Wound-healing markers after autologous and allogeneic epithelial-like stem cell treatment.
Abstract: Several cytokines and growth factors play an essential role in skin regeneration and epithelial-like stem cells (EpSCs) have beneficial effects on wound healing in horses. However, there are no reports available on the expression of these growth factors and cytokines after EpSC therapy. Methods: Wounds of 6 cm(2) were induced in the gluteus region of 6 horses and treated with (i) autologous EpSCs, (ii) allogeneic EpSCs, (iii) vehicle treatment or (iv) untreated control. Real time polymerase chain reaction was performed on tissue biopsies taken 1 and 5 weeks after these treatments to evaluate mRNA expression of interferon (IFN)-γ, interleukin (IL)-6, vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), insulin-like growth factor (IGF)-1 and epidermal keratin (eKER). Results: One week after treatments, mRNA levels of IL-6 (P = 0.012) and VEGF (P = 0.008) were higher in allogeneic EpSC-treated wounds compared with controls. Also, mRNA levels of IGF-1 were higher at 1 week in both autologous (P = 0.027) and allogeneic (P = 0.035) EpSC-treated wounds. At week 5, all EpSC- and vehicle-treated wounds demonstrated significantly higher IFN-γ, VEGF and eKER mRNA expression compared with controls and compared with their respective levels at week 1. Conclusions: Equine wounds treated with allogeneic EpSCs demonstrate a significant increase in mRNA expression of IL-6, VEGF and IGF-1 in the acute phase. In the longer term, an increase in IFN-γ, VEGF and eKER mRNA was detected in the wounds treated with allogenic EpSCs, autologous EpSCs or their vehicle.
Copyright © 2016 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.
Publication Date: 2016-03-15 PubMed ID: 26971684DOI: 10.1016/j.jcyt.2016.01.008Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study examines how specific types of growth factors and cytokines, which help regenerate skin, respond once epithelial-like stem cell (EpSCs) therapy is applied in equine wounds. The results indicate that applying EpSCs, whether from the same individual (autologous) or from a different one (allogeneic), leads to a significant increase in mRNA expression of certain growth factors and cytokines, which are crucial in wound healing.
Research Methodology
- 6cm2 wounds were induced in the gluteus region of six horses which were then treated with autologous EpSCs, allogeneic EpSCs, vehicle treatment, or left untreated as a form of control.
- A real time polymerase chain reaction was conducted on tissue biopsies obtained 1 week and 5 weeks after these treatments.
- This was done to monitor mRNA expression of several markers like interferon (IFN)-γ, interleukin (IL)-6, vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), insulin-like growth factor (IGF)-1, and epidermal keratin (eKER).
Research Findings
- After one week of the treatment, allogeneic EpSC-treated wounds showed higher mRNA levels of IL-6 and VEGF compared to controls. Moreover, both autologous and allogeneic EpSC treatments resulted in higher mRNA levels of IGF-1.
- At the 5-week observation point, all EpSC- and vehicle-treated wounds showed significantly higher IFN-γ, VEGF, and eKER mRNA expression compared to untreated wounds and compared to their respective levels at week 1.
Conclusions from the study
- Wounds treated with allogeneic EpSCs showed a significant increase in mRNA expression of IL-6, VEGF, and IGF-1 in the acute phase.
- In the long term, there was an increase in IFN-γ, VEGF, and eKER mRNA levels in wounds treated with either type of EpSCs or with the vehicle treatment. This suggests that these treatments stimulate the expression of key factors that play a significant role in wound healing.
The findings of the study highlight the potential of EpSC treatments in promoting wound healing, potentially providing a foundation for further research in stem cell therapy.
Cite This Article
APA
Spaas JH, Gomiero C, Broeckx SY, Van Hecke L, Maccatrozzo L, Martens A, Martinello T, Patruno M.
(2016).
Wound-healing markers after autologous and allogeneic epithelial-like stem cell treatment.
Cytotherapy, 18(4), 562-569.
https://doi.org/10.1016/j.jcyt.2016.01.008 Publication
Researcher Affiliations
- Global Stem cell Technology, ANACURA group, Evergem, Belgium; Pell Cell Medicals, ANACURA group, Evergem, Belgium. Electronic address: Jan.spaas@anacura.com.
- Department of Comparative Biomedicine and Food Science, University of Padova, Italy.
- Global Stem cell Technology, ANACURA group, Evergem, Belgium; Pell Cell Medicals, ANACURA group, Evergem, Belgium.
- Department of Surgery and Anaesthesia of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
- Department of Comparative Biomedicine and Food Science, University of Padova, Italy.
- Department of Surgery and Anaesthesia of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
- Department of Comparative Biomedicine and Food Science, University of Padova, Italy.
- Department of Comparative Biomedicine and Food Science, University of Padova, Italy. Electronic address: marco.pat@unipd.it.
MeSH Terms
- Animals
- Biomarkers / analysis
- Biomarkers / metabolism
- Cytokines / genetics
- Cytokines / metabolism
- Epidermis / metabolism
- Epithelial Cells / metabolism
- Epithelial Cells / transplantation
- Horses
- Insulin-Like Growth Factor I / genetics
- Insulin-Like Growth Factor I / metabolism
- Interleukin-6 / genetics
- Interleukin-6 / metabolism
- RNA, Messenger / analysis
- RNA, Messenger / metabolism
- Regeneration / genetics
- Skin / metabolism
- Stem Cell Transplantation / methods
- Stem Cells / metabolism
- Transplantation, Autologous
- Wound Healing / genetics
Citations
This article has been cited 3 times.- Mund SJK, Kawamura E, Awang-Junaidi AH, Campbell J, Wobeser B, MacPhee DJ, Honaramooz A, Barber S. Homing and Engraftment of Intravenously Administered Equine Cord Blood-Derived Multipotent Mesenchymal Stromal Cells to Surgically Created Cutaneous Wound in Horses: A Pilot Project. Cells 2020 May 8;9(5).
- He B, Chen J, Liu L, Wang H, Wang S, Li P, Zhou J. Knockdown of Tcf3 enhances the wound healing effect of bone marrow mesenchymal stem cells in rats. Biosci Rep 2019 Aug 30;39(8).
- Textor JA, Clark KC, Walker NJ, Aristizobal FA, Kol A, LeJeune SS, Bledsoe A, Davidyan A, Gray SN, Bohannon-Worsley LK, Woolard KD, Borjesson DL. Allogeneic Stem Cells Alter Gene Expression and Improve Healing of Distal Limb Wounds in Horses. Stem Cells Transl Med 2018 Jan;7(1):98-108.
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