Xenogenic bone matrix extracts induce osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells.
Abstract: Colloss and Colloss-E are sterile acellular lyophilizates extracted from bovine and equine bone matrix, respectively. Animal and clinical studies have shown that these xenogenic bone matrix extracts (BMEs) are effective as bone graft substitutes. In this report, we investigated the effect of Colloss and Colloss-E on human adult in vitro-expanded bone marrow-derived mesenchymal stem cells (BMMSCs). Specifically, we assessed whether these xenogenic BMEs induced osteoblastic differentiation of cultured BMMSC. We show that BMMSCs treated with either Colloss or Colloss-E exhibited characteristic osteoblastic morphological changes accompanied by the expression of osteoblast-specific markers, such as alkaline phosphatase activity, osteopontin secretion and calcium deposits, explicitly demonstrating that these bone matrix extracts induce osteoblastic differentiation of BMMSCs in vitro. Hence, xenogenic BMEs induce bone-specific differentiation of BMMSCs, presumably through providing stem cells with structural and soluble mediators that mimic the in vivo microenvironment. These results may explain the in vivo mode of action of these medical devices, and potentially provide a novel tissue engineering-based treatment of bone defect, using autologous BMMSCs pretreated with BMEs.
Publication Date: 2007-07-20 PubMed ID: 17635046DOI: 10.2217/17460751.2.4.383Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The researchers are investigating the effects of extracts from bovine and equine bone matrix on human bone marrow-derived stem cells. They found that these extracts can induce these stem cells to differentiate into bone cells, suggesting a new potential method for treating bone defects.
Objectives and Methods
- The objective of this study was to examine the impact of Colloss and Colloss-E, xenogenic bone matrix extracts (BMEs) sourced from bovine and equine bone matrix respectively, on human bone marrow-derived mesenchymal stem cells (BMMSCs). The researchers aimed to see if these BMEs could induce osteoblastic (bone cell) differentiation in the BMMSCs.
- The experimental methods included treating BMMSCs in vitro (under controlled laboratory conditions) with either Colloss or Colloss-E and observing the resultant effects. By monitoring specific markers associated with osteoblasts, such as alkaline phosphatase activity, osteopontin secretion and calcium deposits, the researchers could determine if the stem cells had transitioned into bone cells.
Findings
- The study discovered that BMMSCs treated with either Colloss or Colloss-E showed morphological changes typical of osteoblasts, as well as the expression of osteoblast-specific markers. This indicates that the bone matrix extracts successfully induced differentiation into bone cells in vitro.
- Notably, these findings imply that the BMEs may act by providing the stem cells with structural and soluble mediators that replicate the in vivo (within the organism) microenvironment.
Implications and Potential Applications
- The conclusions of this research may elucidate the in vivo action mechanism of these medical devices, i.e. how they function when used in living organisms. They suggest BMEs may prompt the differentiation of stem cells into bone cells.
- Importantly, the study could lead to a potential new method for treating bone defects. For instance, BMMSCs could be pretreated with BMEs, and then applied to bone defects to encourage bone regeneration.
Cite This Article
APA
El-Sabban ME, El-Khoury H, Hamdan-Khalil R, Sindet-Pedersen S, Bazarbachi A.
(2007).
Xenogenic bone matrix extracts induce osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells.
Regen Med, 2(4), 383-390.
https://doi.org/10.2217/17460751.2.4.383 Publication
Researcher Affiliations
- Department of Human Morphology, Faculty of Medicine, American University of Beirut, PO Box 113-0236, Beirut, Lebanon. me00@aub.edu.lb
MeSH Terms
- Adult
- Alkaline Phosphatase / metabolism
- Bone Marrow Cells / cytology
- Bone Marrow Cells / metabolism
- Calcium / metabolism
- Cell Differentiation
- Collagen / metabolism
- Humans
- Mesenchymal Stem Cells / cytology
- Mesenchymal Stem Cells / metabolism
- Osteoblasts / cytology
- Osteopontin / metabolism
Citations
This article has been cited 8 times.- Mukhtar AH, Alqutub MN. Comparison between Allogenic and Xenogenic Bone Blocks on the Osteogenic Potential of Cultured Human Periodontal Ligament Stem Cells: Confocal Laser and Scanning Electron Microscopy Study.. Int J Appl Basic Med Res 2021 Apr-Jun;11(2):75-79.
- Zhou H, Boys AJ, Harrod JB, Bonassar LJ, Estroff LA. Mineral Distribution Spatially Patterns Bone Marrow Stromal Cell Behavior on Monolithic Bone Scaffolds.. Acta Biomater 2020 Aug;112:274-285.
- Nassif L, El Sabban M. Mesenchymal Stem Cells in Combination with Scaffolds for Bone Tissue Engineering.. Materials (Basel) 2011 Oct 11;4(10):1793-1804.
- Zibara K, Hamdan R, Dib L, Sindet-Pedersen S, Kharfan-Dabaja M, Bazarbachi A, El-Sabban M. Acellular bone marrow extracts significantly enhance engraftment levels of human hematopoietic stem cells in mouse xeno-transplantation models.. PLoS One 2012;7(7):e40140.
- Baas J, Jakobsen T, Elmengaard B, Bechtold JE, Soballe K. The effect of adding an equine bone matrix protein lyophilisate on fixation and osseointegration of HA-coated Ti implants.. J Biomed Mater Res A 2012 Jan;100(1):188-94.
- Sollazzo V, Palmieri A, Girardi A, Zollino I, Brunelli G, Spinelli G, Carinci F. Osteoplant acts on stem cells derived from peripheral blood.. J Indian Soc Periodontol 2010 Jan;14(1):12-7.
- Jensen J, Foldager CB, Jakobsen TV, Søballe K, Bünger C, Baas J. Use of carboxymethyl cellulose and collagen carrier with equine bone lyophilisate suggests late onset bone regenerative effect in a humerus drill defect - a pilot study in six sheep.. Open Orthop J 2010 May 11;4:181-7.
- Ngangan AV, McDevitt TC. Acellularization of embryoid bodies via physical disruption methods.. Biomaterials 2009 Feb;30(6):1143-9.
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