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Veterinary surgery : VS2016; 45(6); 824-830; doi: 10.1111/vsu.12520

Use of a Barbed Knotless Suture for Laparoscopic Ablation of the Nephrosplenic Space in 8 Horses.

Abstract: To evaluate use of a knotless suture for laparoscopic closure of the equine nephrosplenic space. Methods: Experimental in vivo study. Methods: Normal horses without previous history of abdominal surgery (n=8). Methods: The nephrosplenic space was closed under laparoscopic visualization using a unidirectional, barbed 0 metric absorbable suture (copolymer of glycolic acid and trimethylene carbonate). Intracorporeal suturing of the nephrosplenic space was performed in a cranial-to-caudal direction in a simple continuous fashion. Repeat evaluation was performed laparoscopically in 2 horses and by necropsy in 6 horses. The length of closure was measured and nature of the healed tissue was evaluate grossly. Results: Total surgery time was 65-167 minutes (mean ± SD, 89.6 ± 22.6). Suturing time was 30-65 minutes (40.4 ± 16.3). Second laparoscopy in 2 horses was performed at days 198 and 227. Necropsy was performed at day 69-229 postoperatively (132.7 ± 63.0) in 6 horses. The closure measured 12-14 cm in length (13 ± 1) and consisted of mature fibrous tissue bridging the splenic capsule and the nephrosplenic ligament. No residual suture material was identified grossly in any horses. The procedure was easily performed; extracorporeal suture management to hold it taut was unnecessary since the barbs had excellent purchase in the apposed tissues, and intracorporeal knot tying was not required. Conclusions: The barbed knotless suture appears to be a valid alternative to facilitate laparoscopic closure of the nephrosplenic space in normal horses; however, further work is necessary to investigate its suitability in clinically affected horses.
Publication Date: 2016-07-13 PubMed ID: 27410676DOI: 10.1111/vsu.12520Google Scholar: Lookup
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  • Journal Article

Summary

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This study evaluates the efficacy of a knotless suture used for laparoscopic closure of the nephrosplenic space in horses, considering its utility and properties like the duration of surgery, width of closure, and the tissue healing progression.

Research Methodology

  • The subject of this research were horses without any history of abdominal surgery, thereby taking out the bias of any prior wound or surgical repair interference.
  • The procedure involved laparoscopic closure of the nephrosplenic space which involves a minimal invasive surgery guided by a camera.
  • The technique specifically involved a unidirectional, barbed 0 metric absorbable suture made from the copolymer of glycolic acid and trimethylene carbonate.
  • Special mention of suturing in a cranial-to-caudal direction in a simple continuous fashion outlined the meticulously executed technique.
  • Post-surgery, reassessment was done in two horses using laparoscopy and in the remaining six through necropsy, evaluating the lengths of closure and the nature of healed tissue.

Research Findings

  • The quantified durations pointed towards a variable duration of surgery. The total surgery time varied between 65-167 minutes with an average of 89.6 minutes denoted with the standard deviation of 22.6.
  • The suturing time within these surgeries also oscillated between 30-65 minutes, with an average close to 40 minutes.
  • Post-operative checks via laparoscopy and necropsy were performed over a range of days.
  • Closure measurement showed a length of 12-14 cm.(of sutures?) This indicates the extent of the wound or injury that needed the suturing strategy in the horses.
  • Post-operative healing showed mature fibrous tissue formation as the horses recovered. This provides reassurance of the healing power of the body post the laparoscopic procedure.
  • The absence of any residual suture material in the checked horses highlighted the absorbable property of the sutures employed.

Conclusions

  • The results pointed towards the utility of barbed knotless sutures for laparoscopic closure of nephrosplenic space in horses.
  • The study provided insights into the potential usage of this type of suture but also specified the need for further research to maximise the clinical efficacy and understand its application in clinically affected horses.

Cite This Article

APA
Albanese V, Hanson RR, McMaster MA, Koehler JW, Caldwell FJ. (2016). Use of a Barbed Knotless Suture for Laparoscopic Ablation of the Nephrosplenic Space in 8 Horses. Vet Surg, 45(6), 824-830. https://doi.org/10.1111/vsu.12520

Publication

ISSN: 1532-950X
NlmUniqueID: 8113214
Country: United States
Language: English
Volume: 45
Issue: 6
Pages: 824-830

Researcher Affiliations

Albanese, Valeria
  • Department of Clinical Sciences, JT Vaughan Large Animal Teaching Hospital, Auburn, Alabama.
Hanson, R Reid
  • Department of Clinical Sciences, JT Vaughan Large Animal Teaching Hospital, Auburn, Alabama.
McMaster, Mattie A
  • Department of Clinical Sciences, JT Vaughan Large Animal Teaching Hospital, Auburn, Alabama.
Koehler, Jennifer W
  • Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama.
Caldwell, Fred J
  • Department of Clinical Sciences, JT Vaughan Large Animal Teaching Hospital, Auburn, Alabama.

MeSH Terms

  • Animals
  • Biomechanical Phenomena
  • Colonic Diseases / prevention & control
  • Colonic Diseases / surgery
  • Colonic Diseases / veterinary
  • Horse Diseases / prevention & control
  • Horse Diseases / surgery
  • Horses
  • Laparoscopy / veterinary
  • Suture Techniques / veterinary
  • Sutures / veterinary

Citations

This article has been cited 4 times.
  1. Vázquez FJ, Argüelles D, Muñoz JA, Genton M, Méndez Angulo JL, Climent F, Roquet I, Iglesias M, Velloso Álvarez A, Vitoria A, Bulnes F, Saitua A, Romero A, Ezquerra J, Prades M, López-Sanromán FJ, Rossignol F. Use of Knotless Barbed Sutures in Laparoscopic Inguinal Hernioplasty in Horses: 40 Cases. Animals (Basel) 2024 Jun 19;14(12).
    doi: 10.3390/ani14121826pubmed: 38929445google scholar: lookup
  2. Straticò P, Guerri G, Palozzo A, Varasano V, Petrizzi L. Current Use of Equine Laparoscopy in Urogenital Disorders: A Scoping Review of the Literature from 2000 to 2021. Vet Sci 2022 Jan 22;9(2).
    doi: 10.3390/vetsci9020041pubmed: 35202295google scholar: lookup
  3. Gialletti R, Nannarone S, Gandini M, Cerullo A, Bertoletti A, Scilimati N, Giusto G. Comparison of Mesh and Barbed Suture for Laparoscopic Nephrosplenic Space Ablation in Horses. Animals (Basel) 2021 Apr 12;11(4).
    doi: 10.3390/ani11041096pubmed: 33921312google scholar: lookup
  4. Adler DMT, Østergaard S, Jørgensen E, Jacobsen S. Bidirectional knotless barbed versus conventional smooth suture for closure of surgical wounds in inguinal castration in horses. BMC Vet Res 2020 Jul 17;16(1):250.
    doi: 10.1186/s12917-020-02449-6pubmed: 32680516google scholar: lookup