Western blot assay using recombinant p26 antigen for detection of equine infectious anemia virus-specific antibodies.
Abstract: We analyzed the performance of a single-band Western blot (WB) test using recombinant p26 (rp26) capsid protein of equine infectious anemia virus. According to the results obtained, the rp26 WB test is a reliable confirmatory diagnostic tool to be used as a complementary test after an enzyme-linked immunosorbent assay or agar gel immunodiffusion test yielding doubtful results.
Publication Date: 2007-10-24 PubMed ID: 17959820PubMed Central: PMC2168377DOI: 10.1128/CVI.00293-07Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
- Antibodies
- Antigen
- Clinical Study
- Diagnosis
- Diagnostic Technique
- Disease
- Disease Diagnosis
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Equine Diseases
- Equine Health
- Equine Infectious Anemia
- Horses
- Immunology
- Infection
- Infectious Disease
- Laboratory Methods
- Protein
- Veterinary Medicine
- Veterinary Research
- Virus
- Western Blot
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article investigates the effectiveness of a single-band Western blot assay, utilizing recombinant p26 capsid proteins to detect antibodies of the equine infectious anemia virus. The study found that this particular Western blot test could serve as a reliable diagnostic tool for confirming results from other tests which may yield ambiguous outcomes.
Background of the Research
- The research focuses on improving the efficiency of diagnosing equine infectious anemia virus (EIAV), a retroviral disease that affects horses.
- Previous methods for diagnosing the virus include the enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion test (AGID). Both tests can, at times, render questionable results necessitating additional confirmatory tests.
Method Detail and Reasoning
- The researchers utilized a method called a single-band Western blot (WB) test for their study.
- This specific WB test differed from others as it used recombinant p26 (rp26) capsid protein. The p26 protein is a primary and integral part of the EIAV viral capsule, and hence, is likely to prompt an immune response in the host, i.e., horses.
- By incorporating the rp26 protein in the diagnostic tool, the researchers hypothesized that it might enhance the accuracy and reliability of detecting the EIAV-specific antibodies in the test sample.
Outcomes of the Research
- The outcomes showed that the rp26 WB test was indeed reliable in detecting EIAV-specific antibodies. More so, in comparison to previously used tests like ELISA and AGID, which often gave doubtful results.
- The success of the rp26 WB test shows promise as a supplemental confirmatory test for diagnosing EIAV after initial screening processes.
Significance of the Research
- The research provides an effective methodology using rp26 WB test for confirming EIAV diagnosis after ambiguous results – this additionally aids in avoiding false positives or negatives.
- The findings could potentially expedite the diagnostic process for EIAV, aiding veterinarians and horse caregivers in early disease detection and treatment.
- Faster and more accurate diagnoses of this virus will contribute to the overall health of horse populations and prevent potential outbreaks of EIAV.
Cite This Article
APA
Alvarez I, Gutierrez G, Ostlund E, Barrandeguy M, Trono K.
(2007).
Western blot assay using recombinant p26 antigen for detection of equine infectious anemia virus-specific antibodies.
Clin Vaccine Immunol, 14(12), 1646-1648.
https://doi.org/10.1128/CVI.00293-07 Publication
Researcher Affiliations
- Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, INTA, C.C. 1712, Castelar, Argentina. ialvarez@cnia.inta.gov.ar
MeSH Terms
- Animals
- Antibodies, Viral / analysis
- Blotting, Western / methods
- Blotting, Western / standards
- Blotting, Western / veterinary
- Enzyme-Linked Immunosorbent Assay / veterinary
- Escherichia coli / genetics
- Horses
- Immunodiffusion / methods
- Immunodiffusion / standards
- Immunodiffusion / veterinary
- Infectious Anemia Virus, Equine / genetics
- Infectious Anemia Virus, Equine / immunology
- Infectious Anemia Virus, Equine / isolation & purification
- Recombinant Proteins / chemistry
- Recombinant Proteins / immunology
- Reproducibility of Results
- Serologic Tests / veterinary
- Viral Core Proteins / chemistry
- Viral Core Proteins / genetics
- Viral Core Proteins / immunology
References
This article includes 13 references
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- Langemeier JL, Cook SJ, Cook RF, Rushlow KE, Montelaro RC, Issel CJ. Detection of equine infectious anemia viral RNA in plasma samples from recently infected and long-term inapparent carrier animals by PCR.. J Clin Microbiol 1996 Jun;34(6):1481-7.
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- Secretaria de Agricultura, Ganadería, Pesca y Alimentos. Resolución 617/05. Programa de Control y Erradicación de Enfermedades Equinas. 2005.
- Sentsui H, Inoshima Y, Murakami K, Akashi H, Purevtseren B, Pagmajav O, Sugiura T. Cross reaction of recombinant equine infectious anemia virus antigen to heterologous strains and application for serological survey among horses in the field.. Microbiol Immunol 2001;45(1):45-50.
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Citations
This article has been cited 4 times.- Hu Z, Guo K, Du C, Sun J, Naletoski I, Chu X, Lin Y, Wang X, Barrandeguy M, Samuel M, Wang W, Lau PI, Wernery U, Raghavan R, Wang X. Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia.. Appl Microbiol Biotechnol 2023 May;107(10):3305-3317.
- Yang G, Zhou B, Chen K, Hu Z, Guo W, Wang X, Du C. Diagnostic Performance of Competitive ELISA and Western Blot Methods for the Detection of Antibodies against Theileria equi and Babesia caballi.. Microorganisms 2022 Dec 21;11(1).
- Singha H, Goyal SK, Malik P, Khurana SK, Singh RK. Development, evaluation, and laboratory validation of immunoassays for the diagnosis of equine infectious anemia (EIA) using recombinant protein produced from a synthetic p26 gene of EIA virus.. Indian J Virol 2013 Dec;24(3):349-56.
- Awinda PO, Mealey RH, Williams LB, Conrad PA, Packham AE, Reif KE, Grause JF, Pelzel-McCluskey AM, Chung C, Bastos RG, Kappmeyer LS, Howe DK, Ness SL, Knowles DP, Ueti MW. Serum antibodies from a subset of horses positive for Babesia caballi by competitive enzyme-linked immunosorbent assay demonstrate a protein recognition pattern that is not consistent with infection.. Clin Vaccine Immunol 2013 Nov;20(11):1752-7.
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