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Home / Equine Research Bank / Front Vet Sci / A New Approach of Sperm Motility Subpopulation Structure in ...
Frontiers in veterinary science2021; 8; 651477; doi: 10.3389/fvets.2021.651477

A New Approach of Sperm Motility Subpopulation Structure in Donkey and Horse.

Abstract: This study aimed to characterize the sperm kinematic values with high frames per second, to define the subpopulation structure of a horse and a donkey and compare them. A total of 57 fresh semen ejaculates (26 Spanish and 16 Arabian horse breeds and 10 donkeys) were collected and subsequently analyzed for kinematic parameters using the Computer-aided sperm motility analysis ISAS®v1.2 system and using a Spermtrack® 10-μm depth counting chamber. Sequences were recorded at 250 frames per second, and eight kinematic parameters were automatically evaluated. All kinematic parameters showed significant differences between a donkey and a horse and between horse breeds. All ejaculates evaluated showed excellent semen motility characteristics, with significantly higher values for all kinematic parameters for donkeys compared with horses except for beat-cross frequency. Donkey sperm was faster and linear than the horse. Regarding horse breeds differences, the Spanish horse had higher average path velocity, curvilinear velocity, and beat-cross frequency compared with the Arabian horse. Spanish horse sperm was rapid, but Arab horse was more linear. The principal component analysis showed three sperm subpopulations in the ejaculate of donkeys and horses with a significantly different motility characteristic between them. The dominant subpopulation for both donkey and horse was for rapid, straight, and linear with a high beat sperm (38.2 and 41.7%, respectively), whereas the lowest subpopulation was for the slowest and non-linear sperms. This, plus slight differences in the distribution of these subpopulations between Arabian and Spanish horses, were found. In conclusion, higher frames permitted to have a new interpretation of motile subpopulations with species and breed differences. More so, future works on donkey and horse breed spermatozoa should take into account differences between breeds that may interfere and alter the real analysis performed.
Copyright © 2021 Gacem, Valverde, Catalán, Yánez Ortiz, Soler and Miró.
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Publication Date: 2021-05-25 PubMed ID: 34113670PubMed Central: PMC8186528DOI: 10.3389/fvets.2021.651477Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the differences in sperm kinematics, their characteristics and subpopulations in horses and donkeys. The study found significant differences between these animals and also between different breeds of horses.

Methodology

  • The study collected 57 fresh semen samples. These were taken from 26 Spanish and 16 Arabian horse breeds, in addition to 10 donkeys.
  • The kinematic parameters of the sperm were analyzed using the ISAS®v1.2 system and a Spermtrack® 10-μm depth counting chamber.
  • The sequences were recorded at a rate of 250 frames per second.
  • The study evaluated eight kinematic parameters.

Findings

  • Significant differences were discovered in all kinematic parameters between horses and donkeys and between the different horse breeds.
  • The sperm of donkeys was found to be faster and more linear than horse sperm, with higher values for all kinematic parameters, except for beat-cross frequency.
  • The study also found that sperm from Spanish horses had a higher average path velocity, curvilinear velocity, and beat-cross frequency compared with Arabian horses.
  • The principal component analysis revealed three sperm subpopulations in the ejaculates of donkeys and horses, with significant motility differences between them.
  • The majority subpopulation for both donkeys and horses was rapid, straight, and linear sperm with a high beat(a characteristic of sperm to move in a certain pattern).
  • The least common subpopulation contained the slowest and non-linear sperms.

Conclusion

  • The use of high frame rate allowed new insights into the mobile subpopulations of sperm with respect to species and breed differences.
  • The authors concluded that future research should consider breed-specific differences, which could impact, and potentially distort, the results of the analysis.

Cite This Article

APA
Gacem S, Valverde A, Catalán J, Yánez Ortiz I, Soler C, Miró J. (2021). A New Approach of Sperm Motility Subpopulation Structure in Donkey and Horse. Front Vet Sci, 8, 651477. https://doi.org/10.3389/fvets.2021.651477

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 8
Pages: 651477

Researcher Affiliations

Gacem, Sabrina
  • Equine Reproduction Service, Autonomous University of Barcelona, Department of Animal Medicine and Surgery Veterinary Faculty, Catalonia, Spain.
Valverde, Anthony
  • Costa Rica Institute of Technology, School of Agronomy, Alajuela, Costa Rica.
Catalán, Jaime
  • Equine Reproduction Service, Autonomous University of Barcelona, Department of Animal Medicine and Surgery Veterinary Faculty, Catalonia, Spain.
Yánez Ortiz, Iván
  • Equine Reproduction Service, Autonomous University of Barcelona, Department of Animal Medicine and Surgery Veterinary Faculty, Catalonia, Spain.
Soler, Carles
  • Universitat de València, Departamento de Biología Celular, Biología Funcional y Antropología Física, Burjassot, Valencia, Spain.
Miró, Jordi
  • Equine Reproduction Service, Autonomous University of Barcelona, Department of Animal Medicine and Surgery Veterinary Faculty, Catalonia, Spain.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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