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Home / Equine Research Bank / J Vet Intern Med / Value of measuring markers of lipid metabolism in horses dur...
Journal of veterinary internal medicine2024; doi: 10.1111/jvim.17199

Value of measuring markers of lipid metabolism in horses during an oral glucose test.

Abstract: Characterizing the lipid response to an oral glucose test (OGT) might improve our understanding of Equine Metabolic Syndrome. Objective: To describe the effects of an OGT on lipid metabolism and determine the value of measuring triglyceride and nonesterified fatty acid (NEFA) concentrations in hyperinsulinemic (HI) and insulin-resistant (IR) horses. Methods: Twenty horses including 7 HI-IR horses, 4 HI-non-IR horses, and 9 non-HI-non-IR horses (control). Methods: Cross-sectional design. Horses underwent an OGT, with blood samples collected at 0, 60, 90, and 120 minutes. Insulin, glucose, triglyceride, and NEFA concentrations were measured and compared over time and between groups, with P < .05 considered significant. Results: In all horses, the OGT had a significant effect on triglyceride concentrations (median [interquartile range]: .35 [.30-.50] mmol/L at 0 minute vs .25 [.21-.37] mmol/L at 120 minutes, P = .005) and on NEFA concentrations (.1 [.1-.2] mEq/L at 0 minute vs .05 [.05-.1] mEq/L at 120 minutes, P = .0009). However, horses with HI and IR had higher triglyceride areas under the curve (AUC, 79.46 ± 46.59 vs 33.32 ± 6.75 mmol/L*min, P = .01) as well as NEFA AUC (9.1 ± 2.9 vs 6.0 ± 6.8 mEq/L*min, P = .03) than control horses. No significant difference was detected between control and HI non-IR horses. Conclusions: Determining triglyceride and NEFA concentrations might help assess tissue insulin resistance during an OGT.
© 2024 The Author(s). Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
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Publication Date: 2024-09-18 PubMed ID: 39291576DOI: 10.1111/jvim.17199Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research seeks to understand the effect of an oral glucose test (OGT) on the lipid metabolism of horses, particularly those with hyperinsulinemia (HI) and insulin resistance (IR), by measuring triglyceride and nonesterified fatty acid (NEFA) concentrations. The results indicate altered lipid metabolism in HI and IR horses which could assist in assessing tissue insulin resistance.

Research Objective

  • The main goal of this study was to unravel the impact of an oral glucose test (OGT) on lipid metabolism in horses. The study was designed specifically to establish the significance of measuring triglyceride and nonesterified fatty acid (NEFA) concentrations in hyperinsulinemic (HI) and insulin-resistant (IR) horses.

Research Methods

  • Twenty horses participated in the study, among them 7 were HI-IR, 4 were HI-non-IR, and 9 were non-HI-non-IR horses, acting as the control group.
  • The researchers utilized a cross-sectional design in their approach and carried out an Oral Glucose Test (OGT).
  • Blood samples were collected at four different intervals: 0, 60, 90, and 120 minutes during the test.
  • The researchers assessed Insulin, glucose, triglyceride, and NEFA concentrations and compared these results between the different time intervals and groups, considering a P-value of less than .05 as statistically significant.

Research Results

  • The OGT significantly affected both triglyceride and NEFA concentrations in all the horses. Triglyceride levels moderately decreased, and NEFA concentrations fell noticeably over time.
  • However, horses suffering from HI and IR displayed higher triglyceride and NEFA areas under the curve (AUC) when compared to the control group.
  • No notable difference was found between the control group and the HI-non-IR horses.

Research Conclusion

  • These findings suggest that measuring triglyceride and NEFA concentrations during an OGT can be valuable in evaluating tissue insulin resistance in horses.
  • This study adds to the growing body of research on Equine Metabolic Syndrome, particularly the role of glucose and lipid metabolism.

Cite This Article

APA
Zemek CHK, Kemp KL, Bertin FR. (2024). Value of measuring markers of lipid metabolism in horses during an oral glucose test. J Vet Intern Med. https://doi.org/10.1111/jvim.17199

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English

Researcher Affiliations

Zemek, Claire H K
  • School of Veterinary Science, The University of Queensland, Gatton, Queensland, Australia.
Kemp, Kate L
  • School of Veterinary Science, The University of Queensland, Gatton, Queensland, Australia.
Bertin, François-René
  • School of Veterinary Science, The University of Queensland, Gatton, Queensland, Australia.
  • College of Veterinary Medicine, Purdue University, West-Lafayette, Indiana, USA.

Grant Funding

  • D19-EQ-302 / Morris Animal Foundation
  • VETS5017 / The University of Queensland School of Veterinary Science

References

This article includes 42 references
  1. Frank N, Tadros EM. Insulin dysregulation.. Equine Vet J 2014;46:103‐112.
  2. de Laat MA, McGowan CM, Sillence MN. Equine laminitis: induced by 48 h hyperinsulinaemia in Standardbred horses.. Equine Vet J 2010;42:129‐135.
  3. Meier AD, de Laat MA, Reiche DB. The oral glucose test predicts laminitis risk in ponies fed a diet high in nonstructural carbohydrates.. Domest Anim Endocrinol 2018;63:1‐9.
  4. Pratt‐Phillips SE, Geor RJ, McCutcheon LJ. Comparison among the euglycemic‐hyperinsulinemic clamp, insulin‐modified frequently sampled intravenous glucose tolerance test, and oral glucose tolerance test for assessment of insulin sensitivity in healthy Standardbreds.. Am J Vet Res 2015;76:84‐91.
  5. Bertin FR, Sojka‐Kritchevsky JE. Comparison of a 2‐step insulin‐response test to conventional insulin‐sensitivity testing in horses.. Domest Anim Endocrinol 2013;44:19‐25.
  6. Kojta I, Chacinska M, Blachnio‐Zabielska A. Obesity, bioactive lipids, and adipose tissue inflammation in insulin resistance.. Nutrients 2020;12(5):1305.
  7. Blaue D, Schedlbauer C, Starzonek J. Effects of body weight gain on insulin and lipid metabolism in equines.. Domest Anim Endocrinol 2019;68:111‐118.
  8. Henneke DR, Potter GD, Kreider JL. Relationship between condition score, physical measurements and body fat percentage in mares.. Equine Vet J 1983;15:371‐372.
  9. Carter RA, Geor RJ, Burton Staniar W, Cubitt TA, Harris PA. Apparent adiposity assessed by standardised scoring systems and morphometric measurements in horses and ponies.. Vet J 2009;179:204‐210.
  10. Frank N, Elliott SB, Brandt LE, Keisler DH. Physical characteristics, blood hormone concentrations, and plasma lipid concentrations in obese horses with insulin resistance.. J Am Vet Med Assoc 2006;228:1383‐1390.
  11. Pleasant RS, Suagee JK, Thatcher CD, Elvinger F, Geor RJ. Adiposity, plasma insulin, leptin, lipids, and oxidative stress in mature light breed horses.. J Vet Intern Med 2013;27:576‐582.
  12. Durham AE, Frank N, McGowan CM. ECEIM consensus statement on equine metabolic syndrome.. J Vet Intern Med 2019;33:335‐349.
  13. Bamford NJ, Potter SJ, Harris PA, Bailey SR. Effect of increased adiposity on insulin sensitivity and adipokine concentrations in horses and ponies fed a high fat diet, with or without a once daily high glycaemic meal.. Equine Vet J 2016;48:368‐373.
  14. Suagee JK, Corl BA, Hulver MW, McCutcheon LJ, Geor RJ. Effects of hyperinsulinemia on glucose and lipid transporter expression in insulin‐sensitive horses.. Domest Anim Endocrinol 2011;40:173‐181.
  15. Watson TD, Burns L, Packard CJ. Effects of pregnancy and lactation on plasma lipid and lipoprotein concentrations, lipoprotein composition and post‐heparin lipase activities in Shetland pony mares.. J Reprod Fertil 1993;97:563‐568.
  16. Carter RA, McCutcheon LJ, George LA. Effects of diet‐induced weight gain on insulin sensitivity and plasma hormone and lipid concentrations in horses.. Am J Vet Res 2009;70:1250‐1258.
  17. Morgan RA, McGowan TW, McGowan CM. Prevalence and risk factors for hyperinsulinaemia in ponies in Queensland, Australia.. Aust Vet J 2014;92:101‐106.
  18. Knowles EJ, Harris PA, Elliott J, Chang YM, Menzies‐Gow NJ. Factors associated with insulin responses to oral sugars in a mixed‐breed cohort of ponies.. Equine Vet J 2024;56:253‐263.
  19. Knowles EJ, Elliott J, Harris PA, Chang YM, Menzies‐Gow NJ. Predictors of laminitis development in a cohort of nonlaminitic ponies.. Equine Vet J 2023;55:12‐23.
  20. Menzies‐Gow NJ, Harris PA, Elliott J. Prospective cohort study evaluating risk factors for the development of pasture‐associated laminitis in the United Kingdom.. Equine Vet J 2017;49:300‐306.
  21. Wray H, Elliott J, Bailey SR, Harris PA, Menzies‐Gow NJ. Plasma concentrations of inflammatory markers in previously laminitic ponies.. Equine Vet J 2013;45:546‐551.
  22. Carter RA, Treiber KH, Geor RJ. Prediction of incipient pasture‐associated laminitis from hyperinsulinaemia, hyperleptinaemia and generalised and localised obesity in a cohort of ponies.. Equine Vet J 2009;41:171‐178.
  23. Kemp KL, Skinner JE, Bertin FR. Effect of phenylbutazone on insulin secretion in horses with insulin dysregulation.. J Vet Intern Med 2024;38:1177‐1184.
  24. Bertin FR, Taylor SD, Bianco AW, Sojka‐Kritchevsky JE. The effect of fasting duration on baseline blood glucose concentration, blood insulin concentration, glucose/insulin ratio, oral sugar test, and insulin response test results in horses.. J Vet Intern Med 2016;30:1726‐1731.
  25. de Laat MA, Sillence MN. The repeatability of an oral glucose test in ponies.. Equine Vet J 2017;49:238‐243.
  26. Hackett ES, McCue PM. Evaluation of a veterinary glucometer for use in horses.. J Vet Intern Med 2010;24:617‐621.
  27. Carslake HB, Pinchbeck GL, McGowan CM. Evaluation of a chemiluminescent immunoassay for measurement of equine insulin.. J Vet Intern Med 2017;31:568‐574.
  28. Schmidt O, Deegen E, Fuhrmann H, Dühlmeier R, Sallmann HP. Effects of fat feeding and energy level on plasma metabolites and hormones in Shetland ponies.. J Vet Med A Physiol Pathol Clin Med 2001;48:39‐49.
  29. Warnken T, Brehm R, Feige K, Huber K. Insulin signaling in various equine tissues under basal conditions and acute stimulation by intravenously injected insulin.. Domest Anim Endocrinol 2017;61:17‐26.
  30. Naylor JM, Kronfeld DS, Acland H. Hyperlipemia in horses: effects of undernutrition and disease.. Am J Vet Res 1980;41:899‐905.
  31. Frank N, Sojka JE, Latour MA. Effect of withholding feed on concentration and composition of plasma very low density lipoprotein and serum nonesterified fatty acids in horses.. Am J Vet Res 2002;63:1018‐1021.
  32. Clark BL, Stewart AJ, Kemp KL, Bamford NJ, Bertin FR. Evaluation of field‐testing protocols to diagnose insulin dysregulation in ponies using a Bayesian approach.. Vet J 2023;298‐299:106019.
  33. Frank N, Sommardahl CS, Eiler H, Webb LL, Denhart JW, Boston RC. Effects of oral administration of levothyroxine sodium on concentrations of plasma lipids, concentration and composition of very‐low‐density lipoproteins, and glucose dynamics in healthy adult mares.. Am J Vet Res 2005;66:1032‐1038.
  34. Kraegen EW, Cooney GJ. Free fatty acids and skeletal muscle insulin resistance.. Curr Opin Lipidol 2008;19:235‐241.
  35. Delarue J, Magnan C. Free fatty acids and insulin resistance.. Curr Opin Clin Nutr Metab Care 2007;10:142‐148.
  36. Burns TA, Geor RJ, Mudge MC, McCutcheon LJ, Hinchcliff KW, Belknap JK. Proinflammatory cytokine and chemokine gene expression profiles in subcutaneous and visceral adipose tissue depots of insulin‐resistant and insulin‐sensitive light breed horses.. J Vet Intern Med 2010;24:932‐939.
  37. Suagee JK, Corl BA, Geor RJ. A potential role for pro‐inflammatory cytokines in the development of insulin resistance in horses.. Animals 2012;2:243‐260.
  38. Zak A, Siwinska N, Elzinga S. Effects of equine metabolic syndrome on inflammation and acute‐phase markers in horses.. Domest Anim Endocrinol 2020;72:106448.
  39. Fitzgerald DM, Anderson ST, Sillence MN, de Laat MA. The cresty neck score is an independent predictor of insulin dysregulation in ponies.. PLoS One 2019;14:e0220203.
  40. Bamford NJ, Potter SJ, Harris PA, Bailey SR. Breed differences in insulin sensitivity and insulinemic responses to oral glucose in horses and ponies of moderate body condition score.. Domest Anim Endocrinol 2014;47:101‐107.
  41. Staub C, Venturi E, Cirot M. Ultrasonographic measures of body fatness and their relationship with plasma levels and adipose tissue expression of four adipokines in Welsh pony mares.. Domest Anim Endocrinol 2019;69:75‐83.
  42. Frank N, Andrews FM, Elliott SB, Lew J, Boston RC. Effects of rice bran oil on plasma lipid concentrations, lipoprotein composition, and glucose dynamics in mares.. J Anim Sci 2005;83:2509‐2518.

Citations

This article has been cited 1 times.
  1. Zhang Y, Wang C, Khan MZ, Ju Z, Huang J. Current Understanding of Bovine Ketosis: From Molecular Basis to Farm-Level Management. Animals (Basel) 2025 Dec 18;15(24).
    doi: 10.3390/ani15243644pubmed: 41463929google scholar: lookup
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