Placenta.
Periodical
Embryology
Obstetrics
Physiology
Placenta
Publisher:
W.B. Saunders.. Amsterdam : Elsevier (2010)
Frequency: Ten no. a year, 2001-
Country: Netherlands
Language: English
Start Year:1980 -
ISSN:
0143-4004 (Print)
1532-3102 (Electronic)
0143-4004 (Linking)
1532-3102 (Electronic)
0143-4004 (Linking)
Impact Factor
3.8
2022
| NLM ID: | 8006349 |
| (DNLM): | P16125000(s) |
| (OCoLC): | 05312765 |
| Coden: | PLACDF |
| LCCN: | 80647588 |
| Classification: | W1 PL101C |
The maternal leucocyte response to the endometrial cups in horses is correlated with the developmental stages of the invasive trophoblast cells.
Placenta
September 1, 1995
Volume 16, Issue 6 539-559 doi: 10.1016/s0143-4004(05)80005-0
Grünig G, Triplett L, Canady LK, Allen WR, Antczak DF.Invading trophoblasts form endometrial cups in the endometrium of the pregnant mare. In the present study we characterized the maternal leucocyte response to endometrial cups from their formation to their regression. The maternal leucocyte response was correlated with the stages of trophoblast development. (1) Aggregates of CD4+ and CD8+ cells were present between the migrating and differentiating endometrial cup trophoblasts and surrounding the forming endometrial cups. (2) Numbers of CD4+ cells within the mature endometrial cups were much reduced. At the periphery of the endometrial cups CD4... Release of lipid from the equine placenta during in vitro incubation.
Placenta
December 1, 1994
Volume 15, Issue 8 857-872 doi: 10.1016/s0143-4004(05)80187-0
Stammers JP, Hull D, Silver M, Fowden AL, Ousey J, Rossdale PD.An in vitro incubation technique was used to examine release of lipids from the equine placenta. Placental tissue was obtained at term (n = 5, term = 320-365 days) and earlier in gestation (n = 8, mean = 266 days). Term placentae were incubated at two temperatures, 4 degrees C (control) and 37 degrees C for 2 h. Pre-term placentae were incubated at 37 degrees C with two different concentrations of fatty acid in the medium. Tissues and media were analysed for their lipid concentrations. Term and pre-term placentae released free fatty acid (FFA) and phospholipid into the incubation medium during... Equine endometrium at pre-implantation stages of pregnancy has specific glycosylated regions.
Placenta
November 1, 1985
Volume 6, Issue 6 537-542 doi: 10.1016/s0143-4004(85)80007-2
Whyte A, Allen WR.Pre- and post-implantation endometrial tissue was removed from equids at known stages of pregnancy. Thin sections of this material were reacted with fluorescein conjugates of several lectins. Two lectins in particular, with specificities for L-fucose and N-acetylglucosamine, reacted strongly with the endometrial epithelium. The reaction for N-acetylglucosamine occurred in small patches on the endometrial epithelium and extended to cover the entire epithelial surface and endometrial glands after implantation. L-Fucose was observed in larger surface deposits particularly at the openings of endom... A review of implantation and early placentation in the mare.
Placenta
October 22, 2009
Volume 30, Issue 12 1005-1015 doi: 10.1016/j.placenta.2009.09.007
Allen WR, Wilsher S.Constant, self induced mobility throughout the uterine lumen between days 6 and 17 after ovulation, complete envelopment by a self-secreted glycoprotein capsule between days 7 and 30 and 'injection' of specialised, gonadotrophin-secreting trophoblast cells into the maternal endometrium at days 35-37 are three unusual aspects of equine embryogenesis. The outer trophoblast layer of the allantochorion finally establishes a stable, microvillous contact with the lumenal epithelium of the endometrium around days 40-42 and placentation commences thereafter. The allantochorion elongates steadily until... Read More
22
Inhibition of lymphocyte proliferation and activation: a mechanism used by equine invasive trophoblast to escape the maternal immune response.
Placenta
February 15, 2005
Volume 26, Issue 2-3 148-159 doi: 10.1016/j.placenta.2004.05.008
Flaminio MJ, Antczak DF.At days 36-38 of gestation, the equine invasive trophoblast cells migrate into the endometrium of the pregnant mare to form the endometrial cups. During their migration, they become surrounded by maternal CD4+ and CD8+ T lymphocytes, and stimulate a cytotoxic antibody response to the paternal major histocompatibility complex class I antigens that they express. Nevertheless, endometrial cup cells remain viable at the site of uterine invasion up to days 80-100 of gestation, suggesting the participation of immunomodulatory mechanisms to the maternal cellular immune response. To determine the effe... Read More
14
A structural and immunological study of chorionic gonadotrophin production by equine trophoblast girdle and cup cells.
Placenta
October 13, 2001
Volume 22, Issue 8-9 749-767 doi: 10.1053/plac.2001.0707
Wooding FB, Morgan G, Fowden AL, Allen WR.Equine chorionic gonadotropin (eCG) production by the fetally derived endometrial cups appears to be necessary for the establishment and maintenance of normal equine pregnancy. Starting at about the 27th day of pregnancy, an equatorial band of trophectodermal cells on the surface of the spherical conceptus forms the chorionic girdle. This girdle consists initially of flat trophectodermal epithelium which corrugates into folds as the cells proliferate. The folds are then pressed against the uterine epithelium by expansion of the conceptus. The cells on the apices of the folds become binucleate ... Read More
13
Molecular evidence for natural killer-like cells in equine endometrial cups.
Placenta
February 21, 2012
Volume 33, Issue 5 379-386 doi: 10.1016/j.placenta.2012.01.018
Noronha LE, Huggler KE, de Mestre AM, Miller DC, Antczak DF.To identify equine orthologs of major NK cell marker genes and utilize them to determine whether NK cells are present among the dense infiltration of lymphocytes that surround the endometrial cup structures of the horse placenta during early pregnancy. Methods: PCR primers were developed to detect the equine orthologs of NKP46, CD16, CD56, and CD94; gene expression was detected in RNA isolated from lymphocytes using standard 2-step reverse transcriptase (RT) PCR and products were cloned and sequenced. Absolute real-time RT-PCR was used to quantitate gene expression in total, CD3+, and CD3- per... Read More
11
Modeling trophoblast differentiation using equine chorionic girdle vesicles.
Placenta
December 3, 2007
Volume 29, Issue 2 158-169 doi: 10.1016/j.placenta.2007.10.005
de Mestre AM, Bacon SJ, Costa CC, Leadbeater JC, Noronha LE, Stewart F, Antczak DF.The chorionic girdle of the equine conceptus is comprised of specialized trophoblast cells which, at day 36-38 of equine pregnancy, gain an invasive phenotype and invade the endometrium to form endometrial cups. Studies of equine endometrial cups remain difficult to perform because of the invasive techniques required to obtain cup tissue and because sampling requires termination of the pregnancy. In this study we developed a system to model trophoblast differentiation and trophoblast-immune interactions in vitro and in vivo. We utilized a method of culturing chorionic girdle pieces in serum-fr... Read More
10
The maternal leucocyte response to the endometrial cups in horses is correlated with the developmental stages of the invasive trophoblast cells.
Placenta
September 1, 1995
Volume 16, Issue 6 539-559 doi: 10.1016/s0143-4004(05)80005-0
Grünig G, Triplett L, Canady LK, Allen WR, Antczak DF.Invading trophoblasts form endometrial cups in the endometrium of the pregnant mare. In the present study we characterized the maternal leucocyte response to endometrial cups from their formation to their regression. The maternal leucocyte response was correlated with the stages of trophoblast development. (1) Aggregates of CD4+ and CD8+ cells were present between the migrating and differentiating endometrial cup trophoblasts and surrounding the forming endometrial cups. (2) Numbers of CD4+ cells within the mature endometrial cups were much reduced. At the periphery of the endometrial cups CD4... The immunolocalization of Galectin-1 and Progesterone-Induced Blocking Factor (PIBF) in equine trophoblast: Possible roles in trophoblast invasion and the immunological protection of pregnancy.
Placenta
August 8, 2019
Volume 85 32-39 doi: 10.1016/j.placenta.2019.08.081
The proteins galectin-1 and Progesterone Induced Blocking Factor (PIBF) are present on human and murine trophoblast and are thought to influence both immunomodulation and trophoblast invasion. In equids, the invasive component of the placenta, the endometrial cups, stimulate maternal cell-mediated and humoral immune responses. It was therefore of interest to know if galectin-1 or PIBF could be immunolocalised to the invasive and/or non-invasive components of the equine placenta. Horse and mule (♀ horse X ♂ donkey) embryos and placental tissues between Days 12 and 124 of gestation were stai... Expression of glucose transporters in the endometrium and early conceptus membranes of the horse.
Placenta
June 25, 2018
Volume 68 23-32 doi: 10.1016/j.placenta.2018.06.308
Gibson C, de Ruijter-Villani M, Rietveld J, Stout TAE.Glucose is the primary energy substrate for early conceptus development and, for the first 40 days of gestation, the equine conceptus depends solely on glucose available in the histotroph; thereafter, histotrophic glucose provision continues to support transport across the definitive placenta. To investigate glucose provision routes during early equine pregnancy we examined expression of glucose transporters in conceptus membranes and endometrium recovered on days 7, 14, 21 and 28 after ovulation. To further differentiate the contributions of maternal progesterone priming and conceptus-endomet... Localisation of epidermal growth factor (EGF), its specific receptor (EGF-R) and aromatase at the materno-fetal interface during placentation in the pregnant mare.
Placenta
December 23, 2016
Volume 50 53-59 doi: 10.1016/j.placenta.2016.12.024
Allen WR, Gower S, Wilsher S.Implantation and placentation in the mare does not commence until as late as day 40 after ovulation. The reasons for this and the growth factors and/or hormones which drive placentation when it does finally occur are of considerable academic and practical interest. Placental interface tissues recovered from 11 accurately aged and perfused-fixed horse uteri between 20 and 68 days of gestation were stained immunocytochemically for Epidermal Growth Factor (EGF), its specific receptor (EGF-R) and for the steroid hormone enzyme, aromatase. EGF was present in endometrial gland and lumenal epithelia ... Equine hydrallantois is associated with impaired angiogenesis in the placenta.
Placenta
March 5, 2020
Volume 93 101-112 doi: 10.1016/j.placenta.2020.03.001
Dini P, Carossino M, Loynachan AT, El-Sheikh Ali H, Wolfsdorf KE, Scoggin KE, Daels P, Ball BA.Hydrallantois is the excessive accumulation of fluid in the allantoic cavities during the last trimester of pregnancy, leading to abdominal wall hernias, cardiovascular shock, abortion, and dystocia. It has been postulated that hydrallantois is associated with structural and/or functional changes in the chorioallantoic membrane. In the present study, we hypothesized that angiogenesis is impaired in the hydrallantoic placenta. Capillary density in the hydrallantoic placenta was evaluated in the chorioallantois via immunohistochemistry for Von Willebrand Factor. Moreover, the expression of angio... Analysis of MHC class I expression in equine trophoblast cells using in situ hybridization.
Placenta
July 1, 1996
Volume 17, Issue 5-6 351-359 doi: 10.1016/s0143-4004(96)90060-0
Maher JK, Tresnan DB, Deacon S, Hannah L, Antczak DF.Down-regulation of major histocompatibility complex (MHC) genes by trophoblast cells is considered to be a primary mechanism preventing maternal immune rejection of the fetal-placental unit in mammalian pregnancy by rendering these cells, which form the primary barrier between mother and fetus, relatively non-antigenic. In situ hybridization with probes encoding human and horse MHC class I genes was used to characterize the pattern of MHC class I mRNA expression in the various forms of horse trophoblast. Strong hybridization signals were observed in the invasive trophoblast cells of chorionic ... Placental function and structure at term is altered in broodmares fed with cereals from mid-gestation.
Placenta
March 3, 2018
Volume 64 44-52 doi: 10.1016/j.placenta.2018.02.003
Robles M, Peugnet P, Dubois C, Piumi F, Jouneau L, Bouchez O, Aubrière MC, Dahirel M, Aioun J, Wimel L, Couturier-Tarrade A, Chavatte-Palmer P.Feeding pregnant broodmares with cereal concentrates has been shown to increase maternal insulin resistance and affect foal metabolism in the short and long-term. These effects are likely to be mediated by the placenta. Here, we investigated feto-placental biometry and placental structure and function at term in mares fed with or without cereals concentrates. From 7 months of gestation, 22 multiparous mares were fed forage only (group F (n = 12)) or received forage and cracked barley (group B (n = 10)) until foaling. Foals and placentas were weighed and placental samples were collected... Negative uterine asynchrony retards early equine conceptus development and upregulation of placental imprinted genes.
Placenta
July 11, 2017
Volume 57 175-182 doi: 10.1016/j.placenta.2017.07.007
Gibson C, de Ruijter-Villani M, Stout TAE.Placental imprinted genes appear to be sensitive indicators of an inappropriate pre-implantation environment. This study examined the effects of negative uterine asynchrony after embryo transfer (ET) on early horse embryo development, and yolk-sac membrane expression of DNA methyltransferases (DNMTs) and equine specific placental imprinted genes. Methods: Day 8 embryos were transferred to recipient mares on day 8 (synchronous) or day 3 (asynchronous) after ovulation, and conceptuses were recovered 6 or 11 days later (day 14 or 19 of development). Results: Day 14 conceptuses recovered from an a... Alterations in T cell-related transcripts at the feto-maternal interface throughout equine gestation.
Placenta
November 2, 2019
Volume 89 78-87 doi: 10.1016/j.placenta.2019.10.011
Fedorka CE, Loux SL, Scoggin KE, Adams AA, Troedsson MHT, Ball BA.The tolerance of pregnancy by the maternal immune system is balanced between recognition and protection. In the human this is controlled by balancing helper T cell populations (Th1, Th2) in addition to immune suppression from the regulatory arm (Tregs), but this has not been evaluated in the horse. RNA sequencing was performed on chorioallantois and endometrium of mares at 120, 180, 300 and 330 days of gestation (n = 4/stage), as well as 45-day chorioallantois (n = 4) and diestrus endometrium (n = 3). Transcripts were selected for relativity to Th1, Th2, or Treg-associated. qPCR an... Separate sites and mechanisms for placental transport of calcium, iron and glucose in the equine placenta.
Placenta
September 14, 2000
Volume 21, Issue 7 635-645 doi: 10.1053/plac.2000.0550
Wooding FB, Morgan G, Fowden AL, Allen WR.The placenta is the only channel for transport of nutrients to the conceptus and the fetal nutrient demands increase exponentially to term. The 9 kDa calcium binding protein (calbindin, 9CBP) and the iron binding protein uteroferrin (UF) are proving to be reliable markers for epithelia that mediate active transcellular calcium and iron transport and the glucose transporter proteins (GT1 and GT3) for glucose transport by facilitated diffusion. Light and electron microscope immunocytochemistry have been used on perfusion fixed resin embedded material to establish the distribution of 9CBP, UF, GT... Structural and haemovascular aspects of placental growth throughout gestation in young and aged mares.
Placenta
January 9, 2006
Volume 27, Issue 11-12 1103-1113 doi: 10.1016/j.placenta.2005.11.005
Abd-Elnaeim MM, Leiser R, Wilsher S, Allen WR.This investigation was carried out to study the equine placenta from early gestation to near term, with special reference to morphological changes associated with the development of the vasculature of the fetal component of the microcotyledons. Pregnant uteri were removed post mortem from five Thoroughbred mares between 110 and 309 days of gestation, two of which were aged, multiparous animals suffering age-related degenerative changes in their endometrium (endometrosis), while the other three were young, and had primigravid normal healthy uteri. Pieces of endometrium with placenta attached we... The potential of collagenase as a new therapy for separation of human retained placenta: hydrolytic potency on human, equine and bovine placentae.
Placenta
August 12, 1998
Volume 19, Issue 5-6 379-383 doi: 10.1016/s0143-4004(98)90077-7
Fecteau KA, Haffner JC, Eiler H.The purpose of this study was to determine to what degree bacterial collagenase may digest human placentae compared to equine and bovine placentae. Placenta samples from human, equine and bovine were incubated with bacterial collagenase solution at various concentrations. The degree of hydrolysis and collagen breakdown was measured by the release of total proteins and hydroxyproline into the incubation media. Also, whole placentae were injected via umbilical cord arteries with collagenase solution (200 U/ml, 200 ml total volume in human and 1000 ml in equine) and hydrolysis determined chemical... Differences in extracellular matrix remodeling in the placenta of mares that retain fetal membranes and mares that deliver fetal membranes physiologically.
Placenta
August 7, 2015
Volume 36, Issue 10 1167-1177 doi: 10.1016/j.placenta.2015.07.126
Rapacz-Leonard A, Kankofer M, Leonard M, Wawrzykowski J, Dąbrowska M, Raś A, Paździor-Czapula K, Janowski T.In mammals, placenta separation at term may involve degradation of the extracellular matrix by matrix metalloproteinases (MMPs). The activity of MMPs is modulated by TIMPs. We hypothesized that the placentas of mares that deliver fetal membranes physiologically and those that retain fetal membranes (FMR) differ in terms of histology; mRNA expression of MMP-2 and MMP-9; protein expression of MMP-2, MMP-9, and TIMP-2; and the potential activity of both MMPs. Methods: Placenta biopsies were taken from mares (n = 9; 4 FMR, 5 controls) immediately after foal expulsion. Retention was defined as fail... Major histocompatibility complex class I in the horse (Equus caballus) placenta during pregnancy and parturition.
Placenta
December 24, 2018
Volume 74 36-46 doi: 10.1016/j.placenta.2018.12.006
Rapacz-Leonard A, Leonard M, Chmielewska-Krzesińska M, Paździor-Czapula K, Janowski T.Major histocompatibility protein class I (MHC-I) is believed to be expressed in the horse allantochorion only in limited areas at limited times. However, its expression has only been investigated in early pregnancy with non-quantitative techniques that cannot reliably detect small amounts of protein. To quantify the relative expression of MHC-I in the allantochorion and endometrium during days 90-240 of pregnancy (PREG), parturition with physiological delivery of fetal membranes (PHYS), and parturition with retention of these membranes (FMR). Also, to visualize protein expression and determine... The proteome of fetal fluids in mares with experimentally-induced placentitis.
Placenta
March 20, 2018
Volume 64 71-78 doi: 10.1016/j.placenta.2018.03.004
Loux SC, Ball BA.Placental inflammation (placentitis) is one of the leading causes of late-term abortion in mares. Although prognosis is good assuming early diagnosis and treatment, diagnostics are limited. To better characterize the disease and identify potential biomarkers, we analyzed the proteome of fetal fluids (amniotic and allantoic) in both control mares (n = 5) and mares with experimentally-induced placentitis (n = 5) using LTQ-Orbitrap mass-spectrometry. Placentitis was induced via trans-cervical inoculation of Streptococcus equi ssp. zooepidemicus. In total, 130 proteins were identified in e... A lectin histochemical study to detect variation in glycosylation at the feto-maternal interface in three interbreeding equine species.
Placenta
September 4, 2017
Volume 58 115-121 doi: 10.1016/j.placenta.2017.08.075
Jones CJP, Allen WRT, Wilsher S.In this study, we compare glycosylation at the fetomaternal interface in 3 equine species: horse, donkey and zebra, all of which can interbreed to produce hybrids, to assess their glycan similarities and differences. Methods: Sections cut from 3 specimens of horse (Equus caballus) placenta (50, 200 and 280 days gestation), one donkey (Equus asinus) placenta (65 cm crown-rump length) and 5 specimens of zebra (Equus quagga) placentae (81-239 days gestation) were stained with a panel of 24 biotinylated lectins using an avidin-peroxidase revealing system. Results: There were only slight quantitat... Absence of maternal cell contamination in mesenchymal stromal cell cultures derived from equine umbilical cord tissue.
Placenta
May 20, 2014
Volume 35, Issue 8 655-657 doi: 10.1016/j.placenta.2014.04.005
Vackova I, Czernekova V, Tomanek M, Navratil J, Mosko T, Novakova Z.This study aimed to determine whether maternal cell contamination exists in cells derived from equine umbilical cord tissue, a perspective material for cell-based therapies in veterinary medicine. Potential maternal cell contamination was analyzed at DNA level via a set of 16 microsatellite markers in cells originating from the cord tissue of 22 foals. In these cells no maternal cell contamination was detected at a sensitivity level of 0.01%. Our results suggest that equine umbilical cord tissue-derived cells are entirely of fetal origin. What fossils can tell us about the evolution of viviparity and placentation.
Placenta
September 6, 2008
Volume 29, Issue 11 930-931 doi: 10.1016/j.placenta.2008.07.012
Carter AM.Recently a fossil of one of the earliest jawed fishes was found with a fetal skeleton and the remains of a cord. It was from the Devonian period and takes the history of vertebrate placentation back to 380 million years ago. This and later fossil evidence for viviparity in marine reptiles and early mammals is reviewed. Of particular interest are the fossils of horses as they document that a reproductive strategy with a single precocial newborn was evolved early on. In one instance there is sufficient representation of soft tissue to imply that early horses had a diffuse placenta, much as had b... Scanning electron microscopy of the microcotyledonary placenta of the horse (Equus caballus) in the latter half of gestation.
Placenta
August 15, 2000
Volume 21, Issue 5-6 565-574 doi: 10.1053/plac.2000.0510
Macdonald AA, Chavatte P, Fowden AL.Scanning electron microscopy was used to examine the microstructure of the maternal and fetal placenta from 25 pregnant ponies (Equus caballus) throughout the second half of gestation. Samples of placenta, 2-6 cm(2)in area, were collected from the antimesometrial region of the pregnant horn at 105 days (n=1), 165-219 days (n=5), 260 days (n=3), 270-277 days (n=3), 313-337 days (n=11) and immediately after spontaneous delivery at term (n=2). The maternal microcaruncle appears to be created from a clustering of about 16 uterine crypts encapsulated in a connective tissue sheath. There is a gestat... A lectin binding analysis of glycosylation patterns during development of the equine placenta.
Placenta
February 9, 1999
Volume 20, Issue 1 45-57 doi: 10.1053/plac.1998.0354
Jones CJ, Wooding FB, Dantzer V, Leiser R, Stoddart RW.The glycosylation of the equine interhaemal barrier and areola was studied throughout the period of gestation. Placentae of 35, 37, 50, 119, 152, 200, 280 and 300 days gestation were investigated, using semithin plastic embedded sections and a panel of 15 biotinylated lectins with an avidin-peroxidase revealing system. Glycosylation of the trophoblast and maternal epithelium showed the most change during the first 50 days of gestation, being associated with the initial stages of adhesion and attachment. In the trophoblast, non-bisected tri/tetraantennary complex N-glycan was only evident after... A method for isolating and culturing placental cells from failed early equine pregnancies.
Placenta
December 24, 2015
Volume 38 107-111 doi: 10.1016/j.placenta.2015.12.014
Rose BV, Cabrera-Sharp V, Firth MJ, Barrelet FE, Bate S, Cameron IJ, Crabtree JR, Crowhurst J, McGladdery AJ, Neal H, Pynn J, Pynn OD, Smith C....Early pregnancy loss occurs in 6-10% of equine pregnancies making it the main cause of reproductive wastage. Despite this, reasons for the losses are known in only 16% of cases. Lack of viable conceptus material has inhibited investigations of many potential genetic and pathological causes. We present a method for isolating and culturing placental cells from failed early equine pregnancies. Trophoblast cells from 18/30 (60%) failed equine pregnancies of gestational ages 14-65 days were successfully cultured in three different media, with the greatest growth achieved for cells cultured in Amnio... Placental localization of relaxin in the pregnant mare.
Placenta
March 1, 1997
Volume 18, Issue 2-3 121-128 doi: 10.1016/s0143-4004(97)90083-7
Klonisch T, Mathias S, Cambridge G, Hombach-Klonisch S, Ryan PL, Allen WR.In situ hybridization employing a cRNA probe derived from a 428-bp fragment of equine relaxin was used to localize relaxin mRNA, and immunocytochemistry was used to localize relaxin itself, in tissues of the placenta-endometrium interface recovered between 33 and 153 days of gestation from mares carrying intraspecific horse, interspecific mule and extraspecific donkey conceptuses. Immunocytochemical staining was also used to localize trophoblast-specific and class I major histocompatibility complex (MHC) antigens on some specimens. Relaxin mRNA and relaxin were both present in the single-cell ... Release of lipid from the equine placenta during in vitro incubation.
Placenta
December 1, 1994
Volume 15, Issue 8 857-872 doi: 10.1016/s0143-4004(05)80187-0
Stammers JP, Hull D, Silver M, Fowden AL, Ousey J, Rossdale PD.An in vitro incubation technique was used to examine release of lipids from the equine placenta. Placental tissue was obtained at term (n = 5, term = 320-365 days) and earlier in gestation (n = 8, mean = 266 days). Term placentae were incubated at two temperatures, 4 degrees C (control) and 37 degrees C for 2 h. Pre-term placentae were incubated at 37 degrees C with two different concentrations of fatty acid in the medium. Tissues and media were analysed for their lipid concentrations. Term and pre-term placentae released free fatty acid (FFA) and phospholipid into the incubation medium during... Equine placental cup cells show glycan expression distinct from that of both chorionic girdle progenitor cells and early allantochorionic trophoblast of the placenta.
Placenta
May 18, 1999
Volume 20, Issue 4 347-360 doi: 10.1053/plac.1998.0388
Jones CJ, Enders AC, Wooding FP, Dantzer V, Leiser R, Stoddart RW.Using lectin histochemistry on plastic-embedded material, the glycosylation patterns of equine girdle and cup cells, and associated endometrial glands, have been investigated from 37 to 67 days gestation. Results were compared with the glycosylation of the 50-day allantochorionic trophoblast of the established equine placenta that will later form the microcotyledons. The differentiated cup cells, which secrete equine chorionic gonadotropin (eCG), showed a pattern of glycosylation that was distinct both from the progenitor girdle cells and the allantochorionic trophoblast, with granules that bo... Equine endometrium at pre-implantation stages of pregnancy has specific glycosylated regions.
Placenta
November 1, 1985
Volume 6, Issue 6 537-542 doi: 10.1016/s0143-4004(85)80007-2
Whyte A, Allen WR.Pre- and post-implantation endometrial tissue was removed from equids at known stages of pregnancy. Thin sections of this material were reacted with fluorescein conjugates of several lectins. Two lectins in particular, with specificities for L-fucose and N-acetylglucosamine, reacted strongly with the endometrial epithelium. The reaction for N-acetylglucosamine occurred in small patches on the endometrial epithelium and extended to cover the entire epithelial surface and endometrial glands after implantation. L-Fucose was observed in larger surface deposits particularly at the openings of endom...