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Home / Equine Research Bank / Asian-Australas J Anim Sci / Y-Single Nucleotide Polymorphisms Diversity in Chinese Indig...
Asian-Australasian journal of animal sciences2015; 28(8); 1066-1074; doi: 10.5713/ajas.14.0784

Y-Single Nucleotide Polymorphisms Diversity in Chinese Indigenous Horse.

Abstract: In contrast to high genetic diversity of mitochondrial DNA (mtDNA), equine Y chromosome shows extremely low variability, implying limited patrilines in the domesticated horse. In this study, we applied direct sequencing and restriction fragment length polymorphism (RFLP) methods to investigate the polymorphisms of 33 Y chromosome specific loci in 304 Chinese indigenous horses from 13 breeds. Consequently, two Y-single nucleotide polymorphisms (SNPs) (Y-45701/997 and Y-50869) and one Y-indel (Y-45288) were identified. Of those, the Y-50869 (T>A) revealed the highest variation frequency (24.67%), whereas it was only 3.29% and 1.97% in Y-45288 (T/-) and Y-45701/997 (G>T) locus, respectively. These three mutations accounted for 27.96% of the total samples and identified five Y-SNP haplotypes, demonstrating genetic diversity of Y chromosome in Chinese horses. In addition, all the five Y-SNP haplotypes were shared by different breeds. Among 13 horse breeds analyzed, Balikun horse displayed the highest nucleotide diversity (π = 5.6×10(-4)) and haplotype diversity (h = 0.527), while Ningqiang horse showed the lowest nucleotide diversity (π = 0.00000) and haplotype diversity (h = 0.000). The results also revealed that Chinese horses had a different polymorphic pattern of Y chromosome from European and American horses. In conclusion, Chinese horses revealed genetic diversity of Y chromosome, however more efforts should be made to better understand the domestication and paternal origin of Chinese indigenous horses.
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Publication Date: 2015-06-25 PubMed ID: 26104513PubMed Central: PMC4478473DOI: 10.5713/ajas.14.0784Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates genetic diversity in the Y chromosomes of domestic horses from China, identifying three genetic mutations and contributing to our understanding of horse heritage.

Research Methodology

  • The researchers examined the Y chromosomes of 304 horses from 13 different Chinese breeds.
  • They used direct sequencing and restriction fragment length polymorphism (RFLP) methods to investigate polymorphisms, or variations, at 33 Y-chromosome-specific locations.

Research Findings

  • They found three specific genetic changes, known as Y-single nucleotide polymorphisms (SNPs). These consisted of two Y-SNPs (Y-45701/997 and Y-50869) and one insertion or deletion of genetic material, known as a Y-indel (Y-45288).
  • The Y-50869 (T>A) showed the highest variation frequency (24.67%), followed by Y-45288 (T/-) at 3.29% and Y-45701/997 (G>T) at 1.97%.
  • These mutations accounted for 27.96% of the total samples and identified five unique genetic blocks, or Y-SNP haplotypes. This revealed previously unknown diversity in the Y chromosomes of Chinese horses.
  • All five of these genetic blocks were found across different breeds.
  • The Balikun breed showed the highest level of genetic variation while Ningqiang horses had the lowest.

Conclusion

  • This study found evidence of significant genetic diversity in the Y chromosomes of horses from China. This is particularly noteworthy because the Y chromosome typically shows low levels of variation.
  • The findings were different from those seen in European and American horses, suggesting that Chinese horses have a unique evolutionary history and may represent separate lineages.
  • The researchers concluded that further work is needed to better understand the history and ancestry of indigenous Chinese horses.

Cite This Article

APA
Han H, Zhang Q, Gao K, Yue X, Zhang T, Dang R, Lan X, Chen H, Lei C. (2015). Y-Single Nucleotide Polymorphisms Diversity in Chinese Indigenous Horse. Asian-Australas J Anim Sci, 28(8), 1066-1074. https://doi.org/10.5713/ajas.14.0784

Publication

Asian-Australasian journal of animal sciences
ISSN: 1011-2367
NlmUniqueID: 9884245
Country: Korea (South)
Language: English
Volume: 28
Issue: 8
Pages: 1066-1074

Researcher Affiliations

Han, Haoyuan
  • College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China .
Zhang, Qin
  • College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China .
Gao, Kexin
  • College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China .
Yue, Xiangpeng
  • College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China .
Zhang, Tao
  • Shaanxi University of Technology, Hanzhong, Shaanxi 723001, China .
Dang, Ruihua
  • College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China .
Lan, Xianyong
  • College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China .
Chen, Hong
  • College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China .
Lei, Chuzhao
  • College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China .

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Citations

This article has been cited 8 times.
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