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Frontiers in immunology2016; 7; 392; doi: 10.3389/fimmu.2016.00392

Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFNγ-Primed Equine Mesenchymal Stem Cells.

Abstract: Mesenchymal stem cells isolated from adipose tissue (ASC) have been shown to influence the course of osteoarthritis (OA) in different animal models and are promising in veterinary medicine for horses involved in competitive sport. The aim of this study was to characterize equine ASCs (eASCs) and investigate the role of interferon-gamma (IFNγ)-priming on their therapeutic effect in a murine model of OA, which could be relevant to equine OA. Methods: ASC were isolated from subcutaneous fat. Expression of specific markers was tested by cytometry and RT-qPCR. Differentiation potential was evaluated by histology and RT-qPCR. For functional assays, naïve or IFNγ-primed eASCs were cocultured with peripheral blood mononuclear cells or articular cartilage explants. Finally, the therapeutic effect of eASCs was tested in the model of collagenase-induced OA (CIOA) in mice. Results: The immunosuppressive function of eASCs on equine T cell proliferation and their chondroprotective effect on equine cartilage explants were demonstrated . Both cartilage degradation and T cell activation were reduced by naïve and IFNγ-primed eASCs, but IFNγ-priming enhanced these functions. In CIOA, intra-articular injection of eASCs prevented articular cartilage from degradation and IFNγ-primed eASCs were more potent than naïve cells. This effect was related to the modulation of eASC secretome by IFNγ-priming. Conclusions: IFNγ-priming of eASCs potentiated their antiproliferative and chondroprotective functions. We demonstrated that the immunocompetent mouse model of CIOA was relevant to test the therapeutic efficacy of xenogeneic eASCs for OA and confirmed that IFNγ-primed eASCs may have a therapeutic value for musculoskeletal diseases in veterinary medicine.
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Publication Date: 2016-09-27 PubMed ID: 27729913PubMed Central: PMC5037129DOI: 10.3389/fimmu.2016.00392Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research demonstrates that interferon-gamma (IFNγ)-priming intensifies the therapeutic powers of equine mesenchymal stem cells in combating osteoarthritis, as observed in a mouse model. The study suggests promising implications of this regenerative therapy for the treatment of musculoskeletal diseases in horses.

Understanding the Research

The research study was outlined and conducted through a sequence of experimental setups, each uncovering valuable insights. The findings were divided into several different yet interconnected areas:

  • Cells’ Source and Functionality: Mesenchymal stem cells, particularly those derived from adipose tissue (fat), were the primary focus. This category of cells, referred to as ASCs, has shown potential in the regulation and treatment of osteoarthritis in various animal models. Specifically, the study examines equine ASCs (eASCs), given their potential usefulness in treating horses engaged in competitive sports where risk of Osteoarthritis is high.
  • Interferon-Gamma Priming: The methodology of the experiment was to observe the effects of IFNγ-priming on eASCs. By exposing the eASCs to the protein IFNγ before introducing them to the osteoarthritis environment in the mouse model, researchers aimed to analyze any potential enhanced therapeutic benefits.
  • Testing and Analysis: To effectively demonstrate their findings, the researchers isolated eASCs from subcutaneous fat, and their expression of specific markers was tested using cytometry and RT-qPCR. Additionally, naïve or IFNγ-primed eASCs were then cocultured with peripheral blood mononuclear cells or articular cartilage explants.

Findings of the Study

Valuable results arose from the study, painting a promising picture for the therapy’s potential:

  • Chondroprotective Effect: It was observed that the eASCs exhibited a chondroprotective effect. This means that they helped protect the cells of the cartilage from damage and degradation – a basic cause of osteoarthritis.
  • Immunosuppressive Function: Also seen was the immunosuppressive capacity of the eASCs on equine T cell proliferation. This implies the eASCs can suppress the immune response, potentially reducing inflammation and damage caused to joints in OA.
  • IFNγ-Primed eASCs: The IFNγ-priming of eASCs had a heightened effect on both counts, indicating the procedure could be potentially beneficial for bringing about a stronger therapeutic impact.
  • Applicability of Findings: Lastly, findings were considered promising and relevant to manage musculoskeletal diseases in real-world veterinary medicine. This was based on the use of an immunocompetent mouse model to test the therapeutic efficacy of these cells.

Cite This Article

APA
Maumus M, Roussignol G, Toupet K, Penarier G, Bentz I, Teixeira S, Oustric D, Jung M, Lepage O, Steinberg R, Jorgensen C, Noel D. (2016). Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFNγ-Primed Equine Mesenchymal Stem Cells. Front Immunol, 7, 392. https://doi.org/10.3389/fimmu.2016.00392

Publication

Frontiers in immunology
ISSN: 1664-3224
NlmUniqueID: 101560960
Country: Switzerland
Language: English
Volume: 7
Pages: 392
PII: 392

Researcher Affiliations

Maumus, Marie
  • U1183, INSERM, Hôpital Saint-Eloi, Montpellier, France; Montpellier University, UFR de Médecine, Montpellier, France.
Roussignol, Gautier
  • Sanofi , Montpellier , France.
Toupet, Karine
  • U1183, INSERM, Hôpital Saint-Eloi, Montpellier, France; Montpellier University, UFR de Médecine, Montpellier, France.
Penarier, Geraldine
  • Sanofi , Montpellier , France.
Bentz, Isabelle
  • Sanofi , Montpellier , France.
Teixeira, Sandrine
  • Sanofi , Montpellier , France.
Oustric, Didier
  • Sanofi , Montpellier , France.
Jung, Mireille
  • Sanofi , Montpellier , France.
Lepage, Olivier
  • GREMERES-ICE, University of Lyon , Marcy l'Etoile , France.
Steinberg, Regis
  • Sanofi , Montpellier , France.
Jorgensen, Christian
  • U1183, INSERM, Hôpital Saint-Eloi, Montpellier, France; Montpellier University, UFR de Médecine, Montpellier, France; Clinical Immunology and Osteoarticular Diseases Therapeutic Unit, Hôpital Lapeyronie, Montpellier, France.
Noel, Danièle
  • U1183, INSERM, Hôpital Saint-Eloi, Montpellier, France; Montpellier University, UFR de Médecine, Montpellier, France; Clinical Immunology and Osteoarticular Diseases Therapeutic Unit, Hôpital Lapeyronie, Montpellier, France.

References

This article includes 45 references
  1. Goodrich LR, Nixon AJ. Medical treatment of osteoarthritis in the horse - a review.. Vet J 2006 Jan;171(1):51-69.
    doi: 10.1016/j.tvjl.2004.07.008pubmed: 16427582google scholar: lookup
  2. Maninchedda U, Lepage OM, Gangl M, Hilairet S, Remandet B, Meot F, Penarier G, Segard E, Cortez P, Jorgensen C, Steinberg R. Development of an equine groove model to induce metacarpophalangeal osteoarthritis: a pilot study on 6 horses.. PLoS One 2015;10(2):e0115089.
    doi: 10.1371/journal.pone.0115089pmc: PMC4332493pubmed: 25680102google scholar: lookup
  3. Whitworth DJ, Banks TA. Stem cell therapies for treating osteoarthritis: prescient or premature?. Vet J 2014 Dec;202(3):416-24.
    doi: 10.1016/j.tvjl.2014.09.024pubmed: 25457267google scholar: lookup
  4. Schnabel LV, Fortier LA, McIlwraith CW, Nobert KM. Therapeutic use of stem cells in horses: which type, how, and when?. Vet J 2013 Sep;197(3):570-7.
    doi: 10.1016/j.tvjl.2013.04.018pubmed: 23778257google scholar: lookup
  5. Vidal MA, Kilroy GE, Lopez MJ, Johnson JR, Moore RM, Gimble JM. Characterization of equine adipose tissue-derived stromal cells: adipogenic and osteogenic capacity and comparison with bone marrow-derived mesenchymal stromal cells.. Vet Surg 2007 Oct;36(7):613-22.
    doi: 10.1111/j.1532-950X.2007.00313.xpubmed: 17894587google scholar: lookup
  6. Ranera B, Ordovás L, Lyahyai J, Bernal ML, Fernandes F, Remacha AR, Romero A, Vázquez FJ, Osta R, Cons C, Varona L, Zaragoza P, Martín-Burriel I, Rodellar C. Comparative study of equine bone marrow and adipose tissue-derived mesenchymal stromal cells.. Equine Vet J 2012 Jan;44(1):33-42.
    doi: 10.1111/j.2042-3306.2010.00353.xpubmed: 21668489google scholar: lookup
  7. Barberini DJ, Freitas NP, Magnoni MS, Maia L, Listoni AJ, Heckler MC, Sudano MJ, Golim MA, da Cruz Landim-Alvarenga F, Amorim RM. Equine mesenchymal stem cells from bone marrow, adipose tissue and umbilical cord: immunophenotypic characterization and differentiation potential.. Stem Cell Res Ther 2014 Feb 21;5(1):25.
    doi: 10.1186/scrt414pmc: PMC4055040pubmed: 24559797google scholar: lookup
  8. Remacha AR, Barrachina L, Álvarez-Arguedas S, Ranera B, Romero A, Vázquez FJ, Zaragoza P, Yañez R, Martín-Burriel I, Rodellar C. Expression of genes involved in immune response and in vitro immunosuppressive effect of equine MSCs.. Vet Immunol Immunopathol 2015 Jun 15;165(3-4):107-18.
    doi: 10.1016/j.vetimm.2015.04.004pubmed: 25977164google scholar: lookup
  9. Fortier LA, Potter HG, Rickey EJ, Schnabel LV, Foo LF, Chong LR, Stokol T, Cheetham J, Nixon AJ. Concentrated bone marrow aspirate improves full-thickness cartilage repair compared with microfracture in the equine model.. J Bone Joint Surg Am 2010 Aug 18;92(10):1927-37.
    doi: 10.2106/JBJS.I.01284pubmed: 20720135google scholar: lookup
  10. Carrade DD, Owens SD, Galuppo LD, Vidal MA, Ferraro GL, Librach F, Buerchler S, Friedman MS, Walker NJ, Borjesson DL. Clinicopathologic findings following intra-articular injection of autologous and allogeneic placentally derived equine mesenchymal stem cells in horses.. Cytotherapy 2011 Apr;13(4):419-30.
    doi: 10.3109/14653249.2010.536213pubmed: 21105841google scholar: lookup
  11. Broeckx S, Suls M, Beerts C, Vandenberghe A, Seys B, Wuertz-Kozak K, Duchateau L, Spaas JH. Allogenic mesenchymal stem cells as a treatment for equine degenerative joint disease: a pilot study.. Curr Stem Cell Res Ther 2014;9(6):497-503.
    doi: 10.2174/1574888X09666140826110601pubmed: 25175766google scholar: lookup
  12. Saulnier N, Viguier E, Perrier-Groult E, Chenu C, Pillet E, Roger T, Maddens S, Boulocher C. Intra-articular administration of xenogeneic neonatal Mesenchymal Stromal Cells early after meniscal injury down-regulates metalloproteinase gene expression in synovium and prevents cartilage degradation in a rabbit model of osteoarthritis.. Osteoarthritis Cartilage 2015 Jan;23(1):122-33.
    doi: 10.1016/j.joca.2014.09.007pubmed: 25219668google scholar: lookup
  13. Ferris DJ, Frisbie DD, Kisiday JD, McIlwraith CW, Hague BA, Major MD, Schneider RK, Zubrod CJ, Kawcak CE, Goodrich LR. Clinical outcome after intra-articular administration of bone marrow derived mesenchymal stem cells in 33 horses with stifle injury.. Vet Surg 2014 Mar;43(3):255-65.
    doi: 10.1111/j.1532-950X.2014.12100.xpubmed: 24433318google scholar: lookup
  14. Wilke MM, Nydam DV, Nixon AJ. Enhanced early chondrogenesis in articular defects following arthroscopic mesenchymal stem cell implantation in an equine model.. J Orthop Res 2007 Jul;25(7):913-25.
    doi: 10.1002/jor.20382pubmed: 17405160google scholar: lookup
  15. Frisbie DD, Kisiday JD, Kawcak CE, Werpy NM, McIlwraith CW. Evaluation of adipose-derived stromal vascular fraction or bone marrow-derived mesenchymal stem cells for treatment of osteoarthritis.. J Orthop Res 2009 Dec;27(12):1675-80.
    doi: 10.1002/jor.20933pubmed: 19544397google scholar: lookup
  16. McIlwraith CW, Frisbie DD, Rodkey WG, Kisiday JD, Werpy NM, Kawcak CE, Steadman JR. Evaluation of intra-articular mesenchymal stem cells to augment healing of microfractured chondral defects.. Arthroscopy 2011 Nov;27(11):1552-61.
    doi: 10.1016/j.arthro.2011.06.002pubmed: 21862278google scholar: lookup
  17. Ruiz M, Cosenza S, Maumus M, Jorgensen C, Noël D. Therapeutic application of mesenchymal stem cells in osteoarthritis.. Expert Opin Biol Ther 2016;16(1):33-42.
    doi: 10.1517/14712598.2016.1093108pubmed: 26413975google scholar: lookup
  18. Toupet K, Maumus M, Luz-Crawford P, Lombardo E, Lopez-Belmonte J, van Lent P, Garin MI, van den Berg W, Dalemans W, Jorgensen C, Noël D. Survival and biodistribution of xenogenic adipose mesenchymal stem cells is not affected by the degree of inflammation in arthritis.. PLoS One 2015;10(1):e0114962.
    doi: 10.1371/journal.pone.0114962pmc: PMC4283953pubmed: 25559623google scholar: lookup
  19. Li J, Ezzelarab MB, Cooper DK. Do mesenchymal stem cells function across species barriers? Relevance for xenotransplantation.. Xenotransplantation 2012 Sep-Oct;19(5):273-85.
    doi: 10.1111/xen.12000pmc: PMC3445044pubmed: 22978461google scholar: lookup
  20. Augello A, Tasso R, Negrini SM, Cancedda R, Pennesi G. Cell therapy using allogeneic bone marrow mesenchymal stem cells prevents tissue damage in collagen-induced arthritis.. Arthritis Rheum 2007 Apr;56(4):1175-86.
    doi: 10.1002/art.22511pubmed: 17393437google scholar: lookup
  21. Bouffi C, Bony C, Courties G, Jorgensen C, Noël D. IL-6-dependent PGE2 secretion by mesenchymal stem cells inhibits local inflammation in experimental arthritis.. PLoS One 2010 Dec 7;5(12):e14247.
    doi: 10.1371/journal.pone.0014247pmc: PMC2998425pubmed: 21151872google scholar: lookup
  22. Desando G, Cavallo C, Sartoni F, Martini L, Parrilli A, Veronesi F, Fini M, Giardino R, Facchini A, Grigolo B. Intra-articular delivery of adipose derived stromal cells attenuates osteoarthritis progression in an experimental rabbit model.. Arthritis Res Ther 2013 Jan 29;15(1):R22.
    doi: 10.1186/ar4156pmc: PMC3672720pubmed: 23360790google scholar: lookup
  23. ter Huurne M, Schelbergen R, Blattes R, Blom A, de Munter W, Grevers LC, Jeanson J, Noël D, Casteilla L, Jorgensen C, van den Berg W, van Lent PL. Antiinflammatory and chondroprotective effects of intraarticular injection of adipose-derived stem cells in experimental osteoarthritis.. Arthritis Rheum 2012 Nov;64(11):3604-13.
    doi: 10.1002/art.34626pubmed: 22961401google scholar: lookup
  24. Schelbergen RF, van Dalen S, ter Huurne M, Roth J, Vogl T, Noël D, Jorgensen C, van den Berg WB, van de Loo FA, Blom AB, van Lent PL. Treatment efficacy of adipose-derived stem cells in experimental osteoarthritis is driven by high synovial activation and reflected by S100A8/A9 serum levels.. Osteoarthritis Cartilage 2014 Aug;22(8):1158-66.
    doi: 10.1016/j.joca.2014.05.022pubmed: 24928317google scholar: lookup
  25. Manferdini C, Maumus M, Gabusi E, Piacentini A, Filardo G, Peyrafitte JA, Jorgensen C, Bourin P, Fleury-Cappellesso S, Facchini A, Noël D, Lisignoli G. Adipose-derived mesenchymal stem cells exert antiinflammatory effects on chondrocytes and synoviocytes from osteoarthritis patients through prostaglandin E2.. Arthritis Rheum 2013 May;65(5):1271-81.
    doi: 10.1002/art.37908pubmed: 23613363google scholar: lookup
  26. Maumus M, Manferdini C, Toupet K, Peyrafitte JA, Ferreira R, Facchini A, Gabusi E, Bourin P, Jorgensen C, Lisignoli G, Noël D. Adipose mesenchymal stem cells protect chondrocytes from degeneration associated with osteoarthritis.. Stem Cell Res 2013 Sep;11(2):834-44.
    doi: 10.1016/j.scr.2013.05.008pubmed: 23811540google scholar: lookup
  27. Manferdini C, Maumus M, Gabusi E, Paolella F, Grassi F, Jorgensen C, Fleury-Cappellesso S, Noël D, Lisignoli G. Lack of anti-inflammatory and anti-catabolic effects on basal inflamed osteoarthritic chondrocytes or synoviocytes by adipose stem cell-conditioned medium.. Osteoarthritis Cartilage 2015 Nov;23(11):2045-57.
    doi: 10.1016/j.joca.2015.03.025pubmed: 26521751google scholar: lookup
  28. Djouad F, Plence P, Bony C, Tropel P, Apparailly F, Sany J, Noël D, Jorgensen C. Immunosuppressive effect of mesenchymal stem cells favors tumor growth in allogeneic animals.. Blood 2003 Nov 15;102(10):3837-44.
    doi: 10.1182/blood-2003-04-1193pubmed: 12881305google scholar: lookup
  29. Kavanagh DP, Robinson J, Kalia N. Mesenchymal stem cell priming: fine-tuning adhesion and function.. Stem Cell Rev Rep 2014 Aug;10(4):587-99.
    doi: 10.1007/s12015-014-9510-7pubmed: 24752328google scholar: lookup
  30. Ren G, Zhang L, Zhao X, Xu G, Zhang Y, Roberts AI, Zhao RC, Shi Y. Mesenchymal stem cell-mediated immunosuppression occurs via concerted action of chemokines and nitric oxide.. Cell Stem Cell 2008 Feb 7;2(2):141-50.
    doi: 10.1016/j.stem.2007.11.014pubmed: 18371435google scholar: lookup
  31. Crop MJ, Baan CC, Korevaar SS, Ijzermans JN, Pescatori M, Stubbs AP, van Ijcken WF, Dahlke MH, Eggenhofer E, Weimar W, Hoogduijn MJ. Inflammatory conditions affect gene expression and function of human adipose tissue-derived mesenchymal stem cells.. Clin Exp Immunol 2010 Dec;162(3):474-86.
    doi: 10.1111/j.1365-2249.2010.04256.xpmc: PMC3026550pubmed: 20846162google scholar: lookup
  32. Pritzker KP, Gay S, Jimenez SA, Ostergaard K, Pelletier JP, Revell PA, Salter D, van den Berg WB. Osteoarthritis cartilage histopathology: grading and staging.. Osteoarthritis Cartilage 2006 Jan;14(1):13-29.
    doi: 10.1016/j.joca.2005.07.014pubmed: 16242352google scholar: lookup
  33. Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop Dj, Horwitz E. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement.. Cytotherapy 2006;8(4):315-7.
    doi: 10.1080/14653240600855905pubmed: 16923606google scholar: lookup
  34. Krampera M, Galipeau J, Shi Y, Tarte K, Sensebe L. Immunological characterization of multipotent mesenchymal stromal cells--The International Society for Cellular Therapy (ISCT) working proposal.. Cytotherapy 2013 Sep;15(9):1054-61.
    doi: 10.1016/j.jcyt.2013.02.010pubmed: 23602578google scholar: lookup
  35. Sotiropoulou PA, Perez SA, Gritzapis AD, Baxevanis CN, Papamichail M. Interactions between human mesenchymal stem cells and natural killer cells.. Stem Cells 2006 Jan;24(1):74-85.
    doi: 10.1634/stemcells.2004-0359pubmed: 16099998google scholar: lookup
  36. Ghannam S, Bouffi C, Djouad F, Jorgensen C, Noël D. Immunosuppression by mesenchymal stem cells: mechanisms and clinical applications.. Stem Cell Res Ther 2010 Mar 15;1(1):2.
    doi: 10.1186/scrt2pmc: PMC2873698pubmed: 20504283google scholar: lookup
  37. Cals FL, Hellingman CA, Koevoet W, Baatenburg de Jong RJ, van Osch GJ. Effects of transforming growth factor-β subtypes on in vitro cartilage production and mineralization of human bone marrow stromal-derived mesenchymal stem cells.. J Tissue Eng Regen Med 2012 Jan;6(1):68-76.
    doi: 10.1002/term.399pubmed: 21305699google scholar: lookup
  38. Xu C, Yu P, Han X, Du L, Gan J, Wang Y, Shi Y. TGF-β promotes immune responses in the presence of mesenchymal stem cells.. J Immunol 2014 Jan 1;192(1):103-9.
    doi: 10.4049/jimmunol.1302164pubmed: 24293629google scholar: lookup
  39. Jiang LB, Lee S, Wang Y, Xu QT, Meng DH, Zhang J. Adipose-derived stem cells induce autophagic activation and inhibit catabolic response to pro-inflammatory cytokines in rat chondrocytes.. Osteoarthritis Cartilage 2016 Jun;24(6):1071-81.
    doi: 10.1016/j.joca.2015.12.021pubmed: 26778531google scholar: lookup
  40. Caramés B, Taniguchi N, Otsuki S, Blanco FJ, Lotz M. Autophagy is a protective mechanism in normal cartilage, and its aging-related loss is linked with cell death and osteoarthritis.. Arthritis Rheum 2010 Mar;62(3):791-801.
    doi: 10.1002/art.27305pmc: PMC2838960pubmed: 20187128google scholar: lookup
  41. Tat SK, Pelletier JP, Velasco CR, Padrines M, Martel-Pelletier J. New perspective in osteoarthritis: the OPG and RANKL system as a potential therapeutic target?. Keio J Med 2009 Mar;58(1):29-40.
    doi: 10.2302/kjm.58.29pubmed: 19398882google scholar: lookup
  42. Gao F, Chiu SM, Motan DA, Zhang Z, Chen L, Ji HL, Tse HF, Fu QL, Lian Q. Mesenchymal stem cells and immunomodulation: current status and future prospects.. Cell Death Dis 2016 Jan 21;7(1):e2062.
    doi: 10.1038/cddis.2015.327pmc: PMC4816164pubmed: 26794657google scholar: lookup
  43. Wang Y, Chen X, Cao W, Shi Y. Plasticity of mesenchymal stem cells in immunomodulation: pathological and therapeutic implications.. Nat Immunol 2014 Nov;15(11):1009-16.
    doi: 10.1038/ni.3002pubmed: 25329189google scholar: lookup
  44. Polchert D, Sobinsky J, Douglas G, Kidd M, Moadsiri A, Reina E, Genrich K, Mehrotra S, Setty S, Smith B, Bartholomew A. IFN-gamma activation of mesenchymal stem cells for treatment and prevention of graft versus host disease.. Eur J Immunol 2008 Jun;38(6):1745-55.
    doi: 10.1002/eji.200738129pmc: PMC3021120pubmed: 18493986google scholar: lookup
  45. Han X, Yang Q, Lin L, Xu C, Zheng C, Chen X, Han Y, Li M, Cao W, Cao K, Chen Q, Xu G, Zhang Y, Zhang J, Schneider RJ, Qian Y, Wang Y, Brewer G, Shi Y. Interleukin-17 enhances immunosuppression by mesenchymal stem cells.. Cell Death Differ 2014 Nov;21(11):1758-68.
    doi: 10.1038/cdd.2014.85pmc: PMC4211372pubmed: 25034782google scholar: lookup

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  1. Warmink K, Rios JL, Varderidou-Minasian S, Torres-Torrillas M, van Valkengoed DR, Versteeg S, Eijkelkamp N, Weinans H, Korthagen NM, Lorenowicz MJ. Mesenchymal stem/stromal cells-derived extracellular vesicles as a potentially more beneficial therapeutic strategy than MSC-based treatment in a mild metabolic osteoarthritis model. Stem Cell Res Ther 2023 May 24;14(1):137.
    doi: 10.1186/s13287-023-03368-7pubmed: 37226203google scholar: lookup
  2. Yassin AM, AbuBakr HO, Abdelgalil AI, Farid OA, El-Behairy AM, Gouda EM. Circulating miR-146b and miR-27b are efficient biomarkers for early diagnosis of Equidae osteoarthritis. Sci Rep 2023 May 17;13(1):7966.
    doi: 10.1038/s41598-023-35207-3pubmed: 37198318google scholar: lookup
  3. Jammes M, Contentin R, Cassé F, Galéra P. Equine osteoarthritis: Strategies to enhance mesenchymal stromal cell-based acellular therapies. Front Vet Sci 2023;10:1115774.
    doi: 10.3389/fvets.2023.1115774pubmed: 36846261google scholar: lookup
  4. Boffa A, Perucca Orfei C, Sourugeon Y, Laver L, Magalon J, Sánchez M, Tischer T, de Girolamo L, Filardo G. Cell-based therapies have disease-modifying effects on osteoarthritis in animal models. A systematic review by the ESSKA Orthobiologic Initiative. Part 2: bone marrow-derived cell-based injectable therapies. Knee Surg Sports Traumatol Arthrosc 2023 Aug;31(8):3230-3242.
    doi: 10.1007/s00167-023-07320-3pubmed: 36823238google scholar: lookup
  5. Perucca Orfei C, Boffa A, Sourugeon Y, Laver L, Magalon J, Sánchez M, Tischer T, Filardo G, de Girolamo L. Cell-based therapies have disease-modifying effects on osteoarthritis in animal models. A systematic review by the ESSKA Orthobiologic Initiative. Part 1: adipose tissue-derived cell-based injectable therapies. Knee Surg Sports Traumatol Arthrosc 2023 Feb;31(2):641-655.
    doi: 10.1007/s00167-022-07063-7pubmed: 36104484google scholar: lookup
  6. Yan B, Lv S, Tong P, Yan L, Chen Z, Zhou L, Yuan Q, Guo L, Shan L. Intra-Articular Injection of Adipose-Derived Stem Cells Ameliorates Pain and Cartilage Anabolism/Catabolism in Osteoarthritis: Preclinical and Clinical Evidences. Front Pharmacol 2022;13:854025.
    doi: 10.3389/fphar.2022.854025pubmed: 35387326google scholar: lookup
  7. Kay AG, Treadwell K, Roach P, Morgan R, Lodge R, Hyland M, Piccinini AM, Forsyth NR, Kehoe O. Therapeutic Effects of Hypoxic and Pro-Inflammatory Priming of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Inflammatory Arthritis. Int J Mol Sci 2021 Dec 23;23(1).
    doi: 10.3390/ijms23010126pubmed: 35008555google scholar: lookup
  8. Di Francesco P, Cajon P, Desterke C, Perron Lepage MF, Lataillade JJ, Kadri T, Lepage OM. Effect of Allogeneic Oral Mucosa Mesenchymal Stromal Cells on Equine Wound Repair. Vet Med Int 2021;2021:5024905.
    doi: 10.1155/2021/5024905pubmed: 34950446google scholar: lookup
  9. Chen T, Weng W, Liu Y, Aspera-Werz RH, Nüssler AK, Xu J. Update on Novel Non-Operative Treatment for Osteoarthritis: Current Status and Future Trends. Front Pharmacol 2021;12:755230.
    doi: 10.3389/fphar.2021.755230pubmed: 34603064google scholar: lookup
  10. Maleitzke T, Elazaly H, Festbaum C, Eder C, Karczewski D, Perka C, Duda GN, Winkler T. Mesenchymal Stromal Cell-Based Therapy-An Alternative to Arthroplasty for the Treatment of Osteoarthritis? A State of the Art Review of Clinical Trials. J Clin Med 2020 Jun 30;9(7).
    doi: 10.3390/jcm9072062pubmed: 32630066google scholar: lookup
  11. Hong JI, Park IY, Kim HA. Understanding the Molecular Mechanisms Underlying the Pathogenesis of Arthritis Pain Using Animal Models. Int J Mol Sci 2020 Jan 14;21(2).
    doi: 10.3390/ijms21020533pubmed: 31947680google scholar: lookup
  12. Daems R, Van Hecke L, Schwarzkopf I, Depuydt E, Broeckx SY, David M, Beerts C, Vandekerckhove P, Spaas JH. A Feasibility Study on the Use of Equine Chondrogenic Induced Mesenchymal Stem Cells as a Treatment for Natural Occurring Osteoarthritis in Dogs. Stem Cells Int 2019;2019:4587594.
    doi: 10.1155/2019/4587594pubmed: 31281373google scholar: lookup
  13. Ragni E, De Luca P, Perucca Orfei C, Colombini A, Viganò M, Lugano G, Bollati V, de Girolamo L. Insights into Inflammatory Priming of Adipose-Derived Mesenchymal Stem Cells: Validation of Extracellular Vesicles-Embedded miRNA Reference Genes as A Crucial Step for Donor Selection. Cells 2019 Apr 23;8(4).
    doi: 10.3390/cells8040369pubmed: 31018576google scholar: lookup
  14. Huang H, Zhang ZF, Qin FW, Tang W, Liu DH, Wu PY, Jiao F. Icariin inhibits chondrocyte apoptosis and angiogenesis by regulating the TDP-43 signaling pathway. Mol Genet Genomic Med 2019 Apr;7(4):e00586.
    doi: 10.1002/mgg3.586pubmed: 30734541google scholar: lookup
  15. Barrachina L, Remacha AR, Romero A, Vitoria A, Albareda J, Prades M, Roca M, Zaragoza P, Vázquez FJ, Rodellar C. Assessment of effectiveness and safety of repeat administration of proinflammatory primed allogeneic mesenchymal stem cells in an equine model of chemically induced osteoarthritis. BMC Vet Res 2018 Aug 17;14(1):241.
    doi: 10.1186/s12917-018-1556-3pubmed: 30119668google scholar: lookup
  16. Damia E, Chicharro D, Lopez S, Cuervo B, Rubio M, Sopena JJ, Vilar JM, Carrillo JM. Adipose-Derived Mesenchymal Stem Cells: Are They a Good Therapeutic Strategy for Osteoarthritis?. Int J Mol Sci 2018 Jun 30;19(7).
    doi: 10.3390/ijms19071926pubmed: 29966351google scholar: lookup
  17. Cosenza S, Toupet K, Maumus M, Luz-Crawford P, Blanc-Brude O, Jorgensen C, Noël D. Mesenchymal stem cells-derived exosomes are more immunosuppressive than microparticles in inflammatory arthritis. Theranostics 2018;8(5):1399-1410.
    doi: 10.7150/thno.21072pubmed: 29507629google scholar: lookup
  18. Cosenza S, Ruiz M, Toupet K, Jorgensen C, Noël D. Mesenchymal stem cells derived exosomes and microparticles protect cartilage and bone from degradation in osteoarthritis. Sci Rep 2017 Nov 24;7(1):16214.
    doi: 10.1038/s41598-017-15376-8pubmed: 29176667google scholar: lookup
  19. Zhang L, Yu Z, Liu S, Liu F, Zhou S, Zhang Y, Tian Y. Advanced progress of adipose-derived stem cells-related biomaterials in maxillofacial regeneration. Stem Cell Res Ther 2025 Mar 5;16(1):110.
    doi: 10.1186/s13287-025-04191-ypubmed: 40038758google scholar: lookup
  20. Horie T, Hirata H, Sakamoto T, Kitajima H, Fuku A, Nakamura Y, Sunatani Y, Tanida I, Sunami H, Tachi Y, Ishigaki Y, Yamamoto N, Shimizu Y, Ichiseki T, Kaneuji A, Iwabuchi K, Osawa S, Kawahara N. Multiomics analyses reveal adipose-derived stem cells inhibit the inflammatory response of M1-like macrophages through secreting lactate. Stem Cell Res Ther 2024 Dec 18;15(1):485.
    doi: 10.1186/s13287-024-04072-wpubmed: 39696485google scholar: lookup
  21. Boulestreau J, Maumus M, Bertolino Minani G, Jorgensen C, Noël D. Anti-aging effect of extracellular vesicles from mesenchymal stromal cells on senescence-induced chondrocytes in osteoarthritis. Aging (Albany NY) 2024 Nov 22;16(21):13252-13270.
    doi: 10.18632/aging.206158pubmed: 39578049google scholar: lookup
  22. Boulestreau J, Maumus M, Bertolino GM, Toupet K, Jorgensen C, Noël D. Extracellular vesicles from senescent mesenchymal stromal cells are defective and cannot prevent osteoarthritis. J Nanobiotechnology 2024 May 16;22(1):255.
    doi: 10.1186/s12951-024-02509-1pubmed: 38755672google scholar: lookup
  23. Velot É, Balmayor ER, Bertoni L, Chubinskaya S, Cicuttini F, de Girolamo L, Demoor M, Grigolo B, Jones E, Kon E, Lisignoli G, Murphy M, Noël D, Vinatier C, van Osch GJVM, Cucchiarini M. Women's contribution to stem cell research for osteoarthritis: an opinion paper. Front Cell Dev Biol 2023;11:1209047.
    doi: 10.3389/fcell.2023.1209047pubmed: 38174070google scholar: lookup
  24. Ferreira-Baptista C, Ferreira R, Fernandes MH, Gomes PS, Colaço B. Influence of the Anatomical Site on Adipose Tissue-Derived Stromal Cells' Biological Profile and Osteogenic Potential in Companion Animals. Vet Sci 2023 Nov 24;10(12).
    doi: 10.3390/vetsci10120673pubmed: 38133224google scholar: lookup
  25. Jammes M, Contentin R, Audigié F, Cassé F, Galéra P. Effect of pro-inflammatory cytokine priming and storage temperature of the mesenchymal stromal cell (MSC) secretome on equine articular chondrocytes. Front Bioeng Biotechnol 2023;11:1204737.
    doi: 10.3389/fbioe.2023.1204737pubmed: 37720315google scholar: lookup
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