A pilot study of regenerative therapy by implanting synovium-derived mesenchymal stromal cells in equine osteochondral defect models.
Abstract: Synovium-derived mesenchymal stromal cells (SM-MSCs) from seven Thoroughbreds with naturally occurring intra-articular fracture proliferated to over ten million cells by the second passage. Using three experimental Thoroughbreds, columnar osteochondral defects were made arthroscopically at the bilateral distal radius. Five million allogenic SM-MSCs were implanted into the right defect, and another five million were injected into the right radio-carpal joint (implantation site). No SM-MSCs were implanted into the left defect or the same joint (control site). At 3 and 6 weeks after surgery, ten million autologous SM-MSCs were injected into the right joints. Radiolucent volumes of defects calculated by analysis of postmortem CT images 9 weeks after surgery were decreased in implanted sites compared with control sites in all horses. The average scores for ICRS gross and histopathological grading scales in implanted sites were equal to or higher than those of the controls. These results suggest that allogenic implantation and subsequent autologous injection of SM-MSCs might not obstruct subchondral bone formation in defects.
Publication Date: 2018-12-18 PubMed ID: 30607136PubMed Central: PMC6306295DOI: 10.1294/jes.29.117Google Scholar: Lookup
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- Journal Article
Summary
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This research examined the possibility of using equine synovium-derived mesenchymal stromal cells (SM-MSCs) for regenerative therapy to treat osteochondral defects in horses. The results suggested a decrease in radiolucent volumes of defects after SM-MSCs were implanted and autologous cells were injected, which may indicate potential for successful bone formation.
Methodology
- The study involved a total of seven Thoroughbred horses that demonstrated naturally occurring intra-articular fractures.
- SM-MSCs were derived from the horses’ synovial fluid and allowed to proliferate up to the second passage, by when their numbers increased to over ten million.
- Three of the Thoroughbreds were used to create experimental models. Here, columnar osteochondral defects were made in the bilateral distal radius of each horse via arthroscopy.
- To offer a comparative study, only the right defect, and its corresponding joint (right radio-carpal) were used to implant five million allogenic SM-MSCs each. The left defect and corresponding joint were left untreated, acting as controls.
- The process was then followed by autologous injections of ten million SM-MSCs into the right joints after three and six weeks of surgery.
Results
- The healing progress was monitored at 3, 6 and 9 weeks post-surgery using postmortem CT images for calculation of radiolucent volumes of the defects.
- The radiolucent volumes decreased in the treated defects compared to the untreated defects in all the horses, suggesting successful bone formation.
- The treated sites also showed equal or higher scores in the gross and histopathological grading under the International Cartilage Repair Society (ICRS) standards, confirming that the treated sites had better healing compared to the untreated sites.
Conclusion
- The successful implantation and injection of SM-MSCs clearly suggest that this regenerative therapy could be a beneficial treatment way for equine osteochondral defects.
- The therapy does not obstruct subchondral bone formation in the defects, reinforcing the conclusion that SM-MSCs hold potential for successful tissue regeneration and repair.
Cite This Article
APA
Yamasaki A, Omura T, Murata D, Kobayashi M, Sunaga T, Kusano K, Ueno Y, Kuramoto T, Hobo S, Misumi K.
(2018).
A pilot study of regenerative therapy by implanting synovium-derived mesenchymal stromal cells in equine osteochondral defect models.
J Equine Sci, 29(4), 117-122.
https://doi.org/10.1294/jes.29.117 Publication
Researcher Affiliations
- Department of Veterinary Clinical Science, Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
- Racehorse Hospital, Miho Training Center, Japan Racing Association, Ibaragi 300-0493, Japan.
- Department of Veterinary Clinical Science, Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
- Racehorse Hospital, Miho Training Center, Japan Racing Association, Ibaragi 300-0493, Japan.
- Department of Veterinary Clinical Science, Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
- Racehorse Hospital, Miho Training Center, Japan Racing Association, Ibaragi 300-0493, Japan.
- Racehorse Hospital, Miho Training Center, Japan Racing Association, Ibaragi 300-0493, Japan.
- Department of Veterinary Clinical Science, Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
- Department of Veterinary Clinical Science, Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
- Department of Veterinary Clinical Science, Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
References
This article includes 15 references
- Arnhold SJ, Goletz I, Klein H, Stumpf G, Beluche LA, Rohde C, Addicks K, Litzke LF. Isolation and characterization of bone marrow-derived equine mesenchymal stem cells.. Am J Vet Res 2007 Oct;68(10):1095-105.
- Braun J, Hack A, Weis-Klemm M, Conrad S, Treml S, Kohler K, Walliser U, Skutella T, Aicher WK. Evaluation of the osteogenic and chondrogenic differentiation capacities of equine adipose tissue-derived mesenchymal stem cells.. Am J Vet Res 2010 Oct;71(10):1228-36.
- Davatchi F, Sadeghi Abdollahi B, Mohyeddin M, Nikbin B. Mesenchymal stem cell therapy for knee osteoarthritis: 5 years follow-up of three patients.. Int J Rheum Dis 2016 Mar;19(3):219-25.
- Fülber J, Maria DA, da Silva LC, Massoco CO, Agreste F, Baccarin RY. Comparative study of equine mesenchymal stem cells from healthy and injured synovial tissues: an in vitro assessment.. Stem Cell Res Ther 2016 Mar 5;7:35.
- Murata D, Miyakoshi D, Hatazoe T, Miura N, Tokunaga S, Fujiki M, Nakayama K, Misumi K. Multipotency of equine mesenchymal stem cells derived from synovial fluid.. Vet J 2014 Oct;202(1):53-61.
- Murata D, Tokunaga S, Tamura T, Kawaguchi H, Miyoshi N, Fujiki M, Nakayama K, Misumi K. A preliminary study of osteochondral regeneration using a scaffold-free three-dimensional construct of porcine adipose tissue-derived mesenchymal stem cells.. J Orthop Surg Res 2015 Mar 18;10:35.
- Murata D, Yamasaki A, Matsuzaki S, Sunaga T, Fujiki M, Tokunaga S, Misumi K. Characteristics and multipotency of equine dedifferentiated fat cells.. J Equine Sci 2016;27(2):57-65.
- Nakamura T, Sekiya I, Muneta T, Kobayashi E. [Articular cartilage regenerative therapy with synovial mesenchymal stem cells in a pig model].. Clin Calcium 2013 Dec;23(12):1741-9.
- Prado AA, Favaron PO, da Silva LC, Baccarin RY, Miglino MA, Maria DA. Characterization of mesenchymal stem cells derived from the equine synovial fluid and membrane.. BMC Vet Res 2015 Nov 10;11:281.
- Sekiya I, Muneta T, Horie M, Koga H. Arthroscopic Transplantation of Synovial Stem Cells Improves Clinical Outcomes in Knees With Cartilage Defects.. Clin Orthop Relat Res 2015 Jul;473(7):2316-26.
- Shimomura K, Ando W, Tateishi K, Nansai R, Fujie H, Hart DA, Kohda H, Kita K, Kanamoto T, Mae T, Nakata K, Shino K, Yoshikawa H, Nakamura N. The influence of skeletal maturity on allogenic synovial mesenchymal stem cell-based repair of cartilage in a large animal model.. Biomaterials 2010 Nov;31(31):8004-11.
- Suzuki S, Muneta T, Tsuji K, Ichinose S, Makino H, Umezawa A, Sekiya I. Properties and usefulness of aggregates of synovial mesenchymal stem cells as a source for cartilage regeneration.. Arthritis Res Ther 2012 Jun 7;14(3):R136.
- Toupadakis CA, Wong A, Genetos DC, Cheung WK, Borjesson DL, Ferraro GL, Galuppo LD, Leach JK, Owens SD, Yellowley CE. Comparison of the osteogenic potential of equine mesenchymal stem cells from bone marrow, adipose tissue, umbilical cord blood, and umbilical cord tissue.. Am J Vet Res 2010 Oct;71(10):1237-45.
- Vidal MA, Robinson SO, Lopez MJ, Paulsen DB, Borkhsenious O, Johnson JR, Moore RM, Gimble JM. Comparison of chondrogenic potential in equine mesenchymal stromal cells derived from adipose tissue and bone marrow.. Vet Surg 2008 Dec;37(8):713-24.
- Violini S, Ramelli P, Pisani LF, Gorni C, Mariani P. Horse bone marrow mesenchymal stem cells express embryo stem cell markers and show the ability for tenogenic differentiation by in vitro exposure to BMP-12.. BMC Cell Biol 2009 Apr 22;10:29.
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