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Theriogenology2012; 79(5); 744-750; doi: 10.1016/j.theriogenology.2012.11.030

A novel strategy of mesenchymal stem cells delivery in the uterus of mares with endometrosis.

Abstract: Mesenchymal stem cells (MSCs), because of their immunomodulation and trophic activities, in addition to their capacity to regenerate damaged tissues, have potential for treatment of many diseases. The success of stem cell therapies depends, in part, on the method of cell delivery, which should provide wide cell distribution and homing in to injured sites. The objective of the present study was to developing a novel strategy for delivery of MSCs into the uterus of mares with endometrosis (degenerative alteration of uterine glands and surrounding stroma). Endometrosis was confirmed in all mares (N = 6) used in this study. To trace multipotent equine adipose tissue-derived MSCs (eAT-MSCs) in endometrial tissue, before transplantation, cells were stained with a fluorescent dye. During a synchronized estrus, the eAT-MSCs (2 × 10(7) diluted in 20 mL of sodium chloride 0.9%) were inoculated into uterus using a simple technique, similar to artificial insemination (AI) in mares. At 7 and 21 days after transplantation, homing of fluorescently labeled eAT-MSCs was observed by confocal microscopy of uterine biopsies collected from the uterine body and in both uterine horns, including glandular and periglandular spaces, in three of four treated mares. Herein, we propose a new method of MSCs delivery in uterus of mares with endometrosis, which was minimally invasive and technically simple.
Copyright © 2013. Published by Elsevier Inc.
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Publication Date: 2012-12-25 PubMed ID: 23270861DOI: 10.1016/j.theriogenology.2012.11.030Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focused on a new technique for delivering mesenchymal stem cells (MSCs) into the uterus of mare horses suffering from endometrosis, a condition involving the degeneration of uterine glands and tissue around them. The researchers traced the MSCs derived from horse adipose tissue in the uterine tissue and observed successful homing of the cells in the uterine biopsies of three out of four treated mares.

Research Methodology

  • The researchers used six mares with confirmed endometrosis in this study.
  • Equine adipose tissue-derived mesenchymal stem cells (eAT-MSCs) were used. These cells were first stained with a fluorescent dye to trace them in the endometrial tissue after transplantation.
  • During a synchronized estrus (a recurring period of sexual receptivity and fertility in many female mammals), the eAT-MSCs were delivered into the uterus. The cells were diluted in sodium chloride and inoculated using a technique similar to artificial insemination (AI) in mares.

Results and Observations

  • At seven and twenty-one days post transplantation, the researchers examined uterine biopsies from the mares.
  • With the aid of confocal microscopy, they were able to observe the homing of the fluorescently labeled eAT-MSCs.
  • The successfully homed cells were seen in the uterine body and in both uterine horns, including the glandular and periglandular spaces, in three of four treated mares.

Conclusions

  • Based on these observations, the researchers proposed this new method of MSCs delivery in the uterus of mares with endometrosis, indicating that it is minimally invasive and technically simple.
  • While the results were positive for most of the mares treated during the study, further research would be useful to fully explore the efficacy and potential of this new treatment technique.

Cite This Article

APA
Mambelli LI, Winter GH, Kerkis A, Malschitzky E, Mattos RC, Kerkis I. (2012). A novel strategy of mesenchymal stem cells delivery in the uterus of mares with endometrosis. Theriogenology, 79(5), 744-750. https://doi.org/10.1016/j.theriogenology.2012.11.030

Publication

Theriogenology
ISSN: 1879-3231
NlmUniqueID: 0421510
Country: United States
Language: English
Volume: 79
Issue: 5
Pages: 744-750

Researcher Affiliations

Mambelli, L I
  • Laboratório de Genética, Instituto Butantan, São Paulo, São Paulo, Brazil. lis_mambelli@yahoo.com.br
Winter, G H Z
    Kerkis, A
      Malschitzky, E
        Mattos, R C
          Kerkis, I

            MeSH Terms

            • Animals
            • Cell Movement
            • Endometriosis / pathology
            • Endometriosis / therapy
            • Endometriosis / veterinary
            • Female
            • Horse Diseases / therapy
            • Horses
            • Mesenchymal Stem Cell Transplantation / methods
            • Mesenchymal Stem Cell Transplantation / veterinary
            • Mesenchymal Stem Cells
            • Uterus / transplantation

            Citations

            This article has been cited 18 times.
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