English
French
Current protocols.
Publisher:
John Wiley & Sons,
Frequency: Monthly
Country: United States
Language: English
Start Year:2021 -
Identifiers
| ISSN: | 2691-1299 (Electronic) 2691-1299 (Linking) |
| NLM ID: | 101773894 |
| (OCoLC): | 1147703173 |
| LCCN: | 2020200082 |
| Classification: | W1 |
Multiparametric Flow Cytometry for Determination of Viability, Caspase 3 and 7 Activity, and Lipid Peroxidation Adduct (4-Hydroxynonenal) in Equine Spermatozoa. Flow cytometry is a powerful tool for the analysis of cell samples formed of multipopulations, such as spermatozoa. In recent years, multiparametric cytometers have evolved, allowing the study of different cellular characteristics, such as protein expression, DNA analysis, or mitochondrial activity. Whether using traditional fluorescent dyes or fluorophore-conjugated antibodies, each cell or cellular component is individually stained, the sample is analyzed at high velocities, and then is displayed and interpreted in a dot-plot. We hereby describe the procedure to perform a multiparametric flo...
