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Journal of global antimicrobial resistance2019; 20; 285-289; doi: 10.1016/j.jgar.2019.08.007

A link between the newly described colistin resistance gene mcr-9 and clinical Enterobacteriaceae isolates carrying blaSHV-12 from horses in Sweden.

Abstract: The aim of this study was to investigate the occurrence of the newly described transferable colistin resistance gene mcr-9 in extended-spectrum β-lactamase (ESBL)-producing clinical Enterobacteriaceae isolates from horses in Sweden. A total of 56 whole-genome sequenced ESBL-producing Enterobacteriaceae isolates from horses were subjected to in silico detection of antimicrobial resistance genes and identification of plasmid replicons types. The colistin minimum inhibitory concentration (MIC) for mcr-positive isolates was determined by broth microdilution. Relatedness between Enterobacteriaceae carrying mcr genes was determined by multilocus sequence typing (MLST) and core genome MLST. Thirty ESBL-producing Enterobacteriaceae isolates from horses were positive for the colistin resistance gene mcr-9. These isolates included Enterobacter cloacae, Escherichia coli, Klebsiella oxytoca and Citrobacter freundii and belonged to diverse MLST sequence types within each species. Two of the mcr-9-containing isolates originated from the same horse. All mcr-9-positive isolates had colistin MICs below or equal to the EUCAST epidemiological cut-off value of 2 mg/L and were negative for the two potential regulatory genes qseB-like and qseC-like for mcr-9. Except for one isolate carrying only bla, all of the isolates carried bla and bla, and were all considered multidrug-resistant as they harboured genes encoding resistance to aminoglycosides, chloramphenicol, fosfomycin, macrolides, quinolones, sulfonamides, trimethoprim and tetracyclines. Plasmid replicon types IncHI2 and IncHI2A were detected in all mcr-9-positive isolates. The occurrence of mcr-9 was common among clinical ESBL-producing Enterobacteriaceae isolates from horses in Sweden and was linked to the ESBL-encoding gene bla and plasmid replicon types IncHI2 and IncHI2A.
Copyright © 2019 The Author(s). Published by Elsevier Ltd.. All rights reserved.
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Publication Date: 2019-09-05 PubMed ID: 31494305DOI: 10.1016/j.jgar.2019.08.007Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research aimed to investigate the presence of the novel transferable gene mcr-9, responsible for colistin resistance, in Enterobacteriaceae isolates in horses in Sweden. The study found that this resistance gene was quite common and was often associated with the extended-spectrum β-lactamase (ESBL)-producing gene bla and replicon types IncHI2 and IncHI2A.

Objective of the Study

  • The aim of the study was to investigate the prevalence of the newly discovered transferable colistin resistance gene, mcr-9, in ESBL-producing clinical Enterobacteriaceae isolates from horses in Sweden.

Methods used in the Study

  • The researchers studied a total of 56 whole-genome sequenced ESBL–producing Enterobacteriaceae isolates from horses in Sweden.
  • The isolates were assessed using in silico detection to identify any antimicrobial resistance genes, as well as the presence of any types of plasmid replicons.
  • The minimum inhibitory concentration (MIC) of colistin for mcr-positive isolates was then determined using a broth microdilution technique.

Results of the Study

  • Thirty of the isolates from the horses were identified to be positive for the colistin resistance gene, mcr-9.
  • The researchers found that these isolates encompassed various species, including Enterobacter cloacae, Escerichia coli, etc. and belonged to diverse multilocus sequence types (MLST) within each species.
  • Except for one isolate carrying only bla, all of the isolates carried bla and bla, and were all considered multi-drug resistant as they harbored genes encoding resistance to multiple antibiotics.

Conclusion of the Study

  • The results showed that the occurrence of mcr-9 was commonplace in clinical ESBL-producing Enterobacteriaceae isolates in horses in Sweden.
  • These isolates are often associated with the ESBL-encoding gene blaSHV-12 and plasmid replicon types IncHI2 and IncHI2A.

Cite This Article

APA
Börjesson S, Greko C, Myrenås M, Landén A, Nilsson O, Pedersen K. (2019). A link between the newly described colistin resistance gene mcr-9 and clinical Enterobacteriaceae isolates carrying blaSHV-12 from horses in Sweden. J Glob Antimicrob Resist, 20, 285-289. https://doi.org/10.1016/j.jgar.2019.08.007

Publication

Journal of global antimicrobial resistance
ISSN: 2213-7173
NlmUniqueID: 101622459
Country: Netherlands
Language: English
Volume: 20
Pages: 285-289
PII: S2213-7165(19)30205-X

Researcher Affiliations

Börjesson, Stefan
  • Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), Uppsala, Sweden; Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden. Electronic address: stefan.borjesson@liu.se.
Greko, Christina
  • Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), Uppsala, Sweden.
Myrenås, Mattias
  • Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), Uppsala, Sweden.
Landén, Annica
  • Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), Uppsala, Sweden.
Nilsson, Oskar
  • Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), Uppsala, Sweden.
Pedersen, Karl
  • Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), Uppsala, Sweden.

MeSH Terms

  • Animals
  • Bacterial Proteins / genetics
  • Colistin / pharmacology
  • Computer Simulation
  • Drug Resistance, Multiple, Bacterial
  • Enterobacteriaceae / classification
  • Enterobacteriaceae / drug effects
  • Enterobacteriaceae / genetics
  • Enterobacteriaceae / isolation & purification
  • Enterobacteriaceae Infections / microbiology
  • Enterobacteriaceae Infections / veterinary
  • High-Throughput Nucleotide Sequencing
  • Horse Diseases / microbiology
  • Horses
  • Microbial Sensitivity Tests
  • Multilocus Sequence Typing
  • Plasmids / genetics
  • Sweden
  • Whole Genome Sequencing
  • beta-Lactamases / genetics

Citations

This article has been cited 33 times.
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