A link between the newly described colistin resistance gene mcr-9 and clinical Enterobacteriaceae isolates carrying blaSHV-12 from horses in Sweden.
Abstract: The aim of this study was to investigate the occurrence of the newly described transferable colistin resistance gene mcr-9 in extended-spectrum β-lactamase (ESBL)-producing clinical Enterobacteriaceae isolates from horses in Sweden. A total of 56 whole-genome sequenced ESBL-producing Enterobacteriaceae isolates from horses were subjected to in silico detection of antimicrobial resistance genes and identification of plasmid replicons types. The colistin minimum inhibitory concentration (MIC) for mcr-positive isolates was determined by broth microdilution. Relatedness between Enterobacteriaceae carrying mcr genes was determined by multilocus sequence typing (MLST) and core genome MLST. Thirty ESBL-producing Enterobacteriaceae isolates from horses were positive for the colistin resistance gene mcr-9. These isolates included Enterobacter cloacae, Escherichia coli, Klebsiella oxytoca and Citrobacter freundii and belonged to diverse MLST sequence types within each species. Two of the mcr-9-containing isolates originated from the same horse. All mcr-9-positive isolates had colistin MICs below or equal to the EUCAST epidemiological cut-off value of 2 mg/L and were negative for the two potential regulatory genes qseB-like and qseC-like for mcr-9. Except for one isolate carrying only bla, all of the isolates carried bla and bla, and were all considered multidrug-resistant as they harboured genes encoding resistance to aminoglycosides, chloramphenicol, fosfomycin, macrolides, quinolones, sulfonamides, trimethoprim and tetracyclines. Plasmid replicon types IncHI2 and IncHI2A were detected in all mcr-9-positive isolates. The occurrence of mcr-9 was common among clinical ESBL-producing Enterobacteriaceae isolates from horses in Sweden and was linked to the ESBL-encoding gene bla and plasmid replicon types IncHI2 and IncHI2A.
Copyright © 2019 The Author(s). Published by Elsevier Ltd.. All rights reserved.
Publication Date: 2019-09-05 PubMed ID: 31494305DOI: 10.1016/j.jgar.2019.08.007Google Scholar: Lookup
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Summary
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The research aimed to investigate the presence of the novel transferable gene mcr-9, responsible for colistin resistance, in Enterobacteriaceae isolates in horses in Sweden. The study found that this resistance gene was quite common and was often associated with the extended-spectrum β-lactamase (ESBL)-producing gene bla and replicon types IncHI2 and IncHI2A.
Objective of the Study
- The aim of the study was to investigate the prevalence of the newly discovered transferable colistin resistance gene, mcr-9, in ESBL-producing clinical Enterobacteriaceae isolates from horses in Sweden.
Methods used in the Study
- The researchers studied a total of 56 whole-genome sequenced ESBL–producing Enterobacteriaceae isolates from horses in Sweden.
- The isolates were assessed using in silico detection to identify any antimicrobial resistance genes, as well as the presence of any types of plasmid replicons.
- The minimum inhibitory concentration (MIC) of colistin for mcr-positive isolates was then determined using a broth microdilution technique.
Results of the Study
- Thirty of the isolates from the horses were identified to be positive for the colistin resistance gene, mcr-9.
- The researchers found that these isolates encompassed various species, including Enterobacter cloacae, Escerichia coli, etc. and belonged to diverse multilocus sequence types (MLST) within each species.
- Except for one isolate carrying only bla, all of the isolates carried bla and bla, and were all considered multi-drug resistant as they harbored genes encoding resistance to multiple antibiotics.
Conclusion of the Study
- The results showed that the occurrence of mcr-9 was commonplace in clinical ESBL-producing Enterobacteriaceae isolates in horses in Sweden.
- These isolates are often associated with the ESBL-encoding gene blaSHV-12 and plasmid replicon types IncHI2 and IncHI2A.
Cite This Article
APA
Börjesson S, Greko C, Myrenås M, Landén A, Nilsson O, Pedersen K.
(2019).
A link between the newly described colistin resistance gene mcr-9 and clinical Enterobacteriaceae isolates carrying blaSHV-12 from horses in Sweden.
J Glob Antimicrob Resist, 20, 285-289.
https://doi.org/10.1016/j.jgar.2019.08.007 Publication
Researcher Affiliations
- Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), Uppsala, Sweden; Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden. Electronic address: stefan.borjesson@liu.se.
- Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), Uppsala, Sweden.
- Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), Uppsala, Sweden.
- Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), Uppsala, Sweden.
- Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), Uppsala, Sweden.
- Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), Uppsala, Sweden.
MeSH Terms
- Animals
- Bacterial Proteins / genetics
- Colistin / pharmacology
- Computer Simulation
- Drug Resistance, Multiple, Bacterial
- Enterobacteriaceae / classification
- Enterobacteriaceae / drug effects
- Enterobacteriaceae / genetics
- Enterobacteriaceae / isolation & purification
- Enterobacteriaceae Infections / microbiology
- Enterobacteriaceae Infections / veterinary
- High-Throughput Nucleotide Sequencing
- Horse Diseases / microbiology
- Horses
- Microbial Sensitivity Tests
- Multilocus Sequence Typing
- Plasmids / genetics
- Sweden
- Whole Genome Sequencing
- beta-Lactamases / genetics
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