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Home / Equine Research Bank / BMC Vet Res / Validation of an equine serum amyloid A assay with an unusua...
BMC veterinary research2019; 15(1); 462; doi: 10.1186/s12917-019-2211-3

Validation of an equine serum amyloid A assay with an unusually broad working range.

Abstract: Serum amyloid A (SAA) is a major equine acute phase protein and of great value in detection and monitoring of inflammation. A new immunoturbidometric assay based on monoclonal antibodies (VET-SAA, Eiken Chemical Co., Japan) may be useful for SAA measurements in routine diagnostic laboratories. The aim of the study was to validate the VET-SAA immunoturbidometric assay and use it to measure serum SAA concentrations in a variety of clinical cases. Precision was assessed by intra- and interassay coefficients of variation of repeated measurements of serum pools (low, intermediate, high concentrations of SAA). Accuracy was estimated by linearity under dilution. Detection limit was determined by replicate determinations of ionized water. Measurements were compared to measurements performed in a previously validated SAA assay (LZSAA assay, Eiken Chemical Co., Japan). Subsequently, the VET-SAA assay was used for measuring serum SAA concentrations in horses with and without inflammation. Results: Detection limit was 1.2 mg/L. Without modifications, the assay measured SAA concentrations with acceptable reliability in a broad concentration range (0 to > 6000 mg/L). In the 0-3000 mg/L range, the assay demonstrated good precision and accuracy, and concentrations correlated well with those obtained in the LZSAA assay, albeit with a slight systematic bias. Concentrations of SAA assessed in horses with and without inflammation followed the expected pattern, with significantly higher concentrations in horses with systemic inflammation than in healthy horses and horses with non-inflammatory disease. Conclusions: The assay was unique in its ability to measure SAA concentrations with acceptable reliability over an extreme concentration range. This is relevant in the equine species, where SAA concentrations may reach very high concentrations.
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Publication Date: 2019-12-19 PubMed ID: 31856804PubMed Central: PMC6923866DOI: 10.1186/s12917-019-2211-3Google Scholar: Lookup
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  • Journal Article
  • Validation Study

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study validates the VET-SAA immunoturbidometric assay for measuring equine Serum Amyloid A, a protein involved in detecting and monitoring inflammation in horses. The authors found that this assay generates reliable results over an unusual concentration range.

Overview of Research Objectives

  • The study aimed to evaluate the effectiveness of a new immunoturbidometric assay (VET-SAA) in quantifying levels of Serum Amyloid A (SAA) protein in horses.
  • SAA is essential for identifying and monitoring inflammation in horses.
  • The researchers sought to validate the reliability, precision, and accuracy of the VET-SAA assay across a unique concentration range.
  • They also wanted to test how the VET-SAA assay results correspond to results from an established SAA assay (LZSAA).

Research Methodology and Results

  • Precision was measured using intra- and interassay coefficients variation of repeated measurements, obtained from serum pools with different SAA concentrations.
  • Accuracy was checked by assessing the linearity under dilution. The detection limit was determined by making multiple ionized water measurements.
  • The results of the VET-SAA assay were compared with those obtained from the LZSAA assay.
  • Subsequently, they performed the VET-SAA assay on horses with and without inflammation.
  • The detection limit of the assay was 1.2 mg/L. The researchers found the assay provided reliable SAA measurements in a broad concentration range, without requiring any modifications.
  • With a slightly systematic bias, the assay presented good precision and accuracy for ranges between 0-3000 mg/L and correlated well with LZSAA assay results.
  • Finally, SAA concentrations were higher in horses with systemic inflammation than in healthy horses or horses with non-inflammatory diseases, which complied with anticipated trends.

Study Conclusions

  • The researchers concluded that the VET-SAA immunoturbidometric assay is unique in its ability to measure SAA concentrations reliably across an extremely broad concentration range.
  • This capacity is significant because SAA levels in horses can reach very high values, making this assay highly beneficial for diagnosing and tracking inflammation in equine species.

Cite This Article

APA
Jacobsen S, Vinther AM, Kjelgaard-Hansen M, Nielsen LN. (2019). Validation of an equine serum amyloid A assay with an unusually broad working range. BMC Vet Res, 15(1), 462. https://doi.org/10.1186/s12917-019-2211-3

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 15
Issue: 1
Pages: 462
PII: 462

Researcher Affiliations

Jacobsen, Stine
  • Department of Veterinary Clinical Sciences, Section of Medicine and Surgery, University of Copenhagen, Agrovej 8, Taastrup, Denmark. stj@sund.ku.dk.
Vinther, Anne Mette
  • Department of Veterinary Clinical Sciences, Section of Medicine and Surgery, University of Copenhagen, Agrovej 8, Taastrup, Denmark.
Kjelgaard-Hansen, Mads
  • Ascendis Pharma A/S, Tuborg Boulevard 12, Hellerup, Denmark.
  • Department of Veterinary Clinical Sciences, The Veterinary Diagnostic Laboratory, University of Copenhagen, Dyrlaegevej 46, Frederiksberg C, Denmark.
Nielsen, Lise Nikolic
  • Department of Veterinary Clinical Sciences, The Veterinary Diagnostic Laboratory, University of Copenhagen, Dyrlaegevej 46, Frederiksberg C, Denmark.

MeSH Terms

  • Acute-Phase Proteins
  • Animals
  • Female
  • Horse Diseases / blood
  • Horses / blood
  • Immunoturbidimetry / methods
  • Immunoturbidimetry / veterinary
  • Inflammation / blood
  • Inflammation / diagnosis
  • Inflammation / veterinary
  • Limit of Detection
  • Male
  • Reproducibility of Results
  • Serum Amyloid A Protein / analysis

Grant Funding

  • N/A / Eiken Chemical Company

Conflict of Interest Statement

The authors declare that they have no competing interests.

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Citations

This article has been cited 9 times.
  1. Waugh EM, Haining H, Harvie J, Ridyard AE, Eckersall PD. Validation of an automated immunoturbidimetric assay for feline serum amyloid A. BMC Vet Res 2022 Sep 28;18(1):359.
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  2. Behling-Kelly E, Haak CE, Carney P, Waffle J, Eaton K, Goggs R. Acute phase protein response and changes in lipoprotein particle size in dogs with systemic inflammatory response syndrome. J Vet Intern Med 2022 May;36(3):993-1004.
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  9. Hazuchova K, Held S, Klemm I, Bauer N. Simplified Light's Criteria and Acute Phase Proteins Reflect Aetiology of Feline Body Cavity Effusions Better than the Traditional Classification Scheme. Animals (Basel) 2023 Jun 8;13(12).
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