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Home / Equine Research Bank / BMC Cell Biol / Virally and physically transgenized equine adipose-derived s...
BMC cell biology2010; 11; 73; doi: 10.1186/1471-2121-11-73

Virally and physically transgenized equine adipose-derived stromal cells as a cargo for paracrine secreted factors.

Abstract: Adipose-Derived Stromal Cells have been shown to have multiple lineage differentiation properties and to be suitable for tissues regeneration in many degenerative processes. Their use has been proposed for the therapy of joint diseases and tendon injuries in the horse. In the present report the genetic manipulation of Equine Adipose-Derived Stromal Cells has been investigated. Results: Equine Adipose-Derived Stromal Cells were successfully virally transduced as well as transiently and stably transfected with appropriate parameters, without detrimental effect on their differentiation properties. Moreover, green fluorescent protein alone, fused to neo gene, or co-expressed as bi-cistronic reporter constructs, driven by viral and house-keeping gene promoters, were tested. The better expressed cassette was employed to stably transfect Adipose-Derived Stromal Cells for cell therapy purposes. Stably transfected Equine Adipose-Derived Stromal Cells with a heterologous secreted viral antigen were able to immunize horses upon injection into the lateral wall of the neck. Conclusions: This study provides the methods to successfully transgenize Adipose-Derived Stromal Cells both by lentiviral vector and by transfection using optimized constructs with suitable promoters and reporter genes. In conclusion these findings provide a working platform for the delivery of potentially therapeutic proteins to the site of cells injection via transgenized Equine Adipose-Derived Stromal Cells.
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Publication Date: 2010-09-23 PubMed ID: 20863390PubMed Central: PMC2949624DOI: 10.1186/1471-2121-11-73Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the genetic manipulation of horse Adipose-Derived Stromal Cells (ASCs), focusing on their virally and physically transgenic attributes. It proves successful transgenization of ASCs and provides a platform for potentially therapeutic protein delivery to the sites of cell injection.

Background

  • The study revolves around Equine Adipose-Derived Stromal Cells (ASCs), which are known for their multiple lineage differentiation properties and suitability for tissue regeneration in degenerative processes.
  • Given their regenerative potential, the use of these cells has been suggested in the treatment of joint diseases and tendon injuries in horses.

Research Findings

  • Researchers explored the genetic manipulation of ASCs. They were successfully virally transduced as well as transiently and stably transfected using appropriate parameters. The process did not negatively impact the differentiation properties of the cells.
  • Tests were conducted on green fluorescent protein alone, fused to neo gene, or co-expressed as bi-cistronic reporter constructs. These were driven by viral and house-keeping gene promoters.
  • The best expressed cassette was then used to stably transfect ASCs for cell therapy.
  • Stably transfected Equine ASCs with a heterologous secreted viral antigen proved to be capable of immunizing horses upon injection into the lateral wall of the neck, thus presenting them as a potential cargo for paracrine secreted factors.

Conclusions and Implications

  • The methods provided in the research for successful transgenization of ASCs both by lentiviral vector and by transfection have utility in future research and potential therapeutic applications.
  • This research helps in laying a groundwork for the delivery of potentially therapeutic proteins to the site of cell injection via transgenized ASCs. The process used in the study can be used as a successful platform for cell therapy.

Cite This Article

APA
Donofrio G, Capocefalo A, Franceschi V, Morini G, Del Bue M, Conti V, Cavirani S, Grolli S. (2010). Virally and physically transgenized equine adipose-derived stromal cells as a cargo for paracrine secreted factors. BMC Cell Biol, 11, 73. https://doi.org/10.1186/1471-2121-11-73

Publication

BMC cell biology
ISSN: 1471-2121
NlmUniqueID: 100966972
Country: England
Language: English
Volume: 11
Pages: 73

Researcher Affiliations

Donofrio, Gaetano
  • Dipartimento di Salute Animale, Sezione di Malattie Infettive, Facoltà di Medicina Veterinaria, via del Taglio 10, 43100 Parma, Italy. gaetano.donofrio@unipr.it
Capocefalo, Antonio
    Franceschi, Valentina
      Morini, Giorgio
        Del Bue, Maurizio
          Conti, Virna
            Cavirani, Sandro
              Grolli, Stefano

                MeSH Terms

                • Adipose Tissue / cytology
                • Animals
                • Antigens, Viral / genetics
                • Antigens, Viral / immunology
                • Antigens, Viral / metabolism
                • Cell Differentiation / genetics
                • Cloning, Molecular
                • Feasibility Studies
                • Genetic Therapy
                • Guided Tissue Regeneration
                • Horses
                • Immunity / genetics
                • Immunization
                • Joint Diseases / pathology
                • Joint Diseases / therapy
                • Lentivirus Infections / genetics
                • Lentivirus Infections / immunology
                • Lentivirus Infections / therapy
                • Lentiviruses, Equine
                • Stem Cell Transplantation
                • Stromal Cells / immunology
                • Stromal Cells / metabolism
                • Stromal Cells / pathology
                • Stromal Cells / transplantation
                • Transduction, Genetic / methods
                • Transfection / methods

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                Citations

                This article has been cited 6 times.
                1. Bastos RG, Franceschi V, Tebaldi G, Connelley T, Morrison WI, Knowles DP, Donofrio G, Fry LM. Molecular and Antigenic Properties of Mammalian Cell-Expressed Theileria parva Antigen Tp9. Front Immunol 2019;10:897.
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