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Home / Equine Research Bank / J Gen Virol / A novel murine model for evaluating bovine papillomavirus pr...
The Journal of general virology2015; 96(9); 2764-2768; doi: 10.1099/vir.0.000212

A novel murine model for evaluating bovine papillomavirus prophylactics/therapeutics for equine sarcoid-like tumours.

Abstract: Equine sarcoids are highly recurrent bovine papillomavirus (BPV)-induced fibroblastic neoplasms that are the most common skin tumours in horses. In order to facilitate the study of potential equine sarcoid prophylactics or therapeutics, which can be a slow and costly process in equines, a murine model for BPV-1 protein-expressing equine sarcoid-like tumours was developed in mice through stable transfection of BPV-1 E5 and E6 in a murine fibroblast tumour cell line (K-BALB). Like equine sarcoids, these murine tumour cells (BPV-KB) were of fibroblast origin, were tumorigenic and expressed BPV-1 proteins. As an initial investigation of the preclinical potential of this tumour model for equine sarcoids prophylactics, mice were immunized with BPV-1 E5E6 Venezuelan equine encephalitis virus replicon particles, prior to BPV-KB challenge, which resulted in an increased tumour-free period compared with controls, indicating that the BPV-KB murine model may be a valuable preclinical alternative to equine clinical trials.
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Publication Date: 2015-06-04 PubMed ID: 26044793PubMed Central: PMC4635499DOI: 10.1099/vir.0.000212Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • N.I.H.
  • Extramural
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study is about developing a mouse model of equine sarcoid tumours to test potential treatments or prevention methods for this common horse skin cancer, which is caused by the bovine papillomavirus (BPV).

Background of the Study

  • The study addresses the topic of equine sarcoids, a common form of skin tumours in horses.
  • These tumours are caused by a form of bovine papillomavirus (BPV).
  • Previous studies and efforts to find a cure or preventative measure for these tumours have been slow and expensive due to the complexity of the condition and the difficulties in conducting trials on horses.

Objective of the Research

  • The paper presents a novel approach to studying potential treatments or prophylactics for equine sarcoid tumours, using a BPV-induced murine (mouse) model.
  • The goal of creating this model was to facilitate cheaper and quicker preclinical trials.

Methods

  • The researchers created the murine model by stably transfecting BPV-1 E5 and E6, proteins associated with the virus, into a mouse fibroblast tumour cell line known as K-BALB.
  • The transfection resulted in the formation of murine tumour cells, BPV-KB, which were similar to equine sarcoids in that they were of fibroblast origin and expressed BPV-1 proteins.

Findings

  • Having developed this murine model, the researchers then conducted a preliminary investigation of its preclinical potential against equine sarcoids prophylactics.
  • In this investigation, mice were immunized with BPV-1 E5E6 Venezuelan equine encephalitis virus replicon particles prior to being challenged with BPV-KB.
  • The results showed that this approach led to an increased tumour-free period in the immunized mice compared with controls.
  • This indicates that the murine model may offer a viable preclinical alternative to equine clinical trials for equine sarcoids prophylactics and potential therapeutics.

Cite This Article

APA
Bogaert L, Woodham AW, Da Silva DM, Martens A, Meyer E, Kast WM. (2015). A novel murine model for evaluating bovine papillomavirus prophylactics/therapeutics for equine sarcoid-like tumours. J Gen Virol, 96(9), 2764-2768. https://doi.org/10.1099/vir.0.000212

Publication

The Journal of general virology
ISSN: 1465-2099
NlmUniqueID: 0077340
Country: England
Language: English
Volume: 96
Issue: 9
Pages: 2764-2768

Researcher Affiliations

Bogaert, Lies
  • Norris Comprehensive Cancer Center, University of Southern California, NRT 7507, 1450 Biggy Street, Los Angeles, CA 90033, USA.
  • Department of Surgery and Anesthesiology of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Woodham, Andrew W
  • Norris Comprehensive Cancer Center, University of Southern California, NRT 7507, 1450 Biggy Street, Los Angeles, CA 90033, USA.
Da Silva, Diane M
  • Norris Comprehensive Cancer Center, University of Southern California, NRT 7507, 1450 Biggy Street, Los Angeles, CA 90033, USA.
  • Departments of Molecular Microbiology & Immunology and Obstetrics & Gynecology, University of Southern California, NRT 7507, 1450 Biggy Street, Los Angeles, CA 90033, USA.
Martens, Ann
  • Department of Surgery and Anesthesiology of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Meyer, Evelyne
  • Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Kast, W Martin
  • Departments of Molecular Microbiology & Immunology and Obstetrics & Gynecology, University of Southern California, NRT 7507, 1450 Biggy Street, Los Angeles, CA 90033, USA.
  • Norris Comprehensive Cancer Center, University of Southern California, NRT 7507, 1450 Biggy Street, Los Angeles, CA 90033, USA.

MeSH Terms

  • Animals
  • Bovine papillomavirus 1 / genetics
  • Bovine papillomavirus 1 / physiology
  • Disease Models, Animal
  • Horse Diseases / prevention & control
  • Horse Diseases / therapy
  • Horse Diseases / virology
  • Horses
  • Mice
  • Papillomavirus Infections / prevention & control
  • Papillomavirus Infections / therapy
  • Papillomavirus Infections / veterinary
  • Papillomavirus Infections / virology
  • Skin Neoplasms / prevention & control
  • Skin Neoplasms / therapy
  • Skin Neoplasms / veterinary
  • Skin Neoplasms / virology

Grant Funding

  • R01 CA074397 / NCI NIH HHS
  • TL1 TR000132 / NCATS NIH HHS
  • TL1TR000132 / NCATS NIH HHS

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Citations

This article has been cited 3 times.
  1. Podstawski P, Samiec M, Skrzyszowska M, Szmatoła T, Semik-Gurgul E, Ropka-Molik K. The Induced Expression of BPV E4 Gene in Equine Adult Dermal Fibroblast Cells as a Potential Model of Skin Sarcoid-like Neoplasia. Int J Mol Sci 2022 Feb 10;23(4).
    doi: 10.3390/ijms23041970pubmed: 35216085google scholar: lookup
  2. Kattner P, Zeiler K, Herbener VJ, Ferla-Brühl K, Kassubek R, Grunert M, Burster T, Brühl O, Weber AS, Strobel H, Karpel-Massler G, Ott S, Hagedorn A, Tews D, Schulz A, Prasad V, Siegelin MD, Nonnenmacher L, Fischer-Posovszky P, Halatsch ME, Debatin KM, Westhoff MA. What Animal Cancers teach us about Human Biology. Theranostics 2021;11(14):6682-6702.
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  3. Christensen ND, Budgeon LR, Cladel NM, Hu J. Recent advances in preclinical model systems for papillomaviruses. Virus Res 2017 Mar 2;231:108-118.
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