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Journal of veterinary internal medicine2024; doi: 10.1111/jvim.17199

Value of measuring markers of lipid metabolism in horses during an oral glucose test.

Abstract: Characterizing the lipid response to an oral glucose test (OGT) might improve our understanding of Equine Metabolic Syndrome. Objective: To describe the effects of an OGT on lipid metabolism and determine the value of measuring triglyceride and nonesterified fatty acid (NEFA) concentrations in hyperinsulinemic (HI) and insulin-resistant (IR) horses. Methods: Twenty horses including 7 HI-IR horses, 4 HI-non-IR horses, and 9 non-HI-non-IR horses (control). Methods: Cross-sectional design. Horses underwent an OGT, with blood samples collected at 0, 60, 90, and 120 minutes. Insulin, glucose, triglyceride, and NEFA concentrations were measured and compared over time and between groups, with P < .05 considered significant. Results: In all horses, the OGT had a significant effect on triglyceride concentrations (median [interquartile range]: .35 [.30-.50] mmol/L at 0 minute vs .25 [.21-.37] mmol/L at 120 minutes, P = .005) and on NEFA concentrations (.1 [.1-.2] mEq/L at 0 minute vs .05 [.05-.1] mEq/L at 120 minutes, P = .0009). However, horses with HI and IR had higher triglyceride areas under the curve (AUC, 79.46 ± 46.59 vs 33.32 ± 6.75 mmol/L*min, P = .01) as well as NEFA AUC (9.1 ± 2.9 vs 6.0 ± 6.8 mEq/L*min, P = .03) than control horses. No significant difference was detected between control and HI non-IR horses. Conclusions: Determining triglyceride and NEFA concentrations might help assess tissue insulin resistance during an OGT.
© 2024 The Author(s). Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
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Publication Date: 2024-09-18 PubMed ID: 39291576DOI: 10.1111/jvim.17199Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research seeks to understand the effect of an oral glucose test (OGT) on the lipid metabolism of horses, particularly those with hyperinsulinemia (HI) and insulin resistance (IR), by measuring triglyceride and nonesterified fatty acid (NEFA) concentrations. The results indicate altered lipid metabolism in HI and IR horses which could assist in assessing tissue insulin resistance.

Research Objective

  • The main goal of this study was to unravel the impact of an oral glucose test (OGT) on lipid metabolism in horses. The study was designed specifically to establish the significance of measuring triglyceride and nonesterified fatty acid (NEFA) concentrations in hyperinsulinemic (HI) and insulin-resistant (IR) horses.

Research Methods

  • Twenty horses participated in the study, among them 7 were HI-IR, 4 were HI-non-IR, and 9 were non-HI-non-IR horses, acting as the control group.
  • The researchers utilized a cross-sectional design in their approach and carried out an Oral Glucose Test (OGT).
  • Blood samples were collected at four different intervals: 0, 60, 90, and 120 minutes during the test.
  • The researchers assessed Insulin, glucose, triglyceride, and NEFA concentrations and compared these results between the different time intervals and groups, considering a P-value of less than .05 as statistically significant.

Research Results

  • The OGT significantly affected both triglyceride and NEFA concentrations in all the horses. Triglyceride levels moderately decreased, and NEFA concentrations fell noticeably over time.
  • However, horses suffering from HI and IR displayed higher triglyceride and NEFA areas under the curve (AUC) when compared to the control group.
  • No notable difference was found between the control group and the HI-non-IR horses.

Research Conclusion

  • These findings suggest that measuring triglyceride and NEFA concentrations during an OGT can be valuable in evaluating tissue insulin resistance in horses.
  • This study adds to the growing body of research on Equine Metabolic Syndrome, particularly the role of glucose and lipid metabolism.

Cite This Article

APA
Zemek CHK, Kemp KL, Bertin FR. (2024). Value of measuring markers of lipid metabolism in horses during an oral glucose test. J Vet Intern Med. https://doi.org/10.1111/jvim.17199

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English

Researcher Affiliations

Zemek, Claire H K
  • School of Veterinary Science, The University of Queensland, Gatton, Queensland, Australia.
Kemp, Kate L
  • School of Veterinary Science, The University of Queensland, Gatton, Queensland, Australia.
Bertin, François-René
  • School of Veterinary Science, The University of Queensland, Gatton, Queensland, Australia.
  • College of Veterinary Medicine, Purdue University, West-Lafayette, Indiana, USA.

Grant Funding

  • D19-EQ-302 / Morris Animal Foundation
  • VETS5017 / The University of Queensland School of Veterinary Science

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Citations

This article has been cited 1 times.
  1. Zhang Y, Wang C, Khan MZ, Ju Z, Huang J. Current Understanding of Bovine Ketosis: From Molecular Basis to Farm-Level Management. Animals (Basel) 2025 Dec 18;15(24).
    doi: 10.3390/ani15243644pubmed: 41463929google scholar: lookup
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