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PloS one2014; 9(3); e91684; doi: 10.1371/journal.pone.0091684

Unraveling the equine lymphocyte proteome: differential septin 7 expression associates with immune cells in equine recurrent uveitis.

Abstract: Equine recurrent uveitis is a spontaneous, lymphocyte-driven autoimmune disease. It affects horses worldwide and presents with painful remitting-relapsing inflammatory attacks of inner eye structures eventually leading to blindness. Since lymphocytes are the key players in equine recurrent uveitis, we were interested in potential changes of their protein repertoire which may be involved in disease pathogenesis. To create a reference for differential proteome analysis, we first unraveled the equine lymphocyte proteome by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subsequently identified 352 protein spots. Next, we compared lymphocytes from ERU cases and healthy horses with a two-dimensional fluorescence difference in gel electrophoresis approach. With this technique, we identified seven differentially expressed proteins between conditions. One of the significantly lower expressed candidates, septin 7, plays a role in regulation of cell shape, motility and migration. Further analyses revealed T cells as the main cell type with decreased septin 7 abundance in equine recurrent uveitis. These findings point to a possible pathogenetic role of septin 7 in this sight-threatening disease.
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Publication Date: 2014-03-10 PubMed ID: 24614191PubMed Central: PMC3951111DOI: 10.1371/journal.pone.0091684Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores the differences in protein expression within the immune cells of horses suffering from equine recurrent uveitis (ERU), an autoimmune disease that can lead to blindness, and healthy horses. In particular, the study found that the protein septin 7, which influences cell shape, movement and migration, was less prevalent in the immune cells of horses with ERU.

Study Purpose and Methods

  • The primary aim of this research was to ascertain the changes that occur in the protein repertoire of lymphocytes, which are crucial players in ERU. This was pursued with the hope of better understanding the pathogenesis, or the biological mechanism leading to the disease development, of ERU.
  • The researchers first established a reference for differential proteome analysis by revealing the equine lymphocyte proteome, using a technique known as two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and identifying 352 protein spots.
  • Having identified the baseline proteome, the scientists then compared the lymphocytes from ERU-afflicted horses and healthy horses using another method called two-dimensional fluorescence difference in gel electrophoresis.

Findings

  • Seven proteins were found to have different levels of expression between the ERU and healthy cases. The examination of these proteins could give insights into the alteration of the immune response in ERU.
  • Specifically, one of the proteins called septin 7, was found significantly less in ERU cases. Septin 7 is important in regulating a cell’s shape, its ability to move, and its migration. Lower levels of septin 7 were discovered mainly within the T cells of the immune system.

Implications

  • The researchers suggest that the lower abundance of septin 7 may play a key role in the development of ERU.
  • This new information could contribute significantly to the understanding of the molecular changes that occur in ERU, paving the way for the development of new treatments or preventative measures for this sight-threatening disease.

Cite This Article

APA
Degroote RL, Hauck SM, Amann B, Hirmer S, Ueffing M, Deeg CA. (2014). Unraveling the equine lymphocyte proteome: differential septin 7 expression associates with immune cells in equine recurrent uveitis. PLoS One, 9(3), e91684. https://doi.org/10.1371/journal.pone.0091684

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 9
Issue: 3
Pages: e91684

Researcher Affiliations

Degroote, Roxane L
  • Institute of Animal Physiology, Department of Veterinary Sciences, Ludwig Maximilians University Munich, Munich, Germany.
Hauck, Stefanie M
  • Research Unit Protein Sciences, Helmholtz Center Munich, German Research Center for Environmental Health, Neuherberg, Germany.
Amann, Barbara
  • Institute of Animal Physiology, Department of Veterinary Sciences, Ludwig Maximilians University Munich, Munich, Germany.
Hirmer, Sieglinde
  • Institute of Animal Physiology, Department of Veterinary Sciences, Ludwig Maximilians University Munich, Munich, Germany.
Ueffing, Marius
  • Research Unit Protein Sciences, Helmholtz Center Munich, German Research Center for Environmental Health, Neuherberg, Germany; Center for Ophthalmology, Institute for Ophthalmic Research, Eberhard Karls University of Tübingen, Tübingen, Germany.
Deeg, Cornelia A
  • Institute of Animal Physiology, Department of Veterinary Sciences, Ludwig Maximilians University Munich, Munich, Germany.

MeSH Terms

  • Animals
  • Blotting, Western
  • Electrophoresis, Gel, Two-Dimensional
  • Flow Cytometry
  • Horse Diseases / immunology
  • Horses / immunology
  • Lymphocyte Subsets / metabolism
  • Mass Spectrometry
  • Proteome / metabolism
  • Recurrence
  • Septins / metabolism
  • T-Lymphocytes / metabolism
  • Uveitis / immunology
  • Uveitis / veterinary

Conflict of Interest Statement

Competing Interests: The authors have declared that no competing interests exist.

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Citations

This article has been cited 11 times.
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