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Home / Equine Research Bank / Vet Clin Pathol / Validation of a novel saliva-based ELISA test for diagnosing...
Veterinary clinical pathology2016; 45(2); 335-346; doi: 10.1111/vcp.12364

Validation of a novel saliva-based ELISA test for diagnosing tapeworm burden in horses.

Abstract: Tapeworm infections pose a significant threat to equine health as they are associated with clinical cases of colic. Diagnosis of tapeworm burden using fecal egg counts (FECs) is unreliable, and, although a commercial serologic ELISA for anti-tapeworm antibodies is available, it requires a veterinarian to collect the blood sample. A reliable diagnostic test using an owner-accessible sample such as saliva could provide a cost-effective alternative for tapeworm testing in horses, and allow targeted deworming strategies. Objective: The purpose of the study was to statistically validate a saliva tapeworm ELISA test and compare to a tapeworm-specific IgG(T) serologic ELISA. Methods: Serum samples (139) and matched saliva samples (104) were collected from horses at a UK abattoir. The ileocecal junction and cecum were visually examined for tapeworms and any present were counted. Samples were analyzed using a serologic ELISA and the saliva tapeworm test. The test results were compared to tapeworm numbers and the various data sets were statistically analyzed. Results: Saliva scores had strong positive correlations with both infection intensity (0.74) and serologic results (Spearman's rank coefficients; 0.74 and 0.86, respectively). The saliva tapeworm test was capable of identifying the presence of one or more tapeworms with 83% sensitivity and 85% specificity. Importantly, no high-burden (more than 20 tapeworms) horses were misdiagnosed. Conclusions: The saliva tapeworm test has statistical accuracy for detecting tapeworm burdens in horses with 83% sensitivity and 85% specificity, similar to those of the serologic ELISA (85% and 78%, respectively).
© 2016 American Society for Veterinary Clinical Pathology.
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Publication Date: 2016-05-24 PubMed ID: 27218436DOI: 10.1111/vcp.12364Google Scholar: Lookup
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  • Journal Article
  • Validation Study

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article presents the introduction and validation of a new saliva-based test for determining the level of tapeworm infection in horses. This novel diagnostic test could allow a cost-effective and accessible way to identify tapeworms, and thus curb related health risks in equines.

Objective of the Study

  • The main goal of this project was to evaluate and authenticate a saliva-based ELISA (enzyme-linked immunosorbent assay) test for diagnosing and quantifying tapeworm infections in horses.
  • At the same time, the results from this newly developed diagnostic tool were contrasted to those acquired from a tapeworm-specific IgG(T) serologic ELISA, a method currently in use but it needs a professional to collect horse’s blood.

Methodology

  • The researchers collected serum samples from 139 horses at a UK-based abattoir, along with saliva samples from 104 of those horses.
  • For each of these equines, the area at the junction of the ileum and cecum was visually inspected to identify and count any tapeworms.
  • Each sample was then tested using both the established serologic ELISA and the newly proposed saliva tapeworm test, with the results compared to the physical count of tapeworms.
  • The collected data was further subjected to in-depth statistical analyses for a reliable comparison of the two assessment methods.

Results

  • The saliva test’s reported results align strongly with both the infection severity and the serologic ELISA results. The correlation coefficients were found to be 0.74 and 0.86, respectively.
  • Significantly, the saliva tapeworm test could reliably identify the presence of one or more tapeworms, with an accuracy of 83% and a specificity of 85%.
  • A crucial finding was that no high-burden horses (i.e., horses with more than 20 tapeworms) were misdiagnosed using the saliva test.

Conclusions

  • Ultimately, the saliva tapeworm test was found to be statistically accurate, with an effectiveness and precision nearly on par with the existing serologic ELISA – 83% versus 85% respectively for sensitivity, and 85% versus 78% respectively for specificity.
  • The success of this research would suggest that the saliva-based ELISA test offers a practical, efficient, and cost-effective approach for diagnosing tapeworm infections in horses, thereby facilitating better-targeted anti-tapeworm strategies.

Cite This Article

APA
Lightbody KL, Davis PJ, Austin CJ. (2016). Validation of a novel saliva-based ELISA test for diagnosing tapeworm burden in horses. Vet Clin Pathol, 45(2), 335-346. https://doi.org/10.1111/vcp.12364

Publication

Veterinary clinical pathology
ISSN: 1939-165X
NlmUniqueID: 9880575
Country: United States
Language: English
Volume: 45
Issue: 2
Pages: 335-346

Researcher Affiliations

Lightbody, Kirsty L
  • Austin Davis Biologics, Great Addington, UK.
Davis, Paul J
  • Austin Davis Biologics, Great Addington, UK.
Austin, Corrine J
  • Austin Davis Biologics, Great Addington, UK.

MeSH Terms

  • Animals
  • Antibodies, Helminth / analysis
  • Cestoda / immunology
  • Cestode Infections / diagnosis
  • Cestode Infections / veterinary
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • Horse Diseases / diagnosis
  • Horse Diseases / parasitology
  • Horses
  • Immunoglobulin G / analysis
  • Immunoglobulin G / immunology
  • Saliva / immunology

Citations

This article has been cited 15 times.
  1. Abbas G, Nielsen MK, E-Hage C, Ghafar A, Beveridge I, Bauquier J, Beasley A, Wilkes EJA, Carrigan P, Cudmore L, Jacobson C, Hughes KJ, Jabbar A. Recent advances in intestinal helminth parasites of horses in the Asia-Pacific region: Current trends, challenges and future directions. Int J Parasitol Drugs Drug Resist 2025 Dec;29:100622.
    doi: 10.1016/j.ijpddr.2025.100622pubmed: 41135277google scholar: lookup
  2. Wang T, Chen X, Yan X, Su Y, Gao W, Liu C, Wang W. Progress in serology and molecular biology of equine parasite diagnosis: sustainable control strategies. Front Vet Sci 2025;12:1663577.
    doi: 10.3389/fvets.2025.1663577pubmed: 40979365google scholar: lookup
  3. Kukurić T, Erdeljan M, Matthews JB, Lightbody KL, Austin CJ, Peczak N, Uzelac A, Klun I, Simin S. A Prevalence Study on Anoplocephala spp. in Serbian Horses: Navigating Diagnostic Challenges and Understanding Infection Risks. Animals (Basel) 2025 Jul 16;15(14).
    doi: 10.3390/ani15142094pubmed: 40723557google scholar: lookup
  4. Arshad A, Reif AH, Cavalleri JV, Desvars-Larrive A. Zoonotic pathogens in equids in Central Europe: a systematic review. BMC Vet Res 2025 Jul 8;21(1):451.
    doi: 10.1186/s12917-025-04915-5pubmed: 40629389google scholar: lookup
  5. Nielsen MK, Pyatt A, Perrett J, Tydén E, van Doorn D, Pihl TH, Schmidt JS, von Samson-Himmelstjerna G, Beasley A, Abbas G, Jabbar A. Global equine parasite control guidelines: Consensus or confusion?. Int J Parasitol Drugs Drug Resist 2025 Aug;28:100600.
    doi: 10.1016/j.ijpddr.2025.100600pubmed: 40472642google scholar: lookup
  6. Northcote HM, Wititkornkul B, Cutress DJ, Allen ND, Brophy PM, Wonfor RE, Morphew RM. A dominance of Mu class glutathione transferases within the equine tapeworm Anoplocephala perfoliata. Parasitology 2024 Mar;151(3):282-294.
    doi: 10.1017/S0031182024000015pubmed: 38200699google scholar: lookup
  7. Matthews JB, Peczak N, Lightbody KL. The Use of Innovative Diagnostics to Inform Sustainable Control of Equine Helminth Infections. Pathogens 2023 Oct 11;12(10).
    doi: 10.3390/pathogens12101233pubmed: 37887749google scholar: lookup
  8. Burcáková L, Königová A, Kuzmina TA, Austin CJ, Matthews JB, Lightbody KL, Peczak NA, Syrota Y, Várady M. Equine tapeworm (Anoplocephala spp.) infection: evaluation of saliva- and serum-based antibody detection methods and risk factor analysis in Slovak horse populations. Parasitol Res 2023 Dec;122(12):3037-3052.
    doi: 10.1007/s00436-023-07994-1pubmed: 37803152google scholar: lookup
  9. Athanasiou LV, Katsogiannou EG, Tyrnenopoulou P, Gougoulis D, Apostolidis KN, Papadakis SM, Kokkinaki KCG, Papatsiros VG, Tsokana CN. Evidence of Horse Exposure to Anaplasma phagocytophilum, Borrelia burgdorferi, and Leishmania infantum in Greece through the Detection of IgG Antibodies in Serum and in an Alternative Diagnostic Sample-The Saliva. Biomolecules 2023 Sep 11;13(9).
    doi: 10.3390/biom13091374pubmed: 37759774google scholar: lookup
  10. Buono F, Veneziano V, Veronesi F, Molento MB. Horse and donkey parasitology: differences and analogies for a correct diagnostic and management of major helminth infections. Parasitology 2023 Oct;150(12):1119-1138.
    doi: 10.1017/S0031182023000525pubmed: 37221816google scholar: lookup
  11. Hautala K, Pursiainen J, Näreaho A, Nyman T, Varmanen P, Sukura A, Nielsen MK, Savijoki K. Label-free quantitative proteomics and immunoblotting identifies immunoreactive and other excretory-secretory (E/S) proteins of Anoplocephala perfoliata. Front Immunol 2022;13:1045468.
    doi: 10.3389/fimmu.2022.1045468pubmed: 36466892google scholar: lookup
  12. Pawlas-Opiela M, Jawor P, Galli J, Zak-Bochenek A, Gorczykowski M, Galli J, Sołtysiak Z, Stefaniak T. The relationship between the intensity of Gasterophilus intestinalis larvae infection and the serum and salivary humoral immune response in horses. Sci Rep 2022 Oct 20;12(1):17573.
    doi: 10.1038/s41598-022-21482-zpubmed: 36266409google scholar: lookup
  13. Slater R, Frau A, Hodgkinson J, Archer D, Probert C. A Comparison of the Colonic Microbiome and Volatile Organic Compound Metabolome of Anoplocephala perfoliata Infected and Non-Infected Horses: A Pilot Study. Animals (Basel) 2021 Mar 9;11(3).
    doi: 10.3390/ani11030755pubmed: 33803473google scholar: lookup
  14. Gehlen H, Wulke N, Ertelt A, Nielsen MK, Morelli S, Traversa D, Merle R, Wilson D, Samson-Himmelstjerna GV. Comparative Analysis of Intestinal Helminth Infections in Colic and Non-Colic Control Equine Patients. Animals (Basel) 2020 Oct 19;10(10).
    doi: 10.3390/ani10101916pubmed: 33086590google scholar: lookup
  15. Jürgenschellert L, Krücken J, Austin CJ, Lightbody KL, Bousquet E, von Samson-Himmelstjerna G. Investigations on the occurrence of tapeworm infections in German horse populations with comparison of different antibody detection methods based on saliva and serum samples. Parasit Vectors 2020 Sep 10;13(1):462.
    doi: 10.1186/s13071-020-04318-5pubmed: 32912340google scholar: lookup
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