A detection tube for cholinesterase inhibiting compounds.
Abstract: The enzyme butyrylcholinesterase from horse serum catalyses the hydrolysis of certain esters. The orange-red 2,6-dichloroindophenyl acetate will be converted by the enzyme into a deep blue alcohol. The colour transformation does not occur when the enzyme is inactivated. By making use of this biochemical reaction a cheap and simple, but very sensitive and specific detection tube could bedeveloped. The tube comprises a breakable ampoule with an aqueous buffer solution, a freeze-dried preparation of the chromogenic ester with a filler promoting its dissolution, a freeze-dried preparation of butyrylcholinesterase with a filler promoting its stability, and an indication layer. DDVP can be detected at concentrations as low as 0.4 mg/m3, when the sampled airvolume is 21.
Publication Date: 1979-01-01 PubMed ID: 573244DOI: 10.1080/03067317908071163Google Scholar: Lookup
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- Journal Article
- Analytical Methods
- Biochemistry
- Bioinformatics
- Biotechnology
- Clinical Pathology
- Diagnosis
- Diagnostic Technique
- Disease Diagnosis
- Disease Prevention
- Disease Surveillance
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- Enzymes
- Equine Health
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Summary
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This research focuses on the development of a cost-effective and simple detection tube that can identify cholinesterase inhibiting compounds. The new mechanism uses a specific biochemical reaction, which is both highly sensitive and specific, for detection.
Overview of the Research
The research paper revolves around the construction of a detection tube capable of identifying cholinesterase inhibiting compounds, primarily through the use of an enzyme called butyrylcholinesterase sourced from horse serum.
- The paper starts by explaining the role of butyrylcholinesterase, an enzyme that helps to break down, or hydrolyse, certain types of esters.
- Among the esters that this enzyme can break down is the orange-red coloured 2,6-dichloroindophenyl acetate. The enzyme is able to convert this ester into a deep blue alcohol.
- Moreover, it’s noted that when the enzyme is inactive, this colour transformation does not occur. This feature gives the ability for the detection process.
Construction of the Detection Tube
Next, the article delves into the details about the newly developed detection tube based on this biochemical process.
- The researchers created a tube that consists of a breakable ampoule filled with an aqueous buffer solution.
- The tube also contains a freeze-dried preparation of the chromogenic ester along with a filler that aids its dissolution process.
- Additionally, a freeze-dried preparation of butyrylcholinesterase is incorporated, paired with a filler that promotes its stability.
- The tube is also equipped with an indication layer that gives visual indication of detection.
Sensitivity of the Detection Tube
Lastly, the study focuses on the sensitivity and specificity of the developed system.
- The paper concludes by asserting that this detection tube is not only inexpensive and simple to construct, but it is also incredibly sensitive and specific in terms of the compounds it can detect.
- For instance, the DDVP compound can be detected at concentrations as low as 0.4 mg/m3, as long as the sampled air volume is above 21. This indicates the high sensitivity of the detection tube.
Cite This Article
APA
Barendsz AW.
(1979).
A detection tube for cholinesterase inhibiting compounds.
Int J Environ Anal Chem, 6(2), 89-94.
https://doi.org/10.1080/03067317908071163 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Butyrylcholinesterase / blood
- Cholinesterase Inhibitors / analysis
- Colorimetry
- Gelatin
- Horses
- Methods
Citations
This article has been cited 4 times.- Matějovský L, Pitschmann V. A Strip Biosensor with Guinea Green B and Fuchsin Basic Color Indicators on a Glass Nanofiber Carrier for the Cholinesterase Detection of Nerve Agents. ACS Omega 2019 Dec 17;4(25):20978-20986.
- Pitschmann V, Matějovský L, Lobotka M, Dědič J, Urban M, Dymák M. Modified Biosensor for Cholinesterase Inhibitors with Guinea Green B as the Color Indicator. Biosensors (Basel) 2018 Sep 4;8(3).
- Matějovský L, Pitschmann V. New Carrier Made from Glass Nanofibres for the Colorimetric Biosensor of Cholinesterase Inhibitors. Biosensors (Basel) 2018 May 30;8(2).
- Břízová A, Pitschmann V. Simple Chemical and Cholinesterase Methods for the Detection of Nerve Agents Using Optical Evaluation. Biosensors (Basel) 2023 Nov 22;13(12).
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