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Home / Equine Research Bank / PLoS One / Y-chromosome analysis in Retuertas horses.
PloS one2013; 8(5); e64985; doi: 10.1371/journal.pone.0064985

Y-chromosome analysis in Retuertas horses.

Abstract: Several studies based on a variety of genetic markers have attempted to establish the origins of horse domestication. Thus far a discrepancy between the results of mitochondrial DNA analysis, which show high levels of diversity, and results from the Y-chromosome, with almost no genetic variability, has been identified. Most previous work on the horse Y-chromosome has focused on widespread, popular breeds or local Asian breeds. It is possible that these breeds represent a reduced set of the genetic variation present in the species. Additional genetic variation may be present in local breeds and ancient feral populations, such as the Retuertas horse in Spain. In this study we analyzed the Y-chromosome of the Retuertas horse, a feral horse population on the Iberian Peninsula that is at least several hundred years old, and whose genetic diversity and morphology suggests that it has been reproductively isolated for a long time. Data from the Retuertas horse was compared to another 11 breeds from the region (Portugal, Spain and France) or likely of Iberian origin, and then to data from 15 more breeds from around the globe. We sequenced 31 introns, Zinc finger Y-chromosomal protein (ZFY) and anonymous Y-linked fragments and genotyped 6 microsatellite loci found on the Y-chromosome. We found no sequence variation among all individuals and all breeds studied. However, fifteen differences were discovered between our data set and reference sequences in GenBank. We show that these likely represent errors within the deposited sequences, and suggest that they should not be used as comparative data for future projects.
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Publication Date: 2013-05-31 PubMed ID: 23741439PubMed Central: PMC3669082DOI: 10.1371/journal.pone.0064985Google Scholar: Lookup
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  • Journal Article
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research examines the Y-chromosome of Retuertas horses, which are native to the Iberian Peninsula and have been minimally influenced by crossbreeding. The study reveals that despite extensive analysis, no genetic variation was found across individuals and breeds, suggesting a potential error in previously known reference sequences.

Overview of the Study

  • The researchers conducted the study based on the premise that a majority of studies into horse domestication deduced from genetic markers showed a significant discrepancy, with mitochondrial DNA analysis indicating high levels of diversity and Y-chromosome analysis showing minimal genetic variability.
  • The focus was on Retuertas horses, a feral horse population in Spain, considering their ancient lineage and indications of reproductive isolation, thereby suggesting they might carry additional genetic variation.

Methodology and Comparative Analysis

  • The Y-chromosomes of Retuertas horses were examined and compared to eleven other breeds from Portugal, Spain, France, and additional breeds likely of Iberian heritage. They were also compared to fifteen different breeds globally.
  • The researchers sequenced several genetic elements, including 31 introns, Zinc finger Y-chromosomal protein (ZFY), and anonymous Y-linked fragments. They also genotyped 6 microsatellite loci present on the Y-chromosome.

Findings and Conclusion

  • No sequence variation was found across all individuals and all breeds studied, contradicting the expectation of discovering more genetic diversity in the Retuertas horse breed.
  • The researchers identified fifteen differences between the data set they produced and reference sequences available in GenBank, a genetic sequence database.
  • The discrepancies suggested potential errors in the reference sequences within the GenBank database, leading to the researchers’ recommendation that these sequences should not be used as comparative data in future research.

Cite This Article

APA
Brandariz-Fontes C, Leonard JA, Vega-Pla JL, Backström N, Lindgren G, Lippold S, Rico C. (2013). Y-chromosome analysis in Retuertas horses. PLoS One, 8(5), e64985. https://doi.org/10.1371/journal.pone.0064985

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 8
Issue: 5
Pages: e64985
PII: e64985

Researcher Affiliations

Brandariz-Fontes, Claudia
  • Estación Biológica de Doñana (EBD-CSIC), Seville, Spain.
Leonard, Jennifer A
    Vega-Pla, José Luis
      Backström, Niclas
        Lindgren, Gabriella
          Lippold, Sebastian
            Rico, Ciro

              MeSH Terms

              • Animals
              • Europe
              • Female
              • Genetic Loci
              • Genotype
              • Horses / genetics
              • Male
              • Microsatellite Repeats
              • Y Chromosome

              Conflict of Interest Statement

              The authors have declared that no competing interests exist.

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              Citations

              This article has been cited 11 times.
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