Variation in the MC1R, ASIP, and MATP genes responsible for coat color in Kiso horse as determined by SNaPshot™ genotyping.
Abstract: Kiso horse is a breed of Japanese native horses. In this study, to clarify coat color gene variation in Kiso horses, we used SNaPshot™ genotyping to evaluate variation in MC1R, ASIP, and MATP genes at the Extension (E), Agouti (A), and Cream dilution (C) loci. The coat color of 149 horses was documented. The coat color of 140, 3, and 6 horses was bay, chestnut, and buckskin, respectively. Furthermore, the frequency of alleles E, e, A, a, C, and Cr was 0.80, 0.20, 0.86, 0.14, 0.98, and 0.02, respectively. Current status of coat color genes in Kiso horses was clarified, and this information will help plan further conservation of the horses.
Publication Date: 2018-11-22 PubMed ID: 30464090PubMed Central: PMC6361661DOI: 10.1292/jvms.18-0458Google Scholar: Lookup
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- Journal Article
Summary
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This research article reveals an investigation into the genetic factors responsible for coat color differences in the Kiso breed of Japanese horses using a gene-typing method called SNaPshot™. The target genes studied were MC1R, ASIP, and MATP, which are linked to coat color in horses, and the results aim to assist in future conservation efforts for this breed.
Objective of the Research
- The main objective of this study was to determine the variations in three specific genes (MC1R, ASIP, and MATP) that are responsible for the coat color in Kiso horses. The researchers used the SNaPshot™ genotyping method to achieve this.
Research Methodology
- Through the use of the SNaPshot™ genotyping, the researchers assessed the variations in the three mentioned genes at different locations (loci) namely the Extension (E), Agouti (A), and Cream dilution (C).
- The study sample included 149 Kiso horses whose coat colors were documented.
Outcomes
- Out of the total horses, 140 had a bay coat color, 3 were chestnuts, and 6 were buckskin.
- The frequency of alleles E, e, A, a, C, and Cr (which represent the genetic variations at the loci) was found to be 0.80, 0.20, 0.86, 0.14, 0.98, and 0.02, respectively. This data depicts the distribution of these genetic variants amongst the examined Kiso horses.
Implications
- The findings of this research bring clarity to the status of coat color genes in the Kiso horses, exposing the genetic basis behind different coat colors within the breed.
- Furthermore, the information derived from the study can aid in the planning of future conservation strategies for Kiso horses, by providing insights regarding the genetic diversity and distribution in the breed.
Cite This Article
APA
Nakamura K, Tozaki T, Kakoi H, Owada S, Takasu M.
(2018).
Variation in the MC1R, ASIP, and MATP genes responsible for coat color in Kiso horse as determined by SNaPshot™ genotyping.
J Vet Med Sci, 81(1), 100-102.
https://doi.org/10.1292/jvms.18-0458 Publication
Researcher Affiliations
- Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
- Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
- Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan.
- Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan.
- Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
- Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
- Education and Research Center for Food Animal Health (GeFAH), Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan.
MeSH Terms
- Agouti Signaling Protein / genetics
- Animals
- Genetic Variation
- Genotyping Techniques / veterinary
- Hair Color / genetics
- Horses / genetics
References
This article includes 13 references
- Bowling AT. Genetics of colour variation. pp. 53–70.
- Cieslak M, Reissmann M, Hofreiter M, Ludwig A. Colours of domestication.. Biol Rev Camb Philos Soc 2011 Nov;86(4):885-99.
- Ito M. Life with Kiso Horses. .
- Japan Equine Affairs Association. The Kiso horse. pp. 51–62.
- Kakoi H, Tozaki T, Gawahara H. Molecular analysis using mitochondrial DNA and microsatellites to infer the formation process of Japanese native horse populations.. Biochem Genet 2007 Apr;45(3-4):375-95.
- Kakoi H, Tozaki T, Nagata S, Gawahara H, Kijima-Suda I. Development of a method for simultaneously genotyping multiple horse coat colour loci and genetic investigation of basic colour variation in Thoroughbred and Misaki horses in Japan.. J Anim Breed Genet 2009 Dec;126(6):425-31.
- Mukoyama H, Furuta H, Yoshida T, Tomogane H, Yoshimura I. Application of a coat color gene polymorphism to evaluation of the bay monotonous phenomenon in Japanese native horse: Kiso pony. DNA Polymorph 15: 42–50.
- Rieder S, Taourit S, Mariat D, Langlois B, Guérin G. Mutations in the agouti (ASIP), the extension (MC1R), and the brown (TYRP1) loci and their association to coat color phenotypes in horses (Equus caballus).. Mamm Genome 2001 Jun;12(6):450-5.
- Takasu M, Hiramatsu N, Tozaki T, Kakoi H, Hasegawa T, Maeda M, Kusuda S, Doi O, Murase T, Mukoyama H. Population statistics and biological traits of endangered kiso horse.. J Equine Sci 2011;22(4):67-72.
- Takasu M, Hiramatsu N, Tozaki T, Kakoi H, Nakagawa T, Hasegawa T, Huricha, Maeda M, Murase T, Mukoyama H. Genetic characterization of the endangered Kiso horse using 31 microsatellite DNAs.. J Vet Med Sci 2012 Feb;74(2):161-6.
- Takasu M, Ishihara N, Tozaki T, Kakoi H, Maeda M, Mukoyama H. Genetic diversity of maternal lineage in the endangered Kiso horse based on polymorphism of the mitochondrial DNA D-loop region.. J Vet Med Sci 2014 Nov;76(11):1451-6.
- Tozaki T, Takezaki N, Hasegawa T, Ishida N, Kurosawa M, Tomita M, Saitou N, Mukoyama H. Microsatellite variation in Japanese and Asian horses and their phylogenetic relationship using a European horse outgroup.. J Hered 2003 Sep-Oct;94(5):374-80.
- Wagner HJ, Reissmann M. New polymorphism detected in the horse MC1R gene.. Anim Genet 2000 Aug;31(4):289-90.
Citations
This article has been cited 1 times.- Huang X, Otecko NO, Peng M, Weng Z, Li W, Chen J, Zhong M, Zhong F, Jin S, Geng Z, Luo W, He D, Ma C, Han J, Ommeh SC, Zhang Y, Zhang X, Du B. Genome-wide genetic structure and selection signatures for color in 10 traditional Chinese yellow-feathered chicken breeds.. BMC Genomics 2020 Apr 20;21(1):316.
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