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The EMBO journal.
Publisher:
Published for the European Molecular Biology Organization by IRL Press,. London : Wiley Blackwell (2014)
Frequency: Semimonthly, 1994-
Country: England
Language: English
Author(s):
European Molecular Biology Organization.
Start Year:1982 -
Identifiers
| ISSN: | 0261-4189 (Print) 1460-2075 (Electronic) 0261-4189 (Linking) |
| NLM ID: | 8208664 |
| (DNLM): | E05755000(s) |
| (OCoLC): | 08335096 |
| Coden: | EMJ0DG |
| LCCN: | 82643856 |
| Classification: | W1 EM369D |
Fusion pore expansion in horse eosinophils is modulated by Ca2+ and protein kinase C via distinct mechanisms. Using the patch-clamp technique, we studied the role of protein phosphorylation and dephosphorylation on the exocytotic fusion of secretory granules with the plasma membrane in horse eosinophils. Phorbol 12-myristate 13-acetate (PMA) had no effect on the amplitude and dynamics of degranulation, indicating that the formation of fusion pores is insensitive to activation of protein kinase C (PKC). Fusion pore expansion, however, was accelerated approximately 2-fold by PMA, and this effect was abolished by staurosporine. Elevating intracellular Ca2+ to 1.5 microM also resulted in a 2-fold accelera...
Focal exocytosis by eosinophils–compound exocytosis and cumulative fusion. We have investigated the granule fusion events during exocytosis in horse eosinophils by time-resolved patch-clamp capacitance measurements. Stimulation with intracellular GTP gamma S leads to a stepwise capacitance increase by 4.0 +/- 0.9 pF. At GTP gamma S concentrations < 20 microM the step size distribution is in agreement with the granule size distribution in resting cells. Above 80 microM the number of steps is reduced and very large steps occur. The total capacitance increase, however, is unaffected. These results show that at high GTP gamma S concentrations granule--granule fusion o...
