Virology in horses encompasses the study of viruses that affect equine species, including their biology, transmission, and impact on horse health. This field investigates viral pathogens that can lead to a range of diseases, from respiratory infections to neurological disorders. Common viruses affecting horses include equine influenza virus, equine herpesvirus, and West Nile virus. Understanding these viruses involves examining their genetic makeup, modes of transmission, and interactions with the equine immune system. This page compiles peer-reviewed research studies and scholarly articles that explore the epidemiology, pathogenesis, and control measures of viral infections in horses.
Marois P, Boudreault A, DiFranco E, Pavilanis V.Rhesus monkeys and ferrets were exposed to intranasal inoculation of several strains of egg-adapted avian, equine and human influenza viruses and to strains of mouse-adapted equine influenza viruses. Local replication of virus and seroconversion were observed in the majority of these animals. However, clinical infection was observed only in ferrets.
Schmidt NJ, Melnick JL, Wenner HA, Ho HH, Burkhardt MA.Immune horse sera to 42 enterovirus immunotypes were pooled according to the Lim Benyesh-Melnick and the "intersecting serum" schemes. Each serum was diluted in the pools to contain 50 antibody units. After it was established that the pools correctly neutralized prototype virus strains, they were evaluated in tests against 273 enterovirus field strains representing most of the viral types included in the pools. With test virus doses of 10-100 TCD(50), most of the poliovirus and coxsackievirus field strains were correctly identified in both schemes, but a number of the echoviruses were neutrali...
Scott GR.Twelve DNA viruses and forty‐three RNA viruses are known to infect horses. In addition, there are three unclassified viruses and, at least, three alleged viruses infecting horses. Differential diagnosis is difficult. At least twenty‐eight of the fifty‐eight viruses induce clinical disease but the range of syndromes is limited; eleven provoke respiratory symptoms and eleven cause encephalitis. Thirty‐four equine viruses with a limited geographical distribution are transmitted by arthropod vectors. Twenty viruses are spread by contact and their distribution, in general, is global. The ve...
Moore RW, Redmond HE, Katada M, Wallace M.A continuous-passage horse leukocyte culture [V.B. 40, abst. 4672] was susceptible to the virus of equine infectious anaemia, as determined by cyto-pathic effect and viral titre. Ultrafiltration studies indicated that the virus was less than 32 mμ in diameter, which agrees with previous reports. Susceptible horses developed clinical signs and lesions of the disease when they were inoculated with both unfiltered and ultrafiltered culture virus.
Ley KD, Burger D, McGuire T, Henson JB.The role of interferon in the pathogenesis of per-
sistent or chronic viral diseases has not been elu-
cidated. However, interferon or inducers of inter-
feron may have a marked effect on the course of
certain chronic viral diseases [1-3]. Equine infec-
tious anemia (EIA) is a viral disease of horses
that may take an inapparent, acute, subacute, or
chronic course in the affected host [4]. Chronically
infected horses suffer from recurrent attacks of
fever that are accompanied by severe anemia.
After the infection has been established, infected
horses may carry the virus for the rest ...
Fulton RE, Doane FW, Macpherson LW.Combined light and electron microscopy were used to follow the sequence of virus development in equine papillomas. The deepest layer in which virus was observed was the stratum spinosum of the epidermis. In this layer virus was scattered throughout the nuclei and was occasionally found in association with the nucleolus. In the stratum granulosum virus particles were more numerous, often forming isolated nuclear aggregates. Virus inclusions observed in the stratum granulosum by electron microscopy were correlated with nuclear inclusions seen by light microscopy. In the stratum corneum closely p...
Rhim JS, Ro HS, Kim EB, Gilden RV, Huebner RJ.A horse skin cell line (E. Derm, NBL-6, CCL-57) was susceptible to focus formation by the Kirsten mouse sarcoma virus, feline sarcoma virus (ST stain) and the MSV pseudotypes with woolly monkey, gibbon monkey, RD-114, AT-124, baboon placenta and murine xenotropic (BALB/c 3T3 and C57L/JD) type-C viruses. Foci were detected within 5 days after infection and the transformed cells continued to produce infectious virus and group-specific antigen of their respective type-C leukemia viruses. The transformation efficiency of various type-C sarcoma viruses in horse cells was also very high.
Horenstein AL, Glait HM, Koss A.A monoclonal anti-equine infectious anemia virus (anti-EIAV) antibody (1B15) has been generated by fusion of X63 Ag 8.653 myeloma cells and spleen cells from mice hypersensitized with viral antigen p29. Ouchterlony double-diffusion analysis indicated that antibody 1B15 is of the IgG class. The specificity of the immune reaction for p29 was confirmed by cross-over immunoelectrophoresis and disc-gel electrophoresis. MAb 1B15 was used to devise a solid-phase 'capture' RIA for EIAV-p29 antigen. The antigen, bound by 1B15 adsorbed onto wells of flexible microtitre plates, was detected using a rabbi...
Balasuriya UBR.The primary goals of this chapter are to discuss common viral RNA isolation and purification methods that are routinely used by various diagnostic laboratories and to highlight the advantages and drawbacks of each method and to identify the most suitable and reliable method to increase the sensitivity and specificity of RT-PCR assays for the detection of equine influenza virus (EIV) in clinical specimens. Our experiences and review of literature show that magnetic bead-based nucleic extraction methods (manual and automatic) work well for isolation and purification of EIV RNA from nasal swab sp...
Crucière C, Guillemin MC, Roseto A, Wirbel A, Plateau E.Monoclonal antibodies (Mo Abs) were prepared against influenza/A/equine/Prague/1/56 (H7N7) and influenza/A/equine/Miami/1/63 (H3N8) reference strains of equine influenza virus. These monoclonals were tested against the 2 reference strains, 8 field strains of equine influenza virus, 3 human influenza viruses possessing the H3 hemagglutinin, and one virus of human origin possessing the H1 hemagglutinin. Two antibodies were obtained in one fusion against the Prague/1/56 strain and reacted only with this strain. Four anti/A/equine/Miami/1/63 Mo Abs were obtained in one fusion. They differentiated ...