AD. Production of vitamin D3 in sheep in response to artificial ultraviolet light exposure. Proceedings of the 6th workshop on Vitamin D, Merano, Italy: Vitamin D: A Chemical, Biochemical and Clinical Update 6th Workshop: 711–2.
  4. Chen TC, Chimeh F, Lu Z, Mathieu J, Person KS, Zhang A, Kohn N, Martinello S, Berkowitz R, Holick MF. Factors that influence the cutaneous synthesis and dietary sources of vitamin D.. Arch Biochem Biophys 2007 Apr 15;460(2):213-7.
    pmc: PMC2698590pubmed: 17254541doi: 10.1016/j.abb.2006.12.017google scholar: lookup
  5. DeLuca HF. Overview of general physiologic features and functions of vitamin D.. Am J Clin Nutr 2004 Dec;80(6 Suppl):1689S-96S.
    pubmed: 15585789doi: 10.1093/ajcn/80.6.1689sgoogle scholar: lookup
  6. Dittmer KE, Thompson KG. Vitamin D metabolism and rickets in domestic animals: a review.. Vet Pathol 2011 Mar;48(2):389-407.
    pubmed: 20634407doi: 10.1177/0300985810375240google scholar: lookup
  7. Ganmaa D, Holick MF, Rich-Edwards JW, Frazier LA, Davaalkham D, Ninjin B, Janes C, Hoover RN, Troisi R. Vitamin D deficiency in reproductive age Mongolian women: a cross sectional study.. J Steroid Biochem Mol Biol 2014 Jan;139:1-6.
    pmc: PMC3909713pubmed: 24076033doi: 10.1016/j.jsbmb.2013.09.011google scholar: lookup
  8. Holick MF. The cutaneous photosynthesis of previtamin D3: a unique photoendocrine system.. J Invest Dermatol 1981 Jul;77(1):51-8.
    pubmed: 6265564doi: 10.1111/1523-1747.ep12479237google scholar: lookup
  9. Holick MF. Sunlight and vitamin D for bone health and prevention of autoimmune diseases, cancers, and cardiovascular disease.. Am J Clin Nutr 2004 Dec;80(6 Suppl):1678S-88S.
    pubmed: 15585788doi: 10.1093/ajcn/80.6.1678sgoogle scholar: lookup
  10. Holick MF, Chen TC, Lu Z, Sauter E. Vitamin D and skin physiology: a D-lightful story.. J Bone Miner Res 2007 Dec;22 Suppl 2:V28-33.
    pubmed: 18290718doi: 10.1359/jbmr.07s211google scholar: lookup
  11. How KL, Hazewinkel HA, Mol JA. Dietary vitamin D dependence of cat and dog due to inadequate cutaneous synthesis of vitamin D.. Gen Comp Endocrinol 1994 Oct;96(1):12-8.
    pubmed: 7843559doi: 10.1006/gcen.1994.1154google scholar: lookup
  12. Hymøller L, Jensen SK. Vitamin D(3) synthesis in the entire skin surface of dairy cows despite hair coverage.. J Dairy Sci 2010 May;93(5):2025-9.
    pubmed: 20412916doi: 10.3168/jds.2009-2991google scholar: lookup
  13. Hymøller L, Jensen SK. Vitamin D analysis in plasma by high performance liquid chromatography (HPLC) with C(30) reversed phase column and UV detection--easy and acetonitrile-free.. J Chromatogr A 2011 Apr 8;1218(14):1835-41.
    pubmed: 21342692doi: 10.1016/j.chroma.2011.02.004google scholar: lookup
  14. Judson GJ, McGregor BA, Howse AM. Blood mineral, trace-element and vitamin concentrations in Huacaya alpacas and Merino sheep grazing the same pasture. Anim. Prod. Sci. 51: 873.
  15. Kale MS, Dittmer KE, Roe WD, Gartrell BD. Interspecies differences in plasma concentrations of 25-hydroxyvitamin D3 and dermal Vitamin D synthesis of kiwi (Apteryx mantelli), tuatara (Sphenodon punctatus), and New Zealand sea lions (Phocarctos hookeri).. J Comp Physiol B 2018 Mar;188(2):325-331.
    pubmed: 28755031doi: 10.1007/s00360-017-1117-2google scholar: lookup
  16. Kenny DE, Irlbeck NA, Chen TC, Lu Z, Holick MF. Determination of vitamins D, A, and E in sera and vitamin D in milk from captive and free-ranging Polar Bears (Ursus maritimus), and 7-dehydrocholesterol levels in skin from captive Polar Bears. Zoo Biol. 17: 285–293.
  17. Kohler M, Leiber F, Willems H, Merbold L, Liesegang A. Influence of altitude on vitamin D and bone metabolism of lactating sheep and goats.. J Anim Sci 2013 Nov;91(11):5259-68.
    pubmed: 24045489doi: 10.2527/jas.2013-6702google scholar: lookup
  18. Kovács S, Wilkens MR, Liesegang A. Influence of UVB exposure on the vitamin D status and calcium homoeostasis of growing sheep and goats.. J Anim Physiol Anim Nutr (Berl) 2015 Apr;99 Suppl S1:1-12.
    pubmed: 25865417doi: 10.1111/jpn.12311google scholar: lookup
  19. Lips P. Vitamin D physiology.. Prog Biophys Mol Biol 2006 Sep;92(1):4-8.
    pubmed: 16563471doi: 10.1016/j.pbiomolbio.2006.02.016google scholar: lookup
  20. Morris JG. Ineffective vitamin D synthesis in cats is reversed by an inhibitor of 7-dehydrocholestrol-delta7-reductase.. J Nutr 1999 Apr;129(4):903-8.
    pubmed: 10203568doi: 10.1093/jn/129.4.903google scholar: lookup
  21. Otani S, Majbauddin A, Qing Q, Intekhab A, Susa R, Fujitani Y, Masumoto T, Amano H, Kurozawa Y, Davaalkham D. Spatial epidemiology of vitamin d status in Mongolia. Environ. Epidemiol. 3: 298.
  22. Solounias N, Danowitz M, Stachtiaris E, Khurana A, Araim M, Sayegh M, Natale J. The evolution and anatomy of the horse manus with an emphasis on digit reduction.. R Soc Open Sci 2018 Jan;5(1):171782.
    pmc: PMC5792948pubmed: 29410871doi: 10.1098/rsos.171782google scholar: lookup
  23. Tian XQ, Chen TC, Matsuoka LY, Wortsman J, Holick MF. Kinetic and thermodynamic studies of the conversion of previtamin D3 to vitamin D3 in human skin.. J Biol Chem 1993 Jul 15;268(20):14888-92.
    pubmed: 8392061
  24. Weinstock J, Willerslev E, Sher A, Tong W, Ho SY, Rubenstein D, Storer J, Burns J, Martin L, Bravi C, Prieto A, Froese D, Scott E, Xulong L, Cooper A. Evolution, systematics, and phylogeography of pleistocene horses in the new world: a molecular perspective.. PLoS Biol 2005 Aug;3(8):e241.
    pmc: PMC1159165pubmed: 15974804doi: 10.1371/journal.pbio.0030241google scholar: lookup

Citations

This article has been cited 6 times.
  1. Dosi MCM, Riggs CM, May J, Lee A, Cillan-Garcia E, Pagan J, McGorum BC. Thoroughbred Racehorses in Hong Kong Require Vitamin D Supplementation to Mitigate the Risk of Low Vitamin D Status. Animals (Basel) 2023 Jun 29;13(13).
    doi: 10.3390/ani13132145pubmed: 37443942google scholar: lookup
  2. Bjørklund G, Butnariu M, Gurgas L, Hangan T. Vitamin D and Diabetes: Exploring the Link, Prevention, and Management. Curr Med Chem 2026;33(4):689-707.
    doi: 10.2174/0109298673360987250621042131pubmed: 40641014google scholar: lookup
  3. Bruins-van Sonsbeek LGR, Verschuren MCM, Kaal S, Lindenburg PW, Rodenburg KCW, Clauss M, Speksnijder AGCL, Rutten VPMG, Bonnet BFJ, Wittink F. Rhinoceromics: a multi-amplicon study with clinical markers to transferrin saturation levels in ex-situ black rhinoceros (Diceros bicornis michaeli). Front Microbiol 2025;16:1515939.
    doi: 10.3389/fmicb.2025.1515939pubmed: 40510676google scholar: lookup
  4. Alemi M, Ahmadi Sheikhsarmast S, Mohri M. Serum 25(OH) Vitamin D Concentrations in Horses: Effects of Age, Gender, Breed, Skin Colour and Season. Vet Med Sci 2025 Jan;11(1):e70092.
    doi: 10.1002/vms3.70092pubmed: 39778002google scholar: lookup
  5. Bruins-van Sonsbeek LGR, Corbee RJ. Serum 25(OH)D Analysis in Captive Pachyderms (Loxodonta africana, Elephas maximus, Diceros bicornis, Rhinoceros unicornis, Tapirus indicus) in Europe. Animals (Basel) 2024 Oct 2;14(19).
    doi: 10.3390/ani14192843pubmed: 39409792google scholar: lookup
  6. Orzołek A, Rafalska KT, Domosławska-Wyderska A, Rafalska AM, Dziekońska A, Jastrzębska E, Dobbek D. The effect of solarium light therapy on selected biological and biochemical parameters of peripheral blood in young and old horses. PLoS One 2024;19(5):e0304290.
    doi: 10.1371/journal.pone.0304290pubmed: 38787841google scholar: lookup
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