FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Cells2023; 12(2); 320; doi: 10.3390/cells12020320

A Combination of Taurine and Caffeine in Stallion Semen Extender Positively Affects the Spermatozoa Parameters.

Abstract: This study was aimed to determine the impact of different taurine and caffeine combinations on the motility, viability, and oxidative markers of chilled stallion spermatozoa. Each stallion semen sample was diluted in a ratio of 1:2, with various taurine and caffeine concentrations (2.5-7.5 mg/mL taurine + 0.625-1.25 mg/mL caffeine) dissolved in a conventional extender. The control samples (CON) were prepared by diluting ejaculate only using the conventional extender. The motility was analyzed using a CASA system at different time intervals (0, 6, 12, 24, and 30 h) and the viability was evaluated using a mitochondrial toxicity test (MTT) performed at the end of the incubation at 5 ยฐC. The liquid part of experimental samples was separated by centrifugation after 30 h of incubation and underwent the evaluation of oxidative stress via the quantification of markers ferric reducing ability of plasma (FRAP) and total oxidant status (TOS). The samples that were treated with a combination of taurine and caffeine significantly improved the motility parameters, mainly after 12, 24, and 30 h of incubation. Samples extended with combination of taurine and caffeine neither compromise viability nor alterations of redox status. The results of this study describe the combination of taurine and caffeine as an optimal supplement for improving the quality of stallion semen during chilled storage.
Get Full Text
Publication Date: 2023-01-14 PubMed ID: 36672253PubMed Central: PMC9856288DOI: 10.3390/cells12020320Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study investigated the effects of taurine and caffeine on the viability, motility, and oxidative markers of chilled stallion sperm. The findings suggested that a combination of these two substances can significantly improve the quality of stallion semen during chilled storage.

Research Methodology

  • The researchers set out to determine the impact of different combinations of taurine and caffeine on the motility, viability, and oxidative markers of chilled stallion spermatozoa.
  • The sperm samples from stallions were diluted in a ratio of 1:2. The dilution agent consisted of various concentrations of taurine and caffeine (ranging from 2.5-7.5 mg/mL for taurine and 0.625-1.25 mg/mL for caffeine) dissolved in a conventional semen extender.
  • Control samples were also prepared by simply diluting the ejaculate with the conventional extender only.

Analysis and Evaluation

  • The mobility of the sperm samples was analyzed using a Computer-Assisted Sperm Analyses (CASA) system at fixed time intervals, which were 0, 6, 12, 24, and 30 hours respectively.
  • The viability of the sperm was evaluated using a mitochondrial toxicity test (MTT) conducted at the end of the incubation period at a temperature of 5 degrees Celsius.
  • The liquid portion of the experimental samples was separated by centrifugation after 30 hours of incubation to assess oxidative stress. This assessment was carried out through the quantification of oxidative markers known as Ferric Reducing Ability of Plasma (FRAP) and Total Oxidant Status (TOS).

Findings

  • The study revealed a significant improvement in the motility parameters of the sperm samples that were treated with a combination of taurine and caffeine, particularly after 12, 24, and 30 hours of incubation.
  • No compromise in viability or alterations in redox status were observed in samples extended with a combination of taurine and caffeine.
  • Overall, the study demonstrated that taurine and caffeine as combined supplements provide an optimum solution for improving the quality of stallion semen during chilled storage.

Cite This Article

APA
Halo M, Tirpรกk F, Slanina T, Tokรกrovรก K, Massรกnyi M, Dianovรก L, Mlynekovรก E, Greล„ A, Halo M, Massรกnyi P. (2023). A Combination of Taurine and Caffeine in Stallion Semen Extender Positively Affects the Spermatozoa Parameters. Cells, 12(2), 320. https://doi.org/10.3390/cells12020320

Publication

Cells
ISSN: 2073-4409
NlmUniqueID: 101600052
Country: Switzerland
Language: English
Volume: 12
Issue: 2
PII: 320

Researcher Affiliations

Halo, Marko
  • Institute of Applied Biology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.
Tirpรกk, Filip
  • AgroBioTech, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.
Slanina, Tomรกลก
  • Institute of Applied Biology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.
Tokรกrovรก, Katarรญna
  • Institute of Applied Biology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.
Massรกnyi, Martin
  • AgroBioTech, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.
Dianovรก, Lucia
  • Institute of Applied Biology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.
Mlynekovรก, Eva
  • Institute of Animal Husbandry, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.
Greล„, Agnieszka
  • Institute of Biology, Pedagogical University of Krakow, Podchorazych 2, 30-084 Krakow, Poland.
Halo, Marko
  • Institute of Animal Husbandry, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.
Massรกnyi, Peter
  • Institute of Applied Biology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.

MeSH Terms

  • Animals
  • Horses
  • Male
  • Semen
  • Caffeine / pharmacology
  • Taurine / pharmacology
  • Sperm Motility
  • Semen Preservation / veterinary
  • Semen Preservation / methods
  • Spermatozoa

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 35 references
  1. Halo M, Tirpรกk F, Kovรกฤik A, Lรญpovรก P, Greล„ A, Massรกnyi P. Biochemical parameters of seminal plasma affect motility traits of stallion spermatozoa.. J. Microbiol. Biotechnol. Food Sci. 2018;7:516โ€“518.
    doi: 10.15414/jmbfs.2018.7.5.472-474google scholar: lookup
  2. Katila T. Procedures for handling fresh stallion semen.. Theriogenology 1997 Nov;48(7):1217-27.
    doi: 10.1016/S0093-691X(97)00354-3pubmed: 16728210google scholar: lookup
  3. Batellier F, Vidament M, Fauquant J, Duchamp G, Arnaud G, Yvon JM, Magistrini M. Advances in cooled semen technology.. Anim Reprod Sci 2001 Dec 3;68(3-4):181-90.
    doi: 10.1016/S0378-4320(01)00155-5pubmed: 11744263google scholar: lookup
  4. Aurich C. Recent advances in cooled-semen technology.. Anim Reprod Sci 2008 Sep;107(3-4):268-75.
    doi: 10.1016/j.anireprosci.2008.04.015pubmed: 18524507google scholar: lookup
  5. Partyka A, Niลผaล„ski W. Supplementation of Avian Semen Extenders with Antioxidants to Improve Semen Quality-Is It an Effective Strategy?. Antioxidants (Basel) 2021 Nov 30;10(12).
    doi: 10.3390/antiox10121927pmc: PMC8750219pubmed: 34943030google scholar: lookup
  6. Halo M, Massรกnyi M, Tokรกrovรก K, Tirpรกk F, Greifovรก H, Solรกr D, Halo M, Massรกnyi P. High taurine concentrations negatively effect stllion spermatozoa parameters in vitro.. Acta Fytotech. Zootech. 2021;24:15โ€“19.
    doi: 10.15414/afz.2021.24.mi-prap.15-19google scholar: lookup
  7. Tirpรกk F, Slanina T, Ofรบkanรฝ M, Lukรกฤ N, Massรกnyi P. Effect of taurine on bovine spermatozoa motility parameters following cryopres.ervation.. Slovak J. Anim. Sci. 2015;48:49โ€“56.
  8. Jang H.Y, Kong H.S, Park C.K, Oh J.D, Lee S.G, Cheong H.T, Kim J.T, Lee S.J, Yang B.K, Lee H.K. Effects of Taurine on Sperm Characteristics during In vitro Storage of Boar Semen.. Asian-Australas. J. Anim. Sci. 2006;19:1561โ€“1565.
    doi: 10.5713/ajas.2006.1561google scholar: lookup
  9. Shiva Shankar Reddy N, Jagan Mohanarao G, Atreja SK. Effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo (Bubalus bubalis) sperm quality following cryopreservation.. Anim Reprod Sci 2010 Jun;119(3-4):183-90.
    doi: 10.1016/j.anireprosci.2010.01.012pubmed: 20197223google scholar: lookup
  10. Martins-Bessa A, Rocha A, Mayenco-Aguirre A. Effects of taurine and hypotaurine supplementation and ionophore concentrations on post-thaw acrosome reaction of dog spermatozoa.. Theriogenology 2009 Jan 15;71(2):248-53.
    doi: 10.1016/j.theriogenology.2008.07.006pubmed: 18774600google scholar: lookup
  11. Tirpรกk F, Slanina T, Kovรกฤik A, Ondruลกka L, Massรกnyi P, Halo M, Massรกnyi P. Low taurine concentrations possitively affect rabbit spermatozoa properties in later time intervals.. J. Microbiol. Biotechnol. Food Sci. 2017;7:128โ€“131.
    doi: 10.15414/jmbfs.2017.7.2.128-131google scholar: lookup
  12. Slanina T, Miลกkeje M, Tirpรกk F, Baszczyk M, Stawarz R, Massรกnyi P. Effect of taurine on Turkey (Meleagris gallopavo) spermatozoa viability and motility.. Czech J. Anim. Sci. 2018;63:127โ€“135.
    doi: 10.17221/79/2017-CJASgoogle scholar: lookup
  13. Perumal P, Vupru K, Rajkhowa C. Effect of Addition of Taurine on the Liquid Storage (5ยฐC) of Mithun (Bos frontalis) Semen.. Vet Med Int 2013;2013:165348.
    doi: 10.1155/2013/165348pmc: PMC3703388pubmed: 23853737google scholar: lookup
  14. Stephens T.D, Brooks R.M, Carrington J.L, Cheng L, Carrington A.C, Porr C.A, Splan R.K. Effects of pentoxifylline, caffeine, and taurine on post-thaw motility and longevity of equine frozen semen.. J. Equine. Vet. Sci. 2013;33:615โ€“621.
    doi: 10.1016/j.jevs.2012.10.004google scholar: lookup
  15. Azam S, Hadi N, Khan NU, Hadi SM. Antioxidant and prooxidant properties of caffeine, theobromine and xanthine.. Med Sci Monit 2003 Sep;9(9):BR325-30.
    pubmed: 12960921
  16. Halo M, Tirpรกk F, Massรกnyi M, Kovรกฤ J, Mlynekovรก E, Greล„ A, Halo M, Massรกnyi P. The effects of caffeine on the motility and viability of stallion spermatozoa at different temperature conditions.. Acta Vet Hung 2022 Jun 16;.
    doi: 10.1556/004.2022.00016pubmed: 35895556google scholar: lookup
  17. Funahashi H, Nagai T. Regulation of in vitro penetration of frozen-thawed boar spermatozoa by caffeine and adenosine.. Mol Reprod Dev 2001 Apr;58(4):424-31.
    doi: 10.1002/1098-2795(20010401)58:4<424::AID-MRD10>3.0.CO;2-1pubmed: 11241779google scholar: lookup
  18. Slanina T, Miลกkeje M, Petroviฤovรก I, Massรกnyi P. The influence of caffeine on turkey spermatozoa motility during in vitro cultivation at 41 ยฐC.. J. Microbiol. Biotechnol. Food Sci. 2014;3:157โ€“159.
  19. Pereira RJ, Tuli RK, Wallenhorst S, Holtz W. The effect of heparin, caffeine and calcium ionophore A23187 on in vitro induction of the acrosome reaction in frozen-thawed bovine and caprine spermatozoa.. Theriogenology 2000 Jul 15;54(2):185-92.
    doi: 10.1016/S0093-691X(00)00340-Xpubmed: 11003300google scholar: lookup
  20. Tirpรกk F, Halo M Jr, Tokรกrovรก K, Binkowski LJ, Vaลกรญฤek J, Svoradovรก A, Bล‚aszczyk-Altman M, Kovรกฤik A, Tvrdรก E, Chrenek P, Lukรกฤ N, Massรกnyi P. Composition of Stallion Seminal Plasma and Its Impact on Oxidative Stress Markers and Spermatozoa Quality.. Life (Basel) 2021 Nov 16;11(11).
    doi: 10.3390/life11111238pmc: PMC8624310pubmed: 34833114google scholar: lookup
  21. Tirpak F, Slanina T, Tomka M, Zidek R, Halo M Jr, Ivanic P, Gren A, Formicki G, Stachanczyk K, Lukac N, Massanyi P. Exposure to non-ionizing electromagnetic radiation of public risk prevention instruments threatens the quality of spermatozoids.. Reprod Domest Anim 2019 Feb;54(2):150-159.
    doi: 10.1111/rda.13338pubmed: 30192989google scholar: lookup
  22. Halo M Jr, Buล‚ka K, Antos PA, Greล„ A, Slanina T, Ondruลกka ฤฝ, Tokรกrovรก K, Massรกnyi M, Formicki G, Halo M, Massรกnyi P. The effect of ZnO nanoparticles on rabbit spermatozoa motility and viability parameters in vitro.. Saudi J Biol Sci 2021 Dec;28(12):7450-7454.
    doi: 10.1016/j.sjbs.2021.08.045pmc: PMC8626300pubmed: 34867049google scholar: lookup
  23. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay.. Anal Biochem 1996 Jul 15;239(1):70-6.
    doi: 10.1006/abio.1996.0292pubmed: 8660627google scholar: lookup
  24. Erel O. A new automated colorimetric method for measuring total oxidant status.. Clin Biochem 2005 Dec;38(12):1103-11.
    doi: 10.1016/j.clinbiochem.2005.08.008pubmed: 16214125google scholar: lookup
  25. Sun Y, Dai S, Tao J, Li Y, He Z, Liu Q, Zhao J, Deng Y, Kang J, Zhang X, Yang S, Liu Y. Taurine suppresses ROS-dependent autophagy via activating Akt/mTOR signaling pathway in calcium oxalate crystals-induced renal tubular epithelial cell injury.. Aging (Albany NY) 2020 Sep 15;12(17):17353-17366.
    doi: 10.18632/aging.103730pmc: PMC7521519pubmed: 32931452google scholar: lookup
  26. Tvrdรก E, Kลˆaลพickรก Z, Bรกrdos L, Massรกnyi P, Lukรกฤ N. Impact of oxidative stress on male fertility - a review.. Acta Vet Hung 2011 Dec;59(4):465-84.
    doi: 10.1556/avet.2011.034pubmed: 22079708google scholar: lookup
  27. Zhang L, Wang Y, Sohail T, Kang Y, Niu H, Sun X, Ji D, Li Y. Effects of Taurine on Sperm Quality during Room Temperature Storage in Hu Sheep.. Animals (Basel) 2021 Sep 18;11(9).
    doi: 10.3390/ani11092725pmc: PMC8470579pubmed: 34573691google scholar: lookup
  28. Ponraj P, Khate K, Vupru K. Taurine in semen extender modulates post-thaw semen quality, sperm kinematics and oxidative stress status in mithun (Bos frontalis) spermatozoa.. Asian Pacific J. Reprod. 2022;11:35โ€“43.
    doi: 10.4103/2305-0500.335860google scholar: lookup
  29. Chavda B.P, Vala K.B, Solanki G.B, Prajapati S.G, Singh V.K. Caffeine Supplementation in Semen Extender Protects Buffalo Spermatozoa from Cryodamage.. Indian J. Vet. Sci. Biotechnol. 2022;18:1โ€“5.
    doi: 10.48165/ijvsbt.18.4.01google scholar: lookup
  30. Ramirez-Perez H, Guerrero-Netro HM, Torres-Rodrรญguez P, Dรญaz-Durรกn M, Boeta-Acosta AM, Diaw M. A combination of taurine and caffeine maintains sperm quality in equine semen during chilled storage.. J Adv Vet Anim Res 2021 Dec;8(4):635-641.
    pmc: PMC8757662pubmed: 35106304doi: 10.5455/javar.2021.h555google scholar: lookup
  31. Alomar M. Effects of oxidized glutathione, cysteine and taurine supplementations on motility charateristics of different goat spermatozoa types.. Iran. J. Vet. Sci. Technol. 2021;13:84โ€“92.
  32. Rota A, Sabatini C, Przybyล‚ A, Ciaramelli A, Panzani D, Camillo F. Post-thaw Addition of Caffeine and/or Pentoxifylline Affect Differently Motility of Horse and Donkey-Cryopreserved Spermatozoa.. J Equine Vet Sci 2019 Apr;75:41-47.
    doi: 10.1016/j.jevs.2019.01.003pubmed: 31002091google scholar: lookup
  33. Slanina T, Miลกkeje M, Tirpรกk F, Bล‚aszczyk M, Formicki G, Massรกnyi P. Caffeine strongly improves motility parameters of turkey spermatozoa with no effect on cell viability.. Acta Vet Hung 2018 Mar;66(1):137-150.
    doi: 10.1556/004.2018.013pubmed: 29580077google scholar: lookup
  34. Kumar R, Singh VK, Chhillar S, Atreja SK. Effect of supplementation of taurine or trehalose in extender on immunolocalization of tyrosine phosphoproteins in buffalo and cattle (Karan Fries) cryopreserved spermatozoa.. Reprod Domest Anim 2013 Jun;48(3):407-15.
    doi: 10.1111/rda.12088pubmed: 23106450google scholar: lookup
  35. Paรกl D, Strejcek F, Tvrdรก E, Formicki G, Klein S, Rath D, Massรกnyi P. The in vitro effect of taurine on boar spermatozoa quality.. Acta Univ. Agric. Silvic. Mendel. Brun. 2018;66:131โ€“137.
    doi: 10.11118/actaun201866010131google scholar: lookup

Citations

This article has been cited 2 times.
  1. Halo M Jr, Kirchner R, Tirpรกk F, Slanina T, Tokรกrovรก K, Kovรกฤik A, Miลกkeje M, Greล„ A, Formicki G, Halo M, Madeddu R, Massรกnyi P. Essential and risk elements in horses affect haematology, serum biochemistry and oxidative status parameters. Sci Rep 2025 Jan 28;15(1):3489.
    doi: 10.1038/s41598-025-87908-6pubmed: 39875518google scholar: lookup
  2. Halo M Jr, Tirpรกk F, Massรกnyi M, Dianovรก L, Lenickรฝ M, Slanina T, Zemanovรก J, Matuลกkovรก A, Greล„ A, Halo M, Massรกnyi P. Calcium affects stallion spermatozoa parameters in different incubation temperatures. Heliyon 2024 Aug 30;10(16):e35879.
    doi: 10.1016/j.heliyon.2024.e35879pubmed: 39220990google scholar: lookup
Subscribe to our newsletter
Join 107,006 horse owners like you who receive our email updates on horse health and nutrition.
OmegaPrime+ Extra-Strength Omega-3 Oil - Mad Barn
NEW PRODUCT
OmegaPrime+
Extra-Strength Omega-3 Oil
Shop Now
ร—