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Home / Equine Research Bank / Sci Rep / Whole genome analysis reveals aneuploidies in early pregnanc...
Scientific reports2020; 10(1); 13314; doi: 10.1038/s41598-020-69967-z

Whole genome analysis reveals aneuploidies in early pregnancy loss in the horse.

Abstract: The first 8 weeks of pregnancy is a critical time, with the majority of pregnancy losses occurring during this period. Abnormal chromosome number (aneuploidy) is a common finding in human miscarriage, yet is rarely reported in domestic animals. Equine early pregnancy loss (EPL) has no diagnosis in over 80% of cases. The aim of this study was to characterise aneuploidies associated with equine EPL. Genomic DNA from clinical cases of spontaneous miscarriage (EPLs; 14-65 days of gestation) and healthy control placentae (various gestational ages) were assessed using a high density genotyping array. Aneuploidy was detected in 12/55 EPLs (21.8%), and 0/15 healthy control placentae. Whole genome sequencing (30X) and digital droplet PCR (ddPCR) validated results. The majority of these aneuploidies have never been reported in live born equines, supporting their embryonic/fetal lethality. Aneuploidies were detected in both placental and fetal compartments. Rodents are currently used to study how maternal ageing impacts aneuploidy risk, however the differences in reproductive biology is a limitation of this model. We present the first evidence of aneuploidy in naturally occurring equine EPLs at a similar rate to human miscarriage. We therefore suggest the horse as an alternative to rodent models to study mechanisms resulting in aneuploid pregnancies.
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Publication Date: 2020-08-07 PubMed ID: 32769994PubMed Central: PMC7415156DOI: 10.1038/s41598-020-69967-zGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study focuses on analyzing aneuploidy – the condition of having an abnormal number of chromosomes, and its potential role in early pregnancy loss in horses. The study finds a significant correlation between aneuploidy and early pregnancy loss (up to 8 weeks), proposing the horse as a valuable alternative to rodents as a research model for investigating the influences in aneuploid pregnancies.

Objective of the Study

  • The primary aim of this study is to identify and understand the role of aneuploidies (abnormal number of chromosomes) in equine early pregnancy loss (EPL). To achieve this, the researchers compared the genomes of samples from horses who had suffered early miscarriages with those of healthy pregnancies.

Methodology

  • The researchers used a high-density genotyping array to assess genomic DNA derived from clinical cases of spontaneous miscarriage (between 14-65 days of gestation) and from healthy control placentae of various gestational ages.
  • Furthermore, techniques such as whole-genome sequencing (30X) and digital droplet PCR (ddPCR) were employed to validate the results obtained from the genotyping array.

Results and Findings

  • The study found that aneuploidy was present in 12 of the 55 miscarriage cases (21.8%), while none were detected in the 15 healthy control placentae.
  • Most of the detected aneuploidies had never been reported in live-born equines, suggesting their possible role in causing embryonic/fetal lethality.
  • The researchers found such anomalies in both the placental and fetal compartments, indicating the widespread nature of these abnormalities.

Conclusion and Further Research

  • Based on these findings, this study presents the first evidence of aneuploidy in naturally occurring equine EPLs at a similar rate to human miscarriage.
  • Given the significant similarities in reproductive biology between humans and horses, the study suggests the utility of the horse as an alternative research model to rodents for studying the mechanisms leading to aneuploid pregnancies.

Cite This Article

APA
Shilton CA, Kahler A, Davis BW, Crabtree JR, Crowhurst J, McGladdery AJ, Wathes DC, Raudsepp T, de Mestre AM. (2020). Whole genome analysis reveals aneuploidies in early pregnancy loss in the horse. Sci Rep, 10(1), 13314. https://doi.org/10.1038/s41598-020-69967-z

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 10
Issue: 1
Pages: 13314
PII: 13314

Researcher Affiliations

Shilton, Charlotte A
  • Department of Comparative Biomedical Sciences, The Royal Veterinary College, University of London, London, UK.
Kahler, Anne
  • Department of Comparative Biomedical Sciences, The Royal Veterinary College, University of London, London, UK.
Davis, Brian W
  • Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX, USA.
Crabtree, James R
  • Equine Reproductive Services (UK) Ltd., North Yorkshire, UK.
Crowhurst, James
  • Newmarket Equine Hospital, Newmarket, Suffolk, UK.
McGladdery, Andrew J
  • Rossdales Equine Practice, Newmarket, UK.
Wathes, D Claire
  • Department of Production and Population Health, The Royal Veterinary College, University of London, Hatfield, UK.
Raudsepp, Terje
  • Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX, USA.
de Mestre, Amanda M
  • Department of Comparative Biomedical Sciences, The Royal Veterinary College, University of London, London, UK. ademestre@rvc.ac.uk.

MeSH Terms

  • Abortion, Veterinary / genetics
  • Abortion, Veterinary / pathology
  • Aneuploidy
  • Animals
  • Female
  • Genome
  • Genome-Wide Association Study
  • Horse Diseases / genetics
  • Horse Diseases / pathology
  • Horses
  • Pregnancy
  • Whole Genome Sequencing

Grant Funding

  • TBA/EPL/2018 / The Thoroughbred Breeders Association

Conflict of Interest Statement

The authors declare no competing interests.

References

This article includes 64 references
  1. Macklon NS, Geraedts JP, Fauser BC. Conception to ongoing pregnancy: the 'black box' of early pregnancy loss.. Hum Reprod Update 2002 Jul-Aug;8(4):333-43.
    pubmed: 12206468doi: 10.1093/humupd/8.4.333google scholar: lookup
  2. Rose BV, Firth M, Morris B, Roach JM, Wathes DC, Verheyen KLP, de Mestre AM. Descriptive study of current therapeutic practices, clinical reproductive findings and incidence of pregnancy loss in intensively managed thoroughbred mares.. Anim Reprod Sci 2018 Jan;188:74-84.
    pubmed: 29146097doi: 10.1016/j.anireprosci.2017.11.011google scholar: lookup
  3. Wiltbank MC, Baez GM, Garcia-Guerra A, Toledo MZ, Monteiro PL, Melo LF, Ochoa JC, Santos JE, Sartori R. Pivotal periods for pregnancy loss during the first trimester of gestation in lactating dairy cows.. Theriogenology 2016 Jul 1;86(1):239-53.
    pubmed: 27238438doi: 10.1016/j.theriogenology.2016.04.037google scholar: lookup
  4. Morris LH, Allen WR. Reproductive efficiency of intensively managed Thoroughbred mares in Newmarket.. Equine Vet J 2002 Jan;34(1):51-60.
    pubmed: 11822372doi: 10.2746/042516402776181222google scholar: lookup
  5. de Mestre AM, Rose BV, Chang YM, Wathes DC, Verheyen KLP. Multivariable analysis to determine risk factors associated with early pregnancy loss in thoroughbred broodmares.. Theriogenology 2019 Jan 15;124:18-23.
    pubmed: 30326374doi: 10.1016/j.theriogenology.2018.10.008google scholar: lookup
  6. Ricketts S, Barrelet A, Whitwell K. Equine abortion. Equine Vet. Educ. 2003;15:18–21.
  7. Lear TL, Layton G. Use of zoo-FISH to characterise a reciprocal translocation in a thoroughbred mare: t(1;1 6)(q16;q21.3).. Equine Vet J 2002 Mar;34(2):207-9.
    pubmed: 11902765doi: 10.2746/042516402776767295google scholar: lookup
  8. Lear TL, Lundquist J, Zent WW, Fishback WD Jr, Clark A. Three autosomal chromosome translocations associated with repeated early embryonic loss (REEL) in the domestic horse (Equus caballus).. Cytogenet Genome Res 2008;120(1-2):117-22.
    pubmed: 18467834doi: 10.1159/000118749google scholar: lookup
  9. Durkin K, Raudsepp T, Chowdhary BP. Cytogenetic evaluation of the stallion. Equine Reprod. 1462–1468 (2011).
  10. Lear TL, Raudsepp T, Lundquist JM, Brown SE. Repeated early embryonic loss in a thoroughbred mare with a chromosomal translocation [64, XX, t (2; 13)]. J. Equine Vet. Sci. 2014;34:805–809.
  11. Ghosh S, Das PJ, Avila F, Thwaits BK, Chowdhary BP, Raudsepp T. A Non-Reciprocal Autosomal Translocation 64,XX, t(4;10)(q21;p15) in an Arabian Mare with Repeated Early Embryonic Loss.. Reprod Domest Anim 2016 Feb;51(1):171-4.
    pubmed: 26547799doi: 10.1111/rda.12636google scholar: lookup
  12. Power MM. The first description of a balanced reciprocal translocation [t(1q;3q)] and its clinical effects in a mare.. Equine Vet J 1991 Mar;23(2):146-9.
    pubmed: 2044510doi: 10.1111/j.2042-3306.1991.tb02742.xgoogle scholar: lookup
  13. Nybo Andersen AM, Wohlfahrt J, Christens P, Olsen J, Melbye M. Maternal age and fetal loss: population based register linkage study.. BMJ 2000 Jun 24;320(7251):1708-12.
    pmc: PMC27416pubmed: 10864550doi: 10.1136/bmj.320.7251.1708google scholar: lookup
  14. Ball BA, Hillman RB, Woods GL. Survival of equine embryos transferred to normal and subfertile mares.. Theriogenology 1987 Aug;28(2):167-74.
    pubmed: 16726302doi: 10.1016/0093-691x(87)90264-0google scholar: lookup
  15. Brinsko SP, Ball BA, Ellington JE. In vitro maturation of equine oocytes obtained from different age groups of sexually mature mares.. Theriogenology 1995 Sep;44(4):461-9.
    pubmed: 16727745doi: 10.1016/0093-691x(95)00218-wgoogle scholar: lookup
  16. Rizzo M, Ducheyne KD, Deelen C, Beitsma M, Cristarella S, Quartuccio M, Stout TAE, de Ruijter-Villani M. Advanced mare age impairs the ability of in vitro-matured oocytes to correctly align chromosomes on the metaphase plate.. Equine Vet J 2019 Mar;51(2):252-257.
    pmc: PMC6585749pubmed: 30025174doi: 10.1111/evj.12995google scholar: lookup
  17. Chen S, Liu D, Zhang J, Li S, Zhang L, Fan J, Luo Y, Qian Y, Huang H, Liu C, Zhu H, Jiang Z, Xu C. A copy number variation genotyping method for aneuploidy detection in spontaneous abortion specimens.. Prenat Diagn 2017 Feb;37(2):176-183.
    pubmed: 27977861doi: 10.1002/pd.4986google scholar: lookup
  18. Hutaff-Lee C, Cordeiro L, Tartaglia N. Cognitive and medical features of chromosomal aneuploidy.. Handb Clin Neurol 2013;111:273-9.
    pubmed: 23622175doi: 10.1016/b978-0-444-52891-9.00030-0google scholar: lookup
  19. Pai GS, Lewandowski RC, Borgaonkar DS. Handbook of Chromosomal Syndromes. Hoboken: Wiley; 2003.
  20. Herzog A, Hoehn H. [Two additional cases of autosomal trisomy, 61,XY,+12 and 61,XX,+12, in cattle].. Cytogenet Cell Genet 1991;57(4):211-3.
    pubmed: 1743077doi: 10.1159/000133149google scholar: lookup
  21. Buoen LC, Zhang TQ, Weber AF, Turner T, Bellamy J, Ruth GR. Arthrogryposis in the foal and its possible relation to autosomal trisomy.. Equine Vet J 1997 Jan;29(1):60-2.
    pubmed: 9031866doi: 10.1111/j.2042-3306.1997.tb01638.xgoogle scholar: lookup
  22. Brito LF. Autosomic 27 trisomy in a standardbred colt. J. Equine Vet. Sci. 2008;28:431–436.
  23. Holl HM, Lear TL, Nolen-Walston RD, Slack J, Brooks SA. Detection of two equine trisomies using SNP-CGH.. Mamm Genome 2013 Jun;24(5-6):252-6.
    pubmed: 23515943doi: 10.1007/s00335-013-9450-6google scholar: lookup
  24. Bowling AT, Millon LV. Two autosomal trisomies in the horse: 64,XX,-26,+t(26q26q) and 65,XX,+30.. Genome 1990 Oct;33(5):679-82.
    pubmed: 2262139doi: 10.1139/g90-101google scholar: lookup
  25. Lear TL, Cox JH, Kennedy GA. Autosomal trisomy in a Thoroughbred colt: 65,XY,+31.. Equine Vet J 1999 Jan;31(1):85-8.
    pubmed: 9952335doi: 10.1111/j.2042-3306.1999.tb03796.xgoogle scholar: lookup
  26. Klunder LR, McFeely RA, Beech J, McClune W. Autosomal trisomy in a Standardbred colt.. Equine Vet J 1989 Jan;21(1):69-70.
    pubmed: 2920704doi: 10.1111/j.2042-3306.1989.tb02092.xgoogle scholar: lookup
  27. Power MM. Equine half sibs with an unbalanced X;15 translocation or trisomy 28.. Cytogenet Cell Genet 1987;45(3-4):163-8.
    pubmed: 3691181doi: 10.1159/000132448google scholar: lookup
  28. Raudsepp T, Chowdhary BP. Chromosome Aberrations and Fertility Disorders in Domestic Animals.. Annu Rev Anim Biosci 2016;4:15-43.
    pubmed: 26884101doi: 10.1146/annurev-animal-021815-111239google scholar: lookup
  29. Schmutz SM, Moker JS, Clark EG, Orr JP. Chromosomal aneuploidy associated with spontaneous abortions and neonatal losses in cattle.. J Vet Diagn Invest 1996 Jan;8(1):91-5.
    pubmed: 9026087doi: 10.1177/104063879600800114google scholar: lookup
  30. Rambags BP, Krijtenburg PJ, Drie HF, Lazzari G, Galli C, Pearson PL, Colenbrander B, Stout TA. Numerical chromosomal abnormalities in equine embryos produced in vivo and in vitro.. Mol Reprod Dev 2005 Sep;72(1):77-87.
    pubmed: 15948165doi: 10.1002/mrd.20302google scholar: lookup
  31. Viuff D, Rickords L, Offenberg H, Hyttel P, Avery B, Greve T, Olsaker I, Williams JL, Callesen H, Thomsen PD. A high proportion of bovine blastocysts produced in vitro are mixoploid.. Biol Reprod 1999 Jun;60(6):1273-8.
    pubmed: 10330080doi: 10.1095/biolreprod60.6.1273google scholar: lookup
  32. Murray JD, Moran C, Boland MP, Nancarrow CD, Sutton R, Hoskinson RM, Scaramuzzi RJ. Polyploid cells in blastocysts and early fetuses from Australian Merino sheep.. J Reprod Fertil 1986 Nov;78(2):439-46.
    pubmed: 3806508doi: 10.1530/jrf.0.0780439google scholar: lookup
  33. Blue MG. A cytogenetical study of prenatal loss in the mare.. Theriogenology 1981 Mar;15(3):295-309.
    pubmed: 16725589doi: 10.1016/0093-691x(81)90051-0google scholar: lookup
  34. Haynes SE, Reisner AH. Cytogenetic and DNA analyses of equine abortion.. Cytogenet Cell Genet 1982;34(3):204-14.
    pubmed: 7140374doi: 10.1159/000131808google scholar: lookup
  35. Rose BV, Cabrera-Sharp V, Firth MJ, Barrelet FE, Bate S, Cameron IJ, Crabtree JR, Crowhurst J, McGladdery AJ, Neal H, Pynn J, Pynn OD, Smith C, Wise Z, Verheyen KL, Wathes DC, de Mestre AM. A method for isolating and culturing placental cells from failed early equine pregnancies.. Placenta 2016 Feb;38:107-11.
    pubmed: 26907389doi: 10.1016/j.placenta.2015.12.014google scholar: lookup
  36. Schaefer RJ, Schubert M, Bailey E, Bannasch DL, Barrey E, Bar-Gal GK, Brem G, Brooks SA, Distl O, Fries R, Finno CJ, Gerber V, Haase B, Jagannathan V, Kalbfleisch T, Leeb T, Lindgren G, Lopes MS, Mach N, da Câmara Machado A, MacLeod JN, McCoy A, Metzger J, Penedo C, Polani S, Rieder S, Tammen I, Tetens J, Thaller G, Verini-Supplizi A, Wade CM, Wallner B, Orlando L, Mickelson JR, McCue ME. Developing a 670k genotyping array to tag ~2M SNPs across 24 horse breeds.. BMC Genomics 2017 Jul 27;18(1):565.
    pmc: PMC5530493pubmed: 28750625doi: 10.1186/s12864-017-3943-8google scholar: lookup
  37. Wapner RJ, Simpson JL, Golbus MS, Zachary JM, Ledbetter DH, Desnick RJ, Fowler SE, Jackson LG, Lubs H, Mahony RJ. Chorionic mosaicism: association with fetal loss but not with adverse perinatal outcome.. Prenat Diagn 1992 May;12(5):347-55.
    pubmed: 1523202doi: 10.1002/pd.1970120504google scholar: lookup
  38. Kalousek DK, Barrett IJ, Gärtner AB. Spontaneous abortion and confined chromosomal mosaicism.. Hum Genet 1992 Mar;88(6):642-6.
    pubmed: 1551667doi: 10.1007/bf02265289google scholar: lookup
  39. Jia CW, Wang L, Lan YL, Song R, Zhou LY, Yu L, Yang Y, Liang Y, Li Y, Ma YM, Wang SY. Aneuploidy in Early Miscarriage and its Related Factors.. Chin Med J (Engl) 2015 Oct 20;128(20):2772-6.
    pmc: PMC4736891pubmed: 26481744doi: 10.4103/0366-6999.167352google scholar: lookup
  40. Nikitina TV, Sazhenova EA, Tolmacheva EN, Sukhanova NN, Kashevarova AA, Skryabin NA, Vasilyev SA, Nemtseva TN, Yuriev SY, Lebedev IN. Comparative Cytogenetic Analysis of Spontaneous Abortions in Recurrent and Sporadic Pregnancy Losses.. Biomed Hub 2016 Jan-Apr;1(1):1-11.
    pmc: PMC6945958pubmed: 31988885doi: 10.1159/000446099google scholar: lookup
  41. Wang F, Qi J, Yu T, Wang L, Zhang Z, Chen S, Zhao P, Yuan L. Novel karyotypes of partial monosomy 21 and partial monosomy 1 and underlying etiology.. Int J Clin Exp Pathol 2017;10(9):9765-9773.
    pmc: PMC6965908pubmed: 31966860
  42. Papavassiliou P, York TP, Gursoy N, Hill G, Nicely LV, Sundaram U, McClain A, Aggen SH, Eaves L, Riley B, Jackson-Cook C. The phenotype of persons having mosaicism for trisomy 21/Down syndrome reflects the percentage of trisomic cells present in different tissues.. Am J Med Genet A 2009 Feb 15;149A(4):573-83.
    pmc: PMC3707311pubmed: 19291777doi: 10.1002/ajmg.a.32729google scholar: lookup
  43. Roper RJ, Reeves RH. Understanding the basis for Down syndrome phenotypes.. PLoS Genet 2006 Mar;2(3):e50.
    pmc: PMC1420680pubmed: 16596169doi: 10.1371/journal.pgen.0020050google scholar: lookup
  44. Lebedev I. Mosaic aneuploidy in early fetal losses.. Cytogenet Genome Res 2011;133(2-4):169-83.
    pubmed: 21311181doi: 10.1159/000324120google scholar: lookup
  45. Hassold T, Hall H, Hunt P. The origin of human aneuploidy: where we have been, where we are going.. Hum Mol Genet 2007 Oct 15;16 Spec No. 2:R203-8.
    pubmed: 17911163doi: 10.1093/hmg/ddm243google scholar: lookup
  46. Bugno M, Słota E, Kościelny M. Karyotype evaluation among young horse populations in Poland.. Schweiz Arch Tierheilkd 2007 May;149(5):227-32.
    pubmed: 17557614doi: 10.1024/0036-7281.149.5.227google scholar: lookup
  47. Cimadomo D, Fabozzi G, Vaiarelli A, Ubaldi N, Ubaldi FM, Rienzi L. Impact of Maternal Age on Oocyte and Embryo Competence.. Front Endocrinol (Lausanne) 2018;9:327.
    pmc: PMC6033961pubmed: 30008696doi: 10.3389/fendo.2018.00327google scholar: lookup
  48. Ge ZJ, Schatten H, Zhang CL, Sun QY. Oocyte ageing and epigenetics.. Reproduction 2015 Mar;149(3):R103-14.
    pmc: PMC4397590pubmed: 25391845doi: 10.1530/rep-14-0242google scholar: lookup
  49. Keefe DL, Niven-Fairchild T, Powell S, Buradagunta S. Mitochondrial deoxyribonucleic acid deletions in oocytes and reproductive aging in women.. Fertil Steril 1995 Sep;64(3):577-83.
    pubmed: 7641914
  50. Battaglia DE, Goodwin P, Klein NA, Soules MR. Influence of maternal age on meiotic spindle assembly in oocytes from naturally cycling women.. Hum Reprod 1996 Oct;11(10):2217-22.
    pubmed: 8943533doi: 10.1093/oxfordjournals.humrep.a019080google scholar: lookup
  51. Chiang T, Duncan FE, Schindler K, Schultz RM, Lampson MA. Evidence that weakened centromere cohesion is a leading cause of age-related aneuploidy in oocytes.. Curr Biol 2010 Sep 14;20(17):1522-8.
    pmc: PMC2939204pubmed: 20817534doi: 10.1016/j.cub.2010.06.069google scholar: lookup
  52. Ricketts SW, Alonso S. The effect of age and parity on the development of equine chronic endometrial disease.. Equine Vet J 1991 May;23(3):189-92.
    pubmed: 1884699doi: 10.1111/j.2042-3306.1991.tb02752.xgoogle scholar: lookup
  53. Franasiak JM, Forman EJ, Hong KH, Werner MD, Upham KM, Treff NR, Scott RT Jr. The nature of aneuploidy with increasing age of the female partner: a review of 15,169 consecutive trophectoderm biopsies evaluated with comprehensive chromosomal screening.. Fertil Steril 2014 Mar;101(3):656-663.e1.
    pubmed: 24355045doi: 10.1016/j.fertnstert.2013.11.004google scholar: lookup
  54. Bugno-Poniewierska M, Kozub D, Pawlina K, Tischner M Jr, Tischner M, Słota E, Wnuk M. Determination of the correlation between stallion's age and number of sex chromosome aberrations in spermatozoa.. Reprod Domest Anim 2011 Oct;46(5):787-92.
    pubmed: 21323752doi: 10.1111/j.1439-0531.2010.01742.xgoogle scholar: lookup
  55. Kushnir V, Scott R, Frattarelli J. Effect of paternal age on aneuploidy rates in first trimester pregnancy loss. J. Med. Genet. Genomics. 2010;2:38–43.
  56. Carp H, Toder V, Aviram A, Daniely M, Mashiach S, Barkai G. Karyotype of the abortus in recurrent miscarriage.. Fertil Steril 2001 Apr;75(4):678-82.
    pubmed: 11287018doi: 10.1016/s0015-0282(00)01801-xgoogle scholar: lookup
  57. Fujino Y, Ozaki K, Yamamasu S, Ito F, Matsuoka I, Hayashi E, Nakamura H, Ogita S, Sato E, Inoue M. DNA fragmentation of oocytes in aged mice.. Hum Reprod 1996 Jul;11(7):1480-3.
    pubmed: 8671488doi: 10.1093/oxfordjournals.humrep.a019421google scholar: lookup
  58. Hamatani T, Falco G, Carter MG, Akutsu H, Stagg CA, Sharov AA, Dudekula DB, VanBuren V, Ko MS. Age-associated alteration of gene expression patterns in mouse oocytes.. Hum Mol Genet 2004 Oct 1;13(19):2263-78.
    pubmed: 15317747doi: 10.1093/hmg/ddh241google scholar: lookup
  59. Carnevale EM. The mare model for follicular maturation and reproductive aging in the woman.. Theriogenology 2008 Jan 1;69(1):23-30.
    pubmed: 17976712doi: 10.1016/j.theriogenology.2007.09.011google scholar: lookup
  60. Cabrera-Sharp V, Read JE, Richardson S, Kowalski AA, Antczak DF, Cartwright JE, Mukherjee A, de Mestre AM. SMAD1/5 signaling in the early equine placenta regulates trophoblast differentiation and chorionic gonadotropin secretion.. Endocrinology 2014 Aug;155(8):3054-64.
    pmc: PMC4183921pubmed: 24848867doi: 10.1210/en.2013-2116google scholar: lookup
  61. Robbin MG, Wagner B, Noronha LE, Antczak DF, de Mestre AM. Subpopulations of equine blood lymphocytes expressing regulatory T cell markers.. Vet Immunol Immunopathol 2011 Mar 15;140(1-2):90-101.
    pubmed: 21208665doi: 10.1016/j.vetimm.2010.11.020google scholar: lookup
  62. Raudsepp T, Durkin K, Lear TL, Das PJ, Avila F, Kachroo P, Chowdhary BP. Molecular heterogeneity of XY sex reversal in horses.. Anim Genet 2010 Dec;41 Suppl 2:41-52.
    pubmed: 21070275doi: 10.1111/j.1365-2052.2010.02101.xgoogle scholar: lookup
  63. Chiang C, Layer RM, Faust GG, Lindberg MR, Rose DB, Garrison EP, Marth GT, Quinlan AR, Hall IM. SpeedSeq: ultra-fast personal genome analysis and interpretation.. Nat Methods 2015 Oct;12(10):966-8.
    pmc: PMC4589466pubmed: 26258291doi: 10.1038/nmeth.3505google scholar: lookup
  64. Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R. The Sequence Alignment/Map format and SAMtools.. Bioinformatics 2009 Aug 15;25(16):2078-9.
    pmc: PMC2723002pubmed: 19505943doi: 10.1093/bioinformatics/btp352google scholar: lookup

Citations

This article has been cited 15 times.
  1. Roach J, Arango Sabogal JC, Smith K, Foote A, Verheyen K, de Mestre AM. Multivariable analysis to determine risk factors associated with abortion in mares. Reprod Fertil 2022 Nov 1;3(4):301-12.
    doi: 10.1530/RAF-22-0087pubmed: 36374277google scholar: lookup
  2. Pauciullo A, Versace C, Perucatti A, Gaspa G, Li LY, Yang CY, Zheng HY, Liu Q, Shang JH. Oocyte aneuploidy rates in river and swamp buffalo types (Bubalus bubalis) determined by Multi-color Fluorescence In Situ Hybridization (M-FISH). Sci Rep 2022 May 19;12(1):8440.
    doi: 10.1038/s41598-022-12603-9pubmed: 35590020google scholar: lookup
  3. Ghosh S, Kjöllerström J, Metcalfe L, Reed S, Juras R, Raudsepp T. The Second Case of Non-Mosaic Trisomy of Chromosome 26 with Homologous Fusion 26q;26q in the Horse. Animals (Basel) 2022 Mar 22;12(7).
    doi: 10.3390/ani12070803pubmed: 35405793google scholar: lookup
  4. Avidor-Reiss T, Achinger L, Uzbekov R. The Centriole's Role in Miscarriages. Front Cell Dev Biol 2022;10:864692.
    doi: 10.3389/fcell.2022.864692pubmed: 35300410google scholar: lookup
  5. Antczak DF, Allen WRT. Placentation in Equids. Adv Anat Embryol Cell Biol 2021;234:91-128.
    doi: 10.1007/978-3-030-77360-1_6pubmed: 34694479google scholar: lookup
  6. Schneider I, de Ruijter-Villani M, Hossain MJ, Stout TAE, Ellenberg J. Dual spindles assemble in bovine zygotes despite the presence of paternal centrosomes. J Cell Biol 2021 Nov 1;220(11).
    doi: 10.1083/jcb.202010106pubmed: 34550316google scholar: lookup
  7. Benammar A, Derisoud E, Vialard F, Palmer E, Ayoubi JM, Poulain M, Chavatte-Palmer P. The Mare: A Pertinent Model for Human Assisted Reproductive Technologies?. Animals (Basel) 2021 Aug 4;11(8).
    doi: 10.3390/ani11082304pubmed: 34438761google scholar: lookup
  8. Bugno-Poniewierska M, Raudsepp T. Horse Clinical Cytogenetics: Recurrent Themes and Novel Findings. Animals (Basel) 2021 Mar 16;11(3).
    doi: 10.3390/ani11030831pubmed: 33809432google scholar: lookup
  9. Ryan CA, Berry DP, Bugno-Poniewierska M, Burke MK, Raudsepp T, Egan S, Doyle JL. Two Cases of Chromosome 27 Trisomy in Horses Detected Using Illumina BeadChip Genotyping. Animals (Basel) 2025 Jun 22;15(13).
    doi: 10.3390/ani15131842pubmed: 40646741google scholar: lookup
  10. Fakhar-I-Adil M, Angel-Velez D, Araftpoor E, Amin QA, Hedia M, Bühler M, Gevaert K, Menten B, Van Soom A, Chuva de Sousa Lopes SM, Stoop D, De Roo C, Smits K, Heindryckx B. Biphasic CAPA-IVM Improves Equine Oocyte Quality and Subsequent Embryo Development Without Inducing Genetic Aberrations. Int J Mol Sci 2025 Jun 8;26(12).
    doi: 10.3390/ijms26125495pubmed: 40564960google scholar: lookup
  11. Switonski M, Szczerbal I, Nowacka-Woszuk J. From cytogenetics to cytogenomics: a new era in the diagnosis of chromosomal abnormalities in domestic animals. J Appl Genet 2025 Sep;66(3):661-673.
    doi: 10.1007/s13353-025-00943-xpubmed: 39869248google scholar: lookup
  12. Delaval A, Glover KA, Solberg MF, Fjelldal PG, Hansen T, Harvey AC. Chromosomal aberrations and early mortality in a non-mammalian vertebrate: example from pressure-induced triploid Atlantic salmon. Heredity (Edinb) 2024 Dec;133(6):426-436.
    doi: 10.1038/s41437-024-00727-9pubmed: 39369146google scholar: lookup
  13. Valera M, Karlau A, Anaya G, Bugno-Poniewierska M, Molina A, Encina A, Azor PJ, Demyda-Peyrás S. The Use of Genomic Screening for the Detection of Chromosomal Abnormalities in the Domestic Horse: Five New Cases of 65,XXY Syndrome in the Pura Raza Español Breed. Animals (Basel) 2024 Sep 3;14(17).
    doi: 10.3390/ani14172560pubmed: 39272345google scholar: lookup
  14. Lawson JM, Salem SE, Miller D, Kahler A, van den Boer WJ, Shilton CA, Sever T, Mouncey RR, Ward J, Hampshire DJ, Foote AK, Bryan JS, Juras R, Pynn OD, Davis BW, Bellone RR, Raudsepp T, de Mestre AM. Naturally occurring horse model of miscarriage reveals temporal relationship between chromosomal aberration type and point of lethality. Proc Natl Acad Sci U S A 2024 Aug 13;121(33):e2405636121.
    doi: 10.1073/pnas.2405636121pubmed: 39102548google scholar: lookup
  15. De Coster T, Zhao Y, Tšuiko O, Demyda-Peyrás S, Van Soom A, Vermeesch JR, Smits K. Genome-wide equine preimplantation genetic testing enabled by simultaneous haplotyping and copy number detection. Sci Rep 2024 Jan 23;14(1):2003.
    doi: 10.1038/s41598-023-48103-7pubmed: 38263320google scholar: lookup
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