Prostaglandins are lipid compounds derived from fatty acids that are present in various tissues of horses. They are involved in a wide range of physiological processes, including the regulation of inflammation, blood flow, and reproductive functions. In equine medicine, prostaglandins are often studied for their role in modulating inflammatory responses and their potential therapeutic applications. These compounds can influence pain perception and are involved in processes such as parturition and the estrous cycle in mares. This page collects peer-reviewed research studies and scholarly articles that explore the synthesis, function, and clinical applications of prostaglandins in equine health.
Allen WR, Stewart F, Cooper MJ, Crowhurst RC, Simpson DJ, McEnery RJ, Greenwood RE, Rossdale PD, Ricketts SW.This research investigates the use of synthetic prostaglandin analogues, specifically ICI-81008 and ICI-79939, in mares for inducing luteolysis, the regression of the corpus luteum, to manage infertility issues. The study […]
Shand N, Irvine CH, Turner JE, Alexander SL.Jugular blood samples were collected at 4 h intervals from six mares during an oestrous cycle to study the hormonal events that occur around the time of luteolysis. Blood samples from day 10 (day 0 = ovulation) until day 3 of oestrus were assayed for prostaglandin metabolite 13,14-dihydro-15-keto PGF2alpha (PGFM), oxytocin, prolactin, progesterone and oestrogen conjugates. PGF2alpha (0.5 or 1.5 mg) was administered to six mid-dioestrous mares and the oxytocin and prolactin responses were measured. One to five large (peak > or =2 x nadir) pulses of PGFM, oxytocin and prolactin were detected ...
Hinrichs K, Riera FL.Ten mares were used to investigate the effect of administration of prostaglandin F2 alpha on uterine tubal motility, as reflected by embryo recovery from the uterus 5 days after ovulation (day 0). Mares were assigned to 3 groups: group A, uterine flush for embryo recovery on day 7; group B, uterine flush for embryo recovery on day 5; and group C, uterine flush for embryo recovery on day 5, after treatment with prostaglandin F2 alpha (10 mg, IM) on day 3. Each mare was assigned to each group once. Embryo recovery rates for the 3 groups were: A, 6 of 10; B, 2 of 8; and C, 0 of 10. The embryo rec...
Roth TL, White KL, Thompson DL, Horohov DW.It has been proposed that PGE2 is an important immunosuppressant acting at the fetal-maternal interface during pregnancy. We have previously shown that horse conceptus-conditioned medium suppresses lymphocyte proliferation. This experiment was designed to determine if horse conceptus-derived immunosuppressive activity could be attributed to PGE2 production by the trophoblast tissue. Trophoblast tissue from 21-day-old conceptuses was cut into equal sections and cultured in the presence or absence of the prostaglandin inhibitor, indomethacin. Following culture, immunosuppressive activity and the...
Alkhadrawy JMH, Aboelmaaty AM, Abou-Ahmed MM, Ghallab AM.Commercial embryo flushing of horses has required hormonal management of both the donor and recipient mares throughout the breeding season. Unassigned: This study aimed to find out the effect of using human chorionic gonadotropin (hCG) and prostaglandin F2α (PG) on the ovarian and uterine dynamics and hemodynamics, estradiol (E2), progesterone, oxidants-antioxidants, and blood biochemicals in embryo donor mares during the hottest months of the year in a subtropical climate. Unassigned: Three Control estrous cycles of native mares (10-20 years; = 10) followed by two treated cycles with hCG an...
Amaral A, Cerveira-Pinto M, Santos C, Kordowitzki P, Skarzynski D, Ferreira-Dias G, Szóstek-Mioduchowska A.Transforming growth factor (TGF)-β1 is a pro-fibrotic cytokine that affects extracellular matrix (ECM) deposition and fibroblast activity. 17β-Estradiol (E), the dominant ovarian steroid during the follicular phase (FLP) of the estrous cycle, can also influence ECM remodeling and fibrosis, through prostaglandin (PG) synthesis. PGs have opposing roles in fibrosis, with PGE₂ showing anti-fibrotic effects and PGF₂α promoting fibrosis. Equine endometrosis, whose main pathological feature is fibrosis, is marked by chronic inflammation and ECM accumulation, and may involve mediators like TGF-...
Gaber JTH, Cuervo-Arango J, Plaza-Dávila M, Martínez-Boví R.This experiment was performed to evaluate whether intrafollicular treatment of PGE2 or PGF2α administered in early estrus would induce normal ovulation, progesterone production (Experiment 1) and pregnancy (Experiment 2). In Experiment 1, mares in estrus after 2 days of endometrial edema were injected in all largest dominant follicles (28-35 mm in diameter) with 0.5 mL of sterile water containing 500 μg PGE2 (n = 6), 125 μg PGF2α (n = 6) or placebo (n = 7) (Hour 0). Ultrasound examinations were performed daily, until ovulation or anovulation was detected, and daily blood samples ...
Cuervo-Arango J.The objective of this study was to compare the fertility of diestrous mares with a follicle ≥ 30 mm in diameter treated with prostaglandin F2α (PGF) (PGF group) or left untreated for 7 days (SP group). Standardbred mares (n = 46) were assigned randomly to the SP (n = 23) and PGF groups (n = 23) when they were identified with a corpus luteum (CL) of unknown age and a follicle ≥ 30 mm in diameter (Day 0). The most frequent outcome in the PGF group was estrus and ovulation of the dominant follicle (74 %), while the outcomes of the SP mares 7 days after Day 0 were more variable: estrus and fo...
Sadowska A, Wójtowicz A, Molcan T, Drzewiecka EM, Kaczmarek MM, Słyszewska M, Ferreira-Dias G, Szóstek-Mioduchowska A.Equine endometrosis is a chronic degenerative condition with fibrosis being one of the most significant characteristics. A growing body of evidence indicates the critical role of interleukin (IL)-17 in fibrotic disorders. However, its exact role during equine endometrosis remains to be discovered and explained. The main aim of the current study was to establish the expression of IL-17A signaling components in equine endometria with and without endometrosis as well as the effects of IL-17A on the transcriptomic signature, cellular functional characteristics, expression of extracellular matrix (...
Pinto-Bravo P, Rebordão MR, Amaral AS, Szóstek-Mioduchowska A, Fernandes C, Galvão AM, Silva E, Alpoim-Moreira J, da Costa RPR, Skarzynski DJ....Oviduct glycoprotein 1 (OVGP1) is a key protein involved in oviductal functions. β-estradiol (E) and progesterone (P), oxytocin (OXT) and tumor necrosis factor-α (TNFα) modulate the equine oviduct function, through prostaglandin regulation. The objective was to evaluate OVGP1 expression within each equine oviduct segment (infundibulum, ampulla isthmus), throughout the estrous cycle. The in vitro effect of (i) E, P, OXT, TNFα; and (ii) spermatozoa, on oviduct OVGP1 transcription and secretion was studied. Gene transcription was assessed by real-time PCR; protein expression by western blot; ...
Del Prete C, Gaspari G, Kosior MA, Merlo B, Iacono E, Longobardi C, Martino NA, Dell'Aquila ME, Damiano S, Cocchia N, Gasparrini B, Lange-Consiglio A.Over the years, regenerative therapies have emerged as promising alternatives for persistent breeding-induced endometritis. In vitro studies testing the effects of these therapies on equine endometrial cells are still scarce. This study aimed to evaluate in vitro the effect of Wharton's jelly (WJ) mesenchymal stromal/stem cell (MSCs)-derived conditioned medium (WJ-CM) and platelet-rich plasma (PRP) on equine endometrial cells, with or without lipopolysaccharide (LPS)-induced inflammation. The WJ-CM was obtained after 24 h of starvation in Ringer's lactate of WJ-MSCs and PRP was prepared using...
