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Home / Equine Research Bank / J Anim Sci / Viability of stored equine embryos.
Journal of animal science1987; 65(2); 534-542; doi: 10.2527/jas1987.652534x

Viability of stored equine embryos.

Abstract: Equine embryos were recovered nonsurgically 6.5 d after ovulation (Exp. 1) and those greater than 200 microns were stored in one of three media: 1) Ham's F10 + 10% fetal calf serum (FCS) under 5% CO2, 5% O2 and 90% N2 at 24 C (Ham's F10); 2) Minimal Essential Medium with Hank's balanced salts + 10% FCS in air (MEM) at 24 C or 3) MEM at 5 C n = 10/treatment). Embryos less than or equal to 200 micron (n = 10) were bisected microsurgically; one-half of each embryo was stored in Ham's F10 and the other half in either Dulbecco's phosphate-buffered saline + 10% FCS in air at 24 C (DPBS), or MEM in air at 24 C. At 0, 12 and 24 h, embryos were: 1) measured; 2) assigned a developmental score of 1 to 4 (1 = tight morula, 4 = expanding blastocyst) and 3) assigned a quality score of 1 to 5 (1 = excellent, 5 = degenerate). Whole embryos stored in MEM at 5 C or 24 C did not (P greater than .05) advance in development by 24 h, whereas those stored in Ham's F10 at 24 C were more (P less than .05) advanced (i.e., higher developmental score) by 24 h. From 0 to 24 h, 1 of 10, 6 of 10 and 7 of 10 whole embryos developed when stored in MEM 5 C, MEM 24 C and Ham's F10 24 C, respectively. Embryo quality was better at 24 h (P less than .05) for embryos stored in Ham's F10 at 24 C compared with MEM at 5 C.(ABSTRACT TRUNCATED AT 250 WORDS)
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Publication Date: 1987-08-01 PubMed ID: 3624099DOI: 10.2527/jas1987.652534xGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studies the survival rate of equine embryos stored in different media and temperature conditions. The research findings indicate that embryos stored in Ham’s F10 medium at 24 Celsius degree show better development and quality compared to those stored in a Minimal Essential Medium (MEM) at either 5 or 24 Celsius degree.

Objective and Methodology

  • The aim of the study was to understand the viability of equine embryos when stored in different conditions. This research’s results could have important implications for the future of equine reproduction and embryo storage.
  • The embryos were collected non-surgically 6.5 days after ovulation.
  • The equine embryos larger than 200 microns were stored in either Ham’s F10 medium at 24 C under certain gas conditions, Minimal Essential Medium (MEM) with Hank’s balanced salts at 24 C, or MEM at 5 C.
  • Embryos equal to or less than 200 micron were divided into two halves and stored in either Ham’s F10, Dulbecco’s phosphate-buffered saline at 24 C or MEM.

Measurements and Scoring

  • The embryos were measured and assigned a developmental score based on their stage of development (from 1=tight morula to 4=expanding blastocyst) and a quality score (from 1=excellent to 5=degenerate) at 0, 12 and 24 hour intervals.
  • Observations showed that whole embryos stored in MEM at 5 C or 24 C did not advance in development by 24 hours. This insight introduces the possibility that Ham’s F10 at 24 C could provide a more conducive environment for the development of embryos.

Results and Conclusion

  • The research found that embryos stored in Ham’s F10 solution at 24 C (as opposed to 5 C) showed significant developmental and qualitative improvements at the end of the 24 hour measurement period.
  • These findings suggest that the storage environment can significantly impact the viability and quality of equine embryos, which could inform future procedures in equine embryo preservation and ultimately lead to better outcomes in equine reproduction.

Cite This Article

APA
Clark KE, Squires EL, McKinnon AO, Seidel GE. (1987). Viability of stored equine embryos. J Anim Sci, 65(2), 534-542. https://doi.org/10.2527/jas1987.652534x

Publication

Journal of animal science
ISSN: 0021-8812
NlmUniqueID: 8003002
Country: United States
Language: English
Volume: 65
Issue: 2
Pages: 534-542

Researcher Affiliations

Clark, K E
    Squires, E L
      McKinnon, A O
        Seidel, G E

          MeSH Terms

          • Animals
          • Culture Media
          • Horses / embryology
          • Tissue Preservation / veterinary

          Citations

          This article has been cited 7 times.
          1. D' Fonseca NMM, Gibson CME, van Doorn DA, Roelfsema E, de Ruijter-Villani M, Stout TAE. Effect of Overfeeding Shetland Pony Mares on Embryonic Glucose and Lipid Accumulation, and Expression of Imprinted Genes. Animals (Basel) 2021 Aug 26;11(9).
            doi: 10.3390/ani11092504pubmed: 34573470google scholar: lookup
          2. D'Fonseca NMM, Gibson CME, Hummel I, van Doorn DA, Roelfsema E, Stout TAE, van den Broek J, de Ruijter-Villani M. Overfeeding Extends the Period of Annual Cyclicity but Increases the Risk of Early Embryonic Death in Shetland Pony Mares. Animals (Basel) 2021 Feb 1;11(2).
            doi: 10.3390/ani11020361pubmed: 33535548google scholar: lookup
          3. do Nascimento AD, Marques JCC, Cezar ARR, Batista AM, Kastelic JP, Câmara DR. Inhibition of Na(+), K(+) -ATPase with ouabain is detrimental to equine blastocysts. Anim Reprod 2020 Jan 22;17(1).
            doi: 10.21451/1984-3143-AR2019-0079pubmed: 32368275google scholar: lookup
          4. Martinez CA, Cambra JM, Nohalez A, Parrilla I, Roca J, Vazquez JL, Rodriguez-Martinez H, Gil MA, Martinez EA, Cuello C. Prevention of hatching of porcine morulae and blastocysts by liquid storage at 20 °C. Sci Rep 2019 Apr 17;9(1):6219.
            doi: 10.1038/s41598-019-42712-xpubmed: 30996298google scholar: lookup
          5. Martinez EA, Angel MA, Cuello C, Sanchez-Osorio J, Gomis J, Parrilla I, Vila J, Colina I, Diaz M, Reixach J, Vazquez JL, Vazquez JM, Roca J, Gil MA. Successful non-surgical deep uterine transfer of porcine morulae after 24 hour culture in a chemically defined medium. PLoS One 2014;9(8):e104696.
            doi: 10.1371/journal.pone.0104696pubmed: 25118944google scholar: lookup
          6. Grau N, Aparicio B, Escrich L, Mercader A, Delgado A, Remohí J, Escribá MJ. Short-term storage of tripronucleated human embryos. J Assist Reprod Genet 2013 Aug;30(8):1043-7.
            doi: 10.1007/s10815-013-0036-8pubmed: 23820799google scholar: lookup
          7. Poitras P, Guay P, Vaillancourt D, Zidane N, Bigras-Poulin M. In vitro viability of cryopreserved equine embryos following different freezing protocols. Can J Vet Res 1994 Oct;58(4):235-41.
            pubmed: 7889453
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