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Journal of equine veterinary science2021; 99; 103406; doi: 10.1016/j.jevs.2021.103406

A Novel Organic Composite Accelerates Wound Healing: Experimental and Clinical Study in Equine.

Abstract: Natural products such as honey, rosemary and chamomile oils have many health benefits particularly skin regeneration. These products were previously examined individually as enhancer of skin wound healing. The purpose of this study was to evaluate the clinical and histopathological features associated with using a mixture of rosemary and chamomile oils with honey, as a composite, in healing of equine-skin wounds experimentally and clinically. For experimental and clinical evaluation 15 donkeys and 122 clinical cases were used, respectively. For the experimental part, animals were divided into five equal groups. In each animal one skin wound was created bilaterally on the back region. All left-side wounds were treated with saline. Right-side wounds were treated with one of the following treatments, gentamycin, zinc, Kruuse Manuka, Revan and the composite ointments. Each wound treated once daily until one of the wounds healed completely. wounds were evaluated grossly and histopathologically. In clinical cases, wounds were dressed with the composite once daily and monitored until healing. Experimentally induced wounds treated with the composite were healed firstly, while all other wounds were still opened. Histologically, the skin of the composite treated wounds regenerated completely and efficiently. While the skin of the other wounds, showing incomplete regeneration of epidermis and dermis. Under field condition, the composite accelerates healing of different forms of equine wounds. The tested composite induce efficient and rapid skin regeneration.
Publication Date: 2021-02-09 PubMed ID: 33781408DOI: 10.1016/j.jevs.2021.103406Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

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The research article investigates the wound healing potential of a natural composite mixture made up of rosemary and chamomile oils, along with honey, on equine-skin wounds. The study concludes that the composite mixture accelerates wound healing efficiently and rapidly.

Methodology

  • The study used two sections for evaluation: an experimental approach with donkeys and a clinical application with over a hundred equine subjects.
  • In the experimental part, each donkey had a skin wound introduced on both sides of its back. All left-sided wounds were treated with saline, while right-sided wounds were treated with different substances: gentamycin, zinc, Kruuse Manuka, Revan, and the rosemary-chamomile oils composite.
  • Each wound was treated daily until one wound healed completely.
  • For clinical evaluation, different forms of equine wounds were dressed with the composite mixture daily and monitored until healing.

Findings

  • Experimentally, wounds treated with the rosemary-chamomile oils and honey composite healed first compared to other treatments.
  • Upon histological examination, the composite induced complete and efficient skin regeneration, while other treatments showed incomplete regeneration of the epidermis and dermis.
  • In clinical cases, the composite mixture accelerated the healing of different forms of equine wounds.
  • Thus, the rosemary-chamomile oils and honey composite induced efficient and rapid skin regeneration in both experimental and clinical implementation.

Significance

  • The research suggests that the tested composite is a promising natural treatment in accelerating wound healing.
  • The composite holds potential to be a preferred treatment option to enhance skin regeneration in veterinary practices, especially for equine injuries.
  • The study also reinforces the healing potential of natural products such as rosemary and chamomile oils, and honey, particularly for skin regeneration.

Cite This Article

APA
Anis A, Sharshar A, Hanbally SE, Sadek Y. (2021). A Novel Organic Composite Accelerates Wound Healing: Experimental and Clinical Study in Equine. J Equine Vet Sci, 99, 103406. https://doi.org/10.1016/j.jevs.2021.103406

Publication

ISSN: 0737-0806
NlmUniqueID: 8216840
Country: United States
Language: English
Volume: 99
Pages: 103406

Researcher Affiliations

Anis, Anis
  • Department of Pathology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt. Electronic address: aniszaid@vet.usc.edu.eg.
Sharshar, Ahmed
  • Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
Hanbally, Saber El
  • Department of Pharmacology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt.
Sadek, Yasmin
  • Department of Pathology, Animal Health Institute, Giza, Egypt.

MeSH Terms

  • Animals
  • Epidermis
  • Honey
  • Horses
  • Plant Extracts
  • Skin
  • Wound Healing

Citations

This article has been cited 7 times.
  1. Busto F, Licini C, Cometa S, Liotino S, Damiani E, Bacchetti T, Kleider I, La Contana A, Mattioli-Belmonte M, De Giglio E. Pectin/Gellan Gum Hydrogels Loaded with Crocus sativus Tepal Extract for In Situ Modulation of Pro-Inflammatory Pathways Affecting Wound Healing. Polymers (Basel) 2025 Mar 20;17(6).
    doi: 10.3390/polym17060814pubmed: 40292702google scholar: lookup
  2. Zainuddin ANZ, Mustakim NN, Rosemanzailani FA, Fadilah NIM, Maarof M, Fauzi MB. A Comprehensive Review of Honey-Containing Hydrogel for Wound Healing Applications. Gels 2025 Mar 12;11(3).
    doi: 10.3390/gels11030194pubmed: 40136899google scholar: lookup
  3. Demyashkin G, Sataieva T, Shevkoplyas L, Kuevda T, Ahrameeva M, Parshenkov M, Mimuni A, Pimkin G, Atiakshin D, Shchekin V, Shegay P, Kaprin A. Burn Wound Healing Activity of Hydroxyethylcellulose Gels with Different Water Extracts Obtained from Various Medicinal Plants in Pseudomonas aeruginosa-Infected Rabbits. Int J Mol Sci 2024 Aug 18;25(16).
    doi: 10.3390/ijms25168990pubmed: 39201676google scholar: lookup
  4. Yasin SNN, Said Z, Halib N, Rahman ZA, Mokhzani NI. Polymer-Based Hydrogel Loaded with Honey in Drug Delivery System for Wound Healing Applications. Polymers (Basel) 2023 Jul 18;15(14).
    doi: 10.3390/polym15143085pubmed: 37514474google scholar: lookup
  5. Anis A, Sharshar A, El Hanbally S, Shehata AA. Histopathological Evaluation of the Healing Process of Standardized Skin Burns in Rabbits: Assessment of a Natural Product with Honey and Essential Oils. J Clin Med 2022 Oct 29;11(21).
    doi: 10.3390/jcm11216417pubmed: 36362645google scholar: lookup
  6. Helal IE, Al-Abbadi HA, El-Daharawy MH, Ahmed MF. Enhancement of chronic wound healing with maltodextrin/ascorbic acid gel: a clinical evaluation of distal limb wounds in horses. J Anim Sci Technol 2022 Sep;64(5):997-1007.
    doi: 10.5187/jast.2022.e52pubmed: 36287738google scholar: lookup
  7. Bahari N, Hashim N, Md Akim A, Maringgal B. Recent Advances in Honey-Based Nanoparticles for Wound Dressing: A Review. Nanomaterials (Basel) 2022 Jul 26;12(15).
    doi: 10.3390/nano12152560pubmed: 35893528google scholar: lookup